A Neoplasm of Globule Leukocytes in a Cat

1986 ◽  
Vol 23 (3) ◽  
pp. 287-292 ◽  
Author(s):  
D. J. Honor ◽  
D. B. De Nicola ◽  
J. J. Turek ◽  
J. A. Render ◽  
D. A. Serra
Keyword(s):  
Lymph Nodes ◽  
Electron Density ◽  
Single Unit ◽  
Mononuclear Cells ◽  
Phosphotungstic Acid ◽  
Periodic Acid ◽  
Domestic Cat ◽  
Unit Membrane ◽  
Periodic Acid Schiff ◽  
Tracheobronchial Lymph Nodes

A neoplasm involving the ileo-cecal-colic junction, thymus, and tracheobronchial lymph nodes of a 7-year-old domestic cat was composed of dense sheets of round to oval mononuclear cells with oval to indented nuclei, moderate amounts of cytoplasm, and variable numbers of round eosinophilic granules. These granules are brown to black in phosphotungstic acid hematoxylin-stained sections and stain variably with the periodic acid-Schiff stain. They are 0.8 to 1.5 μm in diameter, limited by a single unit membrane, and have variable electron density. Light microscopic cellular morphology and staining characteristics as well as ultrastructural features of these cells are consistent with feline globule leukocytes. Morphologic features of the neoplastic cells in the present case are similar to those of the only other reported neoplasm of globule leukocytes which also involved the intestine of a cat.

Immunohistochemical Demonstration of Keratin in Canine Neuroepithelioma

1987 ◽  
Vol 24 (6) ◽  
pp. 500-503 ◽  
Author(s):  
W. Baumgärtner ◽  
P. V. Peixoto
Keyword(s):  
Spinal Cord ◽  
Glial Fibrillary Acidic Protein ◽  
Fourth Ventricle ◽  
Phosphotungstic Acid ◽  
Squamous Metaplasia ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Periodic Acid Schiff ◽  
The Third ◽  
Intradural Extramedullary

Morphological features and immunoreactivity for cytokeratin (CK), glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) of three canine neuroepitheliomas and three canine ependymomas were investigated. Neuroepitheliomas were in three German shepherds as intradural-extramedullary solitary masses, with spinal cord displacement between T10 and L2. Histologically, they contained tubules and acini, lined by epithelial cells with focal squamous metaplasia, rosette-like structures, and polygonal to spindle-shaped cells between tubules. Acini were empty or filled with a homogeneous, eosinophilic periodic acid-Schiff (PAS)-positive material. Mitotic indices varied from low to moderate. Ependymomas occurred in the third (two cases) and fourth ventricle in adult boxers. Histologically, they were composed of cells with an ill-defined, scant amphophilic cytoplasm, with a central round euchromatic nucleus; cells formed pseudorosettes, with a central fibro-vascular stroma. Neuroepitheliomas stained for CK, but ependymomas did not. Both failed to stain for GFAP, NSE, or phosphotungstic acid hematoxylin (PTAH). Thus, antibodies to cytokeratin are useful to distinguish neuroepitheliomas from ependymomas.

scholarly journals Case Reports: Disseminated Chlorellosis in a Dog

2009 ◽  
Vol 46 (3) ◽  
pp. 439-443 ◽  
Author(s):  
R. R. Quigley ◽  
K. E. Knowles ◽  
G. C. Johnson
Keyword(s):  
Lymph Nodes ◽  
Case Reports ◽  
Periodic Acid ◽  
Electron Microscopic Examination ◽  
Mesenteric Lymph Nodes ◽  
Electron Microscopic ◽  
Periodic Acid Schiff ◽  
First Report ◽  
Dense Bodies ◽  
Mesenteric Lymph

An adult dog with ataxia and a lingual mass, previously diagnosed as protothecosis, was euthanized. At the postmortem examination, the lingual mass, regions of the lungs and hilar lymph nodes, liver, mesenteric and sublumbar lymph nodes, and spinal meninges had pronounced green discoloration. Histologically, pyogranulomatous inflammation and algal organisms were found in the tongue, spinal meninges, hilar and mesenteric lymph nodes, liver, and lung. The algae had cell walls positive for periodic acid-Schiff and cytoplasmic granules. Ultrastructurally, the algae had a well-defined cell wall, stacks of grana and thylakoid membrane, and dense bodies, typical of starch granules. The organisms were identified as Chlorella, a green alga, based on the results of histochemistical and electron microscopic examination. To the author's knowledge this is the first report of disseminated Chlorella infection and the first report in a companion animal.

