scholarly journals Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Daniele Tognetto ◽  
Chiara De Giacinto ◽  
Alberto Armando Perrotta ◽  
Tommaso Candian ◽  
Alessandro Bova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Femtosecond Laser ◽  
Comparative Study ◽  
Surface Irregularity ◽  
Significant Difference ◽  
Group 2 ◽  
Cut Surface ◽  
Scanning Electron ◽  
Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

scholarly journals Efficacy of Two Irrigants Used with Self-Adjusting File System on Smear Layer: A Scanning Electron Microscopy Study

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Özgür Genç Şen ◽  
Sadullah Kaya ◽  
Özgür Er ◽  
Tayfun Alaçam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Citric Acid ◽  
Evaluation System ◽  
Smear Layer ◽  
Root Canals ◽  
Significant Difference ◽  
Group 2 ◽  
Scanning Electron ◽  
Group 1

Mechanical instrumentation of root canals produces a smear layer that adversely affects the root canal seal. The aim of this study was to evaluate efficacy of MTAD and citric acid solutions used with self-adjusting file (SAF) system on smear layer. Twenty-three single-rooted human teeth were used for the study. Canals were instrumented manually up to a number 20 K file size. SAF was used to prepare the root canals. The following groups were studied: Group 1: MTAD + 5.25% NaOCl, Group 2: 20% citric acid + 5.25% NaOCl, and Group 3: Control (5.25% NaOCl). All roots were split longitudinally and subjected to scanning electron microscopy. The presence of smear layer in the coronal, middle, and apical thirds was evaluated using a five-score evaluation system. Kruskal-Wallis and Mann-Whitney U tests were used for statistical analysis. In the coronal third, Group 2 exhibited the best results and was statistically different froms the other groups (P<0.05). There was not a significant difference among the three thirds of groups according to in-group comparisons (P>0.05). The solutions used in Group 1 and 2 could effectively remove smear layer in most of the specimens. However, citric acid was more effective than MTAD in the three thirds of the canal.

scholarly journals Resistance to Dislodgement of REBILDA® Post System to Intraradicular Dentin

2017 ◽  
Vol 19 (2) ◽  
pp. 47
Author(s):  
Daphne Ross DDS ◽  
Carlos E. Filloy Esna DDS ◽  
Juan Carlos Ramírez-Barrantes DDS, MSD, PhD
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bond Strength ◽  
Adhesive Systems ◽  
Root Reinforcement ◽  
Significant Difference ◽  
Group 2 ◽  
Push Out ◽  
Scanning Electron ◽  
Group 1

Currently there is controversy in the literature about clinical and adhesive effectiveness with respect to adhesive systems aimed to  root reinforcement. Thus, the objective of this study was to evaluate the Push-Out bond strength between the Rebilda® post system and the intraradicular dentine. Thus, 28 unirradicular teeth were used, cut and standardized in 16 mm. The root segments were submitted to endodontic treatment; and then randomly divided into two groups, Group 1 (n = 14): Rebilda® DC + Futurabond DC; Group 2 (n = 14): RelyX ARC + Adper Scothbond Multipurpose Plus. Ten specimens from each group were submitted  to the Push-Out Test. The remaining 4 specimens from each group were prepared for Scanning Electron Microscopy (SEM) in order to evaluate the formation of resinous extensions at the post-cement-dentin interface. Group 2 showed better results when compared to Group 1 regarding bond strength and in formation of resinous extensions or tags, but there was no statistically significant difference (p>0.05). It was concluded that the REBILDA® Post System promotes adequate bond strength to intraradicular dentin.

Scanning electron microscopy evaluation of the effect of etching agents on human enamel surface

2006 ◽  
Vol 30 (3) ◽  
pp. 247-250 ◽  
Author(s):  
Caio Zanet ◽  
Victor Arana-Chavez ◽  
Marcelo Fava
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phosphoric Acid ◽  
Enamel Surface ◽  
Human Enamel ◽  
Group 3 ◽  
Group 2 ◽  
Scanning Electron ◽  
Adhesive Materials ◽  
Group 1

Acid etching promotes microporosities on enamel surface, which provide a better bonding surface to adhesive materials. The purpose of this study was to comparatively analyze the microstructure of enamel surface after etching with 37% phosphoric acid or with two self-etching primers, Non-rinse conditioner (NRC) and Clearfil SE Bond (CSEB) using scanning electron microscopy. Thirty sound premolars were divided into 3 groups with ten teeth each: Group 1: the buccal surface was etched with 37% phosphoric acid for 15 seconds; Group 2: the buccal surface was etched with NRC for 20 seconds; Group 3: the buccal surface was etched with CSEB for 20 seconds. Teeth from Group 1 were rinsed with water; teeth from all groups were air-dried for 15 seconds. After that, all specimens were processed for scanning electron microscopy and analyzed in a Jeol 6100 SEM. The results showed deeper etching when the enamel surface was etched with 37% phosphoric acid, followed by NRC and CSEB. It is concluded that 37% phosphoric acid is still the best agent for a most effective enamel etching.

