Nasal Inflammation and Ulceration Secondary to Repeated Use of an Intranasal Delivery Device in Rabbits

2020 ◽  
Vol 48 (7) ◽  
pp. 909-912
Author(s):  
Yuval Ramot ◽  
Dalia Shabashov Stone ◽  
Ruth Goldschmidt ◽  
Abraham Nyska
Keyword(s):  
Intranasal Delivery ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Delivery Device ◽  
Superficial Ulcer ◽  
Nasal Anatomy ◽  
Repeated Use ◽  
Nasal Inflammation ◽  
Human Use ◽  
Proper Interpretation

Intranasal administration of drugs is gaining popularity in medicine, and several animal models have been used to test the safety and efficacy of this delivery route. Nevertheless, the nasal anatomy of animals is different from humans, which can lead to pathological changes that stem from the delivery device and not the drug itself. Here, we report on nasal inflammation and ulceration in rabbits, secondary to the repeated trauma caused by the intranasal device. Similar changes were noted in the animals treated with the vehicle and with the tested drug, and therefore, these changes were not attributed to the drug itself. In some animals, superficial ulcer and stromal inflammation were noted in the eyes, secondary to nasal duct obstruction from the nasal inflammation. These observations emphasize the importance of proper interpretation of histopathological changes, attributed to trauma-induced pathological changes related to the handling of the animal and not to the tested product, which is the drug itself and the device that is optimized for clinical (human) use.

scholarly journals Histopathological changes in pigs infected with ileitis

2020 ◽  
Vol 10 (4) ◽  
pp. 72-77
Author(s):  
O.M. Yermolenko ◽  
O.Y. Ayshpur ◽  
I.Yu. Mushtuk
Keyword(s):  
Histological Examination ◽  
Severe Disease ◽  
Gastrointestinal Diseases ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Lawsonia Intracellularis ◽  
Early Diagnostics ◽  
Chronic Effect ◽  
Anatomical Changes ◽  
Proliferative Enteropathy

Nowadays, the ileitis (proliferative enteropathy of pigs) is one of the most common gastrointestinal diseases. The intracellular bacterium Lawsonia intracelluaris causes several forms of the disease. Histological examinations are the important stages in diagnosing and detecting profound pathological changes in sick pig organs, decreasing their productivity and causing the deaths. Swine proliferative enteropathy (SPE) has reported for the Ukraine since 2008. For SPE the early diagnostics with different test systems is relevant. We evaluated the sick, forcibly killed and dead piglets of different ages in Ukrainian pig farms during 2018-2019 in animal fattening groups. Materials were taken from piglets aged 120-150 days in farms where the ileitis was diagnosed. To assess the complex of pathological and anatomical changes detected during autopsies of piglets, V.P. Shishkov’s methods were used. Thus, we revealed a characteristic morphology of proliferative lesions. We believed that the histological examination can serve as an important diagnostic method and an alternative to direct isolation of Lawsonia intracellularis, which is very difficult to cultivate. We determined that the histopathological changes in pig organs indicated a chronic effect caused by animal pathogen. We revealed that Lawsonia intracellularis caused the profound pathological changes in pig digestive tracts, which affected the functioning of all organs and tissues of animals and lead to severe disease and possible animal death. We suggested that the histological examination is an important step in diagnosing and detecting profound pathological changes in the organs of sick pigs, which caused the decrease in their productivity and deaths.

scholarly journals Histopathological changes in gallbladder mucosa associated with cholelithiasis

1970 ◽  
Vol 2 (3) ◽  
pp. 224-225 ◽  
Author(s):  
R Baidya ◽  
B Sigdel ◽  
NL Baidya
Keyword(s):  
Retrospective Study ◽  
Intestinal Metaplasia ◽  
Gastrointestinal Disorder ◽  
Gallstone Formation ◽  
Epithelial Hyperplasia ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Gallbladder Epithelium ◽  
Gallbladder Mucosa ◽  
Gross Examination

