Algal Lymphadenitis in a Dog Caused by Scenedesmus Species

A 6-year-old, spayed female Labrador/Weimaraner cross-breed dog that had previously lived in Arizona presented in Montana for an annual examination with an incidentally enlarged popliteal lymph node, which was subsequently biopsied. Histologically, the lymph node was expanded by eosinophil-rich granulomas with both extracellular and intrahistiocytic green algae. These algae had intracytoplasmic, birefringent, and refractile granules; readily formed 2 to 3 mm green colonies on Columbia blood agar medium; and ultrastructurally had a multilayered cell wall and intracytoplasmic chloroplasts. Amplified product from the internal transcribed spacer and D1/D2 regions of the 28S ribosomal RNA gene had high sequence identity to Scenedesmus sp. Despite similar infection in the retropharyngeal lymph node 1 year later, the animal remained otherwise healthy with no clinical signs. To the authors’ knowledge, this is the first case of Scenedesmus species infection in a dog and is a differential diagnosis for Coccidioides immitis.

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Faculty Opinions recommendation of NMR structures of two designed proteins with high sequence identity but different fold and function.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1124240.581386 ◽

2008 ◽

Author(s):

H Jane Dyson ◽

Gira Bhabha

Keyword(s):

High Sequence Identity ◽

Sequence Identity ◽

Nmr Structures ◽

Designed Proteins ◽

And Function

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Efficacy of Intravenous Liposomal Amphotericin B (AmBisome) against Coccidioidal Meningitis in Rabbits

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.46.8.2420-2426.2002 ◽

2002 ◽

Vol 46 (8) ◽

pp. 2420-2426 ◽

Cited By ~ 65

Author(s):

Karl V. Clemons ◽

Raymond A. Sobel ◽

Paul L. Williams ◽

Demosthenes Pappagianis ◽

David A. Stevens

Keyword(s):

Spinal Cord ◽

Amphotericin B ◽

Liposomal Amphotericin ◽

White Blood Cells ◽

Clinical Signs ◽

Liposomal Amphotericin B ◽

Coccidioides Immitis ◽

Lower Protein ◽

Motor Abnormalities ◽

The Brain

ABSTRACT The efficacy of intravenously administered liposomal amphotericin B (AmBisome [AmBi]) for the treatment of experimental coccidioidal meningitis was compared with those of oral fluconazole (FLC) and intravenously administered conventional amphotericin B (AMB). Male New Zealand White rabbits were infected by intracisternal inoculation of arthroconidia of Coccidioides immitis. Starting 5 days postinfection, animals received one of the following: 5% dextrose water diluent; AMB given at 1 mg/kg of body weight; AmBi given at 7.5, 15, or 22.5 mg/kg intravenously three times per week for 3 weeks; or oral FLC given at 80 mg/kg for 19 days. One week after the cessation of therapy, all survivors were euthanatized, the numbers of CFU remaining in the spinal cord and brain were determined, and histological analyses were performed. All AmBi-, FLC-, or AMB-treated animals survived and had prolonged lengths of survival compared with those for the controls (P < 0.0001). Treated groups had significantly lower numbers of white blood cells and significantly lower protein concentrations in the cerebrospinal fluid compared with those for the controls (P < 0.01 to 0.0005) and had fewer clinical signs of infection (e.g., weight loss, elevated temperature, and neurological abnormalities including motor abnormalities). The mean histological scores for AmBi-treated rabbits were lower than those for FLC-treated and control rabbits (P < 0.016 and 0.0005, respectively); the scores for AMB-treated animals were lower than those for the controls (P < 0.0005) but were similar to those for FLC-treated rabbits. All regimens reduced the numbers of CFU in the brain and spinal cord compared with those for the controls (P ≤0.0005). AmBi-treated animals had 3- to 11-fold lower numbers of CFU than FLC-treated rabbits and 6- to 35-fold lower numbers of CFU than AmB-treated rabbits. Three of eight animals given 15 mg of AmBi per kg had no detectable infection in either tissue, whereas other doses of AmBi or FLC cleared either the brain or the spinal cord of infection in fewer rabbits. In addition, clearance of the infection from both tissues was achieved in none of the rabbits, and neither tissue was cleared of infection in AMB-treated animals. Overall, these data indicate that intravenously administered AmBi is superior to oral FLC or intravenous AMB and that FLC is better than AMB against experimental coccidioidal meningitis. These data indicate that AmBi may offer an improvement in the treatment of coccidioidal meningitis. Additional studies are warranted.

