Green-Route Mediated Synthesis of Silver Nano-Particles from Aegle marmelos and Evaluating Its Antimicrobial Activity

2017 ◽  
Vol 6 (3) ◽  
pp. 297-304 ◽  
Author(s):  
Sabita Singh ◽  
Kunal Biswas ◽  
Sanhita Chowdhury ◽  
Yugal Kishore Mohanta ◽  
Jaya Bandyopadhyay
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.


RSC Advances ◽  
2014 ◽  
Vol 4 (29) ◽  
pp. 15022-15029 ◽  
Author(s):  
Totan Ghosh ◽  
Sandeep Kumar Dash ◽  
Prateeti Chakraborty ◽  
Averi Guha ◽  
Kenji Kawaguchi ◽  
...  

Two varieties of Co3O4 nano particles (Co3O4-I and Co3O4-II) have been synthesized from two different precursors using a pyrolytic technique and their magnetic and in vitro antimicrobial activity have been investigated.


2018 ◽  
Vol 12 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Oluwafemi A. Ojo ◽  
Babatunji E. Oyinloye ◽  
Adebola B. Ojo ◽  
Basiru O. Ajiboye ◽  
Israel I. Olayide ◽  
...  

2017 ◽  
Vol 13 (3) ◽  
pp. 6065-6075
Author(s):  
Manikandan Kandasamy

  In this research work, the effect of Tungsten-doping on the crystal structure, morphology and antimicrobial of titanium dioxide nanoparticles were studied. The pure and different weight % of tungsten doped TiO2 nanoparticles were synthesized by sol–gel method and calcinated at 600°C for 5 hours. The synthesised products have been characterized by X-ray Diffraction studies (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), Elemental analysis (EDXA), Ultra Violet Visible Spectroscopy (UV-Vis), Photoluminescence Spectra (PL), High Resolution Transmission Spectroscopy (HRTEM) and Fourier Transform Infra Red Spectroscopy (FT-IR). XRD pattern of pure TiO2 and 1 weight % W-doped TiO2nanoparticles confirms the anatase structure and increase in the W-doping changes the phase of TiO2 to rutile. Average crystallite size of synthesized nanoparticles was determined using the Debye–Scherrer formula. The crystallite size obtained for pure TiO2 is in the range from 29 nm to 39 nm and W-doped TiO2 is varied from 28 to 34 nm. The SEM images show the agglomerated particles of spherical-like morphology. Optical property and direct bandgap of pure and W-doped TiO2nanoparticles also further characterised by UV–Vis Spectroscopy. The images of HRTEM clearly confirm that particles present in the W-doped TiO2 powdered sample is nanosized particles. The Kirby Bauer Agar Well Diffusion Assay  method was employed to explore antimicrobial activity of nanosized pure and W-doped TiO2 colloidal suspension against the test microorganisms two Gram positive bacteria(Staphylococcus aureus, Bacillus subtilis),two Gram negative Bacteria(Escherichia coli, Pseudomonas aeruginosa),and two fungi(Candida albicans, Aspergillus niger). It shows that the W- doped TiO2 nanoparticles inhibited the multiplication and growth of the above mentioned test bacteria and fungi. Antimicrobial activity was found against all tested microorganisms which confirmed that W-doped TiO2 nanoparticles possess high antimicrobial activity compared to pure TiO2 nanoparticles.   


Author(s):  
Jaishree Sharma ◽  
Padma Kumar

Objective: The present study was carried out to evaluate the in vitro antimicrobial activity of water, methanolic and pet ether extracts from leaves and fruit of A. marmelos and C. papaya. Methods: Crude extract of leaves and fruit of Aegle marmelos and Carica papaya were prepared for series of polar solvents by hot extraction method in soxhlet then the extracts were first screened for its antimicrobial activity by “Disc Diffusion Assay” against medically important bacteria, plant pathogen and fungi. Fractions showing activities were then used to determine MIC (minimum inhibitory concentration) and MBC/MFC (minimum bactericidal and fungicidal concentration) by broth dilution and total activity were also calculated.Result: Aegle marmelos shows comparatively significant antimicrobial activity than Carica papaya. However antimicrobial activity was found both solvent and organism dependent. Almost all the extracts of Aegle marmelos show an inhibitory effect against most of the test organism whereas the test extracts Carica papaya show an inhibitory effect against the very few test organisms. Lowest MIC values 0.0195 mg/ml were recorded against Roultella planticola and Klebsiella pneumonae indicate the significant antimicrobial potential of the test extracts. The high value of TA was recorded against Roultella planticola in both the plants. Data were analyzed by one way ANNOVA and values were considered significant at P<0.05Conclusion: The MIC and MBC/MFC values of Aegle marmelos showed its broad antimicrobial potential and is promising in the development of phytomedicine for antimicrobial properties then Carica papaya.


Author(s):  
Riham Omar Bakr ◽  
Soumaya Saad Zaghloul ◽  
Reham Ibrahim Amer ◽  
Dalia Abd Elaty Mostafa ◽  
Mahitab Helmy El Bishbishy

Objective: Aegle marmelos (L.) Correa has been widely used in Indian traditional medicine and has many reported pharmacological activities. The aim of this research was to formulate solid lipid nanoparticles (SLNs) of Aegle oil (AO) that enhanced the beneficial antimicrobial activity of the oil. Methods: The chemical composition of Aegle leaf essential oil was analysed by GC-MS. Additionally, a phytochemical study of A. marmelos methanolic leaf extract was conducted using Folin-Ciocalteu colorimetric assay for determination of total phenolic content as well as ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-ESI-MS-MS) analyses for identification of individual components. Six formulations of AO-loaded SLNs (AO-SLNs) were prepared by a double emulsification method. The particle size, zeta potential (ZP), polydispersibility index (PDI) and drug encapsulation efficiency (EE) of the SLNs were determined. The morphology of the SLNs was observed by transmission electron microscopy (TEM). The antimicrobial activity of AO and AO-SLNs was assessed using disc diffusion method. Results: Thirty-two compounds were identified in the Aegle oil, of which Δ-carene and α-phellandrene were the most abundant (48.14% and 34.14%, respectively). The estimated total phenolic content was 968mg gallic acid equivalents (GAE)/g, while UPLC-ESI-MS-MS led to the tentative characterization of thirteen metabolites. The SLNs showed ZP, PDI and EE 125 ± 0.22nm, –37.85, 0.282, and 92%, respectively. AO and AO-SLNs showed significant antimicrobial activity, and the SLNs could sustain the release of AO from their gel vehicles. Conclusion: Our results provide evidence for the application of AO-SLNs in topical and transdermal delivery systems.


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