scholarly journals 16. Healing Process Of Burns (Vulnus combustion) Degrees IIB Using Mixed Leaf (Spondias dulcis F.) Fresh And Dry With Vaselin In Rats (Rattus Norvegicus)

2019 ◽  
Vol 13 (1) ◽  
pp. 114-124
Author(s):  
Ummu Balqis ◽  
Mirna Safrani Fauzi ◽  
Zuhrawati NA ◽  
Nazaruddin Nazaruddin ◽  
Razali Daud ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Inflammatory Cell ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Treatment Groups ◽  
White Rats ◽  
Grade Ii ◽  
Hematoxylin And Eosin

           The aims of this research was looked to determine the healing process of burns  Grade II B using a mixture of fresh and dried leaf kedondong with vaseline white rats (Rattus norvegicus). Animals used were 18 rats. This study was designed using three treatment groups namely (KI) vaseline, (KII) mixture of crushed leaves and fresh kedondong (KIII) mixture of crushed dried leaves kedondong and each two replications. Burns made on the backs of mice and the treatments are done twice a day for 21 days.Observation research parameter is the description of macroscopic and microscopic observed at day 7, 14, and 21 in the skin tissue with hematoxylin and eosin staining. The observation of macroscopic studies showed that the formation of a reddish color, presence of edema, and loss of the fastest consecutive scab is KI, KIII, and KII, while the formation of a scab fastest respectively KIII, KI and KII. The observation of histopathology showed on day 7, KI, KII and KIII found inflammatory cell infiltration, hemorrhage and edema with the spread of many.on the 14th day of KI and KII infiltration of inflammatory cells and their udema much, whereas KIII inflammatory cell infiltration decreased, and hyperemia increase, on the 21st day KI, KII and KII inflammatory cell infiltration, hyperemia and hemorrhage spread with a little, but KI and KII oedemanya still being spread. Based on the results of this study concluded that KIII accelerate the healing process of burns on rats

scholarly journals Mucinous cystic adenoma with inflammatory cell infiltration around the splenic artery mimicking pancreatic cancer: a case report

2020 ◽  
Vol 13 (6) ◽  
pp. 1360-1366
Author(s):  
Hiroaki Okuse ◽  
Reiko Yamada ◽  
Kyosuke Tanaka ◽  
Noriyuki Horiki ◽  
Yoshiyuki Takei
Keyword(s):  
Distal Pancreatectomy ◽  
Splenic Artery ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Needle Aspiration ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Low Grade ◽  
Cystic Neoplasms ◽  
Cystic Adenoma

AbstractA 45-year-old woman presented with upper abdominal and back pain. A cystic lesion in the pancreas and inflammation around the splenic artery were detected by computed tomography. Although imaging studies were difficult to exclude malignancy, pathological and cytological findings of a fine-needle aspiration showed no signs of malignancy. The patient was, therefore, followed-up for 3 months, during which time the cyst increased in size and developed a cyst-in-cyst structure. She was diagnosed with mucinous cyst neoplasm and underwent distal pancreatectomy. Histologically, the patient was diagnosed as low-grade mucinous cystic adenoma. Soft tissue shadows around the splenic artery were considered to indicate fibrosis and infiltration of inflammatory cells. After distal pancreatectomy, the patient has been uneventful with symptom resolution. This case highlights the potentially atypical presentation of mucinous cystic neoplasms with inflammatory cell infiltration around the splenic artery.

scholarly journals Healing Process Of Burns Using Ambarella Leaf (Spondias dulcis F.) and Vaselin in Rats (Rattus norvegicus)

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Ummu Balqis ◽  
Dian Masyitha ◽  
Fera Febrina
Keyword(s):  
Blood Vessels ◽  
Rattus Norvegicus ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Distilled Water ◽  
Inflammatory Reactions ◽  
Treatment Groups ◽  
Degree Burn ◽  
Speed Up ◽  
Collagen Tissue

