scholarly journals Pyrin Gene Polymorphisms and <i>H. pylori</i>-associated Dyspepsia: A Sri Lankan Study

2021 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Yashodha Weerasinghe ◽  
Chinthika Gunasekara ◽  
Manjula Weerasekera ◽  
Surangi Jayakody ◽  
Bimalka Seneviratne ◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Sri Lankan ◽  
H Pylori

scholarly journals Genetic Polymorphisms of CXCL8 (−251) Are Associated with the Susceptibility of Helicobacter pylori Infection Increased the Risk of Inflammation and Gastric Cancer in Thai Gastroduodenal Patients

2019 ◽  
Author(s):  
Wongwarut Boonyanugomol ◽  
Kamolchanok Rukseree ◽  
Worrarat Kongkasame ◽  
Prasit Palittapongarnpim ◽  
Seung-Chul Baik ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Cancer Progression ◽  
Chemokine Receptor ◽  
Gene Polymorphisms ◽  
Inflammatory Responses ◽  
Increased Risk ◽  
Cxc Chemokine ◽  
H Pylori

CXC Chemokine Ligand 8 (CXCL8) plays an important role in gastric inflammation and in the progression of gastric cancer induced by Helicobacter pylori (H. pylori) infection. The association of CXCL8, CXC Chemokine Receptor 1 (CXCR1), and CXC Chemokine Receptor 2 (CXCR2) polymorphisms with H. pylori infection and gastric cancer progression needs to be investigated in a population within an enigma area consisting of multiple ethnicities, such as Thailand. To analyze the relative risk of H. pylori infection and gastric cancer among Thai gastroduodenal patients, gene polymorphisms in CXCL8 (promoter region -251) and in CXCR1 and CXCR2 (receptors for CXCL8) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific-PCR (AS-PCR). We also determined the presence of cytotoxin-associated gene A (cagA) in Thai patients with H. pylori infection. Correlation between the CXCL8 (-251) polymorphism and CXCL8 gene expression was evaluated by quantitative reverse transcriptase-PCR (qRT-PCR). We found a significant association between the T/A and A/A genotypes of CXCL8 (-251) with H. pylori infection. However, no significant correlation was found between the CXCR1 (+2607) and CXCR2 (+1208) gene polymorphisms with H. pylori infection among Thai gastroduodenal subjects. Within the H. pylori-infected group of Thai gastroduodenal patients, no significant differences in cagA were observed. In addition, the A/A genotype of CXCL8 (-251) significantly correlated with the risk of gastric cancer and correlated with higher CXCL8 gene expression levels in Thai gastroduodenal patients. These results suggest that CXCL8 (-251) polymorphisms are associated with H. pylori infection, an increased risk of stronger inflammatory responses, and gastric cancer in Thai gastroduodenal patients.  

scholarly journals SY13-2-2 * IMPULSITIVY PERSONALITY TRAITS AND GENE POLYMORPHISMS ASSOCIATED WITH HEROIN DEPENDENCE IN A SRI LANKAN POPULATION

2014 ◽  
Vol 49 (suppl 1) ◽  
pp. i13-i13
Author(s):  
S. Alfreda ◽  
L. O. Dissabandara ◽  
N. J. Loxton ◽  
A. M. Ho ◽  
P. R. Dodd ◽  
...  
Keyword(s):  
Personality Traits ◽  
Gene Polymorphisms ◽  
Heroin Dependence ◽  
Sri Lankan

scholarly journals The Influence of IL-1B Gene Polymorphisms on H. pylori Infection and Triple Treatment Response Among Jordanian Population

2020 ◽  
Vol Volume 13 ◽  
pp. 139-145
Author(s):  
Muhamad A. K. Shakhatreh ◽  
Omar F. Khabour ◽  
Karem H. Alzoubi ◽  
Mohammed N. BaniHani ◽  
Ahmed Abu-Siniyeh ◽  
...  
Keyword(s):  
Treatment Response ◽  
Gene Polymorphisms ◽  
Jordanian Population ◽  
H Pylori ◽  
Triple Treatment ◽  
Em Gene

scholarly journals Association Between Vitamin D Receptor Gene Polymorphisms and Helicobacter Pylori Infection

