A Hyperbaric Chamber for Observation of Cell Cultures at Pressure

1987 ◽  
Vol 14 (3) ◽  
pp. 158-160
Author(s):  
J. Jenssen ◽  
G. Bolstad ◽  
T. Syversen
Keyword(s):  
Phase Contrast ◽  
Video Recording ◽  
Time Lapse ◽  
Pressure Effects ◽  
Chamber System ◽  
Hyperbaric Chamber ◽  
Stainless Steel Tubing ◽  
Injection Pump ◽  
Pharmacological Studies ◽  
Steel Tubing

A chamber system has been constructed for the study of hydrostatic and gas pressure effects on cells in culture. The chamber is fitted with a phase contrast light microscope allowing direct observation and video recording of the cells at pressure. Cell differentiation over a period of hours to days may thus be recorded and taped from as many as 24 cell samples in the same experiment, using a time-lapse recording technique. Solutions may be introduced and removed through stainless steel tubing via an injection pump, thus providing the additional possibility of biochemical and pharmacological studies.

Time-lapse Phase-contrast Microphotography of Cell Populations as a Basis for Improvement of In Vitro Toxicity Assessment

1992 ◽  
Vol 20 (2) ◽  
pp. 302-306
Author(s):  
Miroslav Červinka
Keyword(s):  
Cell Proliferation ◽  
Cell Morphology ◽  
Video Recording ◽  
Toxicity Testing ◽  
Time Lapse ◽  
Time Dependent ◽  
In Vitro Toxicity ◽  
Simple Modification ◽  
Pertinent Data

Recent trends in the field of in vitro toxicology have centred around the validation of in vitro methods. The ultimate goal is to obtain pertinent data with the minimum of effort. In our laboratory, we have used toxicological methods based on the evaluation of cell morphology and cell proliferation. A method suitable for this purpose is time-lapse microcinematographic (or video) recording of cellular changes, which we used for many years. For practical in vitro toxicity testing, however, this method is far too complicated. Therefore, we have tried to develop a simple modification for the evaluation of cell morphology and cell proliferation, which would still allow for a basic time-dependent analysis. Comparison of detailed microcinematographic analysis with analysis according to our new proliferation assay is demonstrated with cisplatin as the toxicant. We believe that a time-dependent approach could improve the in vitro assessment of toxicity.

Pulsed eddy-current inspection of thin-walled stainless steel tubing

1989 ◽  
Vol 22 (3) ◽  
pp. 180
Author(s):  
C.V. Dodd ◽  
D.W. Koerner ◽  
W.E. Deeds ◽  
C.A. Pickett
Keyword(s):  
Stainless Steel ◽  
Eddy Current ◽  
Stainless Steel Tubing ◽  
Pulsed Eddy Current ◽  
Eddy Current Inspection ◽  
Steel Tubing ◽  
Thin Walled

Collecting Vehicle-Speed Data by Using Time-Lapse Video Recording Equipment

2003 ◽  
Vol 1855 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Christopher Strong ◽  
Scott Lowry ◽  
Peter McCarthy
Keyword(s):  
Real Time ◽  
Video Recording ◽  
Warning System ◽  
Time Lapse ◽  
Vehicle Speed ◽  
Recording Equipment ◽  
Safety Improvement ◽  
Camera Location ◽  
Message Signs ◽  
Time Lapse Video

An innovative application of time-lapse video recording is used to assist in an evaluation of a highway safety improvement. The improvement is an icy-curve warning system near Fredonyer Summit in northern California that activates real-time motorist warnings via extinguishable message signs, based on weather readings collected from road weather information systems. A measure of effectiveness is whether motorist speed is reduced as a result of real-time warnings to drivers. Why indirect speed measurement with video was preferred over radar for this case is discussed, as is how specific methodological issues related to the custom-built equipment, including camera location and orientation, distance benchmarking, and data collection and reduction. Theoretical and empirical accuracy measurements show that the video surveillance trailers yield results comparable to radar and, hence, would be applicable for studies in which speed change is measured. Because this particular technology had not been used previously, several lessons are documented that may help determine where and how similar equipment may be optimally used in future studies.

