New insights into Plectospira genus (Oomycetes, Straminipila): morphological and molecular analyses

We analyzed the morphology and phylogenetic placement of three isolates of Plectospira belonging to Plectospira gemmifera and P. myriandra that were isolated from soil, water bodies and reservoir sediment. The molecular data are from the partial large subunit and the complete internal transcribed regions of the ribosomal DNA. The genus is characterized by the presence of lobulated zoosporangia and mode of zoospore discharge forming an evanescent cluster in the exit orifice. Our isolates clustered together with the single isolate of the genus available in GenBank, however, our phylogenetic analysis and morphological characterization showed that the isolate identified as Plectospira myriandra CBS 523.87 is possibly another taxon. In addition, P. gemmifera is recorded for the first time in Brazil and its sequences were inserted for the first time in a phylogenetic reconstruction.

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Male identification, generic classification and sexual dimorphism of Micronychus pardus (Kazantsev, 2018) comb. nov. (Coleoptera: Lycidae: Calochrominae)

Zootaxa ◽

10.11646/zootaxa.4657.1.9 ◽

2019 ◽

Vol 4657 (1) ◽

pp. 177-182 ◽

Cited By ~ 2

Author(s):

MICHAL MOTYKA

Keyword(s):

Mitochondrial Dna ◽

Sexual Dimorphism ◽

Species Identification ◽

Dna Sequences ◽

Morphological Characters ◽

Molecular Data ◽

Large Specimen ◽

Molecular Analyses ◽

Almost All ◽

First Time

Almost all net-winged beetles are members of Müllerian complexes and their similarity due to phenotypic coevolution sometimes complicates species identification and generic placement. Therefore, large specimen series, detailed exhaustive examination of morphological characters and molecular data are needed to clarify the taxonomic placement. Using mitochondrial DNA sequences, I investigated the sexual dimorphism and generic placement of the recently described species Calochromus pardus Kazantsev, 2018. I found that the species does not belong in Calochromus Guérin-Méneville, 1833 and all morphological characters and molecular analyses point to its placement in Micronychus Motschulsky, 1861. Therefore, Micronychus pardus (Kazantsev, 2018), comb. nov. is proposed. Additionally, the male is described here for the first time showing the sexual dimorphism in the species. Unlike the females, the males do not superficially resemble members of Xylobanus Waterhouse, 1879 with bright coloured elytral costae and black background, but mimics the sympatrically occurring yellow and black lycids in the genus Cautires Waterhouse, 1879.

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Syntopy of cryptic Phyllomys (Rodentia: Echimyidae) species: description of the karyotype of Phyllomys nigrispinus and an expansion of the geographic distribution of Phyllomys sulinus

Mammalia ◽

10.1515/mammalia-2016-0149 ◽

2018 ◽

Vol 82 (3) ◽

pp. 266-275 ◽

Cited By ~ 1

Author(s):

Ana Cláudia Delciellos ◽

Ana Carolina Loss ◽

Marcia Aguieiras ◽

Lena Geise ◽

Oscar Rocha-Barbosa

Keyword(s):

Atlantic Forest ◽

Molecular Analysis ◽

Dna Sequences ◽

National Park ◽

Phylogenetic Reconstruction ◽

First Record ◽

Molecular Data ◽

Geographic Range ◽

Undescribed Species ◽

First Time

Abstract The echimyid rodents of the genus Phyllomys are medium-sized arboreal spiny rats endemic to the Atlantic Forest of Brazil. The genus is currently composed of 13 species, although there are at least four other undescribed species. The ecology, zoogeography and diversity of the genus are still poorly understood. Here, we provide the first record of Phyllomys sulinus from the Brazilian state of Rio de Janeiro, based on a specimen collected in the Serra da Bocaina National Park, which extends the known geographic range of the species approximately 130 km to the northeast. At this site, P. sulinus occurred in syntopy with the cryptic Phyllomys nigrispinus. We describe the karyotype of P. nigrispinus for the first time and identify two different diploid numbers (2n=84 and 85). We also conducted a molecular analysis of the cytochrome b gene of five specimens, which we identified by phylogenetic reconstruction. Our findings reinforce the importance of molecular data, such as DNA sequences, and karyotypes for the differentiation of cryptic, syntopic species.