2020 ◽  
Vol 10 (1) ◽  
pp. 8-14
Author(s):  
Amal A. Mohamed ◽  
Shorouk Moussa ◽  
Mahmoud M. Shaheen ◽  
Sherief Abd-Elsalam ◽  
Rehab Ahmed ◽  
...  
Keyword(s):  
Vitamin D ◽  
Helicobacter Pylori ◽  
Vitamin D Receptor ◽  
Gene Polymorphisms ◽  
Gastrointestinal Symptoms ◽  
Upper Gastrointestinal Endoscopy ◽  
Positive Group ◽  
Biopsy Specimens ◽  
H Pylori ◽  
Upper Gastrointestinal

Background & Aims: Human genetic polymorphism has been reported in the susceptibility and clinical development of infection. In this regard, this study aimed to investigate the link between Vitamin D Receptor (VDR) gene polymorphism and H. pylori infection. Materials and Methods: This cross-sectional study was conducted on 224 adult patients with upper gastrointestinal symptoms who underwent an upper gastrointestinal endoscopy between July 2017 and May 2019 in two major university hospitals. All patients were evaluated for helicobacter pylori infection. Two gastric antral biopsy specimens were taken from each patient. One of those Biopsy specimens was evaluated for histopathology examination and the other one was immersed in a saline solution ready for genomic DNA extraction. Results: There were statistically significant differences between different genotypes of VDR rs7975232 polymorphism between H. pylori infected and non-infected groups (CC was higher in H. pylori negative and AC and AA were the most common in H. pylori positive group). There were statistical differences between different genotypes of VDR rs2228570 between H. pylori infected and non-infected groups (TT was higher in H. pylori negative and CT and CC were the most common in H. pylori positive group). Regarding VDR rs 7975232 gene polymorphisms; the (A) allele was significantly higher H. pylori infected, while (C) allele was significantly higher in uninfected patients. Regarding VDR rs 2228570 gene polymorphisms; the (C) allele was significantly higher H. pylori infected, while (T) allele was significantly higher in uninfected patients. Conclusion: There is a possible association between the FokI and Apal VDR polymorphism and H. pylori infection.

scholarly journals Los genes como marcadores de riesgo en cáncer gástrico: revisión de estudios en población colombiana

Respuestas ◽  
2013 ◽  
Vol 18 (2) ◽  
pp. 61-73
Author(s):  
Claudia Marcela Yáñez-Gutiérrez
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Gene Polymorphisms ◽  
Genetic Factors ◽  
Human Gene ◽  
Risk Markers ◽  
Different Populations ◽  
H Pylori ◽  
Cáncer Gástrico

 El objetivo de esta revisión, fue identificar el rol de los genes como marcadores de riesgo en cáncer gástrico (CG) en población colombiana. Se revisaron publicaciones de investigaciones realizadas en los últimos diez años, utilizando las bases MEDLINE y LILACS y complementando  la pesquisa con la bibliografía relevante de los artículos. Se encontraron estudios en busca de asociación de CG con polimorfismos de varios genes humanos involucrados en la respuesta inmune, la desintoxicación y el supresor p53. En Colombia al igual que en otros países, las evidencias de asociación de polimorfismos genéticos con CG son aún controversiales, debido a la variación de los resultados que arrojan los estudios en las diferentes poblaciones. El genoma de las cepas de Helicobacter pylori que infectan población colombiana también ha sido investigado en búsqueda de polimorfismos de virulencia. El genotipo cagA/vacAs1m1 identificado como citotóxico en esta bacteria, mostró en la mayoría de las investigaciones, asociación con CG. La evidencia de asociación de CG con factores genéticos en población colombiana no es concluyente. Está lejos aún, la identificación de marcadores genéticos que permitan predecir el riesgo a desarrollar CG. A pesar de ello, algunos polimorfismos de genes humanos como los de IL-1 o los de algunas enzimas desintoxicantes, así como los genes cagA y vacA de Helicobacter pylori podrían ser candidatos a futuros marcadores de riesgo en esta neoplasia.Palabras clave: cáncer gástrico, riesgo, genotipo, Colombia. ABSTRACT  The objective of this review was to identify the role of genes as risk markers in gastric cancer (GC) in Colombian population studies. The study reviewed research publications in the last ten years, using the MEDLINE and LILACS, as well as various literature research of relevant articles. Searching studies found GC association with several human gene polymorphisms involved in the immune response, detoxification and suppressor p53. In Colombia, as in other countries, the evidence of the association of genetic polymorphisms with GC are still controversial because of the variation in results that studies in different populations. The genome of Helicobacter pylori strains that infect Colombian population has also been investigated in search of polymorphisms of virulence. cagA/ vacAs1m1 genotype identified as cytotoxic in this bacterium, demonstrated most of the research associated with GC. Evidence of association of GC with Colombian population genetic factors was inconclusive. It is yet to be determined the exact identification of genetic markers that can predict the risk of developing GC. However, some human gene polymorphisms as IL-1 or some detoxifying enzymes and the vacA and cagA of H. pylori could be candidates for future risk markers in these tumors.Keywords: gastric cancer, risk, genotype, Colombia

