scholarly journals The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4570 ◽  
Author(s):  
Katarína Kozics ◽  
Mária Bučková ◽  
Andrea Puškárová ◽  
Viktória Kalászová ◽  
Terézia Cabicarová ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Properties ◽  
Total Antioxidant Status ◽  
Human Keratinocytes ◽  
Drug Resistant ◽  
Healthy Human ◽  
Antioxidant Agents ◽  
Total Antioxidant ◽  
Antibacterial And Antifungal

In this study, we determined the antimicrobial activity of ten essential oils (EOs)—oregano, thyme, clove, arborvitae, cassia, lemongrass, melaleuca, eucalyptus, lavender, and clary sage—against drug-resistant microorganisms previously isolated from patients with skin infections. The essential oil compositions were determined using gas chromatography coupled to mass spectrometry (GC/MS). The assayed bacteria included Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter koseri, and Klebsiella pneumoniae. Two drug-resistant yeasts (Candida albicans and Candida parapsilosis) were also involved in our survey. Oregano, thyme, cassia, lemongrass and arborvitae showed very strong antibacterial and antifungal activity against all tested strains. These results show that these essential oils may be effective in preventing the growth of the drug-resistant microorganisms responsible for wound infections. In this study, the genotoxic effects of tested essential oils on healthy human keratinocytes HaCaT were evaluated using the comet assay for the first time. These results revealed that none of the essential oils induced significant DNA damage in vitro after 24 h. Moreover, the treatment of HaCaT cells with essential oils increased the total antioxidant status (TAS) level. The obtained results indicate that EOs could be used as a potential source of safe and potent natural antimicrobial and antioxidant agents in the pharmaceutical and food industries.

scholarly journals Disease-Dependent Antiapoptotic Effects of Cannabidiol for Keratinocytes Observed upon UV Irradiation

2021 ◽  
Vol 22 (18) ◽  
pp. 9956
Author(s):  
Piotr Wójcik ◽  
Agnieszka Gęgotek ◽  
Neven Žarković ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Signaling Pathways ◽  
Antioxidant Properties ◽  
Human Keratinocytes ◽  
Caspase 8 ◽  
Apoptotic Pathway ◽  
Healthy Human ◽  
Uvb Irradiation ◽  
Apoptotic Signaling ◽  
Anti Inflammatory

Although apoptosis of keratinocytes has been relatively well studied, there is a lack of information comparing potentially proapoptotic treatments for healthy and diseased skin cells. Psoriasis is a chronic autoimmune-mediated skin disease manifested by patches of hyperproliferative keratinocytes that do not undergo apoptosis. UVB phototherapy is commonly used to treat psoriasis, although this has undesirable side effects, and is often combined with anti-inflammatory compounds. The aim of this study was to analyze if cannabidiol (CBD), a phytocannabinoid that has anti-inflammatory and antioxidant properties, may modify the proapoptotic effects of UVB irradiation in vitro by influencing apoptotic signaling pathways in donor psoriatic and healthy human keratinocytes obtained from the skin of five volunteers in each group. While CBD alone did not have any major effects on keratinocytes, the UVB treatment activated the extrinsic apoptotic pathway, with enhanced caspase 8 expression in both healthy and psoriatic keratinocytes. However, endoplasmic reticulum (ER) stress, characterized by increased expression of caspase 2, was observed in psoriatic cells after UVB irradiation. Furthermore, decreased p-AKT expression combined with increased 15-d-PGJ2 level and p-p38 expression was observed in psoriatic keratinocytes, which may promote both apoptosis and necrosis. Application of CBD partially attenuated these effects of UVB irradiation both in healthy and psoriatic keratinocytes, reducing the levels of 15-d-PGJ2, p-p38 and caspase 8 while increasing Bcl2 expression. However, CBD increased p-AKT only in UVB-treated healthy cells. Therefore, the reduction of apoptotic signaling pathways by CBD, observed mainly in healthy keratinocytes, suggests the need for further research into the possible beneficial effects of CBD.

