Endothelium-Dependent Relaxation Effects of Actinidia arguta Extracts in Coronary Artery: Involvement of eNOS/Akt Pathway

Cardiovascular diseases (CVD) are the major cause of death globally. Bioavailability of nitric oxide, antioxidative activity, and regulation of ionic homeostasis are the key targets for prevention of CVD. Actinidia arguta (AA) has shown promising effect for anticancer, anti-hypercholesterolemia, and antioxidant agents. However, the vascular effect of AA remains unclear. Therefore, we investigated the vascular relaxation of AA extract as well as the underlying mechanisms. Vascular reactivity was assessed in organ baths using porcine coronary arteries and antioxidant properties were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Methanol extract of AA stem (AASE) induced significantly vasorelaxation of porcine coronary artery and its effects is endothelium-dependent without cytotoxicity effects. In addition, ASSE scavenged reactive oxygen species (ROS) in vitro and strongly inhibited NADPH-oxidase activity, which is major source of ROS in vasculature. AASE strongly and dose-dependently activate endothelial nitric oxide synthase (eNOS), the major vascular protective enzyme, and Akt, the upstream signaling protein of eNOS, in porcine coronary artery endothelial cell. Altogether, these results have demonstrated that AASE is a potent endotheliumdependent vasodilator and this effect was involved in, at least in part, Akt/eNOS/NO pathway with strong anti-oxidant properties. The present findings indicate that AA stem could be a valuable candidate of herbal medicine for cardiovascular diseases associated with endothelial dysfunction and atherosclerosis.

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Endothelium-Dependent Vasorelaxant Effect of Butanolic Fraction fromCaryocar brasilienseCamb. Leaves in Rat Thoracic Aorta

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/934142 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Lais Moraes de Oliveira ◽

Aline Gabriela Rodrigues ◽

Elaine Fernanda da Silva ◽

Letícia Bonancio Cerqueira ◽

Carlos Henrique Castro ◽

...

Keyword(s):

Nitric Oxide ◽

Dependent Manner ◽

Native Plant ◽

Concentration Dependent Manner ◽

Vascular Effect ◽

Nitric Oxide Synthase Inhibitor ◽

Vasorelaxant Effect ◽

Antioxidant Agents

Caryocar brasilienseCamb. “pequi” is a native plant from the Cerrado region of Brazil that contains bioactive components reported to be antioxidant agents. Previous work has demonstrated that dietary supplementation with pequi decreased the arterial pressure of volunteer athletes. We found that the crude hydroalcoholic extract (CHE) ofC. brasilienseleaves relaxed, in a concentration-dependent manner, rat aortic rings precontracted with phenylephrine, and that the butanolic fraction (BF) produced an effect similar to that of the CHE. Aortic relaxation induced by BF was abolished by endothelium removal, by incubation of the nitric oxide synthase inhibitor L-NAME, or the soluble guanylatecyclase inhibitor ODQ. However, incubation with atropine and pyrilamine had no effect on the BF-induced vasorelaxation. Moreover, this effect was not inhibited by indomethacin and tetraethylammonium. The concentration-response curve to calcium in denuded-endothelium rings was not modified after incubation with BF, and the vasorelaxation by BF in endothelium-intact rings precontracted with KCl was abolished after incubation with L-NAME. In addition, administration of BF in anesthetized rats resulted in a reversible hypotension. The results reveal thatC. brasiliensepossesses both in vivo and in vitro activities and that the vascular effect of BF involves stimulation of the nitric oxide/cyclic GMP pathway.

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Synthesis of Novel Nitric Oxide Releasing Furoxan Hybrids from Biologically Active Bicyclic Amine and their Antioxidant Activity

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.21948 ◽

2019 ◽

Vol 31 (8) ◽

pp. 1724-1728

Author(s):

P.M. Swami ◽

P.K. Zubaidha ◽

G.B. Tiwari

Keyword(s):

Nitric Oxide ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Biologically Active ◽

Scavenging Effect ◽

Antioxidant Agents ◽

Radical Scavenging Effect ◽

Nitric Oxide Releasing

The present paper describes the synthesis of novel nitric oxide hybrids obtained by linking bioactive bicyclic amine to substituted furoxans. The antioxidant activities were studied in vitro based on the radical scavenging effect of stable DPPH free radical using ascorbic acid as a standard. The nitric oxide hybrids showed remarkable antioxidant properties and hence, can be employed as potential antioxidant agents.

