Species delimitation and taxonomic revision of Oxyopes (Araneae: Oxyopidae) of Taiwan, with description of two new species

Zootaxa ◽  
2021 ◽  
Vol 4927 (1) ◽  
pp. 58-86
Author(s):  
YING-YUAN LO ◽  
REN-CHUNG CHENG ◽  
CHUNG-PING LIN
Keyword(s):  
Genetic Divergence ◽  
Species Delimitation ◽  
Gap Analysis ◽  
Taxonomic Revision ◽  
Ecological Data ◽  
Taichung City ◽  
Taipei City ◽  
Molecular Phylogenetic ◽  
Coi Sequences ◽  
First Time

This study revised the spider genus Oxyopes Latreille, 1804 in Taiwan and delineated the species boundaries based on morphological and molecular characters. A total of seven Oxyopes spiders were recognized, including two newly described species, O. hasta sp. nov. and O. taiwanensis sp. nov. Oxyopes fujianicus Song & Zhu 1993 from Yilan County, Nantou County, and Kaohsuing City, and O. striagatus Song 1999 from New Taipei City, Taichung City, Nantou County, and Kaohsiung City were recorded for the first time in Taiwan. An identification key and a distributional map of Taiwanese Oxyopes species were provided. Partial COI sequences were obtained for molecular phylogenetic and species delimitation analyses. Maximum likelihood and Bayesian phylogenies, and DNA barcoding gap analysis supported morphologically defined species. However, molecular species delimitation based on Automatic Barcode Gap Discovery (ABGD), PID (Liberal), and generalized mixed Yule coalescent (GMYC) were incongruent in species assignment. The results showed that the interspecific genetic divergence between O. sertatus and O. taiwanensis was relatively low (1.28 ± 0.43%), and the intraspecific genetic divergence of O. striagatus was relatively high (1.69 ± 0.35%). Ecological data, additional samples and genetic loci are required to further examine the level of reproductive isolation and patterns of population genetic structure in Taiwanese Oxyopes. 

Molecular insights into the phylogenetic placement of the poorly known genus Niceforonia Goin & Cochran, 1963 (Anura: Brachycephaloidea)

Zootaxa ◽  
2018 ◽  
Vol 4514 (4) ◽  
pp. 487
Author(s):  
ANDRÉS R. ACOSTA-GALVIS ◽  
JEFFREY W. STREICHER ◽  
LUIGI MANUELLI ◽  
TRAVIS CUDDY ◽  
RAFAEL O. DE SÁ
Keyword(s):  
Phylogenetic Analysis ◽  
New World ◽  
Taxonomic Revision ◽  
Phylogenetic Inference ◽  
Genetic Distances ◽  
Molecular Phylogenetic Analysis ◽  
Phylogenetic Placement ◽  
Molecular Phylogenetic ◽  
First Time

Among New World direct-developing frogs belonging to the clade Brachycephaloidea (= Terraranae), there are several genera with uncertain phylogenetic placements. One notable example is the genus Niceforonia Goin & Cochran 1963, which includes three species that are endemic to Colombia. Three specimens of the species Niceforonia nana were collected and for the first time the genus is included in a molecular phylogenetic analysis of mitochondrial (mtDNA; 12S and 16S) and nuclear (nucDNA; TYR and RAG1) markers. Molecular phylogenetic inference based on concatenated and separate mtDNA and nucDNA analyses recovered Niceforonia nana nested within Hypodactylus Hedges et al. 2008, rendering the latter genus paraphyletic. Consequently, herein we place the genus Hypodactylus in the synonymy of Niceforonia to resolve the paraphyly and place Niceforonia in the subfamily Hypodactylinae. Based on our revised concept of the genus Niceforonia we conducted preliminary morphological comparisons using specimens and literature descriptions. Finally, Nicefornia nana is quite divergent from other species of Niceforonia (uncorrected genetic distances of ca. 10% 16S and 7% TYR) suggesting that further taxonomic revision may be warranted. 

scholarly journals Establishment of six new Rhabdoblatta species (Blattodea, Blaberidae, Epilamprinae) from China

ZooKeys ◽  
2019 ◽  
Vol 851 ◽  
pp. 27-69
Author(s):  
Rong Yang ◽  
Zhenzhen Wang ◽  
Yanshuang Zhou ◽  
Zongqing Wang ◽  
Yanli Che
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Species ◽  
Species Delimitation ◽  
Female Genitalia ◽  
Morphological Data ◽  
Coi Sequences ◽  
First Time

This study examined 504 Rhabdoblatta specimens sampled from China, of which, 86 Rhabdoblatta specimens were used for COI sequencing. A phylogenetic analysis using the ML method and MOTUs estimations by ABGD and GMYC based on COI sequences was performed. Eighteen Rhabdoblatta species were identified when these data were combined with morphological data. Six new species were established among these samples, i.e., Rh.similsinuatasp. n., Rh.densimaculatasp. n., Rh.gyroflexasp. n., Rh.chaulformissp. n., Rh.maculatasp. n., and Rh.ecarinatasp. n. For the first time, females including female genitalia of 14 known Rhabdoblatta species are described worldwide. Our study shows that combining molecular species delimitation methods with morphological data helps to delimit species and understand cockroach biodiversity.

