Evaluation of rhizobacterial colonisation and the ability to induce Globodera pallida hatch

Nematology ◽  
2015 ◽  
Vol 17 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Eoin P. Lettice ◽  
Peter W. Jones
Keyword(s):  
Sugar Beet ◽  
Dry Weight ◽  
Coarse Sand ◽  
Globodera Pallida ◽  
Cyst Nematodes ◽  
Beet Root ◽  
Shoot Dry Weight ◽  
Sugar Beet Root

Three bacterial isolates, SB13 (Acinetobacter sp.), SB14 (Arthrobacter sp.) and SB15 (Bacillus sp.), were previously isolated from the rhizosphere of sugar beet (Beta vulgaris ssp. vulgaris) plants and shown to increase hatch of potato cyst nematodes in vitro. In this study, the three isolates were assayed for rhizosphere competence. Each isolate was applied to seeds at each of four concentrations (105-108 CFU ml−1) and the inoculated seeds were planted in plastic microcosms containing coarse sand. All three isolates were shown to colonise the rhizosphere, although to differing degrees, with the higher inoculation densities providing significantly better colonisation. The isolates increased sugar beet root and shoot dry weight. Isolates SB14 and SB15 were analysed for their ability to induce in vivo hatch of Globodera pallida in non-sterile soil planted with sugar beet. After 4 and 6 weeks, both isolates had induced significantly greater percentage hatch compared to controls.

scholarly journals Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Alemayehu Dugassa ◽  
Tesfaye Alemu ◽  
Yitbarek Woldehawariat
Keyword(s):  
Faba Bean ◽  
Early Treatment ◽  
Dry Weight ◽  
Phenotypic Traits ◽  
Dual Culture ◽  
Shoot Dry Weight ◽  
Detached Leaves ◽  
Moderately Resistant

Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits. Results Pseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety. Conclusions The results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended.

Phytotoxicity of 2,4-D and 2,4-Dichlorophenol to Red Clover (Trifolium pratense)

Weed Science ◽  
1989 ◽  
Vol 37 (6) ◽  
pp. 825-829 ◽  
Author(s):  
S. G. Taylor ◽  
D. G. Shilling ◽  
K. H. Quesenberry ◽  
G. R. Chaudhry
Keyword(s):  
Trifolium Pratense ◽  
Callus Tissue ◽  
Red Clover ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Whole Plant ◽  
The Difference ◽  
Plant Level

Whole plant and tissue culture experiments were conducted to determine the difference in phytotoxicity of 2,4-D and its metabolite, 2,4-DCP, to red clover. At the whole plant level, the mean concentration of 2,4-DCP (10 mM) required to cause 50% growth inhibition (I50) of shoot dry weight was 24 times greater than for 2,4-D (0.42 mM). Using callus tissue, the I50value for 2,4-DCP (0.28 mM) was 22 times greater than for 2,4-D (0.013 mM) based on dry weights. The callus tissue was 36 and 32 times more sensitive to 2,4-DCP and 2,4-D than shoot tissue based on dry weights, respectively. These data indicate that 2,4-DCP was less phytotoxic than 2,4-D to red clover both in vitro and in vivo.

An in vitro sugar beet root maggot (Tetanops myopaeformis) feeding assay

2005 ◽  
Author(s):  
Snezana D. Ivic-Haymes ◽  
Mark Boetel ◽  
Larry G. Campbell ◽  
Robert Dregsetb ◽  
Ann C. Smigocki
Keyword(s):  
Sugar Beet ◽  
Feeding Assay ◽  
Beet Root ◽  
Tetanops Myopaeformis ◽  
Sugar Beet Root ◽  
Root Maggot

Efficacy of Purpureocillium lilacinum AUMC 10149 as biocontrol agent against root-knot nematode Meloidogyne incognita infecting tomato plant

2024 ◽  
Vol 84 ◽  
Author(s):  
G. S. Isaac ◽  
M. M. El-Deriny ◽  
R. G. Taha
Keyword(s):  
Meloidogyne Incognita ◽  
Dry Weight ◽  
Root Knot Nematode ◽  
Egg Hatching ◽  
Population Number ◽  
Tomato Crop ◽  
Purpureocillium Lilacinum ◽  
Shoot Dry Weight