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An extraordinary new genus and three new species of Acostemmini (Hemiptera: Cicadellidae: Deltocephalinae) from Madagascar with comments on the morphology and classification of the tribe

Zootaxa ◽

10.11646/zootaxa.3209.1.2 ◽

2012 ◽

Vol 3209 (1) ◽

pp. 28 ◽

Cited By ~ 5

Author(s):

J. N. ZAHNISER ◽

M. W. NIELSON

Keyword(s):

New Species ◽

New Genus ◽

Strong Support ◽

Molecular Data ◽

Morphological Characterization ◽

New Combination ◽

Speci Mens ◽

Three New Species ◽

First Time

The circumscription and morphological characterization of the deltocephaline tribe Acostemmini is revised based on dis-coveries of new and poorly known taxa from recent collections in Madagascar and from examination of the type speci-mens of Alocoelidia fulva Evans, Iturnoria insulana Evans, and Protonesis delegorguei Spinola. Alocoelidia Evans 1954,Caelidioides Signoret 1880, Iturnoria Evans 1954, and Protonesis Spinola 1850 were previously placed in Coelidiinaebut were later removed from the subfamily and until now have been unplaced to subfamily are placed here in Deltocepha-linae: Acostemmini, new placement. A new genus and species, Ikelibeloha cristata gen. n., sp. n., are described that havean unusually modified structure of the head and a large, crested pronotum. Molecular data (28S, Histone H3 genes) wereobtained for Ikelibeloha and Iturnoria, and analyzed with other members of Deltocephalinae. Results of the phylogeneticanalyses show strong support for the monophyletic clade ((Acostemma, Eryapus), (Ikelibeloha, Iturnoria)) and thus a re-lationship between previously described Acostemmini, Ikelibeloha, and Iturnoria has strong statistical support, and aclose relationship is inferred between these and the morphologically similar Alocoelidia, Caelidioides, and Protonesis.Two new species of Alocoelidia, A. maurae sp. n. and A. chasei sp. n. are described, and the genus is redescribed. Themale genitalia of Caelidioides tristis (Signoret), Iturnoria insulana, Alocoelidia fulva, and Acostemella rubra Evans areillustrated and described for the first time. The female genitalia of A. fulva, C. tristis and I. insulana are described and thefirst and second valvulae of C. tristis are illustrated. The habitus and face of Protonesis delegorguei are illustrated and thegenus is redescribed. The characters traditionally used to define Acostemmini are reviewed with notes on their known de-grees of variation, and characters that differentiate Acostemmini from the closely related tribe Stegelytrini are discussed.The type of Malagasiella minima Evans was also examined, and Malagasiella Evans, 1954 is considered a junior syn-onym of Doratulina Melichar, 1903 (Deltocephalinae: Stenometopiini), syn. n. giving the new combination Doratulina minima (Evans) comb. n.

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Phylogenetic position of Coronastrum ellipsoideum and description of Parachlorella hussii sp. nov.

Biologia ◽

10.2478/s11756-011-0056-8 ◽

2011 ◽

Vol 66 (4) ◽

Cited By ~ 14

Author(s):

Christina Bock ◽

Marie Pažoutová ◽

Lothar Krienitz

Keyword(s):