Background: Gallstone is a very common gastrointestinal disorder. It is known to produce histopathological changes in the gallbladder. It is also one of the predisposing factors for the development of cancer of gallbladder. Materials and Methods: This was a retrospective study carried out in the Department of Histopathology, B and B Hospital during a period of 1 year from April 2010 to May 2011. The study included 396 cases of cholecystectomy specimens. Results: On gross examination, outer gallbladder surface was congested in 116 patients (28%), wall thickness was increased in 181 (45.7%) and mucosal abnormalities were present in 126 (31.6%) patients. At microscopy, epithelial hyperplasia was observed in 183 (46.2%), intestinal metaplasia in 112 (28.2%), dysplasia in 5 (1.3%) and cholesterolosis in 56 (14%) patients. Conclusion: The pathological changes of the gallbladder epithelium may play an important role in the process of gallstone formation. DOI: http://dx.doi.org/10.3126/jpn.v2i3.6027 JPN 2012; 2(3): 224-225

scholarly journals Failure of Added Dietary Gluten to Induce Small Intestinal Histopathological Changes in Patients with Watery Diarrhea and Lymphocytic Colitis

1996 ◽  
Vol 10 (7) ◽  
pp. 436-439 ◽  
Author(s):  
Hugh James Freeman
Keyword(s):  
Celiac Disease ◽  
Small Bowel ◽  
Microscopic Colitis ◽  
Lymphocytic Colitis ◽  
Watery Diarrhea ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Proximal Small Bowel ◽  
Colorectal Biopsies ◽  
Small Intestinal

Lymphocytic colitis is a form of microscopic colitis usually characterized by watery diarrhea and often associated with biopsy-defined celiac disease. Two patients with lymphocytic colitis and normal small intestinal biopsies who were administered 40 g of added dietary gluten for four consecutive weeks are presented. Small intestinal biopsies from multiple sites in the proximal small bowel were done after three and four weeks to determine whether pathological changes in latent celiac disease could be induced in these patients with a high gluten-containing diet. In addition, colorectal biopsies were done to determine whether the colitis was sensitive to oral gluten. No alterations in the small intestinal biopsies were detected in either patient and no changes occurred in colitis severity. Although microscopic forms of colitis have been linked to celiac disease, this study indicates that lymphocytic colitis is a heterogeneous clinicopathological disorder that, in some patients, is independent of any gluten-induced intestinal pathological changes.

scholarly journals Case report: Histopathological Diagnosis of Rabies in Iraqi Cows and Ewes

2011 ◽  
Vol 35 (2) ◽  
Author(s):  
Magda Saeed Abdullah
Keyword(s):  
Case Report ◽  
Blood Vessels ◽  
Purkinje Cells ◽  
Histopathological Diagnosis ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Intracytoplasmic Inclusions ◽  
Cerebral Parenchyma ◽  
Cellular Necrosis ◽  
First Time

Rabies was diagnosed (for the first time) in clinically suspected rabid Iraqi cows and ewes by using histopathological methods. The result showed 3 positive cases 2 cows from Baghdad governorate and 1 ewe from Al - Basra governorate. The gross pathological changes included swelling of cerebrum and cerebellum, multiple hemorrhagic spots within cerebral parenchyma, and sever congestion in meningial blood vessels. The histopathological changes showed specific changes represented by Negri bodies as intracytoplasmic inclusions within Purkinje cells in cerebellum and Babes nodules in cerebrum and medulla oblongata. Non specific changes included edema, hemorrhages, cellular necrosis, lymphocytic foci and lymphocytic and mononuclear cuffing surrounding congested blood vessels.

scholarly journals Histopathological observations in COVID-19: a systematic review

2020 ◽  
pp. jclinpath-2020-206995 ◽  
Author(s):  
Vishwajit Deshmukh ◽  
Rohini Motwani ◽  
Ashutosh Kumar ◽  
Chiman Kumari ◽  
Khursheed Raza
Keyword(s):  
Public Health ◽  
World Health ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Immune Systems ◽  
Search Terms ◽  
The World ◽  
Health Organization ◽  
Global Threat ◽  
Meta Analyses

