Phylogenetic, cytological and morphological comparisons of Oxalis subsect. Oxalis (Oxalidaceae) in East Asia

Phytotaxa ◽  
2017 ◽  
Vol 324 (3) ◽  
pp. 266
Author(s):  
SATOSHI AOKI ◽  
TETSUO OHI-TOMA ◽  
PAN LI ◽  
CHENGXIN FU ◽  
JIN MURATA
Keyword(s):  
East Asia ◽  
Geographical Area ◽  
Flow Cytometric Analysis ◽  
Mainland China ◽  
Taxonomic Revision ◽  
Flow Cytometric ◽  
Clade B ◽  
Clade C ◽  
Molecular Phylogenetic

To assess the classification of East Asian taxa in Oxalis subsect. Oxalis, samples from a wide geographical area were examined using molecular phylogenetic, cytological and morphological analyses. The phylogenetic analysis showed the early branching of O. leucolepis and O. obtriangulata in East Asia, followed by that of O. acetosella and O. oregana in North America. The remaining Eurasian samples were divided into four clades: taxa from Mainland China and Taiwan (Clade A); O. acetosella from Eurasia including Japan (Clade B); O. griffithii from Japan (Clade C); and O. griffithii plus some O. acetosella from Japan (Clade D). Therefore, O. acetosella and O. griffithii are not monophyletic species. In the flow cytometric analysis, three diploid groups with different relative genome sizes were distinguished, and were placed in each of Clades A, B, and C plus D; tetraploids were found in Clades C and D. Morphologically, most pairs of samples had distinguishing characters that were informative for delimitation. However, the characters of O. griffithii in Clades A and C (including tetraploids) were not informative, and diploids and tetraploids in Clade C did not morphologically differ. These results demonstrate the necessity for a taxonomic revision of this subsection.

Utility of flow cytometric analysis of mast cells in the diagnosis and classification of adult mastocytosis

2001 ◽  
Vol 25 (7) ◽  
pp. 563-570 ◽  
Author(s):  
Luis Escribano ◽  
Beatriz Dı́az-Agustı́n ◽  
Carmen Bellas ◽  
Raquel Navalón ◽  
Rosa Nuñez ◽  
...  
Keyword(s):  
Mast Cells ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Diagnosis And Classification

scholarly journals Multigene phylogeny and taxonomic revision of American shrimps of the genus Cryphiops Dana, 1852 (Decapoda, Palaemonidae) implies a proposal for reversal of precedence with Macrobrachium Spence Bate, 1868

ZooKeys ◽  
2021 ◽  
Vol 1047 ◽  
pp. 155-198
Author(s):  
Fernando L. Mantelatto ◽  
Leonardo G. Pileggi ◽  
João A. F. Pantaleão ◽  
Célio Magalhães ◽  
José Luis Villalobos ◽  
...  
Keyword(s):  
Taxonomic Revision ◽  
Morphological Characters ◽  
Disjunct Distribution ◽  
Evolutionary Relationships ◽  
Multigene Phylogeny ◽  
Important Species ◽  
Current Classification ◽  
Molecular Phylogenetic ◽  
South Brazil

The freshwater shrimp genus Cryphiops Dana, 1852 has a disjunct distribution in North (Mexico) and South (Brazil, Chile) America, and is composed of only six species. The current classification of genera in the Palaemonidae is controversial, based on variable morphological characters, and still far from a clear definition. Cryphiops differs from the speciose genus Macrobrachium Spence Bate, 1868 only by the absence of the hepatic spines on the carapace. Previous studies with a limited dataset suggested the necessity to link morphology and phylogeny to create an internal rearrangement in the genus to resolve the paraphyletic status. Through a molecular phylogenetic approach, the evolutionary relationships are inferred based on four (mitochondrial and nuclear) genes, among all recognized species of Cryphiops and, in combination with a taxonomic revision, a rearrangement in the systematics of the genus is suggested. The absence of hepatic spines on the carapace, the only character used to separate the genus Cryphiops, is subjective and should be considered as a homoplasy. This implies that Cryphiops and Macrobrachium are subjective synonyms and, because the latter genus is much more diverse and widely known, with several economically important species, to avoid confusion and disturbance in nomenclatural stability and keep universality, a proposal for the priority of the older synonym (Cryphiops) to be partially suppressed in favor of maintaining the prevailing use of the younger synonym (Macrobrachium) is presented. As the species of Cryphiops should be accommodated in the genus Macrobrachium, new names to replace three preoccupied specific names that, by this action, resulted to be secondary homonyms are offered.

