Synthesis of bioactive material by sol–gel process utilizing polymorphic calcium carbonate precipitate and their direct and indirect in-vitro cytotoxicity analysis

2020 ◽  
Vol 18 ◽  
pp. 100647 ◽  
Author(s):  
Manish Kumar ◽  
Edgard Gnansounou ◽  
Indu Shekhar Thakur
Keyword(s):  
Calcium Carbonate ◽  
Sol Gel ◽  
In Vitro Cytotoxicity ◽  
Calcium Carbonate Precipitate ◽  
Bioactive Material ◽  
Sol Gel Process

In Vitro Cytotoxicity of Alginate-Encapsulating Ferrite Particles Using WST-1

2005 ◽  
Vol 284-286 ◽  
pp. 815-818
Author(s):  
Sang Bae Lee ◽  
Se Ho Lee ◽  
D.H. Kim ◽  
Doug Youn Lee ◽  
Yong Keun Lee ◽  
...  
Keyword(s):  
Cell Viability ◽  
Calcium Alginate ◽  
Fetal Bovine Serum ◽  
Sol Gel ◽  
In Vitro Cytotoxicity ◽  
Distilled Water ◽  
Sol Gel Process ◽  
Fetal Bovine ◽  
Alginate Content

The purpose of this study was to evaluate the cytotoxicity of alginate-encapsulting ferrite particles in vitro. Various ferrite particles such as Ba-ferrite, Sr-ferrite, Co-ferrite, Co/Ni-ferrite were prepared by sol-gel process. Ferrite particles were encapsulated via calcium alginate process with different alginate contents ranged from 10 to 100 wt%. Mouse-fibroblastic NCTC L-929 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum. The alginate-encapsulating ferrites were extracted in 5 ml of distilled water under pH 6.5 at 121°C for 1 h in accordance with ISO 10993-12. In vitro cytotoxicity was evaluated by WST-1. The results of this study indicated that the alginate-encapsulting ferrite particles affected cell viability by increasing alginate contents. Especially, alginate-encapsulating process were enhanced cell viability of ferrites such as Sr-ferrite, Co/Ni-ferrite, and Ba-Ferrite when alginate content was 10 wt%.

scholarly journals Synthesis, characterization and in vitro cytotoxicity study of Co and Ni ferrite nanoparticles prepared by sol-gel method

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
Alexandre Pancotti ◽  
Dener Pereira Santos ◽  
Dielly Oliveira Morais ◽  
Mauro Vinícius de Barros Souza ◽  
Débora R. Lima ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Sol Gel ◽  
Test Sample ◽  
In Vitro Cytotoxicity ◽  
Gel Method ◽  
X Ray ◽  
Sol Gel Method ◽  
Transmission Electron ◽  
Crystallite Sizes

AbstractIn this study, we report the synthesis and characterization of NiFe2O4 and CoFe2O4 nanoparticles (NPs) which are widely used in the biomedical area. There is still limited knowledge how the properties of these materials are influenced by different chemical routes. In this work, we investigated the effect of heat treatment over cytotoxicity of cobalt and niquel ferrites NPs synthesized by sol-gel method. Then the samples were studied using transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), Fourier Transform Infrared Spectroscopy Analysis (FTIR), and X-ray fluorescence (XRF). The average crystallite sizes of the particles were found to be in the range of 20–35 nm. The hemocompatibility (erythrocytes and leukocytes) was checked. Cytotoxicity results were similar to those of the control test sample, therefore suggesting hemocompatibility of the tested materials.

Apatite Formation on PDMS-Modified SiO2-CaO-P2O5 Hybrids Prepared with Different P2O5 Content by Sol-Gel Method

2004 ◽  
Vol 449-452 ◽  
pp. 1121-1124 ◽  
Author(s):  
Do Won Seo ◽  
J.G. Kim ◽  
Yun Hae Kim ◽  
Chin Myung Whang
Keyword(s):  
Sol Gel ◽  
Calcium Nitrate ◽  
Apatite Formation ◽  
Calcium Nitrate Tetrahydrate ◽  
X Ray Diffraction ◽  
Phosphate Ions ◽  
P2o5 Content ◽  
Sol Gel Process ◽  
Nitrate Tetrahydrate

