Frequencies in vibrating wood – Does cell organization matter?

IAWA Journal ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 124-142
Author(s):  
Oliver Dünisch
Keyword(s):  
Spatial Organization ◽  
Ring Width ◽  
Spatial Arrangement ◽  
Cell Types ◽  
Dominant Frequency ◽  
Fast Fourier Transformation ◽  
Frequency Spectra ◽  
Laser Sensors ◽  
Vibrating Plates ◽  
Dominant Frequencies

ABSTRACT The relationship between the spatial organization of different cell types, of the xylem rays, and of the tree rings and the frequencies in vibrating softwoods and hardwoods was studied under controlled conditions. In total, the frequencies in 1007 standardized vibrating plates from 16 softwoods and 74 hardwoods were analysed using high resolution laser sensors (accuracy ± 0.02 μm, sampling frequency 30 kHz) for vibration measurements. Overlapping frequencies within the frequency spectra were identified by means of Fast Fourier Transformation analysis. With regard to the number of distinct frequencies within the spectra, four different vibration types were identified: type 1–one dominant frequency within the frequency spectra; type 2-two dominant frequencies within the frequency spectra; type 3-three dominant frequencies within the frequency spectra; type 4-no dominant frequencies within the frequency spectra. The presence of distinct frequencies was correlated with a highly organized spatial arrangement of tracheids in softwoods, with a storied arrangement of the xylem rays in hardwoods, and with low variation in tree-ring width in both softwoods and hardwoods. The grid size for repetition in these xylem structures influenced the frequencies of the vibrating wood in absolute numbers. The results indicate that the analysis of the anatomical structure of the wood can contribute to the grading of timber for its vibration characteristics, which is of special interest for the selection of resonance wood for musical instruments.

Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice

2001 ◽  
Vol 114 (24) ◽  
pp. 4429-4434
Author(s):  
Silvia Garagna ◽  
Maurizio Zuccotti ◽  
Alan Thornhill ◽  
Raul Fernandez-Donoso ◽  
Soledad Berrios ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Spatial Organization ◽  
Nuclear Architecture ◽  
Spatial Arrangement ◽  
Cell Types ◽  
Condensed State ◽  
Germ Cell Differentiation ◽  
Y Chromosomes ◽  
Dual Colour ◽  
Pachytene Spermatocytes

The mammalian cell nucleus consists of numerous compartments involved in the regular unfolding of processes such as DNA replication and transcription, RNA maturation, protein synthesis and cell division. Knowledge is increasing of the relationships between high-order levels of chromatin organization and its spatial organization, and of how these relationships contribute to the various functions carried out in the nucleus. We have studied the spatial arrangement of mouse telocentric chromosomes 5, 11, 13, 15, 16 and 17, some of their metacentric Robertsonian derivatives, and X and Y chromosomes by whole chromosome painting in male germ (spermatogonia, pachytene spermatocytes and spermatids) and Sertoli cells of homozygous and heterozygous individuals. Using dual-colour fluorescence in situ hybridization we found that these chromosomes occupy specific nuclear territories in each cell type analysed. When chromosomes are present as Robertsonian metacentrics in the heterozygous state, that is, as Robertsonian metacentrics and their homologous telocentrics, differences in their nuclear positions are detectable: heterozygosity regularly produces a change in the nuclear position of one of the two homologous telocentrics in all the cell types studied. In the Robertsonian heterozygotes, the vast majority of the Sertoli cells show the sex chromosomes in a condensed state, whereas they appear decondensed in the Robertsonian homozygotes. As the Robertsonian heterozygosities we studied produce a chromosomally derived impairment of male germ-cell differentiation, we discuss the possibility that changes in chromosome spatial territories may alter some nuclear machinery (e.g., synapsis, differential gene expression) important for the correct unfolding of the meiotic process and for the proper functioning of Sertoli cells.