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Role of nitric oxide in adenosine receptor-mediated relaxation of porcine coronary artery

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1995.269.5.h1672 ◽

1995 ◽

Vol 269 (5) ◽

pp. H1672-H1678 ◽

Cited By ~ 15

Author(s):

W. Abebe ◽

T. Hussain ◽

H. Olanrewaju ◽

S. J. Mustafa

Keyword(s):

Nitric Oxide ◽

Coronary Artery ◽

Porcine Coronary Artery ◽

Cyclic Monophosphate ◽

Enhanced Production ◽

Cgs 21680 ◽

Synthase Inhibitor ◽

Ly 83583

In the present study, using porcine coronary artery rings in vitro, we examined the role of the nitric oxide (NO) pathway in endothelium-dependent vasorelaxant effects of the 5'-uronamide adenosine agonists, 5'-(N-ethylcarboxamido)adenosine (NECA) and 2-[p-(2-carboxyethyl)]phenylethyl-amino-5'-N-ethylcarboxamidoadenosine (CGS-21680) as opposed to the endothelium-independent actions of the C2- and N6-substituted analogues, 2-chloroadenosine (CAD) and N6-cyclopentyladenosine (CPA). The NO synthase inhibitor, NG-monomethyl-L-arginine (L-NMMA, 30 microM), and the NO-destroying agent, 6-anilino-5,8-quinolinedione (LY-83583, 10 microM), attenuated the relaxations of endothelium-intact but not -denuded rings to NECA and CGS-21680. The effect of L-NMMA on NECA-induced relaxation was reversed by L-arginine (100 microM), a substrate for NO synthesis. In the endothelium-intact tissues, both NECA and CGS-21680 elicited enhanced production of nitrite, a stable metabolite of NO. This was also attenuated by L-NMMA or endothelium removal. Furthermore, NECA (10 microM) induced augmentation of guanosine 3',5'-cyclic monophosphate (cGMP) production in the intact arteries, which was also inhibited by L-NMMA, LY-83583, or endothelium removal. In contrast, vasorelaxant responses generated by CAD and CPA were not altered by either L-NMMA or LY-83583. Both agents (10 microM) were also unable to alter nitrite and/or guanosine 3',5'-cyclic monophosphate (cGMP) levels of the coronary artery.(ABSTRACT TRUNCATED AT 250 WORDS)

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Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery

Plants ◽

10.3390/plants9121688 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1688

Author(s):

Deepak Adhikari ◽

Dal-Seong Gong ◽

Se Hee Oh ◽

Eun Hee Sung ◽

Seung On Lee ◽

...

Keyword(s):

Coronary Artery ◽

Cardiovascular Diseases ◽

Herbal Medicine ◽

Vascular Reactivity ◽

Ex Vivo ◽

Vascular Diseases ◽

Developed Countries ◽

Porcine Coronary Artery ◽

Vasorelaxant Effect ◽

Boesenbergia Rotunda

Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes from Boesenbergia rotunda (L.) Mansf. [Boesenbergia pandurata (Roxb.) Schltr.] used as a spice and herbal medicine in Asian countries. The methanol extract of B. rotunda rhizomes (BRE) exhibited significant vasorelaxation effects ex vivo at EC50 values of 13.4 ± 6.1 μg/mL and 40.9 ± 7.9 μg/mL, respectively, with and without endothelium in the porcine coronary artery ring. The intrinsic mechanism was evaluated by treating with specific inhibitors or activators that typically affect vascular reactivity. The results suggested that BRE induced relaxation in the coronary artery rings via an endothelium-dependent pathway involving NO-cGMP, and also via an endothelium-independent pathway involving the blockade of Ca2+ channels. Vasorelaxant principles in BRE were identified by subsequent chromatographic methods, which revealed that flavonoids regulate vasorelaxant activity in BRE. One of the flavonoids was a Diels-Alder type adduct, 4-hydroxypanduratin A, which showed the most potent vasorelaxant effect on porcine coronary artery with an EC50 of 17.8 ± 2.5 μM. Our results suggest that rhizomes of B. rotunda might be of interest as herbal medicine against cardiovascular diseases.

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Antioxidant Properties of S-Nitrosoglutathione and Nanotechnologies

Proceedings ◽

10.3390/proceedings2019011015 ◽

2019 ◽

Vol 11 (1) ◽

pp. 15

Author(s):

Parent ◽

Zhou ◽

Bonetti ◽

Perrin-Sarrado ◽

Lartaud ◽

...

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Cardiovascular Diseases ◽

Sustained Release ◽

Antioxidant Properties ◽

Protein S ◽

Post Translational Modification ◽

Antioxidant Power

Cardiovascular diseases are associated with oxidative stress and a reduced bioavailability of nitric oxide (NO). To counteract both processes, the administration of S-nitrosoglutathione (GSNO) can be envisaged. GSNO is able to induce protein S-nitrosation (Pr-SNO), which is a post-translational modification of proteins, participating in the storage of NO in tissues, and protect thiol functions from oxidation. However, GSNO antioxidant power is poorly studied, which is probably linked to its low stability. This low stability can be addressed by nanotechnologies that will increase GSNO protection and provide a sustained release of the drug.