Bipolar diathermy forceps with automatic irrigation

1985 ◽  
Vol 62 (6) ◽  
pp. 933-934 ◽  
Author(s):  
Tomio Ohta ◽  
Noboru Funatsu ◽  
Toshihiko Kuroiwa ◽  
Takayoshi Matsui
Keyword(s):  
Stainless Steel ◽  
Bipolar Diathermy ◽  
Content Type ◽  
Stainless Steel Tubing ◽  
Steel Tubing ◽  
Fine Print

✓ A method for providing a saline drip during bipolar diathermy is described. Stainless steel tubing is incorporated in both blades of standard bipolar forceps and connected to the irrigating line. Irrigation is started when the forceps are closed and is stopped when they are open.

Scaled-up self-assembly of carbon nanotubes inside long stainless steel tubing

Carbon ◽  
2006 ◽  
Vol 44 (7) ◽  
pp. 1235-1242 ◽  
Author(s):  
Mahesh Karwa ◽  
Zafar Iqbal ◽  
Somenath Mitra
Keyword(s):  
Carbon Nanotubes ◽  
Stainless Steel ◽  
Self Assembly ◽  
Stainless Steel Tubing ◽  
Steel Tubing

An analysis of in vivo cell migration during teleost fin morphogenesis

1984 ◽  
Vol 66 (1) ◽  
pp. 205-222
Author(s):  
A. Wood ◽  
P. Thorogood
Keyword(s):  
Close Association ◽  
Video Recording ◽  
Time Lapse ◽  
Mesenchymal Cells ◽  
Mean Value ◽  
Distal Margin ◽  
Cellular Processes ◽  
Interference Contrast Microscopy ◽  
Time Lapse Video

In the teleost embryo the pectoral fin bud initially displays an apical ectodermal ridge along its entire distal margin. The ridge subsequently becomes transformed into an apical fold as the distal ectodermal epithelium grows and folds to enclose an extracellular space between the apposed basal surfaces of the epithelium. Collagen fibrils up to 2 micron in diameter, termed ‘actinotrichia’, are deposited along the proximo-distal axis in two (dorsal and ventral) arrays. The actinotrichia are aligned parallel to one another with a regular spacing along the greater part of their length. Mesenchymal cells migrating distally from the base of the fin bud encounter the dorsal and ventral arrays of actinotrichia and move between them apparently using the fibrils as a substratum. The entire structure is transparent and, using the killifish Aphyosemion scheeli, we have investigated the migration of the mesenchymal cells between 135 and 220 h of development, using Nomarski interference contrast microscopy and time-lapse video recording. The number of cellular processes per cell increased significantly during the period of observation. These processes could be graded according to their diameters. Processes of diameter greater than 2 micron were not usually aligned along actinotrichia and arose at any aspect of the cell body. In contrast, processes with diameters less than 2 micron appeared to be confined to the distal aspects of the migrating cells and showed an increasing tendency to become aligned as development progressed. Time-lapse video recordings revealed that such aligned processes move faster (mean speed 17.98 (+/− 2.25) micron/h) than non-aligned processes (mean speed 4.66 (+/− 0.67) micron/h). Whole cell translocation was generally slower than rates of process movement: the lowest mean value (1.52(+/− 0.36) micron/h) was recorded between 135 and 160 h of development rising to a maximum mean rate (4.72(+/− 0.42) micron/h) between 195 and 220 h; the period of the fastest rate of cell translocation correlated with maximum process alignment along actinotrichia. Thin 1 micron plastic sections revealed that, generally, aligned processes were in close association with the surface of the actinotrichial fibrils and not the spaces between them.

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