scholarly journals EFFICACY OF TWO PIEZOELECTRIC ULTRASONIC SCALERS WITH HAND INSTRUMENTATION: A PROFILOMETRIC AND SEM STUDY

2019 ◽  
Vol 3 (4) ◽  
Author(s):  
Dr. Sruthy Noble ◽  
Dr. Nisha K. J. ◽  
Dr. Parichaya Batra ◽  
Dr. Shyam Padmanabhan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Roughness ◽  
Root Surface ◽  
P Value ◽  
Root Planing ◽  
Significant Difference ◽  
Group 2 ◽  
Hand Instrumentation ◽  
Scanning Electron

Background: The goal of the present study was to compare the efficacy of two piezoelectric scalers with hand instrumentation in calculus removal, loss of tooth substance and roughness created on root surface. Methods: 90 freshly extracted non-carious, non-restored single rooted teeth with hopeless periodontal prognosis were included in the study. They were divided into three groups of 30 teeth each i.e. Group 1,Group 2 and Group 3 where instrumentation was done using two piezoelectric scalers- Piezoelectric scaler A & Piezoelectric scaler B and Gracey curette respectively. Specimens were subjected to Scanning Electron Microscopy (SEM) & Profilometric analysis to evaluate the root surface roughness created by these three instruments. Results: Statistically significant difference was observed in Remaining calculus index (RCI) in the three experimental groups with hand instrument being least effective in removing calculus when compared to the piezoelectric scalers(p≤0.05). There was significant difference in mean Loss of tooth substance index(LTSI) and Roughness loss of tooth substance index (RLTSI) scores when compared in the three groups (p value =0.002). Both SEM and profilometric analysis showed that Piezoelectric scaler A and Piezoelectric scaler B caused less root surface roughness compared to hand instruments. Conclusion: The findings of the present study suggest that scaling and root planing using both piezoelectric scalers and hand instruments were effective in mechanical debridement of the root surface. However, piezoelectric instruments gave smoother root surface than hand instruments and were also more efficient in calculus removal. Key words: Dental scaling, Root planing, Ultrasonics, Scanning Electron Microscopy, Dental calculus

scholarly journals Effects of Long Durations of RF–Magnetron Sputtering Deposition of Hydroxyapatite on Titanium Dental Implants

2021 ◽  
Author(s):  
Ihab Nabeel Safi ◽  
Basima Mohammed Ali Hussein ◽  
Hikmat J. Aljudy ◽  
Mustafa S. Tukmachi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Magnetron Sputtering ◽  
Dental Implants ◽  
Substrate Surface ◽  
Rf Magnetron Sputtering ◽  
P Value ◽  
Edx Analysis ◽  
Significant Difference ◽  
Scanning Electron

Abstract Objectives Dental implant is a revolution in dentistry; some shortages are still a focus of research. This study use long duration of radiofrequency (RF)–magnetron sputtering to coat titanium (Ti) implant with hydroxyapatite (HA) to obtain a uniform, strongly adhered in a few micrometers in thickness. Materials and Methods Two types of substrates: discs and root form cylinders were prepared using a grade 1 commercially pure (CP) Ti rod. A RF–magnetron sputtering device was used to coat specimens with HA. Magnetron sputtering was set at 150 W for 22 hours at 100°C under continuous argon gas flow and substrate rotation at 10 rpm. Coat properties were evaluated via field emission scanning electron microscopy (FESEM), scanning electron microscopy–energy dispersive X-ray (EDX) analysis, atomic force microscopy, and Vickers hardness (VH). Student’s t-test was used. Results All FESEM images showed a homogeneous, continuous, and crack-free HA coat with a rough surface. EDX analysis revealed inclusion of HA particles within the substrate surface in a calcium (Ca)/phosphorus (P) ratio (16.58/11.31) close to that of HA. Elemental and EDX analyses showed Ca, Ti, P, and oxygen within Ti. The FESEM views at a cross-section of the substrate showed an average of 7 µm coat thickness. Moreover, these images revealed a dense, compact, and uniform continuous adhesion between the coat layer and the substrate. Roughness result indicated highly significant difference between uncoated Ti and HA coat (p-value < 0.05). A significant improvement in the VH value was observed when coat hardness was compared with the Ti substrate hardness (p-value < 0.05). Conclusion Prolonged magnetron sputtering successfully coat Ti dental implants with HA in micrometers thickness which is well adhered essentially in excellent osseointegration.

