Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants

2018 ◽  
Vol 97 (9) ◽  
pp. 1003-1009 ◽  
Author(s):  
R.B.P. Miranda ◽  
L. Grenho ◽  
A. Carvalho ◽  
M.H. Fernandes ◽  
F.J. Monteiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Metabolic Activity ◽  
Tetragonal Zirconia ◽  
Likelihood Method ◽  
Biological Behavior ◽  
Cell Metabolic Activity ◽  
Mg63 Cells ◽  
Significant Difference ◽  
Scanning Electron

This investigation aimed at developing micropatterned silica thin films (MSTFs) containing nanohydroxyapatite (nano-HA) microaggregates that were not completely covered by silica so that they could directly interact with the surrounding cells. The objectives were 1) to evaluate the effect of the presence of 2 films (MSTF with or without nano-HA addition) on the characteristic strength (σ0) and Weibull modulus ( m) of a yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) and 2) to evaluate the effect of these 2 films, as applied onto the Y-TZP surface, on the morphology, orientation, and proliferation of MG63 cells. Sol-gel process and soft lithography were used to apply the MSTF onto the Y-TZP specimens. Three experimental groups were produced: Y-TZP, Y-TZP + MSTF, and Y-TZP + MSTF + sprayed nano-HA. All surfaces were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy and tested for 4-point flexural strength ( n = 30) in water at 37 °C. Weibull analysis was used to determine m and σ0 (maximum likelihood method). In vitro biological behavior was performed with human osteoblast-like cells (MG63). Y-TZP was successfully coated with MSFT and MSFT + nano-HA. Scanning electron microscopy micrographs indicated that the microaggregates of nano-HA were not entirely covered by the silica. There was no statistically significant difference among the experimental groups for σ0 and m. In the groups containing the films, the cells were elongated and aligned along the lines. The MSFT + nano-HA group showed significantly higher cell metabolic activity than that obtained for the Y-TZP group at day 7. This investigation was successful in producing an MSTF containing nano-HA microaggregates that remained exposed to the environment. The developed films did not jeopardize the structural reliability of a commercial Y-TZP, as confirmed by the Weibull statistics. The MG63 cells seeded over the films became elongated and aligned along the films’ micropatterned lines. Y-TZP specimens coated with MSTF and nano-HA showed a higher cell metabolic activity and proliferation after 7 d of culture when compared with uncoated Y-TZP.

Fibroblasts During Hypertrophic Scar Formation and Resolution

1973 ◽  
Vol 31 ◽  
pp. 428-429
Author(s):  
C. W. Kischer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Fine Structure ◽  
Metabolic Activity ◽  
Hypertrophic Scar ◽  
Normal Skin ◽  
Ground Substance ◽  
Hypertrophic Scars ◽  
Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

scholarly journals Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Daniele Tognetto ◽  
Chiara De Giacinto ◽  
Alberto Armando Perrotta ◽  
Tommaso Candian ◽  
Alessandro Bova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Femtosecond Laser ◽  
Comparative Study ◽  
Surface Irregularity ◽  
Significant Difference ◽  
Group 2 ◽  
Cut Surface ◽  
Scanning Electron ◽  
Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

scholarly journals Effects of Long Durations of RF–Magnetron Sputtering Deposition of Hydroxyapatite on Titanium Dental Implants

2021 ◽  
Author(s):  
Ihab Nabeel Safi ◽  
Basima Mohammed Ali Hussein ◽  
Hikmat J. Aljudy ◽  
Mustafa S. Tukmachi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Magnetron Sputtering ◽  
Dental Implants ◽  
Substrate Surface ◽  
Rf Magnetron Sputtering ◽  
P Value ◽  
Edx Analysis ◽  
Significant Difference ◽  
Scanning Electron