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First Case of Autochthonous Equine Theileriosis in Austria

Pathogens ◽

10.3390/pathogens10030298 ◽

2021 ◽

Vol 10 (3) ◽

pp. 298

Author(s):

Esther Dirks ◽

Phebe de Heus ◽

Anja Joachim ◽

Jessika-M. V. Cavalleri ◽

Ilse Schwendenwein ◽

...

Keyword(s):

Developmental Stages ◽

Elevated Serum ◽

Clinical Signs ◽

Dermacentor Reticulatus ◽

Theileria Equi ◽

Amyloid A ◽

First Case ◽

Blood Smears ◽

Eastern Austria ◽

Iatrogenic Transmission

A 23-year-old pregnant warmblood mare from Güssing, Eastern Austria, presented with apathy, anemia, fever, tachycardia and tachypnoea, and a severely elevated serum amyloid A concentration. The horse had a poor body condition and showed thoracic and pericardial effusions, and later dependent edema and icteric mucous membranes. Blood smear and molecular analyses revealed an infection with Theileria equi. Upon treatment with imidocarb diproprionate, the mare improved clinically, parasites were undetectable in blood smears, and 19 days after hospitalization the horse was discharged from hospital. However, 89 days after first hospitalization, the mare again presented to the hospital with an abortion, and the spleen of the aborted fetus was also PCR-positive for T. equi. On the pasture, where the horse had grazed, different developmental stages of Dermacentor reticulatus ticks were collected and subjected to PCR, and one engorged specimen was positive for T. equi. All three amplicon sequences were identical (T. equi genotype E). It is suspected that T. equi may repeatedly be transmitted in the area where the infected mare had grazed, and it could be shown that transmission to the fetus had occurred. Due to the chronic nature of equine theileriosis and the possible health implications of infection, it is advised to include this disease in the panel of differential diagnoses in horses with relevant clinical signs, including horses without travel disease, and to be aware of iatrogenic transmission from inapparent carrier animals.

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Right inferior phrenic artery pseudoaneurysm after a laparoscopic gastrectomy: a case report

Surgical Case Reports ◽

10.1186/s40792-019-0739-x ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Kaoruko Funakoshi ◽

Yuji Ishibashi ◽

Shuntaro Yoshimura ◽

Ryoto Yamazaki ◽

Fumihiko Hatao ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Dissection ◽

Laparoscopic Gastrectomy ◽

Rare Complication ◽

Clinical Signs ◽

Node Dissection ◽

Artery Pseudoaneurysm ◽

Inferior Phrenic Artery ◽

Thermal Spread ◽

Phrenic Artery

Abstract Background Ruptured pseudoaneurysms are a rare complication of gastrectomy, but when they do develop, they are often fatal. We presented herein the first report of a case of pseudoaneurysm arising from the right inferior phrenic artery (RIPA) after a laparoscopic gastrectomy. Case presentation A 61-year-old male patient underwent a laparoscopic distal gastrectomy and D1+ lymph node dissection with Roux-en-Y reconstruction for early gastric cancer. He was discharged on postoperative day (POD) 9 without any complications, such as anastomotic or pancreatic leakage. On POD 19, he was referred to the emergency room for upper abdominal pain. Enhanced abdominal computed tomography revealed a 60 × 70 mm hematoma, indicating intra-abdominal bleeding and a 10-mm pseudoaneurysm in the RIPA. Selective digital subtraction angiography confirmed the presence of a pseudoaneurysm in the RIPA, which was embolized using multiple microcoils. Thereafter, no clinical signs were observed, and the patient was discharged from the hospital 15 days after angiography without any recurrence of bleeding. We hypothesized that the cause of the pseudoaneurysm was mechanical vascular injury due to the thermal spread of the ultrasonically activated devices (USADs) during lymphatic node dissection. Conclusion Given the thermal spread of USADs, safe and appropriate lymph node dissection based on precise anatomical knowledge is crucial to preventing postoperative pseudoaneurysms.

