In vitro Synergistic Activity of 5-Fluorouracil with Low-Dose Ozone against a Chemoresistant Tumor Cell Line and Fresh Human Tumor Cells

Chemotherapy ◽  
1990 ◽  
Vol 36 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Kurt S. Zänker ◽  
Ronald Kroczek
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cells ◽  
Low Dose ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Synergistic Activity ◽  
Human Tumor Cells

scholarly journals Ultrastructure of double minutes from a human tumor cell line.

1979 ◽  
Vol 83 (3) ◽  
pp. 663-666 ◽  
Author(s):  
P E Barker ◽  
E Stubblefield
Keyword(s):  
Electron Microscopy ◽  
Chemical Analysis ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cells ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Tumor Cells ◽  
Human Tumor Cell ◽  
Double Minutes

Double minutes (dm) have been isolated from human tumor cells by zonal centrifugation and by differential pelleting of chromosome suspsension. These methods allowed collection of dm in sufficient quantity and purity for visualization with electron microscopy. Ultrastructurally, the chromatin fibers in dm resemble thrance fragments was found. When the two isolation protocols were compared, differential pelleting was shown to increase purity twofold to 85% dm by mass. The differential pelleting procedure enables easy collection of dm in sufficient quantity and purity for chemical analysis.

5-Aminolevulinic acid-based photodynamic therapy of chordoma: In vitro experiments on a human tumor cell line

2017 ◽  
Vol 20 ◽  
pp. 111-115 ◽  
Author(s):  
Jan F. Cornelius ◽  
Lennert Eismann ◽  
Lara Ebbert ◽  
Brigitte Senger ◽  
Athanasios K. Petridis ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Cell ◽  
Cell Line ◽  
Aminolevulinic Acid ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Tumor Cell Line ◽  
In Vitro Experiments ◽  
Human Tumor Cell

Positive And Negative Platelet Aggregation Responses To Human Tumor Cells Among Different Normal Individuals

1981 ◽  
Author(s):  
Antonio Ordinas ◽  
Eva Bastida ◽  
G A Jamieson
Keyword(s):  
Platelet Aggregation ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cells ◽  
Lung Carcinoma ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Cell Lung Carcinoma ◽  
Aggregation Response ◽  
Plasma Factor

Heparinized platelet-rich plasma from 16 normal donors has been examined for platelet aggregation in response to 6 cultured human tumor cell lines. All donors showed maximal aggregation equal to 10 μM ADP with U87MG (glioblastoma) with the exception of one donor (#7) who gave 50% response: donor 7 reacted with no other tumor cell line. With Hut 20 (large cell lung carcinoma, 105 cells/ml) six donors (#3, 4, 7, 14, 15 and 16) failed to show aggregation. With HT 29 (adenocarcinoma, 106 cells/ml), the same six donors (#3, 4, 7, 14, 15 and 16) plus three additional donors (#5, 9 and 10) failed to show aggregation with Hut 28 (mesothelioma) and SKNMC (neuroblastoma), the same nine donors (#3, 4, 5, 7, 9, 10, 14, 15 and 16) failed to show platelet aggregation at 5 × 106 cells/ml. None of the donors showed aggregation with the A549 line (epithelial lung carcinoma). Gel-filtered platelets show no aggregation response with any cell line. Crossover studies with responders and non-responders showed no differences in the platelets from different donors but did show that the aggregation response is dependent upon a plasma component. Interaction of platelets and tumor cells is thought to be an important factor in the metastatic dissemination of human cancer. Similar tumors are known to show different degrees of metastatic activity in different patients. The present results suggest that responders and non-respondiers may be identified from aggregation data and that this differentiation may depend on the presence of unidentified plasma factor(s).

scholarly journals Synthesis of Novel Methyl 7-[(Hetero)arylamino]thieno[2,3-b]pyrazine-6-carboxylates and Antitumor Activity Evaluation: Effects in Human Tumor Cells Growth, Cell Cycle Analysis, Apoptosis and Toxicity in Non-Tumor Cells

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4823
Author(s):  
Juliana M. Rodrigues ◽  
Ricardo C. Calhelha ◽  
António Nogueira ◽  
Isabel C. F. R. Ferreira ◽  
Lillian Barros ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cells ◽  
Cell Lines ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
African Green Monkey Kidney ◽  
Cell Lung Carcinoma ◽  
Cell Cycle Profile

Several novel methyl 7-[(hetero)arylamino]thieno[2,3-b]pyrazine-6-carboxylates were synthesized by Pd-catalyzed C–N Buchwald–Hartwig cross-coupling of either methyl 7-aminothieno[3,2-b]pyrazine-6-carboxylate with (hetero)arylhalides or 7-bromothieno[2,3-b]pyrazine-6-carboxylate with (hetero)arylamines in good-to-excellent yields (50% quantitative yield), using different reaction conditions, namely ligands and solvents, due to the different electronic character of the substrates. The antitumoral potential of these compounds was evaluated in four human tumor cell lines: gastric adenocarcinoma (AGS), colorectal adenocarcinoma (CaCo-2), breast carcinoma (MCF7), and non-small-cell lung carcinoma (NCI-H460) using the SRB assay, and it was possible to establish some structure–activity relationships. Furthermore, they did not show relevant toxicity against a non-tumor cell line culture from the African green monkey kidney (Vero). The most promising compounds (GI50 ≤ 11 µM), showed some selectivity either against AGS or CaCo-2 cell lines without toxicity at their GI50 values. The effects of the methoxylated compounds 2b (2-OMeC6H4), 2f and 2g (3,4- or 3,5-diOMeC6H3, respectively) on the cell cycle profile and induction of apoptosis were further studied in the AGS cell line. Nevertheless, even for the most active (GI50 = 7.8 µM) and selective compound (2g) against this cell line, it was observed that a huge number of dead cells gave rise to an atypical distribution on the cell cycle profile and that these cells were not apoptotic, which points to a different mechanism of action for the AGS cell growth inhibition.

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