BackgroundCoronavirus disease-2019 (COVID-19) has caused a great global threat to public health. The World Health Organization (WHO) has declared COVID-19 disease as a pandemic, affecting the human respiratory and other body systems, which urgently demands for better understanding of COVID-19 histopathogenesis.ObjectiveData on pathological changes in different organs are still scarce, thus we aim to review and summarise the latest histopathological changes in different organs observed after autopsy of COVID-19 cases.Materials and methodsOver the period of 3 months, authors performed vast review of the articles. The search engines included were PubMed, Medline (EBSCO & Ovid), Google Scholar, Science Direct, Scopus and Bio-Medical. Search terms used were ‘Histopathology in COVID-19’, ‘COVID-19’, ‘Pathological changes in different organs in COVID-19’ or ‘SARS-CoV-2’. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2009 guidelines were used for review writing.ResultWe identified various articles related to the histopathology of various organs in COVID-19 positive patients. Overall, 45 articles were identified as full articles to be included in our study. Histopathological findings observed are summarised according to the systems involved.ConclusionAlthough COVID-19 mainly affects respiratory and immune systems, but other systems like cardiovascular, urinary, gastrointestinal tract, reproductive system, nervous system and integumentary system are not spared, especially in elderly cases and those with comorbidity. This review would help clinicians and researchers to understand the tissue pathology, which can help in better planning of the management and avoiding future risks.

Evaluating the Laser Output of the Micropulse P3 Laser Delivery Device Following Repeated Use

2019 ◽  
Vol 28 (8) ◽  
pp. 697-700 ◽  
Author(s):  
Katherine Lun ◽  
Maya E. Suwandono ◽  
Victor Koh ◽  
Paul T. Chew
Keyword(s):  
Laser Output ◽  
Delivery Device ◽  
Repeated Use

scholarly journals Study on health status of farmed shing (Heteropneustes fossilis) in Mymensingh region

2020 ◽  
Vol 6 (3) ◽  
pp. 445-455
Author(s):  
Md Asek Uddin ◽  
Md Ariful Islam ◽  
Rashedul Islam ◽  
Rabeya Yesmin ◽  
KM Abdul Halim ◽  
...  
Keyword(s):  
Health Status ◽  
Winter Season ◽  
Clinical Signs ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Heteropneustes Fossilis ◽  
Fish Farms ◽  
Fat Bodies ◽  
Gill Lamellae

Health status investigation of shing (Heteropneustes fossilis) was carried out through clinical and histopathological observations. During four months from January 2018 to April 2018. The data were collected from eight fish farms. Clinical signs of fish were physical deformity, reddish whitish lesions, abrasion and reduced mucus in the months of January and February from the investigated fish farms. Clinically fish were almost normal in the months of March and April. Histopathlogically observed pathological changes in gill were lamellar missing, splitted gill lamellae, hemorrhage, hypertrophy, vaccums and presence of parasites in January and February. In case of liver the examined fish had hemorrhage, vaccums, necrosis and fat bodies also in the months of January and February. Organs of the examined fishes were almost normal in the months of March and April. The purpose of the study was to determine the health status of farmed shing. The main objectives of the study were to observe clinical and histopathological changes in liver and gill of farmed shing (H. fossilis) at various fish farms of Mymensingh region and to determine the health status of the shing in the investigated months. From the present study, it was found that external organs like gill were more affected compared to internal organ like liver. From the present investigation, it was also observed that the fish were more affected in the months of January and February i.e. in winter season. Res. Agric., Livest. Fish.6(3): 445-455, December 2019

scholarly journals Histopathological study for the effect of vitamin C on the some mice tissues treated with nitrate

2007 ◽  
Vol 31 (2) ◽  
pp. 151-158
Author(s):  
R. N. Al-saadi
Keyword(s):  
Vitamin C ◽  
Potassium Nitrate ◽  
The Other ◽  
Histopathological Study ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Other Hand ◽  
Vit C