Bioactive ORMOSILS (organically modified silicate), PDMS-CaO-SiO2-P2O5 with five different P2O5 content (0, 0.01, 0.03, 0.06, 0.09 mol%) have successfully been synthesized by sol-gel process. The hybrids have been prepared with polydimethylsiloxane (PDMS), tetraethoxysilane (TEOS), calcium nitrate tetrahydrate [Ca(NO3)2 4H2O] and triethyl phosphate (TEP) as starting materials and subsequently soaked into the simulated body fluid (SBF) for different period of time and the bioactivity of hybrids was determined by examining the apatite formation on the surface of the specimen by FT-IR, Thin-Film X-ray Diffraction, and Scanning Electron Microscopy (SEM). All of the prepared samples with different P2O5 content showed in vitro bioactivity. It was observed that the increase in P2O5 content up to 0.03 mole % increases the apatite formation compared to P2O5- free hybrids. However, further increase in P2O5 concentration slows down the formation of the apatite layer most probably due to the decrease of pH of SBF by dissolution of a large amount of phosphate ions.

Novel Organic-Inorganic Hybrid Nanoparticles as Non-Viral Gene Vector

2008 ◽  
Vol 47-50 ◽  
pp. 1319-1322
Author(s):  
Yang Zhao ◽  
Pei Yin ◽  
Zu Yong Wang ◽  
Lei Ren ◽  
Qi Qing Zhang
Keyword(s):  
Gene Therapy ◽  
Encapsulation Efficiency ◽  
Sol Gel ◽  
Hybrid Nanoparticles ◽  
Viral Gene ◽  
Gene Vector ◽  
Inorganic Hybrid ◽  
Sol Gel Process ◽  
Gene Vectors

Novel hybrid biomaterial of gelatin-siloxane nanoparticles (GS NPs), with positive surface potential and lower cytotoxicity, was synthesized through a 2-step sol-gel process. The pDNA-GS NPs complex was formulated with high encapsulation efficiency, and exhibited and efficient transfection in vitro. We thus envision that the GS NPs material could serve as non-viral gene vectors for gene therapy.

In Vitro Cytotoxicity Evaluation of Oxytetracycline Loaded Cockle Shell Derived Calcium Carbonate Aragonite Nanoparticles

2020 ◽  
Vol 10 ◽  
Author(s):  
Sherifat Banke Idris ◽  
Abdul Kadir Arifah ◽  
Faez Firdaus Abdullah Jesse ◽  
Siti Zubaidah Ramanoon ◽  
Muhammad Abdul Basit ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Cell Viability ◽  
Trypan Blue ◽  
Pharmaceutical Formulations ◽  
In Vitro Cytotoxicity ◽  
Mouse Fibroblast ◽  
Nih3t3 Cells ◽  
Toxicological Assessment ◽  
Culture Models

Background: Evaluation of the toxic effects of nanoparticle-drug in vitro is an important step in the design of new pharmaceutical formulations. Rapid results, reduced cost and easy handling makes cell culture models first line in initial toxicological assessment of nanodrug preparations. Objective: To evaluate the in vitro cytotoxicity of oxytetracycline loaded calcium carbonate aragonite nanoparticle in normal mouse fibroblast (NIH3T3) cell line. Method: NIH3T3 cells were exposed to varying concentrations (6.25 - 100µg/mL) of calcium carbonate aragonite nanoparticle (CS-CaCO3NP), oxytetracycline loaded calcium carbonate aragonite nanoparticle (OTC-CS-CaCO3NP) and oxytetracycline (OTC) in 96 well plates for 24, 48 and 72 hours. Cell viability was determined by MTT and trypan blue assays. Result: Both assays show that CS-CaCO3NP and OTC-CS-CaCO3NP had higher cell viability values compared to OTC. Conclusion: Encapsulating OTC into CS-CaCO3NP reduced its cytotoxicity to NIH3T3 cells using both MTT and trypan blue assay.

Drug Loaded Porous Hydroxyapatite and Its In Vitro Release

2005 ◽  
Vol 284-286 ◽  
pp. 423-426 ◽  
Author(s):  
Y. Kim ◽  
B.G. Song ◽  
Soo Ryong Kim ◽  
Kwang Jin Kim
Keyword(s):  
Mesoporous Silica ◽  
Body Fluid ◽  
Sol Gel ◽  
In Vitro Release ◽  
Controlled Drug Delivery ◽  
Porous Hydroxyapatite ◽  
Sol Gel Process ◽  
The Matrix ◽  
Vitro Release

Porous hydroxyapatite coated with mesoporous silica has been utilized as the matrix for controlled drug delivery. TEM observation confirms the pore size of mesoporous silica scatters about 50 Å. Porous hydroxyapatite was coated with mesoporous silica via sol-gel process. Ibuprofen and was loaded into the pores of mesoporous silica, and controlled release profiles were studied by soaking the samples in a simulated body fluid using a UV-VIS spectrophotometer.

