scholarly journals APPLICATION OF NATURAL PETROLEUM ACIDS AS HEXAMETYLENDIAMIN COMPLEX COMBINES AS BACTERIA

2021 ◽  
Vol 10 (06) ◽  
pp. 58-63
Author(s):  
Teyyub İsmayılov, Sevinj Suleymanova Teyyub İsmayılov, Sevinj Suleymanova
Keyword(s):  
Room Temperature ◽  
Bactericidal Effect ◽  
Complex Compounds ◽  
Chemical Parameters ◽  
Sulfate Reducing ◽  
Bactericidal Properties ◽  
Physical And Chemical ◽  
Reducing Bacteria ◽  
Growth Of Bacteria ◽  
Petroleum Acid

Complex compounds were synthesized by mixing natural petroleum acids with hexamethylene diamine at a ratio of 1:1 and 2:1 at room temperature, IR spectra of the complexes were studied and confirmed, physical and chemical parameters were determined, solutions were prepared and bactericidal properties were studied. The bactericidal effect of a complex compound of natural petroleum acids synthesized with hexamethylenediamine in a ratio of 1: 1 mol at a concentration of 250 mg / l is 95%, a complex combination obtained at a concentration of 500 mg / l and 1000 mg / l is 100%, bactericidal at a concentration of 2: 1 mol at a concentration of 250 mg / l The effect was 98%, 100% at 500 and 1000 mg / l concentrations. According to the results, complex compounds of natural petroleum acids synthesized with hexamethylenediamine can be used to prevent the growth of bacteria. Keywords: natural petroleum acid, hexamethylenediamine, sulfate-reducing bacteria, complex compounds, bactericidal properties.

scholarly journals PREPARATION OF COMPOSITIONS OF OXYETHYLATED NONYLPHENOL WITH ALKYLIMIDAZOLES AND STUDY OF THEIR ACTION AGAINST SULFATE-REDUCING BACTERIA

2021 ◽  
Vol 09 (05) ◽  
pp. 60-65
Author(s):  
Zaur Aghamaliyev,Vagif Abbasov Zaur Aghamaliyev,Vagif Abbasov ◽  
Chingiz Rasulov,Durna Aghamaliyeva Chingiz Rasulov,Durna Aghamaliyeva
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Bactericidal Effect ◽  
Sterile Water ◽  
Sulfate Reducing ◽  
Polyethylene Polyamine ◽  
Three Samples ◽  
Mass Ratios ◽  
Reducing Bacteria ◽  
Number Of Bacteria ◽  
Petroleum Acid

The article discusses the bactericidal effect of compositions of alkylimidazole and oxyethylated para-nonylphenol in various proportions obtained by the interaction of polyethylenepolyamine in a nitrogen medium with various concentrations against sulfate-reducing bacteria. Alkylimidazole was obtained on a pilot plant with a capacity of 500 kg/day, built at the research and production enterprise "Tural 110" in Sumgait. Alkylimidazole (AIM) is derived from the interaction of natural petroleum acid (NPA) with polyethylene polyamine (PEPA). The effect of ONP and AIM compositions prepared in mass ratios of 95:5; 90:10 and 50:50 on the incubation period of SRB for 15 days was studied as follows. Reagent-free and reagent media were used for comparison. 1 ml of the bacterial medium was diluted with sterile water and simultaneously transplanted into a Posgate B nutrient medium in 10 ml test tubes in a 1:9 ratio and kept in a thermostat at 30-35°C for 15 days to determine the number of bacteria growing in the medium. As a result, the number of bacteria in the non-inhibitory medium taken under microscopic control was n=108 the number of cells/ml and in the inhibitory medium at a concentration of 50 mg/l in all three samples (AIM:ONP = 5:95; 10:90 and 50:50 in mass ratios) the number was defined as n=101 cells/ml and at a concentration of 250 mg/l AIM:ONP = 10:90 and AIM:ONP = 50:50, the mass ratios fell from 108 to 0, which completely stopped the life of bacteria. Keywords: natural petroleum acid, polyethylene polyamine, alkylimidazole, oxyethylated nonylphenol, composition, sulfate-reducing bacteria, bactericidal inhibitor.

scholarly journals IDENTIFIKASI BAKTERI RESISTEN MERKURI PADA INDIVIDU DI DAERAH PESISIR PANTAI DI DESA BUDO KECAMATAN WORI

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Jefry Hamonangan Hinonaung
Keyword(s):  
Room Temperature ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Sulfate Reducing ◽  
Liquid Element ◽  
Bottom Waters ◽  
Biochemical Testing ◽  
Biology Laboratory ◽  
Reducing Bacteria ◽  
Mercury Resistant Bacteria