The aims of this research was to find out the healing process of second degree burn in rats (Rattus norvegicus) using ambarella leaf (Spondias dulcis F.) and vaselin on histopathologically. The research consisted of 4 treatment groups and 3 rats each, P1(double distilled water), P2 (ambarella leaf + double distilled water), P3 (vaselin), and P4 (ambarella leaf + vaselin). Treatment was done twice a day for 21 days. The research parameters are inflammatory reactions and distribution of collagen tissue. Data were analyzed descriptively. The results showed that on day 7, P1 and P3 many infiltration of inflammatory cells, hemorrhage and edema, on P2 and P4 here more infiltration of inflammatory cells, hemorrhage and edema. On the day 14th of many inflammatory cell infiltration, collagen tissue diffuse very thin on were described P1 and P3, and visible presence of new blood vessels was found on P4. On day 21th infiltration of inflammatory cells still present on (P1), there were a lot of new blood vessels on P2, there has been a process of epithelialization (P3), epidermis formed and collagen tissue spread very densely on P4. Based on the results of this study it can be concluded that administration of ambarella leaf and vaselin can speed up the healing process of burns in rats compared with other treatments.Key words: ambarella leaf, burn, vaselin

scholarly journals PENGARUH BALUTAN BASAH OLIVE OIL TERHADAP PROSES PENYEMBUHAN LUKA BAKAR DERAJAT II PADA TIKUS PUTIH (RATTUS NORVEGICUS)

2021 ◽  
Vol 12 (2) ◽  
pp. 81-86
Author(s):  
Didit Damayanti
Keyword(s):  
Wound Healing ◽  
Olive Oil ◽  
Rattus Norvegicus ◽  
Healing Process ◽  
Control Design ◽  
Wound Healing Process ◽  
P Value ◽  
White Rats ◽  
Grade Ii ◽  
True Experiment

Luka bakar yang sering terjadi dan banyak ditemukan adalah luka bakar derajat II. Tumbuhan alam yang berkhasiat obat telah banyak dikenal oleh masyarakat Indonesia sejak lama. Salah satu tanaman alam yang mengandung banyak khasiat adalah olive oil. Olive oil memiliki kandungan senyawa penting dalam penyembuhkan luka antara lain oleochantal, sterol, fenol, squalen, vitamin C, E dan K yang dapat berperan mempercepat proses penyembuhan luka. Kandungan vitamin A dan vitamin E telah diketahui sebagai vitamin yang dapat membantu dalam proses penyembuhan luka. Penelitian ini menggunakan olive oil yang di buat dalam bentuk balutan basah untuk perawatan luka bakar derajat II pada tikus putih. Penelitian ini menggunakan metode true experiment dengan desain posttest only control design. Penelitian ini dilaksanakan pada bulan Maret 2020. Sampel penelitian adalah 20 kelompok perlakuan (tikus putih yang diberikan balutan basah olive oil) dan 20 kelompok kontrol tikus putih. Dengan teknik random sampling. Data diolah dengan SPSS menggunakan uji Mann Whitney. Hasil penelitian di dapatka P-value 0,00 < α  (0,05) yang berarti adanya pengaruh Perawatan Luka Bakar Derajat II Dengan Balutan Basah Olive Oil Pada Tikus Putih (Rattus Norvegicus). Diharapkan dengan pemanfaatan bahan alam untuk kepentingan sehari-hari dapat meminimalkan efek samping dari penggunaan bahan kimia yang sering digunakan oleh masyarakat saat ini. Kata Kunci: Luka Bakar Derajat II, Olive Oil, Tikus Putih (Rattus Norvegicus) Abstract Burns that often occur and are commonly found are second-degree burns. Natural medicinal plants have been widely known by the people of Indonesia for a long time. One of the natural plants that contain many benefits is olive oil. Olive oil has important compounds in wound healing including oleochantal, sterol, phenol, squalen, vitamins C, E and K which can play a role in accelerating the wound healing process. The content of vitamin A and vitamin E has been known as a vitamin that can help in the wound healing process. This study used olive oil which is made in the form of a wet dressing for the treatment of second-degree burns in white rats. This study used the true experiment method with the posttest only control design. The research was conducted in March 2020. The study sample was 20 treatment groups (white rats given wet olive oil dressing) and 20 control groups of white rats. With random sampling technique. Data were processed with SPSS using the Mann Whitney test. The results of the study obtained a P-value of 0.00 <α (0.05), which means that there is an influence of Grade II Burn Treatment with Olive Oil Wet Wrap on White Rat (Rattus Norvegicus). It is expected that the use of natural materials for daily use can minimize the side effects of the use of chemicals that are often used by the community today.   Keywords: Grade II Burns, Olive Oil, White Rat (Rattus Norvegicus)  

Correlation of clinico-pathologic data with inflammatory cells infiltration in colorectal cancer