Immuocytochemical analysis of spatial organization of photoreceptors and amacrine and ganglion cells in the tiger salamander retina

2004 ◽  
Vol 21 (2) ◽  
pp. 157-166 ◽  
Author(s):  
JIAN ZHANG ◽  
ZHUO YANG ◽  
SAMUEL M. WU
Keyword(s):  
Ganglion Cell ◽  
Spatial Organization ◽  
Ganglion Cells ◽  
Electrophysiological Study ◽  
Outer Nuclear Layer ◽  
Amacrine Cells ◽  
Spatial Arrangement ◽  
Cell Types ◽  
Proximal Region ◽  
Dorsal Retina

In the present study, using double- or triple-label immunocytochemistry in conjunction with confocal microscopy, we aimed to examine the population and distribution of photoreceptors, GABAergic and glycinergic amacrine cells, and ganglion cells, which are basic but important parameters for studying the structure–function relationship of the salamander retina. We found that the outer nuclear layer (ONL) contained 82,019 ± 3203 photoreceptors, of which 52% were rods and 48% were cones. The density of photoreceptors peaked at ∼8000 cells/mm2 in the ventral and dropped to ∼4000 cells/mm2 in the dorsal retina. In addition, the rod/cone ratio was less than 1 in the central retina but larger than 1 in the periphery. Moreover, in the proximal region of the inner nuclear layer (INL3), the total number of cells was 50,576 ± 8400. GABAergic and glycinergic amacrine cells made up approximately 78% of all cells in this layer, including 43% GABAergic, 32% glycinergic, and 3% GABA/glycine colocalized amacrine cells. The density of these amacrine cells was ∼6500 cells/mm2 in the ventral and ∼3200 cells/mm2 in the dorsal area. The ratio of GABAergic to glycinergic amacrine cells was larger than 1. Furthermore, in the ganglion cell layer (GCL), among a total of 36,007 ± 2010 cells, ganglion cells accounted for 65.7 ± 1.5% of the total cells, whereas displaced GABAergic and glycinergic amacrine cells comprised about 4% of the cells in this layer. The ganglion cell density was ∼1800 cells/mm2 in the ventral and ∼600 cells/mm2 in the dorsal retina. Our data demonstrate that all three major cell types are not uniformly distributed across the salamander retina. Instead, they exhibit a higher density in the ventral than in the dorsal retina and their spatial arrangement is associated with the retinal topography. These findings provide a basic anatomical reference for the electrophysiological study of this species.

scholarly journals A spatial atlas of inhibitory cell types in mouse hippocampus

2018 ◽  
Author(s):  
Xiaoyan Qian ◽  
Kenneth D. Harris ◽  
Thomas Hauling ◽  
Dimitris Nicoloutsopoulos ◽  
Ana B. Muñoz-Manchado ◽  
...  
Keyword(s):  
Spatial Organization ◽  
Memory Function ◽  
Expression Patterns ◽  
Ground Truth ◽  
Spatial Arrangement ◽  
Cell Types ◽  
Cell System ◽  
Inhibitory Neurons ◽  
Hippocampal Area

Understanding the function of a tissue requires knowing the spatial organization of its constituent cell types. In the cerebral cortex, single-cell RNA sequencing (scRNA-seq) has revealed the genome-wide expression patterns that define its many, closely related cell types, but cannot reveal their spatial arrangement. Here we introduce probabilistic cell typing by in situ sequencing (pciSeq), an approach that leverages prior scRNA-seq classification to identify cell types using multiplexed in situ RNA detection. We applied this method to map the inhibitory neurons of hippocampal area CA1, a cell system critical for memory function, for which ground truth is available from extensive prior work identifying the laminar organization of subtly differing cell types. Our method confidently identified 16 interneuron classes, in a spatial arrangement closely matching ground truth. This method will allow identifying the spatial organization of fine cell types across the brain and other tissues.

scholarly journals BMP4 patterns Smad activity and generates stereotyped cell fate organisation in spinal organoids

2019 ◽  
Author(s):  
Nathalie Duval ◽  
Célia Vaslin ◽  
Tiago Barata ◽  
Stéphane Nédélec ◽  
Vanessa Ribes
Keyword(s):  
Cell Fate ◽  
Bone Morphogenetic Proteins ◽  
Neural Tube ◽  
Spatial Organization ◽  
Time Windows ◽  
Spatial Dynamics ◽  
Spatial Arrangement ◽  
Cell Types ◽  
Dorsal Neural Tube ◽  
Bmp Signalling

