Pigment Epithelium-Derived Factor Affects Angiogenesis in Celiac Disease

Objective: Recent studies have demonstrated that angiogenesis is impaired in patients with celiac disease (CD). In this study, we evaluated the levels of the novel antiangiogenic factor pigment epithelium-derived factor (PEDF) in CD patients. Methods: Eighty-four patients were included in the study; 71 patients with CD and 13 healthy controls. In the CD patient cohort, there were 21 newly diagnosed patients, 19 with adherence to a gluten-free diet and 31 practicing no adherence to this diet. The PEDF levels were measured using enzyme-linked immunosorbent assays. Results: The data revealed that celiac patients had higher levels of PEDF than did healthy controls. PEDF levels were not significantly different among the three CD groups. Additionally, the PEDF levels were not correlated with tissue transglutaminase IgA or IgG. Conclusions: Our data indicate that PEDF levels are significantly higher in CD patients than those in the healthy controls. This result suggests that PEDF negatively affects angiogenesis in CD. Although we did not observe any differences of PEDF levels among celiac patients, additional studies including more patients could clarify this issue.

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Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet

Nutrients ◽

10.3390/nu13072243 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2243

Author(s):

Katherine L. Olshan ◽

Ali R. Zomorrodi ◽

Meritxell Pujolassos ◽

Jacopo Troisi ◽

Nayeim Khan ◽

...

Keyword(s):

Celiac Disease ◽

Breast Milk ◽

Milk Composition ◽

Gluten Free Diet ◽

Intestinal Microbiome ◽

Healthy Controls ◽

Gluten Free ◽

Bacterial Strains ◽

Shotgun Metagenomics ◽

Functional Pathways

The intestinal microbiome may trigger celiac disease (CD) in individuals with a genetic disposition when exposed to dietary gluten. Research demonstrates that nutrition during infancy is crucial to the intestinal microbiome engraftment. Very few studies to date have focused on the breast milk composition of subjects with a history of CD on a gluten-free diet. Here, we utilize a multi-omics approach with shotgun metagenomics to analyze the breast milk microbiome integrated with metabolome profiling of 36 subjects, 20 with CD on a gluten-free diet and 16 healthy controls. These analyses identified significant differences in bacterial and viral species/strains and functional pathways but no difference in metabolite abundance. Specifically, three bacterial strains with increased abundance were identified in subjects with CD on a gluten-free diet of which one (Rothia mucilaginosa) has been previously linked to autoimmune conditions. We also identified five pathways with increased abundance in subjects with CD on a gluten-free diet. We additionally found four bacterial and two viral species/strains with increased abundance in healthy controls. Overall, the differences observed in bacterial and viral species/strains and in functional pathways observed in our analysis may influence microbiome engraftment in neonates, which may impact their future clinical outcomes.

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The Effect of Gluten-free Diet on Cardiovascular Risk Factors in Newly Diagnosed Pediatric Celiac Disease Patients

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/mpg.0000000000002235 ◽

2019 ◽

Vol 68 (5) ◽

pp. 684-688 ◽

Cited By ~ 3

Author(s):

Eyal Zifman ◽

Orith Waisbourd-Zinman ◽

Luba Marderfeld ◽

Noam Zevit ◽

Anat Guz-Mark ◽

...

Keyword(s):

Risk Factors ◽

Celiac Disease ◽

Cardiovascular Risk ◽

Cardiovascular Risk Factors ◽

Gluten Free Diet ◽

Gluten Free ◽

Newly Diagnosed

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A174 ASSESSMENT OF DISTRIBUTED EDUCATION ON GLUTEN-FREE DIET TO FAMILIES OF CHILDREN WITH NEWLY DIAGNOSED CELIAC DISEASE

Journal of the Canadian Association of Gastroenterology ◽

10.1093/jcag/gwy009.174 ◽

2018 ◽

Vol 1 (suppl_2) ◽

pp. 258-258

Author(s):

M Rashid ◽

J Haskett ◽

L Parkinson-McGraw ◽

A Noble ◽

J Van Limbergen ◽

...