scholarly journals Cytokine TGF-β1, TNF-α, IFN-γ and IL‐6 Gene Polymorphisms and Localization of Premalignant Gastric Lesions in Immunohistochemically H. pylori-negative Patients

2021 ◽  
Vol 18 (12) ◽  
pp. 2743-2751
Author(s):  
Anca Negovan ◽  
Mihaela Iancu ◽  
Florin Tripon ◽  
Andrei Crauciuc ◽  
Simona Mocan ◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Gastric Lesions ◽  
Tnf Α ◽  
Ifn Γ ◽  
H Pylori ◽  
Tgf Β1

scholarly journals The Relationship between Toll-like Receptors andHelicobacter pylori-Related Gastropathies: Still a Controversial Topic

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Lorena Elena Meliț ◽  
Cristina Oana Mărginean ◽  
Cristian Dan Mărginean ◽  
Maria Oana Mărginean
Keyword(s):  
Gastric Cancer ◽  
Gene Polymorphisms ◽  
Experimental Studies ◽  
Large Family ◽  
Bacterial Invasion ◽  
Toll Like Receptors ◽  
Controversial Topic ◽  
H Pylori ◽  
Antigen Presenting

Innate immunity represents the first barrier against bacterial invasion. Toll-like receptors (TLRs) belong to the large family of pattern recognition receptors (PRRs), and their activation leads to the induction of inflammatory cytokines, chemokines, antigen-presenting molecules, and costimulatory molecules. Recent studies have focused on identifying the association between TLRs andHelicobacter pylori- (H. pylori-) related diseases. Therefore, this minireview focuses on assessing the role of these TLRs in the development ofH. pylori-related gastropathies. Both TLR2 and TLR were found to be involved inH. pyloriLPS recognition, with contradictory results most likely due to both the inability to obtain pure LPS in experimental studies and the heterogeneity of the bacterial LPS. In addition,TLR2was found to be the most extensively expressed gene among all the TLRs in gastric tumors. High levels of TLR4 were also associated with a higher risk of gastric cancer. TLR5 was initially associated with the recognition ofH. pyloriflagellin, but it seems that this bacterium has developed mechanisms to escape this recognition representing an important factor involved in the persistence of this infection and subsequent carcinogenesis. TLR9, the only TLR with both anti- and proinflammatory roles, was involved in the recognition ofH. pyloriDNA. The dichotomous role of TLR9, promoting or suppressing the infection, depends on the gastric environment. Recently, TLR7 and TLR8 were shown to recognize purifiedH. pyloriRNA, thereby inducing proinflammatory cytokines.TLR1andTLR10gene polymorphisms were associated with a higher risk for gastric cancer inH. pylori-infected individuals. Different gene polymorphisms of these TLRs were found to be associated with gastric cancer depending mostly on ethnicity. Further studies are required in order to develop preventive and therapeutic strategies againstH. pyloriinfections based on the functions of TLRs.

