Attempts of Transmission of the Newt Melanoma by Nucleic Acids Preparations.

1974 ◽  
Vol 60 (2) ◽  
pp. 123-141
Author(s):  
Teresa Zavanella ◽  
Edgarda Camerini
Keyword(s):  
Biological Activity ◽  
Nucleic Acids ◽  
Geographical Origin ◽  
Triturus Cristatus ◽  
Normal Organ ◽  
Normal Tissues ◽  
Dnase Treatment ◽  
Cent Level ◽  
The Difference ◽  
Tumorigenic Activity

In the present study attempts were made to obtain DNA extracts with tumorigenic activity from the melanoma of the newt (Triturus cristatus Laur.). The DNA, prepared by two different methods from melanomas that had been transferred by cells for several generations, was injected subcutaneously in adult animals. Control animals were not treated or received DNA isolated from normal homologous organs. In all 320 animals were used, 238 of which were given DNA. The DNA preparations obtained according to the procedure of Colter et al. failed to induce tumors in any of the 111 inoculated animals, while the treatment with DNA extracted according to Kirby's method (127 cases) from tumors as well from normal tissues resulted in the development of melanomas with an incidence significantly higher than that observed in the group of untreated animals; however, the activity of the extracts was not modified by DNase treatment. The influence of factors such as neoplasia spread in the donors, duration of observation, geographical origin of animals and season of treatment was considered. Altogether 934 animals were studied, including those of other experimental series reported elsewhere: 670 were given nucleic acids extracted from tumors or normal organs, 204 received tumor or normal organ transplants, 60 were untreated. Of interest is the fact that the overall incidence of melanoma was much high in animals coming from areas of high industrialization, i.e. in the newts from the environs of Pavia (54.1 %), than in those from Naples (11 %): the difference between the two groups was highly significant and independent of the treatment. Due to the high tumor incidence in the newts from Pavia, no correlation could be demonstrated between biological activity of nucleic acid extracts and the above mentioned factors in the 670 animals treated. However, in the case of the newts from the Naples area a higher frequency of tumors was observed after treatment with nucleic acids extracted from melanoma, than after treatment with nucleic acids from normal organs: the result is statistically significant only at the 5 per cent level with the χ2 test.

Strategies for Oral Delivery of Metal-Saturated Lactoferrin

2019 ◽  
Vol 20 (11) ◽  
pp. 1046-1051 ◽  
Author(s):  
Przemysław Gajda-Morszewski ◽  
Klaudyna Śpiewak-Wojtyła ◽  
Maria Oszajca ◽  
Małgorzata Brindell
Keyword(s):  
Biological Activity ◽  
Oral Delivery ◽  
Therapeutic Potential ◽  
Structure And Function ◽  
The Difference ◽  
And Function ◽  
First Time

Lactoferrin was isolated and purified for the first time over 50-years ago. Since then, extensive studies on the structure and function of this protein have been performed and the research is still being continued. In this mini-review we focus on presenting recent scientific efforts towards the elucidation of the role and therapeutic potential of lactoferrin saturated with iron(III) or manganese(III) ions. The difference in biological activity of metal-saturated lactoferrin vs. the unmetalated one is emphasized. The strategies for oral delivery of lactoferrin, are also reviewed, with particular attention to the metalated protein.

Evaluation of Chemotherapeutic Activity of the Selected Bases’ Analogues of Nucleic Acids Supported by ab initio Various Quantum Chemical Calculations

2020 ◽  
Vol 16 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Piotr Kawczak ◽  
Leszek Bober ◽  
Tomasz Bączek
Keyword(s):  
Biological Activity ◽  
Nucleic Acids ◽  
Ab Initio ◽  
Quantum Chemical Calculations ◽  
Quantum Chemical ◽  
Structural Parameters ◽  
Principal Component ◽  
Polarizable Continuum Model ◽  
Activity Data ◽  
Qsar Models

Background: Pharmacological and physicochemical classification of bases’ selected analogues of nucleic acids is proposed in the study. Objective: Structural parameters received by the PCM (Polarizable Continuum Model) with several types of calculation methods for the structures in vacuo and in the aquatic environment together with the huge set of extra molecular descriptors obtained by the professional software and literature values of biological activity were used to search the relationships. Methods: Principal Component Analysis (PCA) together with Factor Analysis (FA) and Multiple Linear Regressions (MLR) as the types of the chemometric approach based on semi-empirical ab initio molecular modeling studies were performed. Results: The equations with statistically significant descriptors were proposed to demonstrate both the common and differentiating characteristics of the bases' analogues of nucleic acids based on the quantum chemical calculations and biological activity data. Conclusion: The obtained QSAR models can be used for predicting and explaining the activity of studied molecules.

scholarly journals Identification of novel DNA hypermethylation of the adenylate kinase 5 promoter in colorectal adenocarcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bokyung Ahn ◽  
Yang Seok Chae ◽  
Soo Kyung Lee ◽  
Moa Kim ◽  
Hyeon Soo Kim ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Lines ◽  
Adenylate Kinase ◽  
Dna Methyltransferase ◽  
Colorectal Adenocarcinoma ◽  
Adenine Nucleotides ◽  
Inverse Correlation ◽  
Dna Hypermethylation ◽  
Normal Tissues ◽  
The Difference