Can morphology and chromosome number contribute to species delimitation? A case in the Hibiscus trionum complex (Tribe Hibisceae, Malvaceae)

Phytotaxa ◽  
2019 ◽  
Vol 416 (4) ◽  
pp. 278-286
Author(s):  
MOHAMED OWIS BADRY ◽  
JENNIFER A. TATE ◽  
PRASHANT JOSHI ◽  
AHMED MAHMOUD ABBAS ◽  
SOHAIR THABET HAMED ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Species Delimitation ◽  
Taxonomic Revision ◽  
Herbarium Specimens ◽  
Chromosome Counts ◽  
Comprehensive Description ◽  
Hibiscus Trionum ◽  
The World ◽  
First Time

A taxonomic revision of Hibiscus trionum from Egypt was undertaken using morphological and cytological studies of field collections and herbarium specimens. The data indicate that all specimens so far collected in Egypt belong to H. tridactylites, rather than H. trionum. This paper includes a comprehensive description of H. tridactylites, its occurrence, a comparison with other species in the H. trionum complex, and comments on habitat, along with images for easy identification. In addition, chromosome counts for this species from Egypt have been determined for the first time, which add to existing information on chromosome counts for the species from different regions of the world.

An enigma no more: an integrated taxonomic revision of Aenigmetopia Malloch reveals novel phylogenetic placement and four new species (Diptera : Sarcophagidae : Miltogramminae)

2020 ◽  
Author(s):  
Nikolas P. Johnston ◽  
James F. Wallman ◽  
Krzysztof Szpila ◽  
Thomas Pape
Keyword(s):  
New Species ◽  
Taxonomic Revision ◽  
Single Species ◽  
Species Level ◽  
Morphological Data ◽  
Morphological Evidence ◽  
Phylogenetic Placement ◽  
Molecular Phylogenetic ◽  
First Time ◽  
Flesh Flies

Aenigmetopia Malloch is the only endemic genus of miltogrammine flesh flies (Diptera:Sarcophagidae) in Australia and, until now it has been known from a single species, A. fergusoni Malloch. This study constitutes the first comprehensive taxonomic revision of Aenigmetopia. Four new species, Aenigmetopia amissa, sp. nov., A. corona, sp. nov., A. kryptos, sp. nov. and A. pagoni, sp. nov., are described through the integration of molecular and morphological data and characters for genus- and species-level diagnoses are given. Aenigmetopia is included in a molecular phylogenetic analysis for the first time and the genus emerges as the sister taxon to Metopia Meigen, in agreement with morphological evidence.

scholarly journals Fine-scale species delimitation: speciation in process and periodic patterns in nudibranch diversity

ZooKeys ◽  
2020 ◽  
Vol 917 ◽  
pp. 15-50 ◽  
Author(s):  
Tatiana Korshunova ◽  
Klas Malmberg ◽  
Jakov Prkić ◽  
Alen Petani ◽  
Karin Fletcher ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Geographic Range ◽  
Northern Europe ◽  
Fine Scale ◽  
Ecological Data ◽  
Periodic Patterns ◽  
Separate Species ◽  
Integrated Method ◽  
Ecological Information ◽  
First Time

Using the nudibranch genus Amphorina as a model, ongoing speciation is demonstrated, as well as how periodic-like patterns in colouration can be included in an integrated method of fine-scale species delimitation. By combining several methods, including BPP analysis and the study of molecular, morphological, and ecological data from a large number of specimens within a broad geographic range from northern Europe to the Mediterranean, five species are recognised within the genus Amphorina, reviewed here for the first time. Two new species from the southwestern coast of Sweden are described, A. viriolasp. nov. and A. andrasp. nov. Evidence is provided of a recent speciation process between the two closely related, yet separate, species which inhabit the same geographic localities, but demonstrate strict water depth differentiation, with one species inhabiting the shallow brackish top layer above the halocline and the other species inhabiting the underlying saltier water. The results presented here are of relevance for currently debated issues such as conservation in relation to speciation, fine species delimitation, and integration of molecular, morphological and ecological information in biodiversity studies. The periodic approach to taxonomy has considerable practical potential for various organismal groups.

