Salidroside Attenuates Airway Inflammation and Remodeling via the miR-323-3p/SOCS5 Axis in Asthmatic Mice

2021 ◽  
pp. 1-11
Author(s):  
Xi Ming ◽  
Xingzhu Yu ◽  
Jijun Li ◽  
Junyu Wang ◽  
Jialin Zheng ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Inflammatory Cell ◽  
Quantitative Polymerase Chain Reaction ◽  
Resistance Index ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytokine Signaling

<b><i>Introduction:</i></b> Salidroside (Sal) a bioactive component extracted from <i>Rhodiola rosea</i> is remarkable for its anti-asthmatic effects. The study aimed to explore the molecular mechanism of Sal in airway inflammation and remodeling in asthmatic mice and provide a novel theoretical basis for asthma treatment. <b><i>Methods:</i></b> An asthmatic mouse model was established via ovalbumin (OVA) treatment, followed by injection of Sal and transfection of miR-323-3p-mimic and sh- suppressor of cytokine signaling 5 (SOCS5). Expressions of miR-323-3p, SOCS5 mRNA, collagen (COL)-I, and COL-III were detected via reverse transcription quantitative polymerase chain reaction. SOCS5 protein level was detected via Western blot. Levels of IgE, IL-13, IL-4, and IL-5 were detected via enzyme-linked immunosorbent assay. Inflammatory cell infiltration was observed via hematoxylin-eosin staining. Collagen disposition was observed via Masson staining. Resistance index (RI) of airway hyperresponsiveness, and the number of total cells, inflammatory cells (eosinophil, macrophage, neutrophil, and lymphocyte) in bronchoalveolar lavage fluid (BALF) were observed. The binding relationship between miR-323-3p and SOCS5 was predicted through the RNA22 website and verified via dual-luciferase reporter assay. <b><i>Results:</i></b> miR-323-3p was highly expressed in OVA-treated mice. Sal treatment reduced inflammatory cell infiltration, COL disposition, miR-323-3p expression, and IgE, IL-13, IL-4, IL-5, COL-I, and COL-III levels, RI value, and the number of total cells and inflammatory cells in BALF. miR-323-3p inhibited SOCS5 transcription. miR-323-3p overexpression or SOCS5 downregulation reversed the protecting role of Sal in asthmatic mice. <b><i>Conclusion:</i></b> Sal inhibited miR-323-3p expression to promote SOCS5 transcription, thereby attenuating airway inflammation and remodeling in asthmatic mice.

scholarly journals 16. Healing Process Of Burns (Vulnus combustion) Degrees IIB Using Mixed Leaf (Spondias dulcis F.) Fresh And Dry With Vaselin In Rats (Rattus Norvegicus)

2019 ◽  
Vol 13 (1) ◽  
pp. 114-124
Author(s):  
Ummu Balqis ◽  
Mirna Safrani Fauzi ◽  
Zuhrawati NA ◽  
Nazaruddin Nazaruddin ◽  
Razali Daud ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Inflammatory Cell ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Treatment Groups ◽  
White Rats ◽  
Grade Ii ◽  
Hematoxylin And Eosin

           The aims of this research was looked to determine the healing process of burns  Grade II B using a mixture of fresh and dried leaf kedondong with vaseline white rats (Rattus norvegicus). Animals used were 18 rats. This study was designed using three treatment groups namely (KI) vaseline, (KII) mixture of crushed leaves and fresh kedondong (KIII) mixture of crushed dried leaves kedondong and each two replications. Burns made on the backs of mice and the treatments are done twice a day for 21 days.Observation research parameter is the description of macroscopic and microscopic observed at day 7, 14, and 21 in the skin tissue with hematoxylin and eosin staining. The observation of macroscopic studies showed that the formation of a reddish color, presence of edema, and loss of the fastest consecutive scab is KI, KIII, and KII, while the formation of a scab fastest respectively KIII, KI and KII. The observation of histopathology showed on day 7, KI, KII and KIII found inflammatory cell infiltration, hemorrhage and edema with the spread of many.on the 14th day of KI and KII infiltration of inflammatory cells and their udema much, whereas KIII inflammatory cell infiltration decreased, and hyperemia increase, on the 21st day KI, KII and KII inflammatory cell infiltration, hyperemia and hemorrhage spread with a little, but KI and KII oedemanya still being spread. Based on the results of this study concluded that KIII accelerate the healing process of burns on rats

Humic Acid, a Polyphenolic Substance, Decreases Alveolar Bone Loss in Experimental Periodontitis in Rats

2019 ◽  
Vol 36 (4) ◽  
pp. 257-265
Author(s):  
Metin Çalışır ◽  
Aysun Akpınar ◽  
Ömer Poyraz ◽  
Fahrettin Göze ◽  
Ziynet Çınar
Keyword(s):  
Humic Acid ◽  
Bone Loss ◽  
Inflammatory Cell ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Local Administration ◽  
Experimental Periodontitis