Abstract Objectives Dental implant is a revolution in dentistry; some shortages are still a focus of research. This study use long duration of radiofrequency (RF)–magnetron sputtering to coat titanium (Ti) implant with hydroxyapatite (HA) to obtain a uniform, strongly adhered in a few micrometers in thickness. Materials and Methods Two types of substrates: discs and root form cylinders were prepared using a grade 1 commercially pure (CP) Ti rod. A RF–magnetron sputtering device was used to coat specimens with HA. Magnetron sputtering was set at 150 W for 22 hours at 100°C under continuous argon gas flow and substrate rotation at 10 rpm. Coat properties were evaluated via field emission scanning electron microscopy (FESEM), scanning electron microscopy–energy dispersive X-ray (EDX) analysis, atomic force microscopy, and Vickers hardness (VH). Student’s t-test was used. Results All FESEM images showed a homogeneous, continuous, and crack-free HA coat with a rough surface. EDX analysis revealed inclusion of HA particles within the substrate surface in a calcium (Ca)/phosphorus (P) ratio (16.58/11.31) close to that of HA. Elemental and EDX analyses showed Ca, Ti, P, and oxygen within Ti. The FESEM views at a cross-section of the substrate showed an average of 7 µm coat thickness. Moreover, these images revealed a dense, compact, and uniform continuous adhesion between the coat layer and the substrate. Roughness result indicated highly significant difference between uncoated Ti and HA coat (p-value < 0.05). A significant improvement in the VH value was observed when coat hardness was compared with the Ti substrate hardness (p-value < 0.05). Conclusion Prolonged magnetron sputtering successfully coat Ti dental implants with HA in micrometers thickness which is well adhered essentially in excellent osseointegration.

Microleakage Evaluation of Composite Restorations Following Papain-Based Chemo-Mechanical Caries Removal in Primary Teeth

2017 ◽  
Vol 41 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Marwa Abdel Hafez ◽  
Mona Elkateb ◽  
Sonia El Shabrawy ◽  
Amel Mahmoud ◽  
Omar El Meligy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopy Study ◽  
Hybrid Layer ◽  
Composite Restorations ◽  
Nail Polish ◽  
Significant Difference ◽  
Drilling Method ◽  
Caries Removal ◽  
Scanning Electron

Aim: To evaluate the microleakage of composite restorations following Papain-based chemo-mechanical caries removal compared to the conventional drilling method. The characteristic of the hybrid layer was also studied using scanning electron microscopy. Study design: The sample included thirty freshly extracted and exfoliated primary molars with open proximal carious dentin lesions. Teeth were divided into two equal groups, according to method of caries removal. Following caries removal, cavity preparations were restored with composite resin. After thermocycling, teeth were sealed apically and coated with nail polish except the surface of restorations and the surrounding 1mm. Teeth were immersed in basic fuschin dye solution, then they were sectioned mesiodistally. The extent of dye penetration was detected using a light stereomicroscope. After microleakage test, the resin/dentin interface was examined using scanning electron microscopy. Results: There was no significant difference in the degree of leakage between both groups. In the Papacarie group, longer and numerous resin tags were observed with statistically significant thicker hybrid layer than those following the drilling method. However, there was no significant difference between the diameters of resin tags of both groups. Conclusions: Papacarie does not adversely affect the microleakage of composite restorations and provides a suitable surface for bonding.

scholarly journals Prosthetic laboratory influence on the vertical misfit at the implant/UCLA abutment interface

2007 ◽  
Vol 18 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Gustavo Augusto Seabra Barbosa ◽  
Paulo Cézar Simamoto Júnior ◽  
Alfredo Júlio Fernandes Neto ◽  
Maria da Glória Chiarello de Mattos ◽  
Flávio Domingues das Neves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Casting Process ◽  
Significant Difference ◽  
Scanning Electron

An inadequate fit at the abutment/implant interface can generate mechanical and biological problems. The laboratorial stages could induce misfit in such interface when the castable UCLA abutment type is used. The purpose of this study was to comparatively evaluate the performance of three prosthetic laboratories (Labs A, B and C) by vertical fit analysis of castable UCLA abutments on the casting and soldering stages of the same prosthesis. Four fixed prostheses were built by each laboratory using castable UCLA abutments. The evaluation was made by scanning electron microscopy under 500x magnification in the mesial and distal regions of each element of the prosthesis, totalizing 24 measurements per laboratory. The results were analyzed statistically by Kruskal-Wallis test (p<0.05). In the casting process, the values presented by the laboratories differed significantly to each other (p=0.004). After soldering, the values presented by the laboratories showed no significant difference (p=0.948). It may be concluded that the fit values obtained in the casting stage of UCLA abutments can be influenced when processed by different laboratories, and that conventional soldering itself increased the degree of framework misfit, regardless of which laboratory made it.