Keyword(s):

Celiac Disease ◽

Gluten Free Diet ◽

Gluten Free ◽

Newly Diagnosed

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A Single Institution’s Experience of Primary Headache in Children With Celiac Disease

Journal of Child Neurology ◽

10.1177/0883073819873751 ◽

2019 ◽

Vol 35 (1) ◽

pp. 37-41 ◽

Cited By ~ 1

Author(s):

Grant L. Hom ◽

Brian L. Hom ◽

Barbara Kaplan ◽

A. David Rothner

Keyword(s):

Celiac Disease ◽

Tissue Transglutaminase ◽

Primary Headache ◽

Tension Type Headache ◽

Gluten Free Diet ◽

Gluten Free ◽

Phone Survey ◽

Type Headache ◽

The Impact ◽

Histologic Changes

Background: Few studies exist examining the frequency of primary headache in children with celiac disease and the impact of a gluten-free diet on primary headache symptomology. This study explores characteristics and frequency of headaches in children with celiac disease and response to gluten-free diet at a single institution. Methods: Medical records were reviewed for children with celiac disease confirmed by the presence of elevated tissue transglutaminase IgA levels and histologic changes consistent with the diagnosis of celiac disease on small bowel biopsy. Eligible participants were contacted via letter for participation in a phone survey regarding headaches. Phone interviews were conducted 2 weeks after notification and lasted approximately 10 minutes. Headaches were classified according to ICHD-3 criteria. Results: 247 eligible patients or their families were contacted. A total of 132 (53.44%) agreed to participate. One participant was excluded due to insufficient information provided. Overall, 51 of 131 participants had recurrent headache defined as at least 1 episode per month (39%, 95% confidence interval [CI]: 31%-47%) and 33 had migraine with or without aura (25%, 95% CI: 18%-33%). Twenty-eight had frequent tension-type headache (22%, 95% CI: 15%-29%). Thirty-two participants noted headaches before a confirmed diagnosis of celiac disease. Twenty-two of 32 participants (68.75%) noticed decreased headache frequency or intensity, or both, after starting the gluten-free diet. Conclusion: This study suggests that at least one-third of children and adolescents with celiac disease have recurrent headaches at the time of diagnosis. A gluten-free diet led to improved headache symptomology in a significant number of these patients.

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Thymic stromal lymphopoietin levels are increased in patients with celiac disease

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2019.4016 ◽

2019 ◽

Author(s):

Evrim Kahramanoğlu Aksoy ◽

Muhammet Yener Akpınar ◽

Ferdane Pirinççi Sapmaz ◽

Özlem Doğan ◽

Metin Uzman ◽

...

Keyword(s):

Celiac Disease ◽

Disease Duration ◽

Demographic Characteristics ◽

Thymic Stromal Lymphopoietin ◽

Healthy Controls ◽

Gluten Free ◽

Newly Diagnosed ◽

Median Serum ◽

Significant Difference ◽

Gastroenterology Outpatient Clinic

Thymic stromal lymphopoietin (TSLP) is a cytokine produced by epithelial cells in the lungs, skin and intestinal mucosa, and is involved in several physiological and pathological processes. In this study, we evaluated serum TSLP levels in patients with celiac disease (CD). The prospective study was conducted at a gastroenterology outpatient clinic between March 2018 and August 2018. Eighty-nine participants aged between 18 and 75 years were classified into following groups: 22 patients with newly diagnosed CD; 20 patients with CD who were compliant with a gluten-free diet (GFD); 32 patients with CD who were not compliant with a GFD; and 15 healthy controls. Demographic characteristics, disease duration, and selected biochemical and hematologic parameters were recorded and compared between groups. Median serum TSLP levels were 1193.65 pg/mL (range: 480.1–1547.1) in newly diagnosed CD patients, 110.25 pg/mL (range: 60.3–216.7) in CD patients who were compliant with a GFD, 113.1 pg/mL (range: 76.3–303.4) in CD patients who were not compliant with a GFD, and 57 pg/mL (range: 49–67.8) in healthy controls. Overall, there was a significant difference in serum TSLP levels between groups (p = 0.001). Patients with newly diagnosed CD had the highest serum TSLP levels. There was no significant difference in serum TSLP levels between patients with CD who were or were not compliant with a GFD. TSLP appears to be involved in the pathogenesis of CD. Further studies are required to determine if the TSLP signaling pathway can be used in the treatment of CD.