Thirty two mice were used to study the histopathological changes resultedfrom the effect of vitamin C on the some mice tissues treated with nitrate.Mice were divided randomly in to four equal groups. The 1st group was givenVit C and potassium nitrate via water. The 2nd group was given potassium nitrateand the 3rd group received Vit C only, while the 4th group represented thecontrol.Two animals from each group were scarified weekly. The results showedsimilar pathological changes in the 1st and 2nd group especially in the stomachand intestine which gave severe changes that characterized by hyperplasticchanges in addition for the hemorrhages and the infiltrations of inflammatorycells in all layers of both organs. Less alteration was seen in the liver andkidney. On the other hand, no pathological changes in the 3rd and 4th groupwere recorded

scholarly journals A Model of a Novel Coronavirus Infection in Golden Syrian Hamsters: Major Pathological Changes

Biomeditsina ◽  
2021 ◽  
Vol 17 (3) ◽  
pp. 90-94
Author(s):  
S. V. Chepur ◽  
V. A. Myasnikov ◽  
M. A. Tyunin ◽  
N. S. Ilinskiy ◽  
A. S. Nikishin ◽  
...  
Keyword(s):  
Body Weight ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Syrian Hamsters ◽  
Coronavirus Infection ◽  
Novel Coronavirus

The paper presents data on the dynamics of body weight and mass coeffi cient of lungs in golden Syrian hamsters after infection with the SARS CoV-2 virus. It was found that the most pronounced deviations of the studied parameters develop on the 7th day of the disease. The study also demonstrates the main histopathological changes in the lungs during this period of the disease.

scholarly journals Supplementation of Vitamin E Protects Chickens from Newcastle Disease Virus-Mediated Exacerbation of Intestinal Oxidative Stress and Tissue Damage

2018 ◽  
Vol 47 (4) ◽  
pp. 1655-1666 ◽  
Author(s):  
Zaib Ur Rehman ◽  
Luping Che ◽  
Shanhui Ren ◽  
Ying Liao ◽  
Xusheng Qiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Vitamin E ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Negative Control ◽  
Jejunal Mucosa ◽  
Pathological Changes ◽  
Histopathological Changes

Background/Aims: Newcastle disease virus (NDV) causes a highly devastating and contagious disease in poultry, which is mainly attributed to extensive tissue damages in the digestive, respiratory and nervous systems. However, nature and dynamics of NDV-induced oxidative stresses in the intestine of chickens remain elusive. Methods: In this study, we examined the magnitude of intestinal oxidative stress and histopathological changes caused by the virulent NDV infection, and explored the protective roles of vitamin E (vit. E) in ameliorating these pathological changes. For these purposes, chickens were divided into four groups namely i) non supplemented and non-challenged (negative control, CON); ii) no supplementation of vit. E but challenged with ZJ1 (positive control, NS+CHA); iii) vit. E supplementation at the dose of 50 IU/day/Kg body weight and ZJ1 challenge (VE50+CHA); and 4) vit. E supplementation at the dose of 100 IU/day/Kg body weight and ZJ1 challenge (VE100+CHA). In all groups, we analyzed concentrations of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (T-AOC), and activity of glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) using biochemical methods. The virus loads were determined by quantitative RT-PCR and antibody titers by hemagglutination inhibition assays. We also examined the histopathological changes in the duodenal and jejunal mucosa at 3 and 5-day post infection (dpi) with NDV. Results: A significant elevation in the NO level was observed in NDV challenged chickens compared to the CON chickens at 2 dpi. The MDA contents were significantly increased whereas GSH was significantly decreased in NDV-challenged chickens compared to control. Furthermore, activities of GST, CAT, SOD, as well as the TOAC were markedly decreased in challenged chickens in comparison with control. Virus copy numbers were higher in NDV infected NS+CHA group compared to other groups. Severe histopathological changes including inflammation, degeneration and broken villi were observed in the intestine of NDV challenged chickens. However, all these malfunctions of antioxidant system and pathological changes in the intestine were partially or completely reversed by the vit. E supplementation. Conclusions: Our results suggest that NDV infection causes oxidative stress and histopathological changes in the duodenum and jejunum of chickens, which can be partially or fully ameliorated by supplementation of vit. E. Additionally, these findings suggest that oxidative stress contributes to the intestinal damages in NDV infected chickens. These findings will help to understand the pathogenesis of NDV and further investigation of therapeutic agents for control of Newcastle disease.

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