scholarly journals Evidence of cryptic diversity in freshwater Macrobrachium prawns from Indochinese riverine systems revealed by DNA barcode, species delimitation and phylogenetic approaches

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252546
Author(s):  
Warut Siriwut ◽  
Ekgachai Jeratthitikul ◽  
Somsak Panha ◽  
Ratmanee Chanabun ◽  
Peng Bun Ngor ◽  
...  
Keyword(s):  
Genetic Divergence ◽  
Species Delimitation ◽  
Gap Analysis ◽  
Molecular Taxonomy ◽  
Dna Barcode ◽  
Freshwater Ecosystems ◽  
Nominal Species ◽  
Morphological Examination ◽  
High Genetic Diversity ◽  
Riverine Systems

The diversity of Indochinese prawns in genus Macrobrachium is enormous due to the habitat diversification and broad tributary networks of two river basins: the Chao Phraya and the Mekong. Despite long-standing interest in SE-Asian decapod diversity, the subregional Macrobrachium fauna is still not yet comprehensively clarified in terms of taxonomic identification or genetic diversification. In this study, integrative taxonomic approaches including morphological examination, DNA barcoding, and molecular species delimitation were used to emphasize the broad scale systematics of Macrobrachium prawns in Indochina. Twenty-seven nominal species were successfully re-verified by traditional and molecular taxonomy. Barcode gap analysis supported broad overlapping of species boundaries. Taxonomic ambiguity of several deposited samples in the public database is related to inter- and intraspecific genetic divergence as indicated by BOLD discordance. Diagnostic nucleotide positions were found in six Macrobrachium species. Eighteen additional putative lineages are herein assigned using the consensus of species delimitation methods. Genetic divergence indicates the possible existence of cryptic species in four morphologically complex and wide-ranging species: M. lanchesteri, M. niphanae, M. sintangense, and some members of the M. pilimanus group. The geographical distribution of some species supports the connections and barriers attributed to paleo-historical events of SE-Asian rivers and land masses. Results of this study show explicitly the importance of freshwater ecosystems in Indochinese subregions, especially for the Mekong River Basin due to its high genetic diversity and species composition found throughout its tributaries.

Taxonomic revision of the genus Pseudocodon (Campanulaceae) based on character analysis and molecular phylogeny

Phytotaxa ◽  
2015 ◽  
Vol 204 (1) ◽  
pp. 49 ◽  
Author(s):  
QIANG WANG ◽  
DE-YUAN HONG
Keyword(s):  
Phylogenetic Analysis ◽  
Species Delimitation ◽  
Nuclear Dna ◽  
Taxonomic Revision ◽  
Field Trips ◽  
New Combination ◽  
Molecular Phylogenetic ◽  
Established Genus ◽  
One New Species ◽  
Made In

All taxa of the newly established genus Pseudocodon (= Codonopsis subg. Pseudocodonopsis), except for Pseudocodon rosulatus, form a complicated group extremely controversial on species delimitation. In the present study, numerous field trips were made, 29 populations were observed, and over 800 specimens of the genus preserved in 18 herbaria were critically examined. Statistical analysis of the characters was carried out based on 148 samples from specimens and 16 field populations. Four fragments of chloroplast DNA (atpB, matK, rbcL, petD with petB-petD spacer) and ITS of nuclear DNA were used in the molecular phylogenetic analysis of 40 samples from specimens and 22 field populations. A taxonomic revision of the genus Pseudocodon is presented on the basis of these studies. We recognize eight species: Pseudocodon convolvulaceus, Pse. graminifolius, Pse. grey-wilsonii, Pse. hirsutus, Pse. petiolatus, Pse. retroserratus, Pse. rosulatus, and Pse. vinciflorus. In the present study, one new species and one new subspecies are described, and one new combination is made. In addition, nine taxa are treated as synonyms, and six lectotypes are designated.