Problems associated with ageing squid from their statoliths: towards a more structured approach

1994 ◽  
Vol 6 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Marek R. Lipinski ◽  
M. Deon Durholtz
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Regression Analysis ◽  
Light Microscopy ◽  
The Other ◽  
Etching Solution ◽  
Significant Difference ◽  
Structured Approach ◽  
Fish Otoliths ◽  
Scanning Electron

It appears that squid statoliths cannot yet be regarded as accurate an ageing tool as fish otoliths. Statoliths from the same pair, prepared differently for viewing and counting increments, were compared. Increment counts do not imply age in days, because this was not validated. One statolith from each pair was examined by light microscopy (LM) after preparation following a new method. The other was viewed by Scanning Electron microscopy (SEM) with a modified etching solution. Shape of each statolith was similar when compared by multiple regression analysis (11 variables, n=53). There was a weak but significant difference between sexes (statoliths of females were slightly larger). All other differences were insignificant. Microscopic observation and increment counts of increments were successfully carried out for 37 pairs of statoliths. Significant differences between two independent counts were found for the LM method, but no significant differences were found between two independent SEM counts. Counts were significantly different when interpreted by both LM and SEM, probably because of poor resolution in the LM readings and over-resolution (growth layers prominent and numerous) in those read by SEM. Recommendations are made on how ageing studies, based on statoliths, should be structured and the results evaluated.

The Distribution of Diatom in the Five Lakes along the Yangtze River

2013 ◽  
Vol 850-851 ◽  
pp. 1309-1312
Author(s):  
Yu Zhou ◽  
Quan Yu ◽  
Wei Hang ◽  
Yu Zhong Wang ◽  
Hui Pin Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Water Samples ◽  
Yangtze River ◽  
Water Bodies ◽  
The Yangtze River ◽  
Significant Difference ◽  
The Future ◽  
Scanning Electron

In order to investigate the diatom distribution in the five lakes along the Yangtze River, and discuss the significance for the diagnosis of drowning. 6 water samples were collected in the five lakes along Yangtze River in June, 2012. Subsequently, The genus and the quantity of the diatoms in the water samples were investigated using scanning electron microscopy (SEM). The result showed us that there was significant difference between the waters sampled from the different sites . These data on diatom distribution are helpful to the diagnosis of the cause of the death as well as to the determination of the drowning site in cases of drowning. Therefore, it is necessary to develop the database of diatoms in more extensive water bodies for the forensic diagnosis of drowning in the future.

scholarly journals Comparison of the Enamel Surface Roughness from Different Polishing Methods: Scanning Electron Microscopy and Atomic Force Microscopy Investigation

2020 ◽  
Vol 14 (02) ◽  
pp. 299-305
Author(s):  
Kiatanan Sugsompian ◽  
Ratchawan Tansalarak ◽  
Thosapol Piyapattamin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Enamel Surface ◽  
Similar Data ◽  
Force Microscopy ◽  
Atomic Force ◽  
Significant Difference ◽  
Scanning Electron

Abstract Objective This study aimed to compare the enamel surface roughness created by four polishing methods after debonding, by using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Materials and Methods Four experimental polishing groups (Sof-Lex disc, SD; sandblaster, SB; tungsten carbide bur, TB; and white stone bur, WB) and one control group were selected from 100 premolars (n = 20/group). The experimental teeth were bonded with a bracket, thermocycled, and debonded. Residual adhesive was removed by either of the respective methods. Pre and postdebonding root mean square (Rq) values were obtained from AFM evaluations. All specimens were examined and evaluated with SEM using a modified enamel surface index (modified ESI). Statistical Analysis Differences among the polishing methods were compared with analysis of variance and Fisher’s least significant difference test at p < 0.05. Results Both microscopic evaluations indicated that the surface with the greatest roughness herein belonged to the SD group, followed by that for SB, TB, and WB groups. AFM measurements indicated a maximum postdebonding Rq herein for the WB group and a significantly greater surface roughness for the TB and WB groups than for the SD and SB groups. Among the experimental groups, SEM followed by modified ESI evaluations revealed similar data to those obtained with AFM. Significant differences were seen among all paired groups, except for that between the SB and TB groups. Conclusion Within the limitations of this study, all four polishing methods were concluded to be clinically acceptable for removing residual orthodontic adhesives.

