Effectiveness of a Toothpaste with Low Fluoride Content Combined with Trimetaphosphate on Dental Biofilm and Enamel Demineralization in situ

2015 ◽  
Vol 49 (4) ◽  
pp. 394-400 ◽  
Author(s):  
Eliana M. Takeshita ◽  
Marcelle Danelon ◽  
Luciene P. Castro ◽  
Kikue T. Sassaki ◽  
Alberto C.B. Delbem
Keyword(s):  
Sucrose Solution ◽  
Blind Study ◽  
Double Blind Study ◽  
Oral Appliances ◽  
Double Blind ◽  
Sodium Trimetaphosphate ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Mineral Loss

Objective: The aim of the present study was to evaluate in situ whether a toothpaste with low fluoride associated with sodium trimetaphosphate (TMP) would provide similar effect to that of a 1,100 ppm F toothpaste. Design: This crossover double-blind study consisted of 4 phases (14 days each), during which 10 volunteers wore oral appliances containing 4 enamel bovine blocks. The cariogenic challenge was performed by the application of a 20% sucrose solution (6×/day). The toothpaste treatments (2×/day) were: placebo, 500 ppm F, 500 ppm F plus 1% TMP, and 1,100 ppm F. At the end, enamel mineral loss and biofilm composition were analyzed. Results: The toothpaste with 500 ppm F plus 1% TMP showed the lowest mineral loss (p < 0.05). Regarding the fluoride and calcium concentrations in the enamel and in the biofilm, there were no significant differences between 500 ppm F plus 1% TMP, and 1,100 ppm F toothpastes (p > 0.569), but they were significantly different when compared to toothpaste with 500 ppm F (p < 0.050). Conclusion: The addition of 1% TMP to a low-fluoride toothpaste reduces enamel demineralization in situ similar to a 1,100 ppm F toothpaste.

scholarly journals Effect of APF gel application time on enamel demineralization and fluoride uptake in situ

2009 ◽  
Vol 20 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Rita Sarmiento Villena ◽  
Livia Maria Andaló Tenuta ◽  
Jaime Aparecido Cury
Keyword(s):  
Dental Plaque ◽  
Sucrose Solution ◽  
Blind Study ◽  
Application Time ◽  
Treatment Groups ◽  
Fluoride Uptake ◽  
Time Of Application ◽  
Enamel Demineralization

This in situ crossover and blind study was conducted to investigate the effect of professional acidulated phosphate fluoride (APF) gel application time on the subsequent inhibition of enamel demineralization. During 3 phases of 28 days each, 15 volunteers wore palatal appliances containing 4 enamel blocks, which were subjected to 3 treatment groups: not treated (control) and pre-treated with APF gel for 1 or 4 min. Dental plaque was allowed to accumulate on the blocks and the appliances were immersed in 10% sucrose solution 3 times a day simulating a cariogenic challenge. After each phase, the blocks were removed to evaluate enamel demineralization and concentration of fluoride (F) remaining after the cariogenic challenge. F formed on enamel was determined in additional enamel blocks subjected only to APF gel application. APF gel was efficient in reducing enamel demineralization (p<0.05), irrespective of the application time (p>0.05). Also, the concentration of the F formed and retained on enamel was significantly higher after APF gel application (p<0.05), but the effect of time of application was not statistically significant (p>0.05). The results suggest that APF application for either 1 or 4 min is equally efficient to increase F concentration in enamel and reduce enamel demineralization.

