The study on knee osteoarthritis in diabetic patients based on nanometric magnetic bead method

2020 ◽  
Vol 10 (8) ◽  
pp. 1271-1277
Author(s):  
Dongbin Luo ◽  
Dabiao Hou ◽  
Simin Luo
Keyword(s):  
Inflammatory Cytokines ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Interleukin 1 ◽  
Magnetic Bead ◽  
Inflammatory Factors ◽  
Control Group ◽  
Diabetic Patients ◽  
Peripheral Blood Mononuclear ◽  
Bead Method

To study the levels of inflammatory cytokines in synovia fluid from osteoarthritis (OA) patients with/without type 2 diabetes mellitus (T2D). Out of 74 volunteers aged 20–88 years, 64 with knee OA (Kellgren-Lawrence grades over I) were recruited and divided into groups with (n = 20) and without (n = 44) diabetes. The nucleic acid of the patient tissue was extracted by nanometer magnetic bead method. The remaining participants were the control group (n = 10). Synovia fluid, sera, and peripheral blood mononuclear cell (PBMC) samples were collected from all participants and analyzed using ELISA Kits. T2D-OA patients and OA patients had higher basal production of interleukin-1, (IL-1), interleukin-6 (IL-6), and lower-level interleukin-10 (IL-10). However, there were no differences between T2D-OA and OA patients. IL-1, IL-10, and IL-6 in peripheral blood mononuclear cells (PBMCs) after stimulation with LPS were obviously up-regulated in both patients and controls. The production of IL-1 and IL-6 in synovia from T2D-OA and OA patients increased as in the case of variations in sera and PBMCs. Both T2D-OA and OA patients had high-levels of IL-1 and IL-6 compared with controls, especially IL-6. The presence of IL-10 could not be detected in synovia from both patients and controls. Our results suggested that OA patient also had some pro-inflammatory factors in vivo, especially in local lesion; this manifest was particularly evident in T2D-OA patients. The high-level concentration of pro-inflammatory cytokines and low-level anti-inflammatory factors could be one of the reasons why T2D-OA patients are prone to developing synovitis.

scholarly journals Autologous Peripheral Blood Mononuclear Cells for Limb Salvage in Diabetic Foot Patients with No-Option Critical Limb Ischemia

2021 ◽  
Vol 10 (10) ◽  
pp. 2213
Author(s):  
Alessia Scatena ◽  
Pasquale Petruzzi ◽  
Filippo Maioli ◽  
Francesca Lucaroni ◽  
Cristina Ambrosone ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Critical Limb Ischemia ◽  
Diabetic Foot ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Limb Ischemia ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Peripheral blood mononuclear cells (PBMNCs) are reported to prevent major amputation and healing in no-option critical limb ischemia (NO-CLI). The aim of this study is to evaluate PBMNC treatment in comparison to standard treatment in NO-CLI patients with diabetic foot ulcers (DFUs). The study included 76 NO-CLI patients admitted to our centers because of CLI with DFUs. All patients were treated with the same standard care (control group), but 38 patients were also treated with autologous PBMNC implants. Major amputations, overall mortality, and number of healed patients were evaluated as the primary endpoint. Only 4 out 38 amputations (10.5%) were observed in the PBMNC group, while 15 out of 38 amputations (39.5%) were recorded in the control group (p = 0.0037). The Kaplan–Meier curves and the log-rank test results showed a significantly lower amputation rate in the PBMNCs group vs. the control group (p = 0.000). At two years follow-up, nearly 80% of the PBMNCs group was still alive vs. only 20% of the control group (p = 0.000). In the PBMNC group, 33 patients healed (86.6%) while only one patient healed in the control group (p = 0.000). PBMNCs showed a positive clinical outcome at two years follow-up in patients with DFUs and NO-CLI, significantly reducing the amputation rate and improving survival and wound healing. According to our study results, intramuscular and peri-lesional injection of autologous PBMNCs could prevent amputations in NO-CLI diabetic patients.