Molecular Data ◽

Phylogenetic Position ◽

Rrna Gene ◽

Combined Approach ◽

Molecular Analyses ◽

Phylogenetic Studies ◽

The Family ◽

Its Rrna Gene ◽

Molecular Phylogenetic ◽

First Time

AbstractFollowing traditional morphological concepts, the genus Coronastrum is considered to be a rare member of the Scenedesmaceae (Chorophyceae). This classification may be called into question when molecular data are taken into account as well. Recent molecular phylogenetic studies revealed the polyphyletic origin of the family Scenedesmaceae within the Chlorophyceae and Trebouxiophyceae. In a combined approach of morphological analyses, SSU/ITS rRNA gene phylogeny and comparison of the ITS secondary structure, we analysed the systematics of Coronastrum strains available in public strain collections. Our molecular analyses revealed a new subclade within the Chlorella clade of the Chlorellaceae consisting of Coronastrum ellipsoideum, two strains with Dictyosphaerium-like morphology and one strain which fits the description of the genus Parachlorella. Four additional strains formed together a new lineage within the genus Parachlorella in the Parachlorella clade of the Chlorellaceae. These strains differ from the already known Parachlorella species in complementary base changes within the ITS2 and are here described for the first time as Parachlorella hussii sp. nov.

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Molecular characterization of three species belongs to the Allocreadioidea, Hemiuroidea and Plagiorchioidea (Platyhelminthes: Trematoda) infecting freshwater fishes in India

Helminthologia ◽

10.1515/helmin-2016-0039 ◽

2016 ◽

Vol 53 (4) ◽

pp. 378-384 ◽

Cited By ~ 2

Author(s):

A. Chaudhary ◽

S. Mukut ◽

H. S. Singh

Keyword(s):

Phylogenetic Reconstruction ◽

Freshwater Fishes ◽

Molecular Data ◽

Trematode Species ◽

Rrna Gene ◽

28S Rrna ◽

Channa Punctata ◽

Digenetic Trematodes ◽

First Time

Summary Three species of digenetic trematodes are redescribed based on specimens collected from the intestine of freshwater fishes of Hastinapur and Meerut (U.P.), India: Allocreadium handiai (Pande, 1937) Madhavi, 1980 (Allocreadioidea: Allocreadiidae) from Mystus tengara (Hamilton, 1822) (Siluri-formes: Bagridae), Genarchopsis goppo Ozaki, 1925 (Hemiuroidea: Derogenidae) and Phyllodistomum chauhani Motwani & Srivastava, 1961 (Plagiorchioidea: Gorgoderidae) from Channa punctata (Bloch, 1793) (Perciformes: Channidae). The three species were subjected to morphological, morphometric and molecular analyses. The morphological study revealed that A. handiai, G. goppo and P. chauhani can be distinguished by their congeners on the basis of their morphology. Partial nucleotide sequences of the 28S ribosomal RNA gene were obtained from the three trematode species and deposited in the GenBank. A phylogenetic reconstruction based on the 28S rRNA gene placed the three studied species within their respective families and their validity is discussed. For the first time molecular data of newly collected material of these species from India were used for confirmation of their validity and to assess their phylogenetic relationships.

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Re-collection of Dermea prunus in China, with a description of D. chinensis sp. nov.

MycoKeys ◽

10.3897/mycokeys.50.32517 ◽

2019 ◽

Vol 50 ◽

pp. 79-91

Author(s):

Ning Jiang ◽

Cheng-Ming Tian

Keyword(s):

Internal Transcribed Spacer ◽

Sequence Data ◽

Large Subunit ◽

Morphological Characteristics ◽

Molecular Data ◽

Generic Concept ◽

Frequent Use ◽

Morphological And Molecular Data ◽

First Time ◽

Asexual Morphs

Dermea was protected against its synonym, Foveostroma, due to its well-circumscribed generic concept and more frequent use. We describe and illustrate Dermeachinensissp. nov. based on its morphological characteristics and a molecular analysis of the internal transcribed spacer (ITS) and large subunit (LSU) sequence data. Dermeachinensis is isolated from Betulaalbosinensis with sexual and asexual morphs and can be distinguished from D.molliuscula on Betula trees by its aseptate and wider ascospores. The connection between the two morphs is proved based on sequence data. Here, we describe the asexual morph of D.pruni for the first time based on morphological and molecular data from the same host and country of origin, and compare it with other species of Prunus.