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Physical development in children with celiac disease in St. Petersburg

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-188-4-116-123 ◽

2021 ◽

pp. 116-123

Author(s):

N. S. Shapovalova ◽

V. P. Novikova ◽

K. A. Klikunova

Keyword(s):

Body Weight ◽

Celiac Disease ◽

Gastrointestinal Disease ◽

Physical Development ◽

Gluten Free Diet ◽

Gluten Free ◽

Newly Diagnosed ◽

Normal Body ◽

Normal Body Weight ◽

Group 1

Aim: to compare physical development in children with celiac disease (CD), depending on adherence to a gluten-free diet (GFD), and in children without CD.Materials and methods: 176 children with gastrointestinal disease were examined. Group 1 consisted of 58 children with newly diagnosed CD and chronic gastritis (CG), before the appointment of a gluten-free diet (GFD), group 2–49 children with CD and CG, adhered of GFD for 1 year, the comparison group consisted of 69 children with CG and duodenitis and excluded CD. The assessment of anthropometric data was carried out using the WHO AnthroPlus software (2009), with the determination of BMI and Z-score values: WAZ, SD; WHZ, SD; HAZ, SD; BAZ, SD.Results: The groups of participants in the study were homogeneous in terms of gender and age. BMI (16,57±3,29; 1,96 ± 3,96; 18,17±,22; t1,2=0,573; t1,3=0,007; t2,3=0,009). Weight deficiency was more often observed in patients in group 1 (56,8%, 4,1%, 23,2%; P1,2=0,00; P1,3=0,00; P2,3=0,02). Obesity was detected in the groups equally (1,7%, 4,1%, 4,3%; P1,2=0,00; P1,3=0,01; P2,3=0,04). Normal body weight was most common in groups 2 (39,7%, 91,8%, 7205%; P1,2=0,00; P1,3=0,01; P2,3=0,04). HAZ, SD statistically differed in all groups (–0,3928±1,13; 0,0133±1,06; 0,4664±1,15; t1,2=0,008, t1,3=0,004, t2,3=0,031).Conclusion: normal body weight was most typical for children on GFD. HAZ, SD in children with CD increased in comparison with children with newly diagnosed CD, but it was less, than in group 3. After a one year of GFD, the improvement of weight has a more pronounced dynamics than growth.

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CELIAC DISEASE

The Professional Medical Journal ◽

10.29309/tpmj/2016.23.07.1643 ◽

2016 ◽

Vol 23 (07) ◽

pp. 807-811

Author(s):

Nagina Shahzadi ◽

Muhammad Almas Hashmi ◽

Sadida Bahawal

Keyword(s):

Celiac Disease ◽

Health Benefit ◽

Gluten Free Diet ◽

Control Group ◽

P Value ◽

Gluten Free ◽

Newly Diagnosed ◽

And Gender ◽

Disease Study ◽

Trial Setting

Objectives: Probiotics are alive organisms which confer health benefit whentaken at an appropriate dosage. This study was done to determine the efficacy of probiotics indecreasing the frequency of diarrhea in children with celiac disease. Study Design: Randomizedcontrolled trial. Setting: Department of Pediatrics/DHQ Allied Hospital, Faisalabad. Period:November 2011 to October 2012. Methods: Newly diagnosed patients with CD having diarrheawere included by consecutive non-probability sampling. Patients were randomized in twogroups using computer generated numbers. One group was given probiotic along with glutenfree diet while other was only prescribed gluten free diet. Reduction in frequency of diarrhea intwo groups was compared after a period of 28 days. Results: A total of 116 cases (58 in eachgroup) were enrolled. Mean age was 9.11 years. The groups were similar in age and gender ofpatients. Comparison of efficacy of probiotics in children with CD was done with control groupwhich revealed reduction in stools frequency to less than half in 86.21% (n=50) in probioticgroup but only 62.07% (n=36) in control group. P-value was 0.00015 which shows a highlysignificant difference in both groups. Conclusion: Probiotics in addition to gluten free diet arehighly effective in reducing the frequency of diarrhea in newly diagnosed patients with CD, incomparison with gluten free diet alone.

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Slow Decrease of Anti-Tissue Transglutaminase Antibody Positivity In Children With Celiac Disease After Starting the Gluten-Free Diet

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/mpg.0000000000002691 ◽

2020 ◽

Vol Publish Ahead of Print ◽

Author(s):

Chiara Monachesi ◽

Anil K. Verma ◽

Giulia Naspi Catassi ◽

Simona Gatti ◽

Elena Lionetti ◽

...

Keyword(s):

Celiac Disease ◽

Tissue Transglutaminase ◽

Gluten Free Diet ◽

Gluten Free ◽

Slow Decrease ◽

Antibody Positivity

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The Celiac Disease Patients’ Ability to Experience Pleasure

Gastroenterology Research and Practice ◽

10.1155/2019/2030751 ◽

2019 ◽

Vol 2019 ◽

pp. 1-5

Author(s):

Fabiana Zingone ◽

Monica Siniscalchi ◽

Luna Carpinelli ◽

Paola Iovino ◽

Letizia Zingone ◽

...