AbstractBone Morphogenetic Proteins (BMP) are secreted regulators of cell fate in several developing tissues. In the embryonic spinal cord, they control the emergence of the neural crest, roof plate and distinct subsets of dorsal interneurons. Although a gradient of BMP activity has been proposed to determine cell type identity in vivo, whether this is sufficient for pattern formation in vitro is unclear. Here, we demonstrate that exposure to BMP4 initiates distinct spatial dynamics of BMP signalling within the self-emerging epithelia of both mouse and human pluripotent stem cells derived spinal organoids. The pattern of BMP signalling results in the stereotyped spatial arrangement of dorsal neural tube cell types and concentration, timing and duration of BMP4 exposure modulate these patterns. Moreover, differences in the duration of competence time-windows between mouse and human account for the species specific tempo of neural differentiation. Together the study describes efficient methods to generate patterned subsets of dorsal interneurons in spinal organoids and supports the conclusion that graded BMP activity orchestrates the spatial organization of the dorsal neural tube cellular diversity in mouse and human.

Computational Micrograph Registration with Sieve Processes

1996 ◽  
Vol 54 ◽  
pp. 440-441
Author(s):  
P.J. Phillips ◽  
J. Huang ◽  
S. M. Dunn
Keyword(s):  
Planar Graph ◽  
Efficient Algorithm ◽  
Spatial Organization ◽  
Spatial Arrangement ◽  
Stereo Image ◽  
Image Model ◽  
Geometric Invariants ◽  
Point Set

In this paper we present an efficient algorithm for automatically finding the correspondence between pairs of stereo micrographs, the key step in forming a stereo image. The computation burden in this problem is solving for the optimal mapping and transformation between the two micrographs. In this paper, we present a sieve algorithm for efficiently estimating the transformation and correspondence.In a sieve algorithm, a sequence of stages gradually reduce the number of transformations and correspondences that need to be examined, i.e., the analogy of sieving through the set of mappings with gradually finer meshes until the answer is found. The set of sieves is derived from an image model, here a planar graph that encodes the spatial organization of the features. In the sieve algorithm, the graph represents the spatial arrangement of objects in the image. The algorithm for finding the correspondence restricts its attention to the graph, with the correspondence being found by a combination of graph matchings, point set matching and geometric invariants.

ЛАНДШАФТНАЯ ОРГАНИЗАЦИЯ БЕРЕГОВОЙ ГЕОСТРУКТУРЫ ОСТРОВА ШКОТА (ЗАЛИВ ПЕТРА ВЕЛИКОГО)

2019 ◽  
Author(s):  
K.S. Ganzei ◽  
V.V. Zharikov ◽  
N.F. Pshenichnikova ◽  
A.M. Lebedev ◽  
A.G. Kiselyova ◽  
...  
Keyword(s):  
Spatial Organization ◽  
Peter The Great Bay ◽  
Spatial Arrangement ◽  
Water Area ◽  
Marine Spatial Planning ◽  
Landscape Approach ◽  
Important Condition ◽  
Statistical Comparison ◽  
Peter The Great ◽  
Comprehensive Study

Важнейшим условием достижения устойчивого развития прибрежноморского природопользования в заливе Петра Великого системы является морское пространственное планирование. Основой для этого является информация о природных комплексах территории и акватории, полученная на основе ландшафтного подхода. Ключевым районом для изучения пространственной организации ландшафтов прибрежных геоструктур стала территория острова Шкота и его подводных склонов. Для наземных ландшафтов было описано 49 наблюдательных пунктов, 4 профиля были заложены для подводных ландшафтов описано 64 наблюдательных пункта, проложено 18 профилей. Выделено 22 вида ландшафтов, из них 16 наземных, 6 подводных. Берега острова сформированы преобладанием абразивноденудационного и абразивного типов. В результате всестороннего изучения показаны особенности пространственной организации воздушных и водных природных комплексов. Особенностью исследуемой территории является экспозиция дифференциации ландшафтов между юговосточной и северозападной частями острова, обусловленная муссонной природой климата. Результаты полевых и картографических работ послужили основой для выбора зон интенсивного, умеренного и ослабленного взаимодействия наземных и подводных ландшафтов. Пространственное расположение зон взаимодействия четко иллюстрируется значительными различиями экспозиции. Результаты статистического сравнения ландшафтов суши и мелководья, окружающего остров, на основе картометрических характеристик указывают на неоднородность геоструктуры острова, обусловленную, прежде всего, сочетанием ландшафтообразующих факторов. The most important condition for achieving sustainable development of coastalmarine environmental management in Peter the Great Bay is marine spatial planning. The basis for this is information about the natural complexes of the territory and water area, obtained based on the landscape approach. The main area for studying the spatial organization of landscapes of coastal geostructures was the territory of the island of Shkota and its underwater slopes. For terrestrial landscapes, 49 observation points were described, 4 profiles were laid 64 observation points were described for underwater landscapes, 18 profiles were laid. 22 species of landscapes have been identified, of which 16 are terrestrial, 6 are underwater. The shores of the island are formed by the predominance of abrasivedenudation and abrasive types. Because of a comprehensive study, features of the spatial organization of air and aquatic natural complexes are shown. A special feature of the study area is the exposure of the differentiation of landscapes between the southeastern and northwestern parts of the island, due to the monsoon nature of the climate. The results of field and cartographic works served as the basis for selecting areas of intense, moderate and weakened interaction of land and underwater landscapes. The spatial arrangement of the interaction zones is clearly illustrated by significant differences in exposure. The results of a statistical comparison of the land and shallow water landscapes surrounding the island, based on the cartometric characteristics, indicate the heterogeneity of the islands geostructure, primarily due to the combination of landscapeforming factors.