In situ Mineral Loss Inhibition by CO2 Laser and Fluoride

2006 ◽  
Vol 85 (7) ◽  
pp. 617-621 ◽  
Author(s):  
L.K.A. Rodrigues ◽  
M. Nobre dos Santos ◽  
J.D.B. Featherstone
Keyword(s):  
Laser Irradiation ◽  
Laser Treatment ◽  
Co2 Laser ◽  
Sucrose Solution ◽  
Treatment Groups ◽  
Enamel Demineralization ◽  
Human Enamel ◽  
Fluoride Dentifrice ◽  
Mineral Loss

Laser and fluoride treatments have been shown to inhibit enamel demineralization in the laboratory. However, the intra-oral effects of this association have not been tested. This study assessed in situ the effect of a Transversely Excited Atmospheric CO2 laser (λ = 9.6 μm) and the use of pressure fluoridated dentifrice on enamel demineralization. During two 14-day phases, 17 volunteers wore palatal appliances containing human enamel slabs assigned to treatment groups, as follows: (1) non-fluoride dentifrice, (2) CO2 laser irradiation plus non-fluoride dentifrice, (3) fluoride dentifrice, and (4) CO2 laser irradiation plus fluoride dentifrice. A 20% sucrose solution was dripped onto the slabs 8 times per day. The specimens treated with laser and/or fluoridated dentifrice presented a significantly lower mineral loss when compared with those from the non-fluoride dentifrice group. The results suggested that CO2 laser treatment of enamel inhibits demineralization in the human mouth, being more effective when associated with fluoride.

scholarly journals In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries

2014 ◽  
Vol 15 (2) ◽  
pp. 142-148 ◽  
Author(s):  
Suyane Maria Luna Cruz de Vasconcelos ◽  
Mary Anne Sampaio de Melo ◽  
Joao Paulo Marques Saraiva Wenceslau ◽  
Iriana Carla Junqueira Zanin ◽  
Haroldo Cesar Pinheiro Beltrao ◽  
...  
Keyword(s):  
Mutans Streptococci ◽  
Adhesive Systems ◽  
Double Blind ◽  
Secondary Caries ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Significant Difference ◽  
In Situ Assessment ◽  
Self Etch

ABSTRACT Aim This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization. Materials and methods During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SETM (self-etch, fluoride/MDPB free adhesive, AB) and Clearfil Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S (ƒ¢S) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 ƒÊm from the restoration margin. Data were analyzed by ANOVA. Results No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm. Conclusion All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research. How to cite this article de Vasconcelos SMLC, de Melo MAS, Wenceslau JPMS, Zanin ICJ, Beltrao HCP, Fernandes CAO, de Almeida PC, Rodrigues LKA. In situ Assessment of Effects of the Bromide- and Fluoride-Incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014;15(2):142-148.

scholarly journals Effect of starch on the cariogenic potential of sucrose

2005 ◽  
Vol 94 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Cecilia C. C. Ribeiro ◽  
Cínthia P. M. Tabchoury ◽  
Altair A. Del Bel Cury ◽  
Livia M. A. Tenuta ◽  
Pedro L. Rosalen ◽  
...  
Keyword(s):  
Animal Studies ◽  
Amylase Activity ◽  
Negative Control ◽  
Cross Sectional ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Two Phases ◽  
Mineral Loss

Sincein vitroand animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessedin situthe effects of this association appliedin vitroon the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0·05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0·05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.

Toothpaste with Nanosized Trimetaphosphate Reduces Enamel Demineralization

2016 ◽  
Vol 2 (3) ◽  
pp. 233-240 ◽  
Author(s):  
M.D.B. Souza ◽  
J.P. Pessan ◽  
C.S. Lodi ◽  
J.A.S. Souza ◽  
E.R. Camargo ◽  
...  
Keyword(s):  
Protective Effect ◽  
Surface Hardness ◽  
Extracellular Polysaccharide ◽  
Degree Of Saturation ◽  
Double Blind ◽  
Cross Sectional ◽  
Fluoride Toothpaste ◽  
Sodium Trimetaphosphate ◽  
Enamel Demineralization