Keyword(s):

Celiac Disease ◽

Sexual Activity ◽

Hospital Staff ◽

Sexual Pleasure ◽

Gluten Free Diet ◽

Physical Contact ◽

Healthy Controls ◽

Gluten Free ◽

Dietary Compliance

The motivation or ability to experience pleasure has been scarcely studied in celiac disease (CeD). We aimed to investigate the hedonistic feelings/anhedonia and sexual pleasure in CeD patients on a gluten-free diet (GFD) compared to controls. We recruited adult CeD patients at follow-up consecutively visited from April 2017 to April 2018 and controls from the hospital staff and friends of CeD patients. Participants completed the Snaith–Hamilton Pleasure Scale, measuring the levels of anhedonia, and answered three questions about physical contact, sexual activity, and modification of their life on a GFD. We included 178 CeD patients and 173 healthy controls. Seventeen patients (9.5%) and fourteen controls (8.1%) had anhedonia. We did not find any correlation between the presence of anhedonia and the length in years of GFD neither with the dietary compliance and age at the test. 10.7% patients and 8.7% controls reported of not having pleasure in physical contact and 5.06% CeD and 3.5% controls in feeling attraction for another person; 36.56% said a worsening of their life on a GFD. Our results show that CeD patients on a GFD are similar to controls in anhedonia and sexual problems, despite one-third reported a worsening of their life.

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Gluten-related immunogenic peptides in stool as means to monitor compliance with gluten-free diet in children with newly dia gnosed coeliac disease

Gastroenterologie a hepatologie ◽

10.48095/ccgh2021519 ◽

2021 ◽

Vol 75 (6) ◽

pp. 519-523

Author(s):

Radim Vyhnánek ◽

Ziad Khaznadar ◽

Roman Vyhnánek ◽

Milan Paulík

Keyword(s):

Coeliac Disease ◽

Blood Sample ◽

Stool Sample ◽

Tissue Transglutaminase ◽

Sandwich Elisa ◽

Gluten Free Diet ◽

Gluten Free ◽

Newly Diagnosed ◽

Strict Adherence ◽

Immunogenic Peptides

Objectives and study: To compare the values of gluten-related immunogenic peptides (GIP) in stool and anti-tissue transglutaminase IgA antibodies (anti-tTG IgA) in blood in children newly diagnosed with coeliac disease (CD). Methods: All children (2–15 y) newly diagnosed with CD between May 2018 and May 2020 at our clinic who complied with the inclusion criteria were invited to join the prospective study. During workup for CD, a stool sample to measure GIP was taken together with a blood sample to measure anti-tTG IgA. All newly diagnosed children were invited 4 months later for a check-up. Children and their caregivers were asked about known non-compliance with the gluten-free diet (GFD), a blood sample was taken to measure the anti-tTG IgA, and a stool sample was collected to measure GIP. Blood was evaluated for anti-tTG IgA by ELISA, and the stool was tested by quantitative Sandwich ELISA designed to detect and quantify GIP using the G12 antibody. Values of GIP and anti-tTG IgA were compared in terms of their relation to the upper limit of normal (ULN) of the particular method. Results: 29 children (18 girls) were enrolled in the study. The values of GIP in stool at the time of diagnosis were above the ULN (0.15 µg/g) in all children. Average 4.21, median 3.29, standard deviation (SD) 3.7. After the four months, all but three (89.7%) had values of GIP in the reference range. Average 0.29, median 0.12, SD 0.73. Similarly, anti-tTG IgA values were above the ULN (9.9 U/mL) at the time of diagnosis in all children. Average 164, median 195, SD 49. Although the anti-tTG IgA levels were lower at check-up in all but one child, only 10 (34.5%) showed values within the normal range, with an average of 27.9, median 12.0, and SD 38.9. All children declared strict adherence to GFD. Discussion: Using the GIP concentration in stool, adherence to GFD in our cohort of children is very good, better than that described in literature. Conclusion: Measuring GIP in stool could prove a more sensitive indicator of adherence to GFD in the early months after the diagnosis of CD when anti-tTG IgA are still elevated above the ULN due to their well-described gradual decrease after GFD initiation.

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