AbstractAdenylate kinase 5 (AK5) belongs to the adenylate kinase family that catalyses reversible phosphate transfer between adenine nucleotides, and it is related to various energetic signalling mechanisms. However, the role of AK5 in colorectal cancer (CRC) has not been reported. In this study, AK5 was significantly hypermethylated in CRC compared to adjacent normal tissues (P < 0.0001) and normal tissues (P = 0.0015). Although the difference in mRNA expression was not statistically significant in all of them, the selected 49 cases of CRC tissues with AK5 hypermethylation with the cut off value of 40% showed a significant inverse correlation with mRNA expression (P = 0.0003). DNA methylation of AK5 promoter significantly decreased and AK5 expression recovered by 5-aza-2′-deoxycytidine, DNA methyltransferase inhibitor in CRC cell lines. In addition, AK5 promoter activity significantly decreased due to DNA methyltransferase, and it increased due to 5-aza. Moreover, AK5 regulated the phosphorylated AMPK and mTOR phosphorylation and inhibited the cell migration and cell invasion in CRC cell lines. Furthermore, low AK5 expression is associated with poor differentiation (P = 0.014). These results demonstrate that the AK5 promoter is frequently hypermethylated and induced methylation-mediated gene down-regulation. AK5 expression regulates AMPK/mTOR signalling and may be closely related to metastasis in colorectal adenocarcinoma.

Biological Activity and Biotechnological Aspects of Locked Nucleic Acids

2013 ◽  
pp. 47-107 ◽  
Author(s):  
Karin E. Lundin ◽  
Torben Højland ◽  
Bo R. Hansen ◽  
Robert Persson ◽  
Jesper B. Bramsen ◽  
...  
Keyword(s):  
Biological Activity ◽  
Nucleic Acids ◽  
Locked Nucleic Acids

scholarly journals THE RELATIVE SIGNIFICANCE OF GRADED IMMUNIZING AND CHALLENGE DOSES IN MEASURING THE POTENCY OF VACCINES

1949 ◽  
Vol 90 (3) ◽  
pp. 233-253 ◽  
Author(s):  
H. C. Batson
Keyword(s):  
Response Curve ◽  
Standard Errors ◽  
Typhoid Vaccine ◽  
Relative Significance ◽  
Immunological Assay ◽  
Cent Level ◽  
The Difference ◽  
Graded Challenge ◽  
Level Of Significance ◽  
Dosage Response Curve

A study of the effect of graded immunizing doses of typhoid vaccine and graded challenge doses of S. typhosa in 5 per cent mucin on the degree of gradation of response (survival or death) elicited in successive groups of mice is reported. In the range of doses employed the effect of graded immunizing doses was markedly greater than was the effect of graded challenge doses. Statistically the difference exceeded the 0.1 per cent level of significance. It was concluded that the use of graded immunizing doses was preferable to the use of graded challenge doses as a basis for the immunological assay of typhoid vaccine, since with the former there was obtained (a) a greater significance of differences in response of groups given graded doses even with smaller fold increments in successive doses, (b) a greater slope of the dosage-response curve which permitted estimation of ED50 values with smaller standard errors, and (c) an effect on response apparently less dependent on the sex of mice used.

Influence of Botanical Species and Geographical Origin on the Content of Low Molecular Weight Phenolic Compounds of Woods Used in Portuguese Cooperage

Holzforschung ◽  
2000 ◽  
Vol 54 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Sara Canas ◽  
M. Conceição Leandro ◽  
M. Isabel Spranger ◽  
A. Pedro Belchior
Keyword(s):  
Molecular Weight ◽  
Phenolic Compounds ◽  
Geographical Origin ◽  
Castanea Sativa ◽  
Total Content ◽  
Low Molecular Weight ◽  
Qualitative And Quantitative ◽  
Botanical Species ◽  
The Difference ◽  
Chestnut Wood

Summary The influence of botanical species (Quercus and Castanea sativa) and geographical origin (Portugal—three different sites, France and North America) on the qualitative and quantitative content of some extractable low molecular weight phenolic compounds was assessed by HPLC. Chestnut wood had the highest total content of low molecular weight phenolic compounds, followed by the Portuguese oaks and the French oaks, whereas the American oak had the lowest content of these compounds. The contents of phenolic acids, phenolic aldehydes, scopoletin and umbelliferone were significantly different among the studied woods. Both the botanical species and the geographical origin affect the content of low molecular weight organic compounds of woods used in Portuguese cooperage. The results also show that the botanical species seem to be more important than the geographical origin to explain the difference in the wood phenolic composition. So, the separation of trees according to their origin is of considerable interest for coopers and winemakers to choose wisely the woods for the ageing of brandies.

Sign in / Sign up

Export Citation Format

Share Document