Abstract Root-knot nematode Meloidogyne incognita is among the biotic factors which has greatly affected both the yield and the quality of the tomato crop. The egg parasitic nematode, Purpureocillium lilacinum (Pl) is considered as one of the most promising agents in controlling and overcoming this plant pathogen. The nematicidal effect of the native isolate Pl AUMC 10149 on second stage juvenile’s survival and egg hatching of M. incognita at different times of exposure was tested in vitro. The obtained data showed that Pl gave a maximum percentage of J2 mortality (97.6%) and egg hatching inhibition (79.8%) after 72 hours of exposure. The potentiality of Pl as well as Bio-Nematon to control M. incognita infecting tomato was conducted using different times of application in vivo. Nine treatments with five replicates were used for such bioagents compared with the nematicide Oxamyl. Each seedling was inoculated with 1000 J2s of nematode/pot and 10 mL of Pl (1x1010 CFU/mL) or Bio-Nematon spore suspension (1x108 CFU/mL) 10mL/pot. The results indicated that the most effective treatments in reducing nematode population, number of galls and egg masses of M. incognita in plant roots was performed with treatment by Pl pre-planting and post-infection with Pl (Rf 1.9) giving a significant enhancement in plant length (64.9%), fresh weight (72.52%) and shoot dry weight (163.41%) without negatively impacting environment. Therefore, the present study confirmed that using P. lilacinum AUMC 10149 can be used as a practical supplement to environmentally friendly disease management of root-knot nematodes in Egypt.

Computing Model for Vibratory Digging-Out of Sugar Beet Roots

2014 ◽  
Vol 1 (2) ◽  
pp. 52-60
Author(s):  
V. Bulgakov ◽  
V. Adamchuk ◽  
H. Kaletnyk
Keyword(s):  
Differential Equations ◽  
Sugar Beet ◽  
Vertical Plane ◽  
Forward Motion ◽  
Admissible Velocity ◽  
Beet Root ◽  
Optimum Speed ◽  
Analytical Research ◽  
Sugar Beet Root ◽  
The Given

The new design mathematical model of the sugar beet roots vibration digging-out process with the plowshare vibration digging working part has been created. In this case the sugar beet root is simulated as a solid body , while the plowshare vibration digging working part accomplishes fl uctuations in the longitudinal - vertical plane with the given amplitude and frequency in the process of work . The aim of the current research has been to determine the dependences between the design and kinematic parameters of the sugar beet roots vibra- tion digging-out technological process from soil , which provide the ir non-damage. Methods . For the aim ac- complishment, the methods of design mathematical models constructing based on the classical laws of me- chanics are applied. The solution of the obtained differential equations is accomplished with the PC involve- ment. Results . The differential equations of the sugar beet root’s motion in course of the vibration digging-out have been comprised . They allow to determine the admissible velocity of the vibration digging working part’s forward motion depending on the angular parameters of the latter. In the result of the computational simula- tion i.e., the solution of the obtained analytical dependence by PC, the graphic dependences of the admissible velocity of plowshare v ibration digging working part’s forward motion providing the extraction of the sugar beet root from soil without the breaking-off of its tail section have been determined. Conclusions . Due to the performed analytical research , it has been established that γ = 13 ... 16 ° , β = 20 ... 30 ° should be considered as the most reasonable values of γ and β angles of the vibration digging working part providing both its forward motion optimum speed and sugar beet root digging-out from the soil without damage . On the ground of the data obtained from the analytical rese arch, the new vibration digging working parts for the sugar beet roots have been designed; also the patents of Ukraine for the inventions have been obtained for them.

Notes on Behaviour of Pemphigus betae Doane (Homoptera: Aphididae) Infected with Entomophthora aphidis Hoffm.

1958 ◽  
Vol 90 (7) ◽  
pp. 439-440 ◽  
Author(s):  
A. M. Harper
Keyword(s):  
Sugar Beet ◽  
Acyrthosiphon Pisum ◽  
Soil Surface ◽  
First Record ◽  
Department Of Agriculture ◽  
Beet Root ◽  
Pemphigus Betae ◽  
Below Ground ◽  
Sugar Beet Root ◽  
Annapolis Valley

Normally the sugar-beet root aphid, Pemphigus betae Doane, lives and feeds on sugar-beet roots below ground during the summer and fall. However, in many beet fields between Lethbridge and Monarch, Alberta, in September, 1956, a large number of these aphids were found on the soil surface and on the crowns and leaves of the plants. Many of the aphids had crawled up the plants and, even after death, remained clinging to the leaves (Fig. 1). This clinging reaction seemed similar to that of grasshoppers infected with Entomophthora grylli Fresen. Mr. R. B. Baird, Entomology Laboratory, Canada Department of Agriculture, Belleville, Ontario, identified the organism causing the disease destroying these aphids as Entomophthora aphidis Hoffm. This is the first record of this disease killing subterranean aphids in Canada. The only other reports of E. aphidis on root aphids are those of Maxson (1916) in Colorado and Charles (1941) in California. In Canada, it has been previously reported as a factor in control of the pea aphid, Acyrthosiphon pisum (Harr.), in the Annapolis Valley, Nova Scotia (MacLeod, 1953).

