Revealing Lipid Body Formation and its Subcellular Reorganization in Oleaginous Microalgae Using Correlative Optical Microscopy and Infrared Nanospectroscopy Atomic Force Microscopy-Induced Resonance (AFM-IR)

2021 ◽  
pp. 000370282110506
Author(s):  
Ariane Deniset-Besseau ◽  
Rémy Coat ◽  
Benjamin Moutel ◽  
Rolando Rebois ◽  
Jérémie Mathurin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Microscopy ◽  
Total Lipid Content ◽  
Lipid Body ◽  
Cellular Level ◽  
Local Analysis ◽  
Label Free ◽  
Force Microscopy ◽  
Oleaginous Microalgae ◽  
Atomic Force

The purpose of this work is to develop an integrated imaging approach to characterize without labeling at the sub-cellular level the formation of lipid body droplets (LBs) in microalgae undergoing nitrogen starvation. First conventional optical microscopy approaches, gas chromatography, and turbidimetry measurements allowed to monitor the biomass and the total lipid content in the oleaginous microalgae Parachlorella kesslerii during the starvation process. Then a local analysis of the LBs was proposed using an innovative infrared nanospectroscopy technique called atomic force microscopy-based infrared spectroscopy (AFM-IR). This label-free technique assessed the formation of LBs and allowed to look into the LB composition thanks to the acquisition of local infrared spectra. Last correlative measurements using fluorescence microscopy and AFM-IR were performed to investigate the subcellular reorganization of LB and the chloroplasts.

scholarly journals Local analysis of semiconductor nanoobjects by scanning tunneling atomic force microscopy

2015 ◽  
Vol 1 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Natalia A. Lashkova ◽  
Nikita V. Permiakov ◽  
Alexander I. Maximov ◽  
Yulia M. Spivak ◽  
Vyacheslav A. Moshnikov
Keyword(s):  
Atomic Force Microscopy ◽  
Local Analysis ◽  
Scanning Tunneling ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy of Annealed Plain Carbon Steels

2015 ◽  
Author(s):  
Surendra Kumar Gupta ◽  
Patricia Iglesias Victoria
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Microscopy ◽  
Interlamellar Spacing ◽  
Lamellar Spacing ◽  
Optical Microscope ◽  
Carbon Steels ◽  
Force Microscopy ◽  
Atomic Force

Microstructure of annealed plain carbon steels is examined using optical microscopy. When the inter-lamellar spacing in pearlite is small, optical microscope at 1000X is unable to resolve the ferrite and cementite lamellae. In hyper-eutectoid steels, cementite in pearlite appears as darker phase whereas the pro-eutectoid cementite appears as a lighter phase. Atomic force microscopy (AFM) of etched steels is able to resolve ferrite and cementite lamellae in pearlite at similar magnifications. Both cementite in pearlite as well as pro-eutectoid cementite appear as raised areas (hills) in AFM images. Interlamellar spacing in pearlite increases with increasing hardenability of steel.

Precision Sinusoidal Local Scan for Large-Range Atomic Force Microscopy With Auxiliary Optical Microscopy

2015 ◽  
Vol 20 (1) ◽  
pp. 226-236 ◽  
Author(s):  
Chih-Lieh Chen ◽  
Jim-Wei Wu ◽  
Yi-Ting Lin ◽  
Li-Chen Fu ◽  
Mei-Yung Chen
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Microscopy ◽  
Large Range ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Label-free identification and characterization of living human primary and secondary tumour cells

The Analyst ◽  
2015 ◽  
Vol 140 (15) ◽  
pp. 5162-5168 ◽  
Author(s):  
Dimitrios Tsikritsis ◽  
Susanna Richmond ◽  
Patrick Stewart ◽  
Alistair Elfick ◽  
Andrew Downes
Keyword(s):  
Raman Spectroscopy ◽  
Atomic Force Microscopy ◽  
Tumour Cells ◽  
Label Free ◽  
Force Microscopy ◽  
Atomic Force ◽  
Secondary Tumour ◽  
Identification And Characterization

Primary and secondary tumour cells exhibit biochemical differences (with Raman spectroscopy and imaging), and mechanical differences (with atomic force microscopy).

Label-free quantification of activated NF-κB in biological samples by atomic force microscopy

2010 ◽  
Vol 25 (11) ◽  
pp. 2490-2496 ◽  
Author(s):  
Michele Menotta ◽  
Rita Crinelli ◽  
Elisa Carloni ◽  
Marzia Bianchi ◽  
Elisa Giacomini ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Biological Samples ◽  
Label Free ◽  
Force Microscopy ◽  
Label Free Quantification ◽  
Atomic Force ◽  
Free Quantification

AFM Study of Typical Fracture Surfaces in Room-Temperature Fracture of Sapphire

2009 ◽  
Vol 409 ◽  
pp. 113-122
Author(s):  
José M. López-Cepero ◽  
Sheldon M. Wiederhorn ◽  
António Ramirez de Arellano-López ◽  
Julian Martínez-Fernández
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Microscopy ◽  
Special Interest ◽  
Room Temperature ◽  
Crystalline Orientation ◽  
Force Microscopy ◽  
Fracture Surfaces ◽  
Atomic Force ◽  
Temperature Fracture

Rhombohedral r-plane fracture surfaces in sapphire are analyzed by optical microscopy and by atomic force microscopy. Features of special interest include steps, lines and angles on the surface that appear to have crystallographic origins. A classification and description of these features is given over a scale ranging from hundreds of micrometers to tens of nanometers. Preferential directions in the surface are identified and related to the crystalline orientation of the sample; an attempt is made to identify the underlying phenomenology behind the appearance of each kind of feature.

Sign in / Sign up

Export Citation Format

Share Document