In vitro Assessment of the Antibacterial and Antioxidant Properties of Essential Oils

2019 ◽  
Vol 15 (5) ◽  
pp. 592-599 ◽  
Author(s):  
Danianni M. Zardo ◽  
Leydi V.H. Alvarez ◽  
Francine G.B. Los ◽  
Vivian C. Ito ◽  
Ana P. Travalini ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Mentha Piperita ◽  
Economic Losses ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

Background: Outbreaks of foodborne diseases cause substantial economic losses. Universities, research institutes and the food industry are increasing their efforts to enhance food safety worldwide. In this context, the study of essential oils as natural antimicrobials and antioxidants for use in foods has become increasingly important. Methods: The volatile composition and antioxidant and antibacterial activity of Pelargonium graveolens, Cymbopogon citratus, Citrus bergamia, Rosmarinus officinalis and Mentha piperita essential oils were evaluated. Results: The essential oils showed 80.5 to 95.0% of monoterpenes, but with different profiles. The free radical scavenging activity by the DPPH assay ranged from 3.53 to 68.55% and the total antioxidant potential measured by FRAP ranged from 1.28 to 94.61 mmol TE g-1. The antioxidant activity (FRAP and DPPH assays) of the essential oils followed the order: C. citratus > M. piperita > P. graveolens > C. bergamia > R. officinalis. In general, when the pH tended to neutrality a higher concentration of essential oil was needed to inactivate the microorganisms. The C. citratus oil presented interesting results regarding the inhibition of both strains of S. aureus, showing a lower MIC50 value and Minimum Bactericidal Concentration (MBC) in the concentrations evaluated for E. coli and S. enterica. Conclusion: The results suggest that C. citratus essential oil has potential as an antibacterial and antioxidant agent.

scholarly journals Chemical constituents, in vitro antibacterial and antifungal activity of Mentha×Piperita L. (peppermint) essential oils

2019 ◽  
Vol 31 (4) ◽  
pp. 528-533 ◽  
Author(s):  
Nagarjuna Reddy Desam ◽  
Abdul Jabbar Al-Rajab ◽  
Mukul Sharma ◽  
Mary Moses Mylabathula ◽  
Ramachandra Reddy Gowkanapalli ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Mentha Piperita ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

scholarly journals The Effect of Essential Oil Intake on Changes of Plasma Antioxidant Status in Mice

2007 ◽  
Vol 76 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Š. Faix ◽  
Š. Juhas ◽  
Z. Faixová
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
The Body ◽  
Thymus Vulgaris ◽  
High Dose ◽  
Syzygium Aromaticum ◽  
Lower Growth ◽  
Total Antioxidant

The aim of this study was to determine the effects of four essential oils intake by feed, namely Origanum vulgare, Thymus vulgaris, Cinnamomum zeylanicum Ness, and Syzygium aromaticum on antioxidant status in mice in vivo. Essential oils were in the aether oleum form. They were diluted with ethanol absolute mixed with ground pelett (0.1, 0.25, 0.57 and 1% concentration) and thereafter ethanol was evaporated. SOD, GPx activities and TAS were measured in erythrocytes and plasma spectrophotometrically with Ransod, Ransel and TAS kits from RANDOX, respectively. GPX activity showed a significant increase in 0.25% and 0.1% concentration of Origani aetheroleum. The GPx activities were decreased in 1% concentration of Thymi aetheroleum and 0.57% concentration of Cinnamomi aetheroleum and 0.57% concentration of Caryophylli aetheroleum. The total antioxidant status showed a significant decrease in 1 % concentration of Origani aetheroleum and significantly increased in 0.1% concentration. The same results were found in Thymi aetheroleum. Cinnamomi aetheroleum and Caryophylli aetheroleum had not effect on total antioxidant status. SOD activities were not significantly changed after intake of essential oils. In conclusion, our results showed, that concentration of essential oil is very important for antioxidant status and also for metabolism of mice, because a high dose of essential oil has adverse effect on metabolism of mice, representated by a lower growth of the body weight. On the other hand, essential oils at lower concentrations have positive effect on antioxidant status of mice.

scholarly journals Comparative Evaluation of Essential Oils from Medicinal-Aromatic Plants of Greece: Chemical Composition, Antioxidant Capacity and Antimicrobial Activity against Bacterial Fish Pathogens