Interleukin-1α Polymorphism -889C/T Related to Obesity in Korean Taeumin Women

2008 ◽  
Vol 36 (01) ◽  
pp. 71-80 ◽  
Author(s):  
Jeong-Sub Song ◽  
Hyun-Ja Jeong ◽  
Su-Jin Kim ◽  
Mu-Song Son ◽  
Ho-Jeong Na ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Interleukin 1 ◽  
Regulatory Region ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Metabolic Functions ◽  
Sasang Constitution ◽  
Korean Traditional Medicine ◽  
Interleukin 1Α ◽  
Major Branch

Interleukin-1 (IL-1), a cytokine produced predominantly by cells from the macrophage lineage, can affect multiple neuroendocrine and metabolic functions. IL-1α production by cultured peripheral blood mononuclear cells from an obese group was significantly elevated in comparison to a control group. The aim of this study was to investigate whether the IL-1α polymorphism and Sasang constitution, a major branch in Korean traditional medicine, were related to obesity. Genotyping was done in 182 healthy females with a marked variation in body mass index (BMI) by a PCR-restriction fragment length polymorphism assay. The T allele was associated with decreased BMI (p = 0.020). In a subgroup with BMI values ranging from 27 ~ 29 kg/m2, the frequency of the T allele was significantly decreased (p = 0.004, odds ratio, OR = 0.141 compared to a subgroup with a BMI values less than 25 Kg/m2). In addition, in Taeumin female subjects, the frequency of the IL-1α T allele was markedly decreased in a subgroup with BMI values in the range of 27 ~ 29 kg/m2compared to a lean group with BMI values less than 25 kg/m2(p = 0.004, OR = 0.139). In Korean women, an association was found between -889C/T polymorphism in the regulatory region of the IL-1α gene and BMI values. In addition, an association was found among IL-1α polymorphism, obesity, and the Sasang constitution.

scholarly journals Interaction of Candida albicans with Adherent Human Peripheral Blood Mononuclear Cells Increases C. albicans Biofilm Formation and Results in Differential Expression of Pro- and Anti-Inflammatory Cytokines

2007 ◽  
Vol 75 (5) ◽  
pp. 2612-2620 ◽  
Author(s):  
Jyotsna Chandra ◽  
Thomas S. McCormick ◽  
Yoshifumi Imamura ◽  
Pranab K. Mukherjee ◽  
Mahmoud A. Ghannoum
Keyword(s):  
Candida Albicans ◽  
Inflammatory Cytokines ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Biofilm Formation ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Laser Microscopy ◽  
Blood Mononuclear Cells ◽  
Monocytes And Macrophages

ABSTRACT Monocytes and macrophages are the cell types most commonly associated with the innate immune response against Candida albicans infection. Interactions between the host immune system and Candida organisms have been investigated for planktonic Candida cells, but no studies have addressed these interactions in a biofilm environment. In this study, for the first time, we evaluated the ability of C. albicans to form biofilms in the presence or absence of adherent peripheral blood mononuclear cells (PBMCs; enriched for monocytes and macrophages by adherence). Our analyses using scanning electron and confocal scanning laser microscopy showed that the presence of PBMCs enhanced the ability of C. albicans to form biofilms and that the majority of PBMCs were localized to the basal and middle layers of the biofilm. In contrast to the interactions of PBMCs with planktonic C. albicans, where PBMCs phagocytose fungal cells, PBMCs did not appear to phagocytose fungal cells in biofilms. Furthermore, time-lapse laser microscopy revealed dynamic interactions between C. albicans and PBMCs in a biofilm. Additionally, we found that (i) only viable PBMCs influence Candida biofilm formation, (ii) cell surface components of PBMCs did not contribute to the enhancement of C. albicans biofilm, (iii) the biofilm-enhancing effect of PBMCs is mediated by a soluble factor released into the coculture medium of PBMCs with C. albicans, and (iv) supernatant collected from this coculture contained differential levels of pro- and anti-inflammatory cytokines. Our studies provide new insight into the interaction between Candida biofilm and host immune cells and demonstrate that immunocytes may influence the ability of C. albicans to form biofilms.

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