This double-blind crossover study assessed the effects of a low-fluoride (low-F) dentifrice containing nanosized sodium trimetaphosphate (TMP) on enamel demineralization in situ. Nineteen subjects wore palatal appliances containing 4 blocks of bovine enamel and were randomly assigned to brush their teeth with placebo (without F/TMP), 250-ppm F (250F), 250F plus 0.05% nanosized TMP (250F-TMPnano), and 1,100-ppm F (1,100F) dentifrices during 7 d, under cariogenic challenge. Enamel surface hardness and cross-sectional hardness (ΔKHN [Knoop hardness number]), as well as F, calcium (Ca), and phosphorus (P) concentrations, were determined. Also, biofilm that formed on the blocks was analyzed for F, Ca, P, and insoluble extracellular polysaccharide concentrations. Data were submitted to analysis-of-variance models and Student-Newman-Keuls test ( P < 0.05). The 250F-TMPnano dentifrice promoted the lowest ΔKHN among all groups ( P < 0.001), while the percentage of surface hardness loss was similar to 1,100F. Also, similar F, Ca, and P concentrations in enamel were observed for 1,100F and 250F-TMPnano. In the biofilm, the highest F content was observed for 1,100F; Ca content was similar between 1,100F and 250F-TMPnano; and P content was similar among all groups. Similar extracellular polysaccharide values were observed for 250F-TMPnano and 1,100F ( P < 0.001), ionic activity of CaHPO40, CaF+, and HF0 ( P < 0.05) and degree of saturation of hydroxyapatite and CaF2 ( P < 0.05). It was concluded that the protective effect of 250F-TMPnano dentifrice was similar to a conventional dentifrice for most of the variables studied, having a more pronounced effect on the subsurface lesion when compared with the conventional toothpaste (1,100F). Knowledge Transfer Statement: Although toothpastes containing ≥1,000-ppm fluoride are more effective than low-fluoride formulations against dental caries, their early use can lead to side effects. This has prompted intensive research on alternatives to increase the anticaries effect of low-fluoride toothpastes. The present in situ study demonstrated that the addition of sodium trimetaphosphate nanoparticles to toothpastes containing 250-ppm fluoride significantly enhances the protective effect of this formulation against enamel demineralization to levels comparable to a 1,100-ppm fluoride toothpaste in terms of most of the variables studied. Most important, this formulation promoted the lowest loss of subsurface hardness among all groups, suggesting that caries lesions would take longer to develop under clinical conditions when compared with a conventional (1,100-fluoride) toothpaste.

Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ

2015 ◽  
Vol 49 (5) ◽  
pp. 499-507 ◽  
Author(s):  
Beatriz M. Souza ◽  
Livia P. Comar ◽  
Mariele Vertuan ◽  
Constantino Fernandes Neto ◽  
Marília Afonso Rabelo Buzalaf ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Positive Influence ◽  
Double Blind Study ◽  
Double Blind ◽  
Mi Paste Plus ◽  
Mi Paste ◽  
Fluoride Dentifrice ◽  
Casein Phosphopeptide ◽  
Mineral Loss

This study evaluated the effect of an experimental paste containing hydroxyapatite in nanoparticles (nano-HA)/fluoride on dental de-remineralisation in situ. Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-demineralised specimens were worn intraorally at each phase corresponding to the following treatments: Nanop Plus (10% HA, 0.2% NaF, nano-HA/fluoride), MI Paste Plus (casein phosphopeptide-amorphous calcium phosphate, 0.2% NaF), F (0.2% NaF) and placebo. Two-hundred and forty enamel and 240 dentine specimens were selected by using surface microhardness; half of them were subjected to pre-demineralisation and the other half remained sound. Sound specimens were further exposed to severe cariogenic challenge (20% sucrose in biofilm) in situ, while pre-demineralised specimens were not. All specimens were exposed to fluoride dentifrice slurry 2 × 1 min/day. Thereafter, the treatments were done for 4 min. The de-remineralisation was quantified by transversal microradiography. The data were statistically analysed by repeated-measures ANOVA/Tukey's tests (p < 0.05). Generally, no huge differences were found among the treatments. However, Nanop Plus was the only treatment able to significantly reduce dentine demineralisation (ΔZ, integrated mineral loss) and to improve enamel remineralisation (ΔΔZ, integrated mineral uptake) compared to placebo. No treatments were able to reduce enamel demineralisation, while for dentine remineralisation all treatments were similarly effective in improving ΔΔZ compared to placebo. Nanop Plus seems to have a positive influence on dental de-remineralisation, which should be further confirmed.