Mesoporous 45S5 bioactive glass: synthesis, in vitro dissolution and biomineralization behavior

2017 ◽  
Vol 5 (44) ◽  
pp. 8786-8798 ◽  
Author(s):  
Anil Kumar ◽  
Sevi Murugavel ◽  
Anusha Aditya ◽  
Aldo R. Boccaccini
Keyword(s):  
Bioactive Glass ◽  
Sol Gel ◽  
Sodium Oxide ◽  
In Vitro Dissolution ◽  
Established Method ◽  
Sol Gel Process ◽  
New Generation

The development of a new generation of biomaterials includes a sol–gel process to obtain glass foams, which is a well established method for CaO–SiO2–P2O5 compositions, but is not yet recognized for Bioglass® containing sodium oxide.

scholarly journals STUDY ON THE INFLAMMATORY RESPONSE OF PMMA/POLYSTYRENE/SILICA NANOCOMPOSITE MEMBRANES FOR DRUG DELIVERY AND DENTAL APPLICATIONS

2018 ◽  
Author(s):  
Shanmuga Sundar Saravanabhavan ◽  
KANNAN NATARAJAN ◽  
Sundaravadivel Elumalai ◽  
Sarang Zsolt ◽  
Mukunthan K SELVAM ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Sol Gel ◽  
Research Work ◽  
Polymeric Materials ◽  
Composite Membranes ◽  
In Vitro Cytotoxicity ◽  
Sem Images ◽  
Equal Concentration ◽  
Cytotoxicity Studies

Background The application of polymeric materials in medical industry has grown drastically in the last two decades due to their various advantages compared to existing materials. The present research work emphases on the sol-gel technique to formulate the polymethyl methyl acrylate/polystyrene/silica composite membrane. Methods The characteristic of the composite was investigated through modern state art of instrumentation. Results The functional groups attached to the polymer was absorbed by FTIR. The FTIR spectrum confirm that the blend was mixed thoroughly and the formation of unite intimately between the polymers. The membranes were observed by SEM for its surface homogeneity which depends upon the composition of the two blending polymers. The captured SEM images showed the formation of microcracks on the surface, which was evidently controlled by varying the constituent polymer ratios. The prepared blend membranes with 2:1 ratio of PMMA/PS/Si displayed higher water uptake compared to other blended membranes. The composite membranes had good hydroxyl apatite growth in SBF solution. Furthermore, the in vitro cytotoxicity studies carried out by MTT method, using RAW macrophage cells showed that all the samples exhibited excellent cell viability. Conclusion The inflammatory response of composite with equal concentration of PMMA-PS were performed and observed no inflammation in comparison with control and other tested concentrations.

Macroporous Hydroxyapatite Scaffold Fabricated by Foam Impregnation

2005 ◽  
Vol 288-289 ◽  
pp. 565-570 ◽  
Author(s):  
Y.S. Dong ◽  
B. Liu ◽  
Ping Hua Lin ◽  
Q.G. Zhang ◽  
Yong Ping Pu
Keyword(s):  
Sol Gel ◽  
Ceramic Surface ◽  
Ceramic Slurry ◽  
Hydroxyapatite Powder ◽  
Sol Gel Process ◽  
Aluminum Phosphates ◽  
Good Biocompatibility ◽  
Hydroxyapatite Scaffold ◽  
Sem Analyses

Submicron hydroxyapatite powder with particle size in the range of 80-250 nm was fabricated by sol-gel process in our laboratory. To make ceramic slurry with good flowability, the powder was mixed with binder and distilled water. The binder was consisted of acid magnesium and aluminum phosphates. The polyurethane foam was impregnated in the slurry, squeezed out the excess slurry and the composite porous body gained. Green body was dried in room temperature naturally and then moved to electric furnace and sintered at high temperature. The sintered scaffolds possess interconnected open pore structure and with a porosity of 70-85% and compressive strength 10-20 MPa, and was consisted of doped HA and other phosphates. The scaffolds were co-cultured with osteoblasts in vitro. SEM analyses revealed that the cells adhere to the ceramic surface, proliferate and growth properly. Experimental results showed that the scaffold possesses good biocompatibility and could be used in bone tissue engineering.

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