scholarly journals Association of Tumor Necrosis Factor Receptor 1 Promoter Gene Polymorphisms (-580 A/G and -609 G/T) and TNFR1 Serum Levels with the Susceptibility to Gastric Precancerous Lesions and Gastric Cancer Related to H. pylori Infection in a Moroccan Population

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ghizlane Bounder ◽  
Mohamed R. Jouimyi ◽  
Hasna Boura ◽  
Hassan Jouhadi ◽  
Wafaa Badre ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gene Polymorphisms ◽  
Precancerous Lesions ◽  
Precancerous Lesion ◽  
Tumor Necrosis Factor Receptor ◽  
Serum Levels ◽  
Recessive Model ◽  
Gastric Lesions ◽  
Promoter Gene ◽  
H Pylori

Chronic inflammation due to H. pylori infection is the risk factor of gastric cancer (GC). Through its receptor (TNFR1), TNF-α plays a fundamental role in inflammatory, infectious, and tumor processes. Dysregulation of TNFR1 gene expression could impact many biological processes that can lead to cancer. This study is aimed at evaluating the association of TNFR1 promoter gene polymorphisms (-580 A/G and -609 G/T) and TNFR1 serum levels with GC and precancerous lesion susceptibility. Patients suffering from gastric lesions (65 chronic gastritis, 50 precancerous lesions, and 40 GC) related to H. pylori infection and 63 healthy controls (HC) were involved in this study. Individuals are genotyped by TNFR1 gene promoter sequencing, and TNFR1 serum levels were measured by the ELISA quantitative method. Concerning TNFR1 -609 G/T locus, we noticed that the T allele was associated with an attenuated susceptibility to GC (OR=0.4; p value = 0.02). At the genotypic level and under the recessive model, the TNFR1 -609 TT genotype showed a decreased risk of GC (OR=0.3, p value = 0.03) compared to the combined (GG/GT) genotypes. TNFR1 serum levels have been increased together with gastric lesion severity (p value < 0.05). The TNFR1 -609 TT genotype seemed linked to a low level of sTNFR1 compared to GT and GG genotypes (p value = 0.07). Concerning TNFR1 -580 A/G locus, no significant relation was noticed between this polymorphism and GC susceptibility, as well as with the TNFR1 serum level. Our results suggest that the TNFR1 -609 T allele appears to have a protective effect against GC. High levels of TNFR1 serum levels seemed to be associated with the aggressiveness of gastric lesions. Therefore, our results suggest that TNFR1 -609 T/G polymorphism and the TNFR1 serum levels may be related to GC susceptibility.

scholarly journals Proinflammatory Cytokine IL-17 Shows a Significant Association with Helicobacter pylori Infection and Disease Severity

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Piyumali Sandareka Arachchi ◽  
Neluka Fernando ◽  
Manjula Manoji Weerasekera ◽  
Bimalka Senevirathna ◽  
Deepaka D. Weerasekera ◽  
...  
Keyword(s):  
Immune Response ◽  
Helicobacter Pylori ◽  
Disease Severity ◽  
Inflammatory Cytokines ◽  
Proinflammatory Cytokines ◽  
Proinflammatory Cytokine ◽  
Th17 Cells ◽  
Sri Lankan ◽  
Healthy Controls ◽  
H Pylori

Background. The pro- and anti-inflammatory cytokines play an important role in the immune response against H. pylori infection. The proinflammatory cytokines of Th17 cells have been suggested to play a major role in H. pylori infection and resulting gastric inflammation. Objective. The objective of this study was to compare the expression of selected inflammatory cytokines (IL-10, IL-17, IL-21, IL-23, and TNF-α) in H. pylori-infected patients and healthy controls and to understand their association with H. pylori infection and disease severity. Results. The expression levels of IL-17 and IL-23 were significantly higher in H. pylori-infected patients. The expression of IL-21 was also higher in H. pylori-positive patients but there was no significant association with infection. IL-17 expression showed a significant increase with the severity of chronic gastritis. Conclusion. The proinflammatory cytokine, IL-17, shows a significant association with H. pylori infection and disease severity in a Sri Lankan dyspeptic patient population.

Sign in / Sign up

Export Citation Format

Share Document