2020 ◽  
Vol 10 (1) ◽  
pp. 69-76
Author(s):  
M Grudzińska ◽  
K Jakubowska ◽  
L Kańczuga-Koda ◽  
W Kisielewski ◽  
W Famulski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumour Growth ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Histopathological Diagnosis ◽  
Tumour Mass ◽  
Invasive Front ◽  
Pathologic Features

Introduction: Colorectal cancer (CRC) is the third most common cancer worldwide. At every phase of cancer development, the inflammatory process has an important impact. Accurate assessment inflammatory cells in the tumour environment in conjunction with clinico-pathologic features can be a relevant prognostic or predictive parameter. Purpose: To analyse inflammatory cell infiltration in CRC tumour mass and correlate with chosen clinico-pathologic parameters. Materials and methods: The study group consisted of 160 patients (64 women, 96 men) diagnosed with colorectal cancer who underwent surgery. Tissue material obtained from routine histopathological diagnosis was stained with H&E and used to assess the type of inflammatory cells in the invasive front and centre of the tumour. Results were subjected to statistical analysis with the age and gender of patients, tumour localization, tumour growth and size, TNM stage, adenocarcinoma type, fibrosis, necrosis, metastasis and tumour invasion (by the Spearman’s correlation coefficient test). Results: The presence of neutrophils in the invasive front of tumour mass was associated with fibrosis and inflammatory cell infiltration in the invasive front of tumour. Macrophages in the invasive front of tumour were found to correlate with tumour growth (expanding and infiltrate). Macrophages and eosinophils were associated with inflammatory cell infiltration in the invasive front and in the centre of tumour. Conclusions: The type of inflammatory cells in the invasive front or centre of the tumour may be useful to prognoses clinical features of colorectal cancer

Salidroside Attenuates Airway Inflammation and Remodeling via the miR-323-3p/SOCS5 Axis in Asthmatic Mice

2021 ◽  
pp. 1-11
Author(s):  
Xi Ming ◽  
Xingzhu Yu ◽  
Jijun Li ◽  
Junyu Wang ◽  
Jialin Zheng ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Inflammatory Cell ◽  
Quantitative Polymerase Chain Reaction ◽  
Resistance Index ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytokine Signaling

<b><i>Introduction:</i></b> Salidroside (Sal) a bioactive component extracted from <i>Rhodiola rosea</i> is remarkable for its anti-asthmatic effects. The study aimed to explore the molecular mechanism of Sal in airway inflammation and remodeling in asthmatic mice and provide a novel theoretical basis for asthma treatment. <b><i>Methods:</i></b> An asthmatic mouse model was established via ovalbumin (OVA) treatment, followed by injection of Sal and transfection of miR-323-3p-mimic and sh- suppressor of cytokine signaling 5 (SOCS5). Expressions of miR-323-3p, SOCS5 mRNA, collagen (COL)-I, and COL-III were detected via reverse transcription quantitative polymerase chain reaction. SOCS5 protein level was detected via Western blot. Levels of IgE, IL-13, IL-4, and IL-5 were detected via enzyme-linked immunosorbent assay. Inflammatory cell infiltration was observed via hematoxylin-eosin staining. Collagen disposition was observed via Masson staining. Resistance index (RI) of airway hyperresponsiveness, and the number of total cells, inflammatory cells (eosinophil, macrophage, neutrophil, and lymphocyte) in bronchoalveolar lavage fluid (BALF) were observed. The binding relationship between miR-323-3p and SOCS5 was predicted through the RNA22 website and verified via dual-luciferase reporter assay. <b><i>Results:</i></b> miR-323-3p was highly expressed in OVA-treated mice. Sal treatment reduced inflammatory cell infiltration, COL disposition, miR-323-3p expression, and IgE, IL-13, IL-4, IL-5, COL-I, and COL-III levels, RI value, and the number of total cells and inflammatory cells in BALF. miR-323-3p inhibited SOCS5 transcription. miR-323-3p overexpression or SOCS5 downregulation reversed the protecting role of Sal in asthmatic mice. <b><i>Conclusion:</i></b> Sal inhibited miR-323-3p expression to promote SOCS5 transcription, thereby attenuating airway inflammation and remodeling in asthmatic mice.