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Adenosine Derivates as Antioxidant Agents: Synthesis, Characterization, in Vitro Activity, and Theoretical Insights

Antioxidants ◽

10.3390/antiox8100468 ◽

2019 ◽

Vol 8 (10) ◽

pp. 468 ◽

Cited By ~ 1

Author(s):

Francisco Valdes ◽

Nelson Brown ◽

Alejandro Morales-Bayuelo ◽

Luis Prent-Peñaloza ◽

Margarita Gutierrez

Keyword(s):

Antioxidant Properties ◽

Free Radical Scavengers ◽

Pharmacokinetic Parameters ◽

Quantum Similarity ◽

Antioxidant Agents ◽

Molecular Quantum Similarity ◽

Adenosine Derivatives ◽

Oxidative Processes ◽

Theoretical Analyses

In this work, we present results about the synthesis and the antioxidant properties of seven adenosine derivatives. Four of these compounds were synthesized by substituting the N6-position of adenosine with aliphatic amines, and three were obtained by modification of the ribose ring. All compounds were obtained in pure form using column chromatography, and their structures were elucidated by infrared spectroscopy (IR) and Nuclear Magnetic Resonance (NMR). All adenosine derivatives were further evaluated in vitro as free radical scavengers. Our results show that compounds 1c, 3, and 5 display a potent antioxidant effect compared with the reference compound ascorbic acid. In addition, the absorption, distribution, metabolism and excretion (ADME) calculations show favorable pharmacokinetic parameters for the set of compounds analyzed, which guarantees their suitability as potential antioxidant drugs. Furthermore, theoretical analyses using Molecular Quantum Similarity and reactivity indices were performed in order to discriminate the different reactive sites involved in oxidative processes.

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The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

Molecules ◽

10.3390/molecules24244570 ◽

2019 ◽

Vol 24 (24) ◽

pp. 4570 ◽

Cited By ~ 4

Author(s):

Katarína Kozics ◽

Mária Bučková ◽

Andrea Puškárová ◽

Viktória Kalászová ◽

Terézia Cabicarová ◽

...

Keyword(s):

Essential Oils ◽

Antioxidant Properties ◽

Total Antioxidant Status ◽

Human Keratinocytes ◽

Drug Resistant ◽

Healthy Human ◽

Antioxidant Agents ◽

Total Antioxidant ◽

Antibacterial And Antifungal

In this study, we determined the antimicrobial activity of ten essential oils (EOs)—oregano, thyme, clove, arborvitae, cassia, lemongrass, melaleuca, eucalyptus, lavender, and clary sage—against drug-resistant microorganisms previously isolated from patients with skin infections. The essential oil compositions were determined using gas chromatography coupled to mass spectrometry (GC/MS). The assayed bacteria included Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter koseri, and Klebsiella pneumoniae. Two drug-resistant yeasts (Candida albicans and Candida parapsilosis) were also involved in our survey. Oregano, thyme, cassia, lemongrass and arborvitae showed very strong antibacterial and antifungal activity against all tested strains. These results show that these essential oils may be effective in preventing the growth of the drug-resistant microorganisms responsible for wound infections. In this study, the genotoxic effects of tested essential oils on healthy human keratinocytes HaCaT were evaluated using the comet assay for the first time. These results revealed that none of the essential oils induced significant DNA damage in vitro after 24 h. Moreover, the treatment of HaCaT cells with essential oils increased the total antioxidant status (TAS) level. The obtained results indicate that EOs could be used as a potential source of safe and potent natural antimicrobial and antioxidant agents in the pharmaceutical and food industries.

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Two mechanisms mediate relaxation by bradykinin of pig coronary artery: NO-dependent and -independent responses

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1991.261.3.h830 ◽

1991 ◽

Vol 261 (3) ◽

pp. H830-H835 ◽

Cited By ~ 13

Author(s):

C. L. Cowan ◽

R. A. Cohen

Keyword(s):

Nitric Oxide ◽

Methylene Blue ◽

Coronary Artery ◽

Thromboxane A2 ◽

Porcine Coronary Artery ◽

Cyclic Monophosphate ◽

Pig Coronary Artery ◽

Endothelium Dependent Relaxation

The role of nitric oxide and guanosine 3',5'-cyclic monophosphate (cGMP) accumulation in the endothelium-dependent relaxation of the porcine coronary artery to bradykinin was investigated by comparing relaxation and cGMP accumulation in the presence or absence of NG-monomethyl-L-arginine (L-NMMA) and methylene blue. Rings were treated with indomethacin to eliminate the effects of prostaglandins. Relaxation to bradykinin of rings contracted with the thromboxane A2 mimetic U-46619 was not affected by L-NMMA and was only minimally inhibited by methylene blue. Rings contracted with elevated potassium (25 mM) also relaxed completely to bradykinin. However, L-NMMA or methylene blue effectively inhibited relaxation to bradykinin in rings contracted with potassium. cGMP accumulation was stimulated by bradykinin and inhibited by L-NMMA or methylene blue in rings contracted with either U-46619 or potassium. These results suggest that in the absence of nitric oxide-induced cGMP accumulation, a nonprostanoid mechanism exists that is capable of completely relaxing U-46619-contracted coronary artery. This mechanism is either inhibited in or unable to relax potassium-contracted rings. These results also demonstrate that nitric oxide mediates the bradykinin-induced cGMP accumulation that is largely responsible for the relaxation during contraction with potassium.

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