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 148 ◽  
Author(s):  
Thekla I. Anastasiou ◽  
Manolis Mandalakis ◽  
Nikos Krigas ◽  
Thomas Vézignol ◽  
Diamanto Lazari ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
In Vitro Assays ◽  
Aromatic Plants ◽  
Farmed Fish ◽  
Fish Pathogens ◽  
Antioxidant Agents ◽  
The Impact

The administration of antibiotics in aquaculture has raised concern about the impact of their overuse in marine ecosystems, seafood safety and consumers’ health. This “green consumerism” has forced researchers to find new alternatives against fish pathogens. The present study focused on 12 Mediterranean medicinal-aromatic plants as potential antimicrobials and antioxidant agents that could be used in fish aquaculture. In vitro assays showed that the essential oils (EOs) from all studied plants had anti-bacterial and antioxidant properties, with their efficacy being dependent on their chemical composition. More specifically, EOs rich in carvacrol, p-cymene and γ-terpinene exhibited not only the strongest inhibitory activity against the growth of bacterial pathogens (inhibitory concentration: 26–88 μg mL−1), but also the greatest total antioxidant capacity (ABTS: 2591–5879 μmole mL−1; CUPRAC: 931–2733 μmole mL−1). These compounds were mainly found in the EOs from Greek oregano (Origanum vulgare subsp. hirtum), Spanish oregano (Thymbra capitata) and savoury (Satureja thymbra) collected from cultivations in Greece. The specific EOs stand out as promising candidates for the treatment of bacterial diseases and oxidative stress in farmed fish. Further in vivo experiments are needed to fully understand the effects of EO dietary supplementation on fish farming processes.

scholarly journals Adenosine Derivates as Antioxidant Agents: Synthesis, Characterization, in Vitro Activity, and Theoretical Insights

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 468 ◽  
Author(s):  
Francisco Valdes ◽  
Nelson Brown ◽  
Alejandro Morales-Bayuelo ◽  
Luis Prent-Peñaloza ◽  
Margarita Gutierrez
Keyword(s):  
Antioxidant Properties ◽  
Free Radical Scavengers ◽  
Pharmacokinetic Parameters ◽  
Quantum Similarity ◽  
Antioxidant Agents ◽  
Molecular Quantum Similarity ◽  
Adenosine Derivatives ◽  
Oxidative Processes ◽  
Theoretical Analyses

In this work, we present results about the synthesis and the antioxidant properties of seven adenosine derivatives. Four of these compounds were synthesized by substituting the N6-position of adenosine with aliphatic amines, and three were obtained by modification of the ribose ring. All compounds were obtained in pure form using column chromatography, and their structures were elucidated by infrared spectroscopy (IR) and Nuclear Magnetic Resonance (NMR). All adenosine derivatives were further evaluated in vitro as free radical scavengers. Our results show that compounds 1c, 3, and 5 display a potent antioxidant effect compared with the reference compound ascorbic acid. In addition, the absorption, distribution, metabolism and excretion (ADME) calculations show favorable pharmacokinetic parameters for the set of compounds analyzed, which guarantees their suitability as potential antioxidant drugs. Furthermore, theoretical analyses using Molecular Quantum Similarity and reactivity indices were performed in order to discriminate the different reactive sites involved in oxidative processes.

scholarly journals Potential Application of Prunus armeniaca L. and P. domestica L. Leaf Essential Oils as Antioxidant and of Cholinesterases Inhibitors

Antioxidants ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 2 ◽  
Author(s):  
Marco Bonesi ◽  
Maria Tenuta ◽  
Monica Loizzo ◽  
Vincenzo Sicari ◽  
Rosa Tundis
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Positive Control ◽  
Prunus Armeniaca ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Ic50 Values ◽  
Comprehensive Comparison