In situ Effect of Frequent Sucrose Exposure on Enamel Demineralization and on Plaque Composition after APF Application and F Dentifrice Use

2004 ◽  
Vol 83 (1) ◽  
pp. 71-75 ◽  
Author(s):  
A.F. Paes Leme ◽  
R. Dalcico ◽  
C.P.M. Tabchoury ◽  
A.A. Del Bel Cury ◽  
P.L. Rosalen ◽  
...  
Keyword(s):  
Sucrose Solution ◽  
Experimental Period ◽  
Mutans Streptococci ◽  
Plaque Composition ◽  
Treatment Groups ◽  
Enamel Demineralization ◽  
Combination Of Methods ◽  
Bovine Enamel ◽  
Mineral Loss

Since the effect of the combination of methods of fluoride use on enamel demineralization and on plaque composition is not clearly established, this study examined the effect of the combination of acidulated phosphate fluoride (APF) application and F dentifrice on enamel demineralization and on plaque composition. In this crossover study, 16 volunteers, wearing a palatal appliance containing bovine enamel blocks, were subjected to 4 treatment groups: non-fluoridated dentifrice (PD), FD, APF+PD, and APF+FD. The APF was applied to the enamel before the 14-day experimental period. During the experimental period, test dentifrices were applied 3×/day, and a 20% sucrose solution was applied 4× and 8×/day by being dripped on the blocks. Although APF application was able either to increase F concentration in plaque or to reduce the % of mutans streptococci, its combination with F dentifrice use neither reduced enamel mineral loss nor changed any other measured plaque variable with respect to the FD group alone.

In situ Effect of Arginine-Containing Dentifrice on Plaque Composition and on Enamel Demineralization under Distinct Cariogenic Conditions

2018 ◽  
Vol 52 (6) ◽  
pp. 588-597 ◽  
Author(s):  
Angel Y. Sanchez ◽  
Caroline L. de Oliveira ◽  
Thais C. Negrini ◽  
Lina N. Hashizume ◽  
Anderson T. Hara ◽  
...  
Keyword(s):  
Sucrose Solution ◽  
Surface Hardness ◽  
Extracellular Polysaccharide ◽  
Experimental Phase ◽  
Enamel Demineralization ◽  
Bovine Enamel ◽  
Adult Volunteers ◽  
Mineral Loss ◽  
In Situ Split

There is limited evidence that arginine-containing fluoridated dentifrices (AFD) have a better anticaries effect than regular fluoridated dentifrices (FD), especially in subjects at a higher risk for caries development. This study aimed to assess the effect of AFD on enamel demineralization and on the microbial and biochemical compositions of biofilm formed under different frequencies of sucrose exposure. It consisted of an in situ split-mouth design, where 12 adult volunteers who used FD for at least 2 months prior to the beginning of this study wore acrylic palatal appliances containing 4 bovine enamel specimens (1 pair at each side of the appliance) during 2 phases of 14 days each. FD slurry (3×/day) and 20% sucrose solution (4× and 8×/day) were dripped on the specimens during the first experimental phase. The same volunteers then used AFD during a 2-month washout period, followed by a second experimental phase where the AFD slurry and sucrose solution were applied onto a new subset of specimens. The percentage of enamel surface hardness loss (%SHL), the lesion depth (LD), the integrated mineral loss (IML), microbial counts on biofilms, the biomass, and inorganic and insoluble extracellular polysaccharide (IEPS) biofilm concentrations were determined. Higher %SHL, biomass, and IEPS and lower fluoride values were found at sucrose 8×/day exposure. Lower IEPS were found in the presence of AFD compared to FD. Similar %SHL, LD, and IML values were found between FD and AFD, irrespectively of the cariogenic challenge. The results suggest that AFD have an anticaries effect similar to that of regular FD.

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