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Morphological and molecular data confirm the transfer of homostylous species in the typically distylous genus Galianthe (Rubiaceae), and the description of the new species Galianthe vasquezii from Peru and Colombia

PeerJ ◽

10.7717/peerj.4012 ◽

2017 ◽

Vol 5 ◽

pp. e4012

Author(s):

Javier Elias Florentín ◽

Andrea Alejandra Cabaña Fader ◽

Roberto Manuel Salas ◽

Steven Janssens ◽

Steven Dessein ◽

...

Keyword(s):

New Species ◽

Geographical Distribution ◽

Pollen Grains ◽

Morphological Characters ◽

Molecular Data ◽

Diagnostic Features ◽

Molecular Analyses ◽

Generic Concept ◽

Morphological And Molecular Data ◽

First Time

Galianthe (Rubiaceae) is a neotropical genus comprising 50 species divided into two subgenera, Galianthe subgen. Galianthe, with 39 species and Galianthe subgen. Ebelia, with 11 species. The diagnostic features of the genus are: usually erect habit with xylopodium, distylous flowers arranged in lax thyrsoid inflorescences, bifid stigmas, 2-carpellate and longitudinally dehiscent fruits, with dehiscent valves or indehiscent mericarps, plump seeds or complanate with a wing-like strophiole, and pollen with double reticulum, rarely with a simple reticulum. This study focused on two species that were originally described under Diodia due to the occurrence of fruits indehiscent mericarps: Diodia palustris and D. spicata. In the present study, classical taxonomy is combined with molecular analyses. As a result, we propose that both Diodia species belong to Galianthe subgen. Ebelia. The molecular position within Galianthe, based on ITS and ETS sequences, has been supported by the following morphological characters: thyrsoid, spiciform or cymoidal inflorescences, bifid stigmas, pollen grains with a double reticulum, and indehiscent mericarps. However, both species, unlike the remainder of the genus Galianthe, have homostylous flowers, so the presence of this type of flower significantly modifies the generic concept. In this framework, a third homostylous species, Galianthe vasquezii, from the Andean region is also described. Until now, this species remained cryptic under specimens of Galianthe palustris It differs however from the latter by having longer calyx lobes, the presence of dispersed trichomes inside the corolla lobes (vs. glabrous), fruits that are acropetally dehiscent (vs. basipetally dehiscent), and its Andean geographical distribution (vs. Paranaense). Additionally, a lectotype has been chosen for Diodia palustris, Borreria pterophora has been placed under synonymy of Galianthe palustris, and Galianthe boliviana is reported for the first time from Peru. A key of all Galianthe species with indehiscent mericarps is also provided.

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Acanthocotyle gurgesiella n. sp. (Monogenea: Acanthocotylidae) from the deep-sea skate Gurgesiella furvescens (Rajidae) in the south-eastern Pacific

Journal of Helminthology ◽

10.1017/s0022149x17000220 ◽

2017 ◽

Vol 92 (2) ◽

pp. 223-227 ◽

Cited By ~ 5

Author(s):

L.A. Ñacari ◽

F.A. Sepulveda ◽

R. Escribano ◽

M.E. Oliva

Keyword(s):