scholarly journals High-Speed Video Analysis of the Process Stability in Plasma Spraying

2021 ◽  
Author(s):  
K. Bobzin ◽  
M. Öte ◽  
M. A. Knoch ◽  
I. Alkhasli ◽  
H. Heinemann
Keyword(s):  
Plasma Spraying ◽  
High Speed ◽  
Plasma Jet ◽  
Plasma Jets ◽  
Time Dependent ◽  
Acquisition Time ◽  
Fast Fourier Transformation ◽  
Frequency Spectra ◽  
Dependent Signal ◽  
The Stability

AbstractIn plasma spraying, instabilities and fluctuations of the plasma jet have a significant influence on the particle in-flight temperatures and velocities, thus affecting the coating properties. This work introduces a new method to analyze the stability of plasma jets using high-speed videography. An approach is presented, which digitally examines the images to determine the size of the plasma jet core. By correlating this jet size with the acquisition time, a time-dependent signal of the plasma jet size is generated. In order to evaluate the stability of the plasma jet, this signal is analyzed by calculating its coefficient of variation cv. The method is validated by measuring the known difference in stability between a single-cathode and a cascaded multi-cathode plasma generator. For this purpose, a design of experiment, covering a variety of parameters, is conducted. To identify the cause of the plasma jet fluctuations, the frequency spectra are obtained and subsequently interpreted by means of the fast Fourier transformation. To quantify the significance of the fluctuations on the particle in-flight properties, a new single numerical parameter is introduced. This parameter is based on the fraction of the time-dependent signal of the plasma jet in the relevant frequency range.

scholarly journals Neev, a novel long non-coding RNA, is expressed in chaetoblasts during regeneration of Eisenia fetida

2019 ◽  
Author(s):  
Surendra Singh Patel ◽  
Sanyami Zunjarrao ◽  
Beena Pillai
Keyword(s):  
Eisenia Fetida ◽  
Spatial Organization ◽  
Cell Types ◽  
Ventral Side ◽  
Chitin Synthase ◽  
Chitin Synthesis ◽  
Mirna Sponge ◽  
Non Coding Rna ◽  
Temporal Expression Pattern ◽  
Long Non Coding Rna

AbstractEisenia fetida, the common vermicomposting earthworm, shows robust regeneration of posterior segments removed by amputation. During the period of regeneration, the newly formed tissue initially contains only undifferentiated cells but subsequently differentiates into a variety of cell types including muscle, nerve and vasculature. Transcriptomics analysis, reported previously, provided a number of candidate non-coding RNAs that were induced during regeneration. We found that one such long non-coding RNA (lncRNA) is expressed in the skin, only at the base of newly formed chaetae. The spatial organization and precise arrangement of the regenerating chaetae and the cells expressing the lncRNA on the ventral side clearly support a model wherein the regenerating tissue contains a zone of growth and cell division at the tip and a zone of differentiation at the site of amputation. The temporal expression pattern of the lncRNA, christened Neev, closely resembled the pattern of chitin synthase genes, implicated in chaetae formation. We found that the lncRNA harbours 49 sites for binding a set of four miRNAs while the Chitin Synthase 8 mRNA comprises 478 sites. The over-representation of shared miRNA sites suggests that lncRNA Neev may act as a miRNA sponge to transiently de-repress chitin synthase 8 during formation of new chaetae in the regenerating segments of Eisenia fetida.Summary statementThe earthworm, Eisenia fetida, regenerates posterior segments following amputation. The transcriptome of the regenerating worm revealed a novel lncRNA, expressed only at the base of regenerating chaetae. We propose that this lncRNA is a miRNA sponge that modulates chitin synthesis.

scholarly journals Three-dimensional electron microscopy of ribosomal chromatin in two higher plants: a cytochemical, immunocytochemical, and in situ hybridization approach.