Lymphoproliferative Neoplasms of the Lung: A Review

2013 ◽  
Vol 137 (3) ◽  
pp. 382-391 ◽  
Author(s):  
Josette William ◽  
Daina Variakojis ◽  
Anjana Yeldandi ◽  
Kirtee Raparia
Keyword(s):  
T Lymphocyte ◽  
Personal Experience ◽  
Flow Cytometric Analysis ◽  
Prognostic Features ◽  
Flow Cytometric ◽  
Primary Pulmonary Lymphoma ◽  
Pulmonary Lymphoma ◽  
Genetic Features ◽  
Diagnosis And Classification

Context.—Diagnosis and classification of lymphomas are based on the morphologic, immunologic, and genetic features that the lesional cells share with their normal B and T lymphocyte counterparts. Primary pulmonary lymphomas account for 0.3% of primary lung neoplasms and less than 0.5% of all lymphomas. Objective.—To describe and summarize the clinical and histopathologic features of the primary pulmonary lymphoma and secondary involvement of the lung by lymphoma. Data Sources.—Peer-reviewed published literature and personal experience. Conclusions.—Diagnosis of clonal lymphoid proliferations in the lung has evolved owing to the greater utility of molecular and flow cytometric analysis of tissue. Further studies are needed to best define the clinical and prognostic features, as well as search for targeted therapy for these patients with rare neoplasms.

Molecular phylogeny and classification of Chelidurella Verhoeff, stat. restit. (Dermaptera: Forficulidae)

2020 ◽  
pp. 1-37
Author(s):  
Markéta Kirstová ◽  
Robin Kundrata ◽  
Petr Kočárek
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogeny ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Two New Species ◽  
Molecular Phylogenetic

Abstract We present herein the first phylogenetic analysis of the genus Chelidura and the taxonomic revision of the genus Chelidurella, stat. restit., based on DNA sequences. The results confirm the generic status of Chelidurella Verhoeff, 1902 and Mesochelidura Verhoeff, 1902, and they are removed from the synonymy with Chelidura and reinstated as valid genera. Many individual Chelidurella species are defined based on the combination of a few variable characters on the pygidium and forceps, and the systematics and phylogeny of this genus are unclear. The validity of most of the species is revisited here by molecular phylogenetic analyses, and individual morphological characters are evaluated for their relevance in the identification of all described species. We describe two new species to science, Chelidurella galvagnii Kirstová & Kočárek, sp. nov. from Austria, and C. pseudovignai Kočárek & Kirstová, sp. nov. from Italy and Austria; two species, C. guentheri Galvagni, 1994 and C. tatrica Chládek, 2017 are newly synonymized. Critical diagnostic characters are illustrated, and an identification key for males of Chelidurella is provided.

scholarly journals FLOW CYTOMETRY IN MYELODYSPLASTIC SYNDROME. STEP FORWARD.

2019 ◽  
Vol 141 (7-8) ◽  
pp. 214-219
Keyword(s):  
Bone Marrow ◽  
Myelodysplastic Syndrome ◽  
Flow Cytometric Analysis ◽  
Lymphoid Tissues ◽  
Hematopoietic Stem ◽  
Diagnosis And Prognosis ◽  
Flow Cytometric ◽  
Increased Risk ◽  
Made In

Myelodysplastic syndrome (MDS) represents a heterogeneous group of clonal diseases originating from altered (malignant) hematopoietic stem cells. Clinically, it manifests as cytopenia in peripheral blood, dysplastic changes in one or more cell lines in bone marrow and increased risk of evolution to acute myeloid leukemia (AML). Acquired chromosomal aberrations are detected in 40-50% of MDS patients. According to WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (Lion 2008), MDS diagnosis is based on the morphological interpretation of found dysplastic changes, the number of blasts, the presence of ring sideroblasts in bone marrow and the established cytogenetic abnormalities. The progress made in recent years in immunophenotyping of hematopoietic progenitor cells and mature cells of dysplastic bone marrow gives to multiparameter flow cytometric analysis (MFC), although initially included as an optional technique, an opportunity to become a standard part of the integrated MDS diagnosis and prognosis.