New Species ◽

Deep Sea ◽

Dna Analysis ◽

Mitochondrial Gene ◽

Large Subunit ◽

Morphological Characteristics ◽

Molecular Data ◽

Full Description ◽

Lsu Rdna ◽

Molecular Analyses

AbstractLittle is known about the diversity of parasites of the deep-sea fish of the world's oceans. Here, a new species of monogenean parasite of the deep-sea skate Gurgesiella furvescens is described. Specimens of parasites were obtained from the skin of two specimens of the dusky finless skate, G. furvescens (Rajidae), in the vicinity of Valparaiso (33°S, 72°W), central Chile, from midwater trawl fishing at depths of 350–450 m. Both morphological and molecular analyses were conducted to provide a full description of the new species, named Acanthocotyle gurgesiella. For the molecular analyses, nuclear large subunit (LSU) rDNA and the mitochondrial gene cytochrome c oxidase 1 (COI) were used. From the morphological analysis and a comparison with the known species of the genus, A. gurgesiella can be identified by a combination of morphological characteristics, including the number of testes, number of radial rows of sclerites in the pseudohaptor, aperture of the genital pore and shape of the vitelline follicles. The results from the DNA analysis indicated that A. gurgesiella has a genetic divergence of 3.2–3.7% (LSU rDNA gene) from A. urolophi, the only congener species for which molecular data are available.

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Astrocoryne cabela, gen. nov. et sp. nov. (Hydrozoa : Sphaerocorynidae), a new sponge-associated hydrozoan

Invertebrate Systematics ◽

10.1071/is16091 ◽

2017 ◽

Vol 31 (6) ◽

pp. 734 ◽

Cited By ~ 5

Author(s):

Davide Maggioni ◽

Paolo Galli ◽

Michael L. Berumen ◽

Roberto Arrigoni ◽

Davide Seveso ◽

...

Keyword(s):

Red Sea ◽

New Genus ◽

Molecular Data ◽

Molecular Evidence ◽

New Genus And Species ◽

Molecular Analyses ◽

The Family ◽

Polyp Stage ◽

Molecular Assessment ◽

First Time

The family Sphaerocorynidae includes two valid genera and five species, most of which have a confusing taxonomic history. Here, a new genus and species, Astrocoryne cabela, gen. et sp. nov., is described from the Maldives and the Red Sea, based on both morphological and molecular evidence. Astrocoryne cabela has an apomorphy represented by the type of tentacles, here named ‘dicapitate’, and consisting of capitate tentacles with a proximal capitulum-like cluster of nematocysts. Molecular analyses confirmed the monophyly of this species, as well as its belonging to the Sphaerocorynidae, together with Sphaerocoryne spp. and Heterocoryne caribbensis Wedler & Larson, 1986, for which we present molecular data for the first time. Moreover, the high divergence of A. cabela from other species of the family justifies the establishment of a new genus. Interestingly, specimens from the Maldives and the Red Sea showed marked morphological variation in the polyp stage, although only a slight genetic divergence was detected. This study highlights that a comprehensive morpho-molecular assessment of Sphaerocorynidae is strongly needed in order to clarify the taxonomic issues and the diversity of this taxon.

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Molecular Data Reveal Unrecognized Diversity in the European Ganoderma resinaceum

Forests ◽

10.3390/f11080850 ◽

2020 ◽

Vol 11 (8) ◽

pp. 850

Author(s):

Kateřina Náplavová ◽

Terézia Beck ◽

Peter Pristaš ◽

Svetlana Gáperová ◽

Martin Šebesta ◽

...

Keyword(s):

Sequence Data ◽

Large Subunit ◽

Elongation Factor ◽

Molecular Data ◽

Urban Trees ◽

Translation Elongation ◽

Ionization Time ◽

Basidiomycetous Fungus ◽

Flight Mass Spectrometry ◽

First Time

Ganoderma resinaceum Boud. is commonly found in Mediterranean region, but rarely in Western, Central or Eastern Europe. It is a parasitic basidiomycetous fungus causing stem decay—especially in urban trees. A collection of nine fungal specimens from Slovakia (Central Europe), morphologically identified as G. resinaceum, was recently studied on the basis of sequence data from the internal transcribed spacer (ITS) regions. Analyses showed that the collections clustered into two separate groups. In this study—for the first time—the sequences of other molecular markers, namely partial translation elongation factor (tef1-α) region and partial 25S large subunit ribosomal RNA gene (25S LSU rRNA), as well as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI–TOF MS) were obtained and used to evaluate the genetic variability of G. resinaceum. All these analyses confirm the existence of two previously unrecognized genotypes within the morphospecies.

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