1991 ◽  
Vol 39 (11) ◽  
pp. 1495-1506 ◽  
Author(s):  
P M Motte ◽  
R Loppes ◽  
M Menager ◽  
R Deltour
Keyword(s):  
Electron Microscopy ◽  
In Situ Hybridization ◽  
Spatial Organization ◽  
Higher Plants ◽  
Three Dimensional ◽  
Spatial Arrangement ◽  
Thin Sections ◽  
Cytoplasmic Dna ◽  
Immunocytochemical Techniques

We report the 3-D arrangement of DNA within the nucleolar subcomponents from two evolutionary distant higher plants, Zea mays and Sinapis alba. These species are particularly convenient to study the spatial organization of plant intranucleolar DNA, since their nucleoli have been previously reconstructed in 3-D from serial ultra-thin sections. We used the osmium ammine-B complex (a specific DNA stain) on thick sections of Lowicryl-embedded root fragments. Immunocytochemical techniques using anti-DNA antibodies and rDNA/rDNA in situ hybridization were also applied on ultra-thin sections. We showed on tilted images that the OA-B stains DNA throughout the whole thickness of the section. In addition, very low quantities of cytoplasmic DNA were stained by this complex, which is now the best DNA stain used in electron microscopy. Within the nucleoli the DNA was localized in the fibrillar centers, where large clumps of dense chromatin were also visible. In the two plant species intranucleolar chromatin forms a complex network with strands partially linked to chromosomal nucleolar-organizing regions identified by in situ hybridization. This study describes for the first time the spatial arrangement of the intranucleolar chromatin in nucleoli of higher plants using high-resolution techniques.

The spatial organization of epidermal structures: hairy establishes the geometrical pattern of Drosophila leg bristles by delimiting the domains of achaete expression

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 9-20 ◽  
Author(s):  
T.V. Orenic ◽  
L.I. Held ◽  
S.W. Paddock ◽  
S.B. Carroll
Keyword(s):  
Drosophila Melanogaster ◽  
Spatial Organization ◽  
Expression Patterns ◽  
Cell Types ◽  
Precursor Cells ◽  
Geometrical Pattern ◽  
Leg Development ◽  
Puparium Formation ◽  
Late Expression ◽  
Bristle Pattern

The spatial organization of Drosophila melanogaster epidermal structures in embryos and adults constitutes a classic model system for understanding how the two dimensional arrangement of particular cell types is generated. For example, the legs of the Drosophila melanogaster adult are covered with bristles, which in most segments are arranged in longitudinal rows. Here we elucidate the key roles of two regulatory genes, hairy and achaete, in setting up this periodic bristle pattern. We show that achaete is expressed during pupal leg development in a dynamic pattern which changes, by approximately 6 hours after puparium formation, into narrow longitudinal stripes of 3–4 cells in width, each of which represents a field of cells (proneural field) from which bristle precursor cells are selected. This pattern of gene expression foreshadows the adult bristle pattern and is established in part through the function of the hairy gene, which also functions in patterning other adult sense organs. In pupal legs, hairy is expressed in four longitudinal stripes, located between every other pair of achaete stripes. We show that in the absence of hairy function achaete expression expands into the interstripe regions that normally express hairy, fusing the two achaete stripes and resulting in extra-wide stripes of achaete expression. This misexpression of achaete, in turn, alters the fields of potential bristle precursor cells which leads to the misalignment of bristle rows in the adult. This function of hairy in patterning achaete expression is distinct from that in the wing in which hairy suppresses late expression of achaete but has no effect on the initial patterning of achaete expression. Thus, the leg bristle pattern is apparently regulated at two levels: a global regulation of the hairy and achaete expression patterns which partitions the leg epidermis into striped zones (this study) and a local regulation (inferred from other studies on the selection of neural precursor cells) that involves refinement steps which may control the alignment and spacing of bristle cells within these zones.

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