The effect of OK-432 (Picibanil) injection on the histopathology of nasal turbinate

2015 ◽  
Vol 129 (12) ◽  
pp. 1208-1212
Author(s):  
S Sengul ◽  
İ Kaygusuz ◽  
M M Akin ◽  
Ş Yalcin ◽  
T Karlidag ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Cell Loss ◽  
Treated Group ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Saline Control ◽  
Histopathological Changes ◽  
Nasal Turbinate ◽  
Treatment Groups ◽  
Significant Difference

AbstractObjective:This study aimed to assess the histopathological effect of OK-432 (Picibanil) on rabbit nasal turbinates.Methods:A total of 21 rabbits were divided into 3 treatment groups and various parts of both nasal turbinates were injected with 0.5 ml OK-432, 0.2 ml OK-432 or 0.6 ml saline (control). Bilateral nasal turbinates were later excised and studied under light microscopy to assess any histopathological changes.Results:Animals in the 0.2 ml and 0.5 ml OK-432 groups exhibited mild ciliary loss, goblet cell loss and epithelial damage, and a marked increase in inflammatory cell infiltration, submucosal vascularisation and fibrosis. There was a significant difference in histopathological changes between the two OK-432 treated groups. In addition, each OK-432 treated group had significantly more inflammatory cell infiltration, increased submucosal vascularisation and fibrosis compared with controls.Conclusion:The marked fibrosis observed in OK-432-injected turbinates may be responsible for a reduction in turbinate size.

scholarly journals PERBANDINGAN PEMBERIAN MINYAK KUNING TELUR AYAM DAN BIOPLACENTON TERHADAP GAMBARAN HISTOLOGI KULIT

2021 ◽  
Vol 11 (1) ◽  
pp. 6-14
Author(s):  
Ratna Widyawati ◽  
Rondius Solfaine ◽  
Dian Ayu Kartikasari ◽  
Yoko Shagita
Keyword(s):  
Rattus Norvegicus ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Egg Yolk ◽  
Burn Wound ◽  
White Rats ◽  
Degree Burn ◽  
Comparable Effect ◽  
Chicken Egg Yolk ◽  
Second Degree

This study was conducted to determine the comparison of re-epithelialization, granulation and the number of inflammatory cells infiltration between topical application of chicken yolk oil and bioplacenton on white rat (Rattus norvegicus) skin that has induced with second degree burn wound. This research was a laboratory experimental study using 18 male white rats divided into control positive (K+) group, bioplacenton (P1) and chicken yolk oil (P2). Observation of the re-epithelialization, granulation and inflammatory cells infiltration were carried out 14 days after the rat were induced with second degree burn wound. The necropcy was performed on the 14th day and then the skin organs were treated with HE staining and observed under a microscope. Data analysis was performed using Kruskal-Wallis followed by Mann Whitney. The results of the analysis showed that K + had significantly different results (P <0.05) compared to P1 and P2 in the scores of re-epithelialization, granulation and the number of inflammatory cell infiltrations. P1 and P2 were not significantly different (P> 0.05). In this study, it can be concluded that chicken egg yolk has a comparable effect with bioplacenton toward the score of re-epithelialization, granulation and the number of inflammatory cell infiltration.

scholarly journals Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity

2001 ◽  
Vol 10 (3) ◽  
pp. 143-154 ◽  
Author(s):  
Fiona Westerhof ◽  
Wim Timens ◽  
Annemiek van Oosten ◽  
Annet B. Zuidhof ◽  
Nathalie Nauta ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inflammatory Cell ◽  
Allergen Challenge ◽  
Inflammatory Cells ◽  
Airway Hyperreactivity ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Airway Reactivity ◽  
Allergen Provocation ◽  
Time Point

Although airway inflammation and airway hyperreactivity are observed after allergen inhalation both in allergic humans and animals, little is known about the mechanisms by which inflammatory cells can contribute to allergen-induced airway hyperreactivity. To understand how inflammatory cell infiltration can contribute to airway hyperreactivity, the location of these cells within the airways may be crucial.Using a guinea pig model of acute allergic asthma, we investigated the inflammatory cell infiltration in different airway compartments at 6 and 24 h (i.e. after the early and the late asthmatic reaction, respectively) after allergen or saline challenge in relation to changes in airway reactivity (AR) to histamine.At 6 h after allergen challenge, a threefold(p<0.01)increase in the AR to histamine was observed. At 24 h after challenge, the AR to histamine was lower, but still significantly enhanced (1.6-fold,p<0.05).Adventitial eosinophil and neutrophil numbers in both bronchi and bronchioli were significantly increased at 6 h post-allergen provocation as compared with saline (p<0.01for all), while there was a strong tendency to enhanced eosinophils in the bronchial submucosa at this time point(p=0.08). At 24 h after allergen challenge, the eosinophilic and neutrophilic cell infiltration was reduced. CD3+T lymphocytes were increased in the adventitial compartment of the large airways(p<0.05)and in the parenchyma(p<0.05)at 24h post-allergen, while numbers of CD8+cells did not differ from saline treatment at any time point post-provocation.The results indicate that, after allergen provocation, inflammatory cell numbers in the airways are mainly elevated in the adventitial compartment. The adventitial inflammation could be important for the development of allergen-induced airway hyperreactivity.