Abstract: Mercury is a silver liquid element at room temperature. Mercury form a variety of both inorganic and organic compounds. Mercury that goes into the sea, there is evaporated back into the atmosphere and fall to the ground experiencing methylation. Mercury in water can undergo methylation area with the help of the sulfate reducing bacteria and iron. Not only mercury from only rainwater but sediment mercury in the bottom waters can also be converted into methyl mercury. MeHg is harmful to humans, because it will accumulate MeHg in plankton or microorganisms. Then the plankton and microorganisms will be eaten by predators higher up the food chain in consumption by humans. This tudy aims to determine determine mercury resistant bacteria found in individuals in the coastal areas. The study design was a descriptive exploratory method. Samples taken in this study was a colony of mercury -resistant bacteria in tartar, urine and feces. Specimens were obtained put in a sterile pot and immediately brought to the biology laboratory in the MIPA Unsrat Manado Faculty to the identification of mercury -resistant bacteria and test. Isolation of mercury resistant bacterial isolates in 3 samples, obtained 6 isolates. Then test to identify bacteria with morphological, physiological testing, and biochemical testing. Results of a study found four genus of bacteria. Keywords: Mercury , Mercury Resistant Bacteria , Tartar , Urine , Feces     Abstrak:Merkuri merupakan suatu unsur berbentuk cair keperakan pada suhukamar. Merkuri membentuk berbagai persenyawaan baik anorganikmaupun organik. Merkuri yang masuk ke dalam laut, ada yang menguap kembali ke atmosfir dan jatuh ke tanah mengalami metilisasi. Merkuri dalam daerah perairan dapat mengalami metilisasi dengan bantuan bakteri pereduksi sulfat dan besi. Tidak hanya merkuri dari air hujan saja tetapi sedimen merkuri di dasar perairan juga dapat diubah menjadi metil merkuri. MeHg ini berbahaya bagi manusia, karena MeHg ini akan terakumulasi dalam plankton atau mikroorganisme. Kemudian plankton dan mikroorganisme ini akan di makan oleh predator yang lebih tinggi lagi dalam rantai makanan hingga di konsumsi oleh manusia. Penelitian ini bertujuan untuk mengetahui mengetahui bakteri resisten merkuri yang terdapat pada individu di daerah pesisir pantai. Desain penelitian adalah metode deskriptif eksploratif. Sampel yang diambil dalam penelitian ini adalah koloni bakteri resisten merkuri pada karang gigi, urin dan feses. Spesimen yang didapatkan dimasukkan ke dalam pot steril dan segera di bawa ke laboratoriumbiologi Fakultas MIPA Unsrat Manado untuk dilakukan identifikasi bakteri dan uji resisten merkuri. Isolasi isolat bakteri resisten merkuri pada 3 sampel, diperoleh 6 isolat. Kemudian dilakukan identifikasi bakteri dengan uji morfologi, uji fisiologi, dan uji biokimia. Hasil peneltian ditemukan 4 genus bakteri. Kata kunci: Merkuri, Bakteri Resisten Merkuri, Karang gigi, Urin, Feses

scholarly journals Destruction of toluene and xylene by sulfatе-reducing bacteria

2019 ◽  
Vol 30 (2) ◽  
pp. 95-100 ◽  
Author(s):  
N. S. Verkholiak ◽  
T. B. Peretyatko
Keyword(s):  
Hydrogen Sulfide ◽  
Aromatic Hydrocarbons ◽  
Aromatic Compounds ◽  
Sulfate Reducing Bacteria ◽  
Control Medium ◽  
Sulfate Ion ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Growth Of Bacteria

As a result of human activity aromatic hydrocarbons enter the environment in large quantities, contaminating it. Dropping of insufficiently treated wastewater drains considerably decrease the quality of water. Quite effective biological methods of purification of contaminated environment are the usage of microorganisms. Prospective microorganisms for sewage treatment are sulfate-reducing bacteria. The purpose of the work was to investigate the ability of sulfate-reducing bacteria to use xylene and toluene as a source of carbon under different cultivation conditions.  The study objects were sulfate-reducing bacteria Desulfotomaculum AR1 and Desulfovibrio desulfuricans Ya-11. The biomass of bacteria was determined turbidimetrically, the content of sulfate ion and hydrogen sulfide – photometrically in the culture fluid. The content of fumarate was determined by the method of high-performance liquid chromatography. The ability of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria to use toluene and xylene as the sole source of carbon and energy has been established. In the toluene environment, a better growth of bacteria was observed. A comparison was made between the efficiency of the reduction of sulfate ions and the growth of bacteria in the control medium and the medium with aromatic compounds. The efficiency of sulfate ions reduction was sufficiently high in the control medium, whereas in the toluene/xylene media the efficiency of sulfate utilization and hydrogen sulfide accumulation was lower compared to the control parameters. The growth of Desulfotomaculum AR1 and D. desulfuricans Ya-11 sulfate-reducing bacteria was investigated in xylene and toluene media in the presence/absence of fumarate. According to the results of the studies, the best growth of the tested bacteria was observed in the medium with aromatic compounds in the presence of fumarate and sulfate ion. Efficiency of use of fumarate in the medium with toluene with bacteria Desulfotomaculum AR1 was more than 90 %. Fumarate can be used by sulfate-reducing bacteria as a source of carbon, donor and acceptor of electrons. Fumarate is most likely to inhibit sulfate reduction in Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria, as indicated by studies showing that sulfate reduction efficiency in lactate, fumarate, and sulfate ion media was half that of control. Sulfate-reducing bacteria are capable to anaerobically degrade aromatic hydrocarbons in the presence of sulfate ions as terminal electron acceptors. The ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to use the aromatics of the BTEХ – toluene, xylene and sulfate-ions, makes them promising at the biological purification step of contaminated wastewater from these pollutants.

