Evaluation of Minimally Invasive Arthroscopy Technology in Sports Medicine—An Evaluation of Nano Silver Disinfectants

2021 ◽  
Vol 21 (3) ◽  
pp. 1446-1450
Author(s):  
Ruiyi Ge ◽  
Jin Zhang ◽  
Ziquan Yang

Currently, nano silver fungicide prepared in the laboratory is used to disinfect arthroscopic surgical instruments. In this study, nano silver fungicides with stable properties were prepared and characterized. Afterwards, their bactericidal properties as well as mucus peeling properties were further tested. The results show that the nano silver fungicide prepared here contains uniform particle size and displays material stability for 60 days. Nano silver fungicide can completely kill sulfate-reducing bacteria, anaerobic bacteria, and iron reducing bacteria, while the slime stripping rate is 80.58%. Additionally, we propose the use of nano silver sterilization agents to kill the arthroscopic surgical instruments in conjunction with proper manual cleaning, as they can effectively kill all the bacteria on the surgical instruments, achieving a sterilization rate of 99.99%.

Author(s):  
Teyyub İsmayılov, Sevinj Suleymanova Teyyub İsmayılov, Sevinj Suleymanova

Complex compounds were synthesized by mixing natural petroleum acids with hexamethylene diamine at a ratio of 1:1 and 2:1 at room temperature, IR spectra of the complexes were studied and confirmed, physical and chemical parameters were determined, solutions were prepared and bactericidal properties were studied. The bactericidal effect of a complex compound of natural petroleum acids synthesized with hexamethylenediamine in a ratio of 1: 1 mol at a concentration of 250 mg / l is 95%, a complex combination obtained at a concentration of 500 mg / l and 1000 mg / l is 100%, bactericidal at a concentration of 2: 1 mol at a concentration of 250 mg / l The effect was 98%, 100% at 500 and 1000 mg / l concentrations. According to the results, complex compounds of natural petroleum acids synthesized with hexamethylenediamine can be used to prevent the growth of bacteria. Keywords: natural petroleum acid, hexamethylenediamine, sulfate-reducing bacteria, complex compounds, bactericidal properties.


2016 ◽  
Vol 26 (1-3) ◽  
pp. 152-164 ◽  
Author(s):  
Johann Heider ◽  
Karola Schühle ◽  
Jasmin Frey ◽  
Bernhard Schink

Acetone and other ketones are activated for subsequent degradation through carboxylation by many nitrate-reducing, phototrophic, and obligately aerobic bacteria. Acetone carboxylation leads to acetoacetate, which is subsequently activated to a thioester and degraded via thiolysis. Two different types of acetone carboxylases have been described, which require either 2 or 4 ATP equivalents as an energy supply for the carboxylation reaction. Both enzymes appear to combine acetone enolphosphate with carbonic phosphate to form acetoacetate. A similar but more complex enzyme is known to carboxylate the aromatic ketone acetophenone, a metabolic intermediate in anaerobic ethylbenzene metabolism in denitrifying bacteria, with simultaneous hydrolysis of 2 ATP to 2 ADP. Obligately anaerobic sulfate-reducing bacteria activate acetone to a four-carbon compound as well, but via a different process than bicarbonate- or CO<sub>2</sub>-dependent carboxylation. The present evidence indicates that either carbon monoxide or a formyl residue is used as a cosubstrate, and that the overall ATP expenditure of this pathway is substantially lower than in the known acetone carboxylase reactions.


Minerals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 413 ◽  
Author(s):  
Fan Xu ◽  
Xuelian You ◽  
Qing Li ◽  
Yi Liu

Microbes can mediate the precipitation of primary dolomite under surface conditions. Meanwhile, primary dolomite mediated by microbes often contains more Fe2+ than standard dolomite in modern microbial culture experiments. Ferroan dolomite and ankerite have been regarded as secondary products. This paper reviews the process and possible mechanisms of microbial mediated precipitation of primary ferroan dolomite and/or ankerite. In the microbial geochemical Fe cycle, many dissimilatory iron-reducing bacteria (DIRB), sulfate-reducing bacteria (SRB), and methanogens can reduce Fe3+ to Fe2+, while SRB and methanogens can also promote the precipitation of primary dolomite. There are an oxygen respiration zone (ORZ), an iron reduction zone (IRZ), a sulfate reduction zone (SRZ), and a methanogenesis zone (MZ) from top to bottom in the muddy sediment diagenesis zone. DIRB in IRZ provide the lower section with Fe2+, which composes many enzymes and proteins to participate in metabolic processes of SRB and methanogens. Lastly, heterogeneous nucleation of ferroan dolomite on extracellular polymeric substances (EPS) and cell surfaces is mediated by SRB and methanogens. Exploring the origin of microbial ferroan dolomite may help to solve the “dolomite problem”.


2020 ◽  
Vol 8 (9) ◽  
pp. 1375
Author(s):  
Ana J. Cavaleiro ◽  
Ana P. Guedes ◽  
Sérgio A. Silva ◽  
Ana L. Arantes ◽  
João C. Sequeira ◽  
...  

