In situ evaluation of 200 ppm fluoride toothpaste content trimetaphosphate, xylitol and erythritol on enamel demineralization and dental biofilm

ABSTRACT Aim This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization. Materials and methods During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SETM (self-etch, fluoride/MDPB free adhesive, AB) and Clearfil Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S (ƒ¢S) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 ƒÊm from the restoration margin. Data were analyzed by ANOVA. Results No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm. Conclusion All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research. How to cite this article de Vasconcelos SMLC, de Melo MAS, Wenceslau JPMS, Zanin ICJ, Beltrao HCP, Fernandes CAO, de Almeida PC, Rodrigues LKA. In situ Assessment of Effects of the Bromide- and Fluoride-Incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014;15(2):142-148.

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Effect of starch on the cariogenic potential of sucrose

British Journal Of Nutrition ◽

10.1079/bjn20051452 ◽

2005 ◽

Vol 94 (1) ◽

pp. 44-50 ◽

Cited By ~ 44

Author(s):

Cecilia C. C. Ribeiro ◽

Cínthia P. M. Tabchoury ◽

Altair A. Del Bel Cury ◽

Livia M. A. Tenuta ◽

Pedro L. Rosalen ◽

...

Keyword(s):

Animal Studies ◽

Amylase Activity ◽

Negative Control ◽

Cross Sectional ◽

Dental Biofilm ◽

Enamel Demineralization ◽

Two Phases ◽

Mineral Loss

Sincein vitroand animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessedin situthe effects of this association appliedin vitroon the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0·05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0·05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.

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Toothpaste with Nanosized Trimetaphosphate Reduces Enamel Demineralization

JDR Clinical & Translational Research ◽

10.1177/2380084416683913 ◽

2016 ◽

Vol 2 (3) ◽

pp. 233-240 ◽

Cited By ~ 5

Author(s):

M.D.B. Souza ◽

J.P. Pessan ◽

C.S. Lodi ◽

J.A.S. Souza ◽

E.R. Camargo ◽

...

Keyword(s):

Protective Effect ◽

Surface Hardness ◽

Extracellular Polysaccharide ◽

Degree Of Saturation ◽

Double Blind ◽

Cross Sectional ◽

Fluoride Toothpaste ◽

Sodium Trimetaphosphate ◽

Enamel Demineralization

This double-blind crossover study assessed the effects of a low-fluoride (low-F) dentifrice containing nanosized sodium trimetaphosphate (TMP) on enamel demineralization in situ. Nineteen subjects wore palatal appliances containing 4 blocks of bovine enamel and were randomly assigned to brush their teeth with placebo (without F/TMP), 250-ppm F (250F), 250F plus 0.05% nanosized TMP (250F-TMPnano), and 1,100-ppm F (1,100F) dentifrices during 7 d, under cariogenic challenge. Enamel surface hardness and cross-sectional hardness (ΔKHN [Knoop hardness number]), as well as F, calcium (Ca), and phosphorus (P) concentrations, were determined. Also, biofilm that formed on the blocks was analyzed for F, Ca, P, and insoluble extracellular polysaccharide concentrations. Data were submitted to analysis-of-variance models and Student-Newman-Keuls test ( P < 0.05). The 250F-TMPnano dentifrice promoted the lowest ΔKHN among all groups ( P < 0.001), while the percentage of surface hardness loss was similar to 1,100F. Also, similar F, Ca, and P concentrations in enamel were observed for 1,100F and 250F-TMPnano. In the biofilm, the highest F content was observed for 1,100F; Ca content was similar between 1,100F and 250F-TMPnano; and P content was similar among all groups. Similar extracellular polysaccharide values were observed for 250F-TMPnano and 1,100F ( P < 0.001), ionic activity of CaHPO40, CaF+, and HF0 ( P < 0.05) and degree of saturation of hydroxyapatite and CaF2 ( P < 0.05). It was concluded that the protective effect of 250F-TMPnano dentifrice was similar to a conventional dentifrice for most of the variables studied, having a more pronounced effect on the subsurface lesion when compared with the conventional toothpaste (1,100F). Knowledge Transfer Statement: Although toothpastes containing ≥1,000-ppm fluoride are more effective than low-fluoride formulations against dental caries, their early use can lead to side effects. This has prompted intensive research on alternatives to increase the anticaries effect of low-fluoride toothpastes. The present in situ study demonstrated that the addition of sodium trimetaphosphate nanoparticles to toothpastes containing 250-ppm fluoride significantly enhances the protective effect of this formulation against enamel demineralization to levels comparable to a 1,100-ppm fluoride toothpaste in terms of most of the variables studied. Most important, this formulation promoted the lowest loss of subsurface hardness among all groups, suggesting that caries lesions would take longer to develop under clinical conditions when compared with a conventional (1,100-fluoride) toothpaste.