scholarly journals Electro-Acupuncture Accelerate Pressure Ulcer Healing by Increasing Asic3 Expression in Mouse Model

2020 ◽  
Author(s):  
Yu-Jie Xie ◽  
Chi Zhang ◽  
Fu-Hua Sun ◽  
Wei Jiang ◽  
Sheng-min Guo ◽  
...  
Keyword(s):  
Pressure Ulcer ◽  
Inflammatory Cell ◽  
Healing Process ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Control Group ◽  
Group Model ◽  
Pathologic Change ◽  
Model Group ◽  
Rt Pcr

Abstract Background: The efficiency of insert electro-acupuncture stimulation (EAS) on improving pressure ulcer (PU) healing was still unclear. This study explored the effects of insert EAS on PU and provided the regulation information of acid-sensing ion channel 3 (Asic3) during EAS intervention.Methods: A total of 32 BALB/c mice were randomly divided into control group, model group, EAS group, ESA + APETx2 (Asic3 inhibitor) group, each for eight mice. The ulcer tissues of all mice were havest at 14 days after modeling. HE stains and mmunohistochemical staining evaluated the pathologic change, Western blot, and RT-PCR evaluated Asic3 expression. Results: Compared with the model group, EAS group showed alleviated epithelial thickness, increased number of fibroblasts, and reduced inflammatory cell infiltration. Thickened epithelium, decreased number of fibroblasts, and obvious inflammatory cell infiltration in the EAS+APETx2 group were no significant differences with the model group. Asic3 expression in PU tissues in the EAS group was elevated compared with the model group. In addition, Asic3 expression was decreased in EAS + APETx2 group than the EAS group. Conclusion: EAS can accelerate the PU healing process in mice, and EAS also could improve the expression of Asic3 in PU tissues.Trial-registration: Not applicaple.

scholarly journals Gambaran Makroskopis Persembuhan Luka Mukosa Buccal Tikus Putih (Rattus norvegicus) Pascapemberian Policresulen

2021 ◽  
Vol 4 (3) ◽  
pp. 57-58
Author(s):  
Tri Isyani Tunggadewi ◽  
Tony Wahyudi ◽  
Aisyah Alviatus Shofwan ◽  
Anggri Azhari Khoerunnisa ◽  
Huda Salahudin Darusman
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Healing Process ◽  
Mucosal Injury ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
White Rats ◽  
Biopsy Punch ◽  
Macroscopic Observation

This study aimed to investigate the effect of policresulen treatment with various concentrations on the wound healing in buccal mucosa of white rats (Rattus norvegicus) through macroscopically observation. The number of 54 rats were divided into six treatment groups policresulen with concentration of 36%, 10%, 5%, 1%, 0.3% and one control group. The buccal mucosal injury was made with a 0.5 cm biopsy punch. The treatment of mucosal injury was conducted by dropped policresulen on the 2nd day until the 5th day after the injury. Macroscopic observation under a stereo micro-scope was conducted on the wound diameter, redness, swelling, and necrose. The results showed that wound healing was influenced significantly with concentration of the policresulen. The best healing was shown on lowest concentration marked which has lowest size of the wound diameter (0.50 ± 0.00 cm), the lowest tissue redness (0.00 ± 0.00), lowest final swelling at a concentration of 0.3%. Clinical evaluation of wound tissue necrose parameters which were seen to be most severe at 10% policresulen administration followed by 36% concentration and the least necrose shown on policresulen administration 0.3%. Therefore, based on macroscopic images with parameters of wound diameter, redness, swelling, and necrose suggested that policresulen used for wound healing process are at a concentration of 0.3%.

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