The aim of this work is to investigate the in vitro acetylcholinesterase (AChE) and butyrycholinesterase (BChE) inhibitory activities of essential oils obtained by hydrodistillation from the leaves of Prunus armeniaca and P. domestica in relation to their composition, analysed by Gas Chromatography–Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses, at different times. Moreover, considering the role of free radicals in the progression of neurodegenerative disorders, the antioxidant properties of essential oils were investigated by using, 2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and β-carotene bleaching tests. The relative antioxidant capacity index (RACI) was used to achieve more comprehensive comparison between analysed antioxidant effects of essential oils. P. armeniaca oils were more active than P. domestica oils against AChE. Against BChE, the most active was the essential oil from P. domestica leaves collected in August with an IC50 value of 95.80 μg/mL. This oil exerted the highest inhibitory activity of lipid peroxidation with IC50 values of 11.15 and 11.39 μg/mL after 30 and 60 min of incubation, respectively. All samples demonstrated a remarkable ABTS radicals scavenging activity, with IC50 values in the range 0.45–0.57 μg/mL in comparison to the positive control, ascorbic acid.

Non-Phenolic Antioxidant Compounds from Buddleja asiatica

2008 ◽  
Vol 63 (7-8) ◽  
pp. 483-491 ◽  
Author(s):  
Mortada M. El-Sayed ◽  
El-Sayed S. Abdel-Hameed ◽  
Wafaa S. Ahmed ◽  
Eman A. El-Wakil
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Chemical Analyses ◽  
Dpph Assay ◽  
Antioxidant Agents ◽  
Total Antioxidant

The methanol extract of the leaves of Buddleja asiatica Lour. (Loganiaceae) showed antioxidant activity toward the well known in vitro antioxidant tests such as total antioxidant capacity by the phosphomolybdenum method, free radical scavenging activity by the 1,1- diphenyl-2-picrylhydrazyl scavenging assay (DPPH assay) and hydrogen peroxide scavenging methods. Due to the high scavenging activity of the n-butanol successive fraction toward DPPH and H2O2 (SC50 = 11.99 and 18.54 μg/ml, respectively), this extract was subjected to chromatographic separation and isolation. Four non-phenolic compounds were isolated and identified on the basis of spectroscopic and chemical analyses: 1-O-ß-D-glucopyranosyl- 2-methoxy-3-(2-hydroxy-triaconta-3,12-dienoate)-glycerol (1), 3-O-[α-l-rhamnopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-glucopyranosyl-(1→2)]-ß-d-fucopyranosyl-olean- 11,13(18)-diene-3ß,23,28-triol (2), 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-ß-d-fucopyranosyl-olean-11,13(18)-diene-3ß,23,28-triol (3), and 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-xylopyranosyl- (1→2)]-ß-d-glucuronopyranosyl-acid-olean-11,13(18)-diene-3ß,23,28-triol (4). The four compounds were evaluated as antioxidant agents using the three antioxidant bioassay tests.

The Effect of Germination on Antioxidant and Nutritional Parameters of Protein Isolates from Grass Pea (Lathyrus sativus) Seeds

2010 ◽  
Vol 16 (1) ◽  
pp. 73-77 ◽  
Author(s):  
A. Starzyńska-Janiszewska ◽  
B. Stodolak ◽  
B. Mickowska
Keyword(s):  
Antioxidant Properties ◽  
Sulfur Amino Acids ◽  
Grass Pea ◽  
Protein Isolates ◽  
Nutritional Parameters ◽  
Pea Seedlings ◽  
Total Antioxidant ◽  
Pea Seeds ◽  
Potential Food

The aim of this research was to study the antioxidant and nutritional (selected objects) properties of protein isolates obtained from grass pea seedlings as compared with soaked and raw seeds. Two percent extract of isolate from 5-day-old seedlings showed the highest total antioxidant activity (25%) and the ability to chelate Fe 2+ (2.35 mg/g d.m.) as compared with other isolates. Protein isolates from grass pea seeds had on average 89% total protein, 87% in vitro protein bioavailability, about 5574 TIU/g (d.m.) (trypsin inhibitors activity) and did not contain ODAP. Germination of seeds for 5 days considerably improved the in vitro bioavailability of isolates, by 12%, and profile of sulfur amino acids by 42%, in comparison with isolates obtained from the raw seeds. Isolates from 5-day-old grass pea seedlings had the best antioxidant properties and improved nutritional parameters (as compared with raw seeds), which makes them worthy of being considered as a potential food additive.

Sign in / Sign up

Export Citation Format

Share Document