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Effect of Sucrose Concentration on Dental Biofilm Formed in situ and on Enamel Demineralization

Caries Research ◽

10.1159/000088902 ◽

2005 ◽

Vol 40 (1) ◽

pp. 28-32 ◽

Cited By ~ 50

Author(s):

C.P. Aires ◽

C.P.M. Tabchoury ◽

A.A. Del Bel Cury ◽

H. Koo ◽

J.A. Cury

Keyword(s):

Sucrose Concentration ◽

Dental Biofilm ◽

Enamel Demineralization

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Effectiveness of a Toothpaste with Low Fluoride Content Combined with Trimetaphosphate on Dental Biofilm and Enamel Demineralization in situ

Caries Research ◽

10.1159/000381960 ◽

2015 ◽

Vol 49 (4) ◽

pp. 394-400 ◽

Cited By ~ 14

Author(s):

Eliana M. Takeshita ◽

Marcelle Danelon ◽

Luciene P. Castro ◽

Kikue T. Sassaki ◽

Alberto C.B. Delbem

Keyword(s):

Sucrose Solution ◽

Blind Study ◽

Double Blind Study ◽

Oral Appliances ◽

Double Blind ◽

Sodium Trimetaphosphate ◽

Dental Biofilm ◽

Enamel Demineralization ◽

Mineral Loss

Objective: The aim of the present study was to evaluate in situ whether a toothpaste with low fluoride associated with sodium trimetaphosphate (TMP) would provide similar effect to that of a 1,100 ppm F toothpaste. Design: This crossover double-blind study consisted of 4 phases (14 days each), during which 10 volunteers wore oral appliances containing 4 enamel bovine blocks. The cariogenic challenge was performed by the application of a 20% sucrose solution (6×/day). The toothpaste treatments (2×/day) were: placebo, 500 ppm F, 500 ppm F plus 1% TMP, and 1,100 ppm F. At the end, enamel mineral loss and biofilm composition were analyzed. Results: The toothpaste with 500 ppm F plus 1% TMP showed the lowest mineral loss (p < 0.05). Regarding the fluoride and calcium concentrations in the enamel and in the biofilm, there were no significant differences between 500 ppm F plus 1% TMP, and 1,100 ppm F toothpastes (p > 0.569), but they were significantly different when compared to toothpaste with 500 ppm F (p < 0.050). Conclusion: The addition of 1% TMP to a low-fluoride toothpaste reduces enamel demineralization in situ similar to a 1,100 ppm F toothpaste.

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Effect of Sucrose Containing Iron (II) on Dental Biofilm and Enamel Demineralization in situ

Caries Research ◽

10.1159/000083157 ◽

2005 ◽

Vol 39 (2) ◽

pp. 123-129 ◽

Cited By ~ 35

Author(s):

G.D. Pecharki ◽

J.A. Cury ◽

A.F. Paes Leme ◽

C.P.M. Tabchoury ◽

A.A. Del Bel Cury ◽

...

Keyword(s):

Dental Biofilm ◽

Enamel Demineralization

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Effectiveness of a toothpaste with low fluoride content combined with trimetaphosphate on dental biofilm and enamel demineralization in situ

BDJ ◽

10.1038/sj.bdj.2016.174 ◽

2016 ◽

Vol 220 (5) ◽

pp. 239-239

Keyword(s):

Fluoride Content ◽

Dental Biofilm ◽

Enamel Demineralization

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Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure

Brazilian Dental Journal ◽

10.1590/s0103-64402007000300002 ◽

2007 ◽

Vol 18 (3) ◽

pp. 185-191 ◽

Cited By ~ 6

Author(s):

Rodrigo Alex Arthur ◽

Cínthia Pereira Machado Tabchoury ◽

Renata de Oliveira Mattos-Graner ◽

Altair A. Del Bel Cury ◽

Adriana Franco Paes Leme ◽

...

Keyword(s):

Genotypic Diversity ◽

Negative Control ◽

Stress Exposure ◽

Chain Reaction ◽

Dental Biofilm ◽

Polymerase Chain ◽

Fermentable Carbohydrates ◽

Almost All ◽

Frequent Exposure

In situ dental biofilm composition under sugar exposure is well known, but sugar effect on the genotypic diversity of S. mutans in dental biofilm has not been explored. This study evaluated S. mutans genotypic diversity in dental biofilm formed in situ under frequent exposure to sucrose and its monosaccharide constituents (glucose and fructose). Saliva of 7 volunteers was collected for isolation of S. mutans and the same volunteers wore intraoral palatal appliances, containing enamel slabs, which were submitted to the following treatments: distilled and deionized water (negative control), 10% glucose + 10% fructose (fermentable carbohydrates) solution or 20% sucrose (fermentable and EPS inductor) solution, 8x/day. After 3, 7 and 14 days, the biofilms were colleted and S. mutans colonies were isolated. Arbitrarily primed polymerase chain reaction (AP-PCR) of S. mutans showed that salivary genotypes were also detected in almost all biofilm samples, independently of the treatment, and seemed to reflect those genotypes present at higher proportion in biofilms. In addition to the salivary genotypes, others were found in biofilms but in lower proportions and were distinct among treatment. The data suggest that the in situ model seems to be useful to evaluate genotypic diversity of S. mutans, but, under the tested conditions, it was not possible to clearly show that specific genotypes were selected in the biofilm due to the stress induced by sucrose metabolism or simple fermentation of its monosaccharides.

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