The ability of rhizosphere bacteria isolated from nematode host and non-host plants to influence the hatch in vitro of the two potato cyst nematode species, Globodera rostochiensis and G. pallida

Nematology ◽  
2004 ◽  
Vol 6 (3) ◽  
pp. 375-387 ◽  
Author(s):  
N. Aileen Ryan ◽  
Peter Jones
Keyword(s):  
Sugar Beet ◽  
Host Plant ◽  
Host Plants ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Globodera Pallida ◽  
Bacterial Isolates ◽  
Bacillus Spp

AbstractSeventy bacteria, isolated from the rhizosphere of the potato cyst nematode (PCN) host plant, potato, were cultured in the presence and absence of potato root leachate (PRL) and the resultant culture filtrates were analysed for their ability to affect the hatch in vitro of the two PCN species. Of the isolates tested, nine had a significant effect on PCN hatch. Six affected Globodera pallida hatch and three affected G. rostochiensis hatch. Five of the isolates significantly increased hatch only when cultured in the presence of PRL. Three of the isolates decreased PCN hatch significantly in PRL. Only one isolate increased hatch significantly in the absence of PRL. No isolate affected the hatch of both species. Six of the nine isolates that significantly affected PCN hatch had been pre-selected by culturing on PRL. Bacterial isolates from PCN non-hosts (14 from wheat, 17 from sugar beet) were also tested for hatching activity. The principal effect of the hatch-active isolates from the PCN non-host plants was to increase PCN hatch in the presence of PRL. In contrast to the host bacteria results, the isolates from non-host plants affected only G. rostochiensis hatch (three wheat isolates and four sugar beet isolates significantly increased G. rostochiensis hatch); no such isolate affected G. pallida hatch significantly in the presence of PRL. Ten isolates (32%) from non-host plants had the ability to increase significantly the hatch of PCN in the absence of PRL (eight of these affected G. rostochiensis hatch and four affected G. pallida hatch), compared to only one bacterial isolate (1%) from a host plant. The majority of the isolates from non-hosts produced PCN species-specific effects, as with the bacteria isolated from potatoes, although two wheat isolates increased the hatch of both species significantly in the absence of PRL. Of 20 hatch-active bacterial isolates (from all three plants) identified, 70% were Bacillus spp. Other genera identified were Arthrobacter , Acinetobacter and Staphylococcus .

scholarly journals Control of Volunteer Glyphosate-Resistant Canola in Glyphosate-Resistant Sugar Beet

2015 ◽  
Vol 29 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Vipan Kumar ◽  
Prashant Jha
Keyword(s):  
Sugar Beet ◽  
Field Experiments ◽  
Agricultural Research ◽  
Effective Control ◽  
Additional Advantage ◽  
Leaf Stage ◽  
Beet Root ◽  
Control Volunteer ◽  
Sugar Beet Root ◽  
Herbicide Programs

Occurrence of glyphosate-resistant (GR) canola volunteers in GR sugar beet is a management concern for growers in the Northern Great Plains. Field experiments were conducted at the Southern Agricultural Research Center near Huntley, MT, in 2011 and 2012 to evaluate effective herbicide programs to control volunteer GR canola in GR sugar beet. Single POST application of triflusulfuron methyl alone at the two-leaf stage of sugar beet was more effective at 35 compared with 17.5 g ai ha−1. However, rate differences were not evident when triflusulfuron methyl was applied as a sequential POST (two-leaf followed by [fb] six-leaf stage of sugar beet) program (17.5 fb 17.5 or 35 fb 35 g ha−1). Volunteer GR canola plants in the sequential POST triflusulfuron methyl–containing treatments produced little biomass (11 to 15% of nontreated plots) but a significant amount of seeds (160 to 661 seeds m−2). Ethofumesate (4,200 g ai ha−1) PRE followed by sequential POST triflusulfuron methyl (17.5 or 35 g ha−1) provided effective control (94 to 98% at 30 d after treatment [DAT]), biomass reduction (97%), and seed prevention of volunteer GR canola. There was no additional advantage of adding either desmedipham + phenmedipham + ethofumesate premix (44.7 g ha−1) or ethofumesate (140 g ha−1) to the sequential POST triflusulfuron methyl–only treatments. The sequential POST ethofumesate-only (140 fb 140 g ha−1) treatment provided poor volunteer GR canola control at 30 DAT, and the noncontrolled plants produced 6,361 seeds m−2, which was comparable to the nontreated control (7,593 seeds m−2). Sequential POST triflusulfuron methyl–containing treatments reduced GR sugar beet root and sucrose yields to 18 and 20%, respectively. Consistent with GR canola control, sugar beet root and sucrose yields were highest (95 and 91% of hand-weeded plots, respectively) when the sequential POST triflusulfuron methyl–containing treatments were preceded by ethofumesate (4,200 g ha−1) PRE. Growers should utilize these effective herbicide programs to control volunteer GR canola in GR sugar beet. Because of high canola seed production potential, as evident from this research, control efforts should be aimed at preventing seed bank replenishment of the GR canola volunteers.

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