scholarly journals Residual smear layer after root canal instrumentation by using Niti, M-Wire and CM-Wire instruments: A scanning electron microscopy analysis

2018 ◽  
Vol 12 (03) ◽  
pp. 403-409 ◽  
Author(s):  
Ricardo Machado ◽  
Daniel Comparin ◽  
Eduardo Donato Eing Engelke Back ◽  
Lucas da Fonseca Roberti Garcia ◽  
Luiz Rômulo Alberton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Significant Difference ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Root Canal Instrumentation ◽  
Reciprocating Systems ◽  
Scanning Electron

ABSTRACT Objective: The purpose of this study was to compare the amount of residual smear layer after root canal instrumentation by using Ni-Ti, M-Wire, and CM-Wire instruments. Materials and Methods: Seventy-two mandibular incisors were randomly divided into six groups according to the system used: WaveOne (WO), Reciproc (RP), Unicone (UC), ProTaper Next (PN), Mtwo (MT), and HyFlex (HF). Afterward, the specimens were cleaved in the mesiodistal and buccolingual direction for analysis by scanning electron microscopy. Results: Considering both directions and root canal thirds, there was no difference between HF, MT, and PN. RP, UC, and WO presented a significant difference between the directions, and the cervical third showed a significantly smaller quantity of residual smear layer compared with the apical third. When the systems were compared among them, there was a significant difference only between RP and WO. Conclusions: Residual smear layer observed after instrumentation with the different systems was similar, except for quantities between the reciprocating systems.

scholarly journals Scanning Electron Microscopy Analysis of Changes of Hydroxiapatite/Poly-L-Lactide with Different Molecular Weight of PLLAaAfter Intraperitoneal Implantation

2016 ◽  
Vol 66 (2) ◽  
pp. 234-244
Author(s):  
Ljubiša Đorđević ◽  
Stevo Najman ◽  
Perica Vasiljević ◽  
Miroslav Miljković ◽  
Nenad Ignjatović ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Scanning Electron Microscopy ◽  
Bone Tissue ◽  
Tissue Loss ◽  
Practical Applications ◽  
Molecular Weights ◽  
Significant Difference ◽  
Tissue Reactions ◽  
Scanning Electron

Abstract Implantation of a biomaterial is one of the important trends in solving the problem of bone tissue loss. Calcium hydroxiapatite (HAp), as the most representative bone component is a serious candidate for such implantations. The synthetic polymer poly-L-lactide (PLLA) in HAp/PLLA is often used as a polymeric material, with a role in the substitution of bone tissue collagen fibers. Fibers of PLLA may strengthen HAp and its good bioresorption provides space for tissue remodeling. Differences in porosity, microstructure, compressive consistency as well as bioresorbility of HAp/ PLLA may be achieved by using PLLA with different molecular weights. In this study HAp/PLLA composites with PLLA of different molecular weights (50,000; 160,000 and 430,000) were implanted in mouse peritoneum in order to examine the influence of the molecular weight of PLLA on morphology changes. Microstructural changes of biomaterial (HAp/PLLA) surface were analyzed one week, three weeks and four months after their implantation using Scanning Electron Microscopy. The results showed a significant difference in tissue reactions on the applied biocomposites, depending on their molecular weight. The most intense proliferation of cells was induced by HAp/PLLA 50,000 compared to HAp/PLLA 430,000 and HAp/PLLA 160,000. In the vicinity of HAp/PLLA 430,000 abundant erythrocytes were observed. The differences in biological reactions on the examined biocomposites are significant for their practical applications. HAp/PLLA composite biomaterials of different types and resorption rates require specific designing and programming to become suitable for particular purposes in an organism.

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