scholarly journals The antimicrobial activity of alcoholic extracts of leaves and bark from two varieties of ”Sangre de Drago” compared with the antimicrobial activity present in the latex of the same species

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1200
Author(s):  
Cecilia Barba Guevara ◽  
Luis Montaluisa ◽  
María Elena Maldonado Rodriguez
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Room Temperature ◽  
Medicinal Products ◽  
Bacterial Strains ◽  
Chemical Parameters ◽  
E Coli ◽  
Quality Control Standard ◽  
Physical And Chemical ◽  
Antimicrobial Effectiveness

Background: This research was carried out in order to study the antimicrobial effectiveness of crude latex of two varieties of "Sangre de Drago": Croton lechleri Muller Arg. and Croton urucurana Baill and compare that effectiveness to the antimicrobial activity of the alcoholic extracts of its leaves and bark.  Methods: The activity of the alcoholic extracts and latex were evaluated against bacterial strains of Staphylococcus epidermidis, Bacillus subtilis and Escherichia coli. The extraction of the alcoholic extracts (20% Tincture) of the leaves, bark and latex from the two Croton species was carried out by maceration using 70% alcohol as a menstruum, at room temperature, for 2 to 7 days, with shaking at least twice a day.  A 20% tincture was obtained, from which the physical and chemical parameters were determined as indicated by the Ecuadorian Quality Control Standard for natural medicinal products. Results: It was found that both the alcoholic extracts of the plant material and the crude latex indicate antimicrobial activity for S. epidermidis, moderate antimicrobial activity for B. subtilis and no antimicrobial activity for E. coli. The moderate antimicrobial activity against B. subtilis, at doses of 125 p.p.m., is in line with the findings of previous studies by other authors.  Conclusions: he antimicrobial activity of the latex of the two species against S. epidermidis is not registered in literature and, the negative antimicrobial activity for E. coli does not agree with what has been reported by previous studies.

Treatment of Acid Mine Drainage by Sulfate Reducing Bacteria and Iron at Room Temperature

2010 ◽  
Vol 113-116 ◽  
pp. 1500-1503
Author(s):  
Ying Feng ◽  
Yong Kang ◽  
Yan Fang Yu
Keyword(s):  
Room Temperature ◽  
Mine Drainage ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Treatment Efficiency ◽  
Sulfate Reducing ◽  
Acid Mine ◽  
Mine Wastewater ◽  
Reducing Bacteria ◽  
Acid Mining Drainage

This study describes a new method to treat acid mine wastewater containing high amounts of heavy metals and sulfate by biotechnology. Sulfate reducing Bacteria (SRB) was inoculated in an up-flow multiple bed bioreactor treating practical wastewater. In addition to precipitation processes, water purification was also possible with the metabolism process of microorganisms. Iron dust was added to the system to enhance the activity of SRB and ensure the treatment efficiency. The results indicates that treating acid mining drainage using SRB and iron at room temperature (20°C~25°C) is possible, the reduction rate of sulfate is up to 61%, pH of wastewater raises from 2.75 to 6.2 and the copper concentration of effluent is less than 0.2 mg/L.