Apoptotic Effects of Melittin on 4T1 Breast Cancer Cell Line is associated with Up Regulation of Mfn1 and Drp1 mRNA Expression

2020 ◽  
Vol 20 (7) ◽  
pp. 790-799 ◽  
Author(s):  
Farnaz D. Moghaddam ◽  
Pejman Mortazavi ◽  
Somayeh Hamedi ◽  
Mohammad Nabiuni ◽  
Nasim H. Roodbari
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Hemolytic Activity ◽  
Breast Cancer Cells ◽  
Flow Cytometric Analysis ◽  
Control Group ◽  
Flow Cytometric ◽  
4T1 Cells ◽  
4T1 Breast Cancer ◽  
Apoptotic Effects

Background and Purpose: Melittin, as the main ingredient of honeybee venom, that has shown anticancer properties. The present study aimed at investigating the cytotoxic impacts of melittin on 4T1 breast cancer cells. Methods: Hemolytic activity of different concentrations (0.125, 0.25, 0.5, 1, 2, 4, 8μg/ml) of melittin was assayed and then cytotoxicity of selected concentrations of melittin (2, 4, 8, 16, 32, and 64μg/ml), 2 and 4μg/ml of cisplatin and 0.513, 0.295 and 0.123μg/ml of doxorubicin was evaluated on 4T1 cells using MTT assay. We used Morphological evaluation and flow cytometric analysis was used. Real time PCR was also used to determine mRNA expression of Mfn1 and Drp1 genes. Results: All compounds showed anti-proliferative effects on the tumor cell line with different potencies. Melittin had higher cytotoxicity against 4T1 breast cancer cells (IC50= 32μg/ml-72h) and higher hemolytic activity (HD50= 1μg/ml), as compared to cisplatin and doxorubicin. Mellitin at 16 and 32μg/ml showed apoptotic effects on 4T1 cells according to the flow cytometric analysis. The Real time PCR analysis of Drp1 and Mfn1 expression in cells treated with 16μg/ml of melittin revealed an up-regulation in Drp1 and Mfn1 genes mRNA expression in comparison with control group. Treatment with 32μg/ml of melittin was also associated with a rise in mRNA expression of Drp1 and Mfn1 as compared to the control group. Conclusion: The results of this study showed that melittin has anticancer effects on 4T1 cell lines in a dose and time dependent manner and can be a good candidate for further research on breast cancer treatment.

Comparing Post-communist Authoritarianism in Russia and China

2018 ◽  
Author(s):  
Cheng Chen
Keyword(s):  
Comparative Study ◽  
East Asia ◽  
Eastern Europe ◽  
Case Studies ◽  
Single Case ◽  
Full Range ◽  
Geographical Area ◽  
Context Sensitive ◽  
Elite Politics ◽  
Significant Divergence

The studies of post-communist Russia and China have traditionally been dominated by single-case studies and within-region comparisons. This chapter explores why the CAS of post-communist Russia and China is difficult, why it is rare, and how it could yield significant and unique intellectual payoffs. The cross-regional comparative study of anti-corruption campaigns in contemporary Russia and China is used as an example in this chapter to argue that a well-matched and context-sensitive comparison could reveal significant divergence in the elite politics and institutional capacities of these regimes that would otherwise likely be obscured by single-case studies or studies restricted to one single geographical area such as “Eastern Europe” or “East Asia.” By breaking Russia and China out of their respective “regions,” the CAS perspective thus enables us to better capture the full range of existing diversity of post-communist authoritarianism.

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