Long-chain fatty acids (LCFA) are common contaminants in municipal and industrial wastewater that can be converted anaerobically to methane. A low hydrogen partial pressure is required for LCFA degradation by anaerobic bacteria, requiring the establishment of syntrophic relationships with hydrogenotrophic methanogens. However, high LCFA loads can inhibit methanogens, hindering biodegradation. Because it has been suggested that anaerobic degradation of these compounds may be enhanced by the presence of alternative electron acceptors, such as iron, we investigated the effect of sub-stoichiometric amounts of Fe(III) on oleate (C18:1 LCFA) degradation by suspended and granular methanogenic sludge. Fe(III) accelerated oleate biodegradation and hydrogenotrophic methanogenesis in the assays with suspended sludge, with H2-consuming methanogens coexisting with iron-reducing bacteria. On the other hand, acetoclastic methanogenesis was delayed by Fe(III). These effects were less evident with granular sludge, possibly due to its higher initial methanogenic activity relative to suspended sludge. Enrichments with close-to-stoichiometric amounts of Fe(III) resulted in a microbial community mainly composed of Geobacter, Syntrophomonas, and Methanobacterium genera, with relative abundances of 83–89%, 3–6%, and 0.2–10%, respectively. In these enrichments, oleate was biodegraded to acetate and coupled to iron-reduction and methane production, revealing novel microbial interactions between syntrophic LCFA-degrading bacteria, iron-reducing bacteria, and methanogens.


2006 ◽  
Vol 72 (7) ◽  
pp. 4672-4687 ◽  
Author(s):  
Darrell P. Chandler ◽  
Ann E. Jarrell ◽  
Eric R. Roden ◽  
Julia Golova ◽  
Boris Chernov ◽  
...  

ABSTRACT A 16S rRNA-targeted tunable bead array was developed and used in a retrospective analysis of metal- and sulfate-reducing bacteria in contaminated subsurface sediments undergoing in situ U(VI) bioremediation. Total RNA was extracted from subsurface sediments and interrogated directly, without a PCR step. Bead array validation studies with total RNA derived from 24 isolates indicated that the behavior and response of the 16S rRNA-targeted oligonucleotide probes could not be predicted based on the primary nucleic acid sequence. Likewise, signal intensity (absolute or normalized) could not be used to assess the abundance of one organism (or rRNA) relative to the abundance of another organism (or rRNA). Nevertheless, the microbial community structure and dynamics through time and space and as measured by the rRNA-targeted bead array were consistent with previous data acquired at the site, where indigenous sulfate- and iron-reducing bacteria and near neighbors of Desulfotomaculum were the organisms that were most responsive to a change in injected acetate concentrations. Bead array data were best interpreted by analyzing the relative changes in the probe responses for spatially and temporally related samples and by considering only the response of one probe to itself in relation to a background (reference) environmental sample. By limiting the interpretation of the data in this manner and placing it in the context of supporting geochemical and microbiological analyses, we concluded that ecologically relevant and meaningful information can be derived from direct microarray analysis of rRNA in uncharacterized environmental samples, even with the current analytical uncertainty surrounding the behavior of individual probes on tunable bead arrays.


1978 ◽  
Vol 58 (4) ◽  
pp. 475-486 ◽  
Author(s):  
J. C. MUNCH ◽  
Th. HILLEBRAND ◽  
J. C. G. OTTOW

In model experiments under controlled conditions of pH, particle size and amount of glucose, changes in the free Fe oxides (Fed) and acid oxalate soluble Fe (Feo) of six soils were studied after inoculation with iron-reducing, nitrogen-fixing Clostridia. In all soils, the total amount of Fed as well as crystalline Fe (Fed-Feo) decreased immediately and significantly, while Fe(II) in solution increased sigmoidally and was inversely proportional to changes in pH. Feo remained relatively constant throughout the incubation period. As a consequence, the Feo/Fed ratio increased in all samples. At the end of the reduction process, total dissolved Fe(II) correlated with the original Feo content and showed a negative correlation to Fed-Feo. These results are explained by a mechanism in which active non-crystalline iron oxides are reduced in preference to the crystalline forms. The latter are probably transformed and reduced to non-crystalline forms but not until all available active non-crystalline oxides have been exhausted. In the discussion, the reliability of acid oxalate-soluble Fe as a measure of the non-crystalline, active pedogenic iron forms is outlined. Further, the significance of a lowered redox potential (Eh or rH) in reductive transformations of flooded soils is analyzed.


2013 ◽  
Vol 316-317 ◽  
pp. 657-660
Author(s):  
Bei Meng Qi ◽  
Bei Jia Wang ◽  
Chen Guang Wu ◽  
Yi Xing Yuan

Sulfate reducing bacteria (SRB) and iron reducing bacteria (IRB) that widely exist in water supply networks are the main microorganisms leading to metal corrosion in pipelines. Chlorine is widely used in drinking water supply systems. The concentration of chlorine with SRB declined rapidly after 10 mins and reached 0 mg/L finally whereas it decreased more slowly with IRB. If the concentration of chlorine is lower than 0.2mg/L, IRB cannot be sterilized. It indicates that at the end of water pipes where the concentration of chlorine is required to be 0.05mg/L, chlorine is not effective since the concentration is below the minimum requirement of removing IRB


2006 ◽  
Vol 72 (12) ◽  
pp. 7919-7921 ◽  
Author(s):  
E. J. Kerin ◽  
C. C. Gilmour ◽  
E. Roden ◽  
M. T. Suzuki ◽  
J. D. Coates ◽  
...  

ABSTRACT The Hg-methylating ability of dissimilatory iron-reducing bacteria in the genera Geobacter, Desulfuromonas, and Shewanella was examined. All of the Geobacter and Desulfuromonas strains tested methylated mercury while reducing Fe(III), nitrate, or fumarate. In contrast, none of the Shewanella strains produced methylmercury at higher levels than abiotic controls under similar culture conditions. Geobacter and Desulfuromonas are closely related to known Hg-methylating sulfate-reducing bacteria within the Deltaproteobacteria.


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