The purpose of this study was to evaluate the biochemical, morphometric, and histopathological changes associated with experimental periodontitis in rats in response to local administration of humic acid. Thirty-eight Wistar rats were divided into 5 experimental groups: nonligated (NL) group, ligature-only (LO) group, and ligature + local administration of humic acid (20, 80, and 150 mg/kg body weight per day for 15 days, respectively; L-20, L-80, and L-150 groups). Changes in alveolar bone levels were clinically measured as the distance from the cementoenamel junction to the alveolar bone crest with a stereomicroscope. Tissues were histopathologically examined to assess the osteoclast numbers, osteoblastic activity, and inflammatory cell infiltration among the study groups. Enzyme-linked immunosorbent assay interleukin1β (IL-1β) and IL-10 levels in serum and gingival homogenates were evaluated. At the end of 15 days, the alveolar bone loss was significantly higher in the LO group compared to the NL, L-20, and L-150 groups ( P < .05). The osteoclast number in the LO group was significantly higher than the NL, L-20, and L-150 groups ( P < .05). Inflammatory cell infiltration was significantly higher in the LO and L-80 groups than the other groups ( P < .05). The highest serum and gingival homogenate IL-10 levels were determined in the NL group ( P < .05). The serum and gingival homogenate IL-1β levels in LO group were significantly higher than the NL, L-20, and L-150 groups ( P < .05). Within the limits of this study, it can be suggested that humic acid, when administered locally at 20 and 80 mg/kg doses, may prevent alveolar bone loss in the rat model.

scholarly journals Mucinous cystic adenoma with inflammatory cell infiltration around the splenic artery mimicking pancreatic cancer: a case report

2020 ◽  
Vol 13 (6) ◽  
pp. 1360-1366
Author(s):  
Hiroaki Okuse ◽  
Reiko Yamada ◽  
Kyosuke Tanaka ◽  
Noriyuki Horiki ◽  
Yoshiyuki Takei
Keyword(s):  
Distal Pancreatectomy ◽  
Splenic Artery ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Needle Aspiration ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Low Grade ◽  
Cystic Neoplasms ◽  
Cystic Adenoma

AbstractA 45-year-old woman presented with upper abdominal and back pain. A cystic lesion in the pancreas and inflammation around the splenic artery were detected by computed tomography. Although imaging studies were difficult to exclude malignancy, pathological and cytological findings of a fine-needle aspiration showed no signs of malignancy. The patient was, therefore, followed-up for 3 months, during which time the cyst increased in size and developed a cyst-in-cyst structure. She was diagnosed with mucinous cyst neoplasm and underwent distal pancreatectomy. Histologically, the patient was diagnosed as low-grade mucinous cystic adenoma. Soft tissue shadows around the splenic artery were considered to indicate fibrosis and infiltration of inflammatory cells. After distal pancreatectomy, the patient has been uneventful with symptom resolution. This case highlights the potentially atypical presentation of mucinous cystic neoplasms with inflammatory cell infiltration around the splenic artery.

Correlation of clinico-pathologic data with inflammatory cells infiltration in colorectal cancer

2020 ◽  
Vol 10 (1) ◽  
pp. 69-76
Author(s):  
M Grudzińska ◽  
K Jakubowska ◽  
L Kańczuga-Koda ◽  
W Kisielewski ◽  
W Famulski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumour Growth ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Histopathological Diagnosis ◽  
Tumour Mass ◽  
Invasive Front ◽  
Pathologic Features

Introduction: Colorectal cancer (CRC) is the third most common cancer worldwide. At every phase of cancer development, the inflammatory process has an important impact. Accurate assessment inflammatory cells in the tumour environment in conjunction with clinico-pathologic features can be a relevant prognostic or predictive parameter. Purpose: To analyse inflammatory cell infiltration in CRC tumour mass and correlate with chosen clinico-pathologic parameters. Materials and methods: The study group consisted of 160 patients (64 women, 96 men) diagnosed with colorectal cancer who underwent surgery. Tissue material obtained from routine histopathological diagnosis was stained with H&E and used to assess the type of inflammatory cells in the invasive front and centre of the tumour. Results were subjected to statistical analysis with the age and gender of patients, tumour localization, tumour growth and size, TNM stage, adenocarcinoma type, fibrosis, necrosis, metastasis and tumour invasion (by the Spearman’s correlation coefficient test). Results: The presence of neutrophils in the invasive front of tumour mass was associated with fibrosis and inflammatory cell infiltration in the invasive front of tumour. Macrophages in the invasive front of tumour were found to correlate with tumour growth (expanding and infiltrate). Macrophages and eosinophils were associated with inflammatory cell infiltration in the invasive front and in the centre of tumour. Conclusions: The type of inflammatory cells in the invasive front or centre of the tumour may be useful to prognoses clinical features of colorectal cancer

scholarly journals Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity

2001 ◽  
Vol 10 (3) ◽  
pp. 143-154 ◽  
Author(s):  
Fiona Westerhof ◽  
Wim Timens ◽  
Annemiek van Oosten ◽  
Annet B. Zuidhof ◽  
Nathalie Nauta ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inflammatory Cell ◽  
Allergen Challenge ◽  
Inflammatory Cells ◽  
Airway Hyperreactivity ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Airway Reactivity ◽  
Allergen Provocation ◽  
Time Point