A Comparative Study of Trichomes in Angophora Cav. And Eucalyptus L'hérit. - A Question of Homology

1984 ◽  
Vol 32 (5) ◽  
pp. 561 ◽  
Author(s):  
PY Ladiges
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Study ◽  
Light Microscopy ◽  
Evolutionary Trends ◽  
Oil Glands ◽  
Ancestral Condition ◽  
Scanning Electron

The trichomes of Angophora and Eucalyptus are illustrated from scanning electron microscopy and light microscopy, and evolutionary trends are discussed. Bristle glands of Angophora and Eucalyptus subgen. Blakella and Corymbia are emergent oil glands of varying lengths. Emergent oil glands occur in all other Eucalyptus subgenera but they are most conspicuous in Blakella, Corymbia and Angophora, in which they are characterized by four cap cells each ornamented with micropapillae. Hairs in Angophora are unique, being multicellular; they are also uniseriate and scattered on the epidermis. In contrast, hairs in Eucalyptus are simple extensions, short or long, of the cells on the sides of or the cap cells of the emergent oil glands, and they are not homologous with those of Angophora. Eucalyptus setosa (subgen. Blakella) and E. brockwayi (subgen. Symphyomyrtus) are two exceptions, having unicellular hairs on the epidermis, not associated with oil glands. It is suggested that this is an ancestral condition (or secondary reversal to it).

Microleakage Evaluation of Composite Restorations Following Papain-Based Chemo-Mechanical Caries Removal in Primary Teeth

2017 ◽  
Vol 41 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Marwa Abdel Hafez ◽  
Mona Elkateb ◽  
Sonia El Shabrawy ◽  
Amel Mahmoud ◽  
Omar El Meligy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopy Study ◽  
Hybrid Layer ◽  
Composite Restorations ◽  
Nail Polish ◽  
Significant Difference ◽  
Drilling Method ◽  
Caries Removal ◽  
Scanning Electron

Aim: To evaluate the microleakage of composite restorations following Papain-based chemo-mechanical caries removal compared to the conventional drilling method. The characteristic of the hybrid layer was also studied using scanning electron microscopy. Study design: The sample included thirty freshly extracted and exfoliated primary molars with open proximal carious dentin lesions. Teeth were divided into two equal groups, according to method of caries removal. Following caries removal, cavity preparations were restored with composite resin. After thermocycling, teeth were sealed apically and coated with nail polish except the surface of restorations and the surrounding 1mm. Teeth were immersed in basic fuschin dye solution, then they were sectioned mesiodistally. The extent of dye penetration was detected using a light stereomicroscope. After microleakage test, the resin/dentin interface was examined using scanning electron microscopy. Results: There was no significant difference in the degree of leakage between both groups. In the Papacarie group, longer and numerous resin tags were observed with statistically significant thicker hybrid layer than those following the drilling method. However, there was no significant difference between the diameters of resin tags of both groups. Conclusions: Papacarie does not adversely affect the microleakage of composite restorations and provides a suitable surface for bonding.

Comparative Study of Field Emission-Scanning Electron Microscopy and Atomic Force Microscopy to Assess Self-assembled Monolayer Coverage on Any Type of Substrate

1999 ◽  
Vol 5 (6) ◽  
pp. 413-419 ◽  
Author(s):  
Bernardo R.A. Neves ◽  
Michael E. Salmon ◽  
Phillip E. Russell ◽  
E. Barry Troughton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Comparative Study ◽  
Field Emission ◽  
Self Assembled Monolayer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Self Assembled ◽  
Scanning Electron

Abstract: In this work, we show how field emission–scanning electron microscopy (FE-SEM) can be a useful tool for the study of self-assembled monolayer systems. We have carried out a comparative study using FE-SEM and atomic force microscopy (AFM) to assess the morphology and coverage of self-assembled monolayers (SAM) on different substrates. The results show that FE-SEM images present the same qualitative information obtained by AFM images when the SAM is deposited on a smooth substrate (e.g., mica). Further experiments with rough substrates (e.g., Al grains on glass) show that FE-SEM is capable of unambiguously identifying SAMs on any type of substrate, whereas AFM has significant difficulties in identifying SAMs on rough surfaces.

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