Bactericidal properties of a combined corrosion inhibitor in the presence of SRB

2020 ◽  
Vol 25 (3) ◽  
Keyword(s):  
Hydrogen Sulfide ◽  
Hydrogen Diffusion ◽  
Desulfovibrio Desulfuricans ◽  
Bacterial Cells ◽  
Bacteriostatic Effect ◽  
Sulfate Reducing ◽  
Bactericidal Properties ◽  
Number Of Microorganisms ◽  
Reducing Bacteria ◽  
Steel St3

The bactericidal properties of the combined inhibitor with respect to two types of Desulfovibrio desulfuricans and Desulfomicrobium sulfate-reducing bacteria were studied. The effect of the inhibitor on the number of bacterial cells and the formation of hydrogen sulfide in Postgate nutrient Postgate “B” was evaluated. It has been shown that the combined inhibitor exhibits a bacteriostatic effect on to sulphate-reducing bacteria. It was revealed that the degree of suppression of the number of microorganisms Desulfovibrio desulfuricans at a concentration of the combined inhibitor 100,0 mg/L is higher than Desulfomicrobium. In the latter case, to achieve this effect, 120,0 mg/L concentration of the combined inhibitor is required. The studied combination inhibitor causes the inhibition of hydrogen diffusion in steel St3 in the MI medium saturated with H2S and CO2 separately and together, and also contributes to preserving the ductile properties of the steel St3 after exposure to solutions compared to non-inhibited media.

Failure Analysis of Tee in Shale Gas Transportation Platform

2021 ◽  
Vol 1035 ◽  
pp. 439-446
Author(s):  
Nan Ji ◽  
Jie Feng ◽  
Yan Long
Keyword(s):  
Shale Gas ◽  
Service Condition ◽  
Sulfate Reducing Bacteria ◽  
Metallographic Examination ◽  
Internal Corrosion ◽  
Corrosion Failure ◽  
Sulfate Reducing ◽  
Electron Microscope Analysis ◽  
Physical And Chemical ◽  
Reducing Bacteria

The mains reasons for internal corrosion failure and cracking of the tee in shale gas transportation platform have been proposed based on macroscopic analysis, physical and chemical property test, metallographic examination, Scanning Electron Microscope analysis, X-ray spectroscopy analysis and service condition analysis. The results show that corrosion occurring in the inner wall of tee was caused by the combined impacts of CO2 corrosion, sulfate-reducing bacteria (SRB) corrosion and erosion corrosion. The crack in the weld of the inner wall was mainly due to the Sulfate-reducing Bacteria Induced cracking.

scholarly journals Investigation of the inhibitory-bactericidal effect of amidoamine-based inorganic complexes against microbiological and atmospheric corrosion

2020 ◽  
Vol 22 (3) ◽  
pp. 29-37
Author(s):  
Y.J. Aghazada ◽  
V.M. Abbasov ◽  
N.I. Mursalov ◽  
S.E. Abdullayev ◽  
U.J. Yolchuyeva
Keyword(s):  
Metal Surface ◽  
Atmospheric Corrosion ◽  
Isopropyl Alcohol ◽  
Bactericidal Effect ◽  
Corrosion Process ◽  
Inorganic Complexes ◽  
Correlation Constant ◽  
Reducing Bacteria ◽  
Kinetics Of ◽  
Petroleum Acid

AbstractIn the process, inorganic complexes of amidoamines obtained from the interaction of natural petroleum acid and oleic acids with diethylenetriamine have been developed and their effectiveness as inhibitor-bactericides has been investigated. The effect of the synthesized reagents on the kinetics of the corrosion process of steel and the activity of sulphate-reducing bacteria in 3% NaCl solution saturated with CO2 and in the biphasic water–isopropyl alcohol medium with H2S dissolved has been analyzed. The thermodynamic and kinetic parameters of the corrosion process were calculated. The adsorption of the complexes was investigated using the Langmuir isotherm and the correlation constant was determined. State of the metal surface was investigated by SEM method in CO2 and H2S media, with and without inhibitors, and the metal surface contact of complexes was studied by computer molecular simulation.

Evaluation of Minimally Invasive Arthroscopy Technology in Sports Medicine—An Evaluation of Nano Silver Disinfectants

2021 ◽  
Vol 21 (3) ◽  
pp. 1446-1450
Author(s):  
Ruiyi Ge ◽  
Jin Zhang ◽  
Ziquan Yang
Keyword(s):  
Particle Size ◽  
Sports Medicine ◽  
Anaerobic Bacteria ◽  
Surgical Instruments ◽  
Nano Silver ◽  
Iron Reducing Bacteria ◽  
Sulfate Reducing ◽  
Bactericidal Properties ◽  
Reducing Bacteria ◽  
Uniform Particle Size

Currently, nano silver fungicide prepared in the laboratory is used to disinfect arthroscopic surgical instruments. In this study, nano silver fungicides with stable properties were prepared and characterized. Afterwards, their bactericidal properties as well as mucus peeling properties were further tested. The results show that the nano silver fungicide prepared here contains uniform particle size and displays material stability for 60 days. Nano silver fungicide can completely kill sulfate-reducing bacteria, anaerobic bacteria, and iron reducing bacteria, while the slime stripping rate is 80.58%. Additionally, we propose the use of nano silver sterilization agents to kill the arthroscopic surgical instruments in conjunction with proper manual cleaning, as they can effectively kill all the bacteria on the surgical instruments, achieving a sterilization rate of 99.99%.

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