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Acute upper airway obstruction as a life-threatening complication of ventral bulla osteotomy: report of two consecutive cases

Journal of Feline Medicine and Surgery Open Reports ◽

10.1177/20551169211005923 ◽

2021 ◽

Vol 7 (1) ◽

pp. 205511692110059

Author(s):

Michal Vlasin ◽

Richard Artingstall ◽

Barbora Mala

Keyword(s):

Airway Obstruction ◽

Clinical Signs ◽

Upper Airway ◽

Upper Airway Obstruction ◽

Single Session ◽

Inflammatory Polyp ◽

Case Summary ◽

First Case ◽

Life Threatening

Case summary This paper presents two cases of acute postoperative upper airway obstruction following ventral bulla osteotomy (VBO) in cats. The first cat underwent a unilateral left-sided VBO for a suspected inflammatory polyp. The second cat underwent a single-session bilateral VBO procedure for bilateral otitis media. In the first case, immediate re-intubation and a gradual lightening of the anaesthetic plane resolved the clinical signs; in the second case, the patient deteriorated and went into acute cardiorespiratory arrest and received cardiopulmonary resuscitation. Both patients recovered well and were discharged home 3 days after surgery. Both cases were reported to show no further clinical signs on postoperative follow-up 3 weeks and 4 months after surgery, respectively. Relevance and novel information Upper airway obstruction should be regarded as a potential complication of VBO in cats.

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PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.2.499-502.2002 ◽

2002 ◽

Vol 9 (2) ◽

pp. 499-502 ◽

Cited By ~ 3

Author(s):

Lynn M. Herrmann ◽

Timothy V. Baszler ◽

Donald P. Knowles ◽

William P. Cheevers

Keyword(s):

Lymph Node ◽

Peripheral Blood ◽

Infected Sheep ◽

Peripheral Blood Leukocytes ◽

Retropharyngeal Lymph Node ◽

Blood Leukocytes

ABSTRACT Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% ± 0.28% of 3 × 106 DRLN cells, but were not detected in 3 × 106 PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 × 106 PBLs.

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Effectiveness of superselective intra-arterial chemoradiotherapy targeting retropharyngeal lymph node metastasis

European Archives of Oto-Rhino-Laryngology ◽

10.1007/s00405-016-3933-5 ◽

2016 ◽

Vol 273 (10) ◽

pp. 3331-3336 ◽

Cited By ~ 3

Author(s):

Takayoshi Suzuki ◽

Tomohiro Sakashita ◽

Akihiro Homma ◽

Hiromitsu Hatakeyama ◽

Satoshi Kano ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Retropharyngeal Lymph Node ◽

Node Metastasis

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Transoral Excision of a Retropharyngeal Lymph Node under Rigid Endoscopic Control

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348941011900401 ◽

2010 ◽

Vol 119 (4) ◽

pp. 211-214 ◽

Cited By ~ 7

Author(s):

Laura Buttà ◽

Davide Lombardi ◽

Anna Marconi ◽

Piero Nicolai

Keyword(s):

Lymph Node ◽

Retropharyngeal Lymph Node ◽

Endoscopic Control

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Portland Cement Use in Dental Root Perforations: A Long Term Followup

Case Reports in Dentistry ◽

10.1155/2014/637693 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5

Author(s):

Álvaro Henrique Borges ◽

Matheus Coelho Bandeca ◽

Mateus Rodrigues Tonetto ◽

Luis Augusto Faitaroni ◽

Elibel Reginna de Siqueira Carvalho ◽

...

Keyword(s):

Portland Cement ◽

Calcium Hydroxide ◽

Root Canal ◽

Clinical Signs ◽

Signs And Symptoms ◽

Clinical Aspects ◽

Iatrogenic Perforation ◽

Root Canals ◽

First Case ◽

Clinical Signs And Symptoms

Root canal and furcal perforations are causes of endodontic therapy failure and different materials that stimulate tissue mineralization have been proposed for perforation treatment. In the first case, a patient presented tooth 46 with unsatisfactory endodontic treatment and a periapical radiographic lesion. A radiolucent area compatible with a perforating internal resorption cavity was found in the mesial root. The granulation tissue was removed, and root canals were prepared. The intracanal medication was composed of calcium hydroxide and the perforation cavity was filled with Portland cement. The 11-year followup showed radiographic repair of the tissue adjacent to the perforation and absence of clinical signs and symptoms or periapical lesion. In the second case, a patient presented with edema on the buccal surface of tooth 46. The examination showed a radiolucent area in the furcation region compatible with an iatrogenic perforation cavity. The mesial root canals were calcified, and only the distal root canal was prepared. The cavity was filled with a calcium hydroxide-based paste and the distal root canal was obturated. In sequence, the perforation cavity was filled with Portland cement. The 9-year followup showed the tooth in masticatory function with radiographic and clinical aspects compatible with normality.

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