First Records of Bufo luchunnicus (Yang et Rao, 2008) and Amolops wenshanensis Yuan, Jin, Li, Stuart et Wu, 2018 (Anura: Bufonidae, Ranidae) from Vietnam

2020 ◽  
Vol 27 (2) ◽  
pp. 81-86
Author(s):  
Cuong The Pham ◽  
Minh Duc Le ◽  
Chung Van Hoang ◽  
Anh Van Pham ◽  
Thomas Ziegler ◽  
...  
Keyword(s):  
Natural History ◽  
Genetic Divergence ◽  
Morphological Data ◽  
Type Series ◽  
Lao Cai ◽  
First Time ◽  
Nd2 Gene ◽  
Quang Ninh

We record two species of amphibians for the first time from Vietnam: Bufo luchunnicus from Lao Cai and Son La provinces and Amolops wenshanensis from Quang Ninh Province. Morphologically, the Vietnamese representatives of B. luchunnicus resemble the type series from China. The specimen of A. wenshanensis from Vietnam slightly differs from the type series from China by having a smaller size (SVL 33.2 mm vs. 35.7 – 39.9 mm in males) and the presence of distinct transverse bands on the dorsal surfaces of limbs. Genetic divergence between the sequence of the Vietnamese specimen and those of A. wenshanensis from China available from GenBank is 1.2 – 1.6% (ND2 gene). In addition, morphological data and natural history notes of aforementioned species are provided from Vietnam.

scholarly journals Disentangling relationships among eastern Mediterranean Cymbalaria including description of a novel species from the southern Peloponnese (Greece)

2021 ◽  
Vol 307 (2) ◽  
Author(s):  
Pau Carnicero ◽  
Núria Garcia-Jacas ◽  
Llorenç Sáez ◽  
Theophanis Constantinidis ◽  
Mercè Galbany-Casals
Keyword(s):  
Eastern Mediterranean ◽  
Phylogenetic Analyses ◽  
Taxonomic Revision ◽  
Distribution Patterns ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Long Distance ◽  
Aegean Islands ◽  
Molecular Phylogenetic

AbstractThe eastern Mediterranean basin hosts a remarkably high plant diversity. Historical connections between currently isolated areas across the Aegean region and long-distance dispersal events have been invoked to explain current distribution patterns of species. According to most recent treatments, at least two Cymbalaria species occur in this area, Cymbalaria microcalyx and C. longipes. The former comprises several intraspecific taxa, treated at different ranks by different authors based on morphological data, evidencing the need of a taxonomic revision. Additionally, some populations of C. microcalyx show exclusive morphological characters that do not match any described taxon. Here, we aim to shed light on the systematics of eastern Mediterranean Cymbalaria and to propose a classification informed by various sources of evidence. We performed molecular phylogenetic analyses using ITS, 3’ETS, ndhF and rpl32-trnL sequences and estimated the ploidy level of some taxa performing relative genome size measures. Molecular data combined with morphology support the division of traditionally delimited C. microcalyx into C. acutiloba, C. microcalyx and C. minor, corresponding to well-delimited nrDNA lineages. Furthermore, we propose to combine C. microcalyx subsp. paradoxa at the species level. A group of specimens previously thought to belong to Cymbalaria microcalyx constitute a well-defined phylogenetic and morphological entity and are described here as a new species, Cymbalaria spetae. Cymbalaria longipes is non-monophyletic, but characterized by being glabrous and diploid, unlike other eastern species. The nrDNA data suggest at least two dispersals from the mainland to the Aegean Islands, potentially facilitated by marine regressions.

Taxonomic revision of Cylindera Westwood, 1831 subgenus Parmecus Motschulsky, 1864 stat. rest., stat. nov. (Coleoptera: Carabidae: Cicindelinae) with the description of one new species from Yunnan Province, China

Zootaxa ◽  
2019 ◽  
Vol 4706 (1) ◽  
pp. 48-70
Author(s):  
ANDREY V. MATALIN
Keyword(s):  
New Species ◽  
Type Species ◽  
Yunnan Province ◽  
Taxonomic Revision ◽  
Jammu And Kashmir ◽  
Tiger Beetle ◽  
Indian State ◽  
Chinese Province ◽  
First Time ◽  
One New Species

Within the Asiatic tiger beetle fauna, Parmecus Motschulsky, 1864 stat. rest., stat. nov., is reestablished as a subgenus of Cylindera Westwood, 1831 with Cylindera (Parmecus) dromicoides (Chaudoir, 1852), as its type species, and the lectotype and paralectotypes of Cicindela dromicoides Chaudoir, 1852 are designated as well. Two other species are included, Cylindera (Parmecus) armandi (Fairmaire, 1886), from the Himalayan Region, and Cylindera (Parmecus) mosuoa, sp. nov., from Yunnan, China. Cylindera (Parmecus) as a subgenus is characterized, a key to identify its species is provided, and its species composition is discussed. Cylindera (Parmecus) dromicoides (Chaudoir, 1852) is newly recorded from Pakistan and the Indian state of Jammu and Kashmir, while Cylindera (Parmecus) armandi (Fairmaire, 1886) is recorded for the first time from the Chinese province of Sichuan. The records of C. armandi from Bhutan, as well as C. dromicoides from Yunnan Province (China) are rejected due to erroneous identifications. 

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