Although airway inflammation and airway hyperreactivity are observed after allergen inhalation both in allergic humans and animals, little is known about the mechanisms by which inflammatory cells can contribute to allergen-induced airway hyperreactivity. To understand how inflammatory cell infiltration can contribute to airway hyperreactivity, the location of these cells within the airways may be crucial.Using a guinea pig model of acute allergic asthma, we investigated the inflammatory cell infiltration in different airway compartments at 6 and 24 h (i.e. after the early and the late asthmatic reaction, respectively) after allergen or saline challenge in relation to changes in airway reactivity (AR) to histamine.At 6 h after allergen challenge, a threefold(p<0.01)increase in the AR to histamine was observed. At 24 h after challenge, the AR to histamine was lower, but still significantly enhanced (1.6-fold,p<0.05).Adventitial eosinophil and neutrophil numbers in both bronchi and bronchioli were significantly increased at 6 h post-allergen provocation as compared with saline (p<0.01for all), while there was a strong tendency to enhanced eosinophils in the bronchial submucosa at this time point(p=0.08). At 24 h after allergen challenge, the eosinophilic and neutrophilic cell infiltration was reduced. CD3+T lymphocytes were increased in the adventitial compartment of the large airways(p<0.05)and in the parenchyma(p<0.05)at 24h post-allergen, while numbers of CD8+cells did not differ from saline treatment at any time point post-provocation.The results indicate that, after allergen provocation, inflammatory cell numbers in the airways are mainly elevated in the adventitial compartment. The adventitial inflammation could be important for the development of allergen-induced airway hyperreactivity.

scholarly journals Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jingru Wang ◽  
Shengnan Gao ◽  
Jingyuan Zhang ◽  
Chunxiao Li ◽  
Hongwen Li ◽  
...  
Keyword(s):  
Mouse Model ◽  
Airway Inflammation ◽  
Allergic Asthma ◽  
Inflammatory Cell ◽  
Allergic Airway Inflammation ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Interleukin 22 ◽  
Asthma Model ◽  
Asthma Mouse Model

Abstract Background Allergic asthma is a chronic airway inflammatory disease with a number of cytokines participating in its pathogenesis and progress. Interleukin (IL)-22, which is derived from lymphocytes, acts on epithelial cells and play a role in the chronic airway inflammation. However, the actual role of IL-22 in allergic asthma is still unclear. Therefore, we explored the effect of IL-22 on allergic airway inflammation and airway hyperresponsiveness (AHR) in an ovalbumin (OVA)-induced asthma mouse model. Methods To evaluate the effect of IL-22 in an allergic asthma model, BALB/c mice were sensitized and challenged with OVA; then the recombinant mouse IL-22 was administered intranasally 24 h prior to each challenge. The IL-22 levels in lung homogenates and bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay, respectively. AHR was evaluated through indicators including airways resistance (Rrs), elastance (Ers) and compliance (Crs); the inflammatory cell infiltration was assessed by quantification of differential cells counts in BALF and lung tissues stained by hematoxylin and eosin (H&E); IL-22 specific receptors were determined by immunohistochemistry staining. Results The concentration of IL-22 was significantly elevated in the OVA-induced mice compared with the control mice in lung homogenates and BALF. In the OVA-induced mouse model, IL-22 administration could significantly attenuate AHR, including Rrs, Ers and Crs, decrease the proportion of eosinophils in BALF and reduce inflammatory cell infiltration around bronchi and their concomitant vessels, compared with the OVA-induced group. In addition, the expression of IL-22RA1 and IL-10RB in the lung tissues of OVA-induced mice was significantly increased compared with the control mice, while it was dramatically decreased after the treatment with IL-22, but not completely attenuated in the IL-22-treated mice when compared with the control mice. Conclusion Interleukin-22 could play a protective role in an OVA-induced asthma model, by suppressing the inflammatory cell infiltration around bronchi and their concomitant vessels and airway hyperresponsiveness, which might associate with the expression of its heterodimer receptors. Thus, IL-22 administration might be an effective strategy to attenuate allergic airway inflammation.

A Case of Bullous Amyloidosis Associated with an Inflammatory Cell Infiltration and Prominent Pruritus

2008 ◽  
Vol 70 (3) ◽  
pp. 269-273
Author(s):  
Taisuke KAMIYAMA ◽  
Yoshihiro KAWAGUCHI ◽  
Masami SASAKI ◽  
Masamichi SATOU ◽  
Kumiko MIURA ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration
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