Globoid Cell-like Leukodystrophy in a Domestic Longhaired Cat

2002 ◽  
Vol 39 (4) ◽  
pp. 494-496 ◽  
Author(s):  
C. J. Sigurdson ◽  
R. J. Basaraba ◽  
E. M. Mazzaferro ◽  
D. H. Gould
Keyword(s):  
Mononuclear Cells ◽  
Periodic Acid ◽  
Clinical Signs ◽  
Krabbe Disease ◽  
Periodic Acid Schiff ◽  
Nervous Systems ◽  
Central Nervous Systems ◽  
Mental Regression ◽  
Globoid Cell ◽  
Globoid Cells

Globoid cell leukodystrophy (GLD; Krabbe disease), is a rare heritable metabolic disorder in humans, dogs, mutant twitcher mice, and rhesus monkeys that is caused by a deficiency in the lysosomal enzyme galactocerebrosidase (GALC). GALC deficiency results in the accumulation of psychosine, which is toxic to oligodendrocytes and Schwann cells of the central and peripheral nervous systems. Clinical signs include hypotonia, mental regression, and death by 2 years of age in most human patients. Here we describe a domestic longhaired kitten with rapidly progressive neurologic disease and brain and spinal cord lesions characteristic of GLD. Pathologic hallmarks of the disease reflect the loss of oligodendrocytes and include myelin loss, gliosis, and the perivascular accumulation of large mononuclear cells with fine cytoplasmic vacuoles (globoid cells) in the peripheral and central nervous systems. Globoid cells were CD68 and ferritin positive, confirming their monocytic origin, and cytoplasmic contents were nonmetachromatic and periodic acid-Schiff positive.

Immunohistochemical and Ultrastructural Features of Hepatocellular Cytoplasmic Globules in Venous Outflow Impairment

2019 ◽  
Vol 152 (5) ◽  
pp. 563-569
Author(s):  
Alessia Buglioni ◽  
Tsung-Teh Wu ◽  
Taofic Mounajjed
Keyword(s):  
Electron Microscopy ◽  
Random Distribution ◽  
Phosphotungstic Acid ◽  
Periodic Acid ◽  
Complement Protein ◽  
Budd Chiari Syndrome ◽  
Venous Outflow ◽  
Periodic Acid Schiff ◽  
Chiari Syndrome ◽  
Size Variability

Abstract Objectives To examine the immunohistochemical and ultrastructural features of hepatocellular cytoplasmic globules in venous outflow impairment (VOI). Methods Sixty-four liver core biopsies were screened. Patients with α-1 antitrypsin (AAT) deficiency were excluded. All biopsies were stained with H&E, Masson trichrome, periodic acid-Schiff with diastase digestion (PAS-D), phosphotungstic acid hematoxylin (PTAH), complement protein 4d (C4d) immunostain, and AAT immunostain. Electron microscopy was also performed. Results Hepatocellular globules were identified in 8% of in-house cases. Causes of VOI included heart failure and Budd-Chiari syndrome. The hepatocellular cytoplasmic globules showed size variability, random distribution, and positivity for PAS-D, PTAH, and AAT. C4d was inconsistently positive. Electron microscopy showed that the globules were lysosome-bound inclusions containing microfibrillar material and fibrinogen. Conclusions PAS-D–positive hepatocellular globules occur in VOI. They cross-react with AAT but have different appearance, localization, and ultrastructural composition from globules in AAT deficiency.

scholarly journals STRUCTURE AND STAINING CHARACTERISTICS OF MYOFIBER EXTERNAL LAMINA

1973 ◽  
Vol 21 (8) ◽  
pp. 703-714 ◽  
Author(s):  
S. I. ZACKS ◽  
M. F. SHEFF ◽  
A. SAITO
Keyword(s):  
Cell Membrane ◽  
Phosphotungstic Acid ◽  
Divalent Cations ◽  
Periodic Acid ◽  
Amorphous Layer ◽  
Ruthenium Red ◽  
Low Ph ◽  
Acid Solutions ◽  
Colloidal Iron ◽  
Periodic Acid Schiff

A variety of cytochemical methods were used to investigate the composition of mouse sarcolemma. We found that the sarcolemma is a complex composed of the myofiber cell membrane, a thin zone external to the cell membrane that contains groups that bind colloidal iron and thorium at low pH and a thicker amorphous layer that fails to stain with colloidal iron and thorium at low pH. The entire complex is periodic acid-Schiff-positive and stains with ruthenium red and strongly acid solutions of phosphotungstic acid. Although the specificity of these cytochemical stains is controversial, data obtained with them and from preliminary analyses of myofiber external lamina (EL) indicate that the myofiber cell coat is chiefly composed of glycoprotein containing a large number of carboxyl groups. The EL within the subneural apparatus of the neuromuscular junction differs from noninnervated areas in the fusion of Schwann cell and myofiber EL, the absence of collagen microfibrils, the more intense binding of divalent cations and the less intense stain with phosphotungstic acid in strongly acid solutions.

scholarly journals Adenocarcinoma of Apocrine Sweat Glands in a Mouflon (Ovis Musimon)

2005 ◽  
Vol 17 (4) ◽  
pp. 389-392 ◽  
Author(s):  
Federico Morandi ◽  
Cinzia Benazzi ◽  
Paolo Simoni
Keyword(s):  
Epithelial Cells ◽  
Lymph Nodes ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Sweat Glands ◽  
Left Shoulder ◽  
Free Living ◽  
Periodic Acid Schiff ◽  
Ovis Musimon ◽  
Ultrastructural Findings

A free-living mouflon ( Ovis musimon) was presented with a mass on the left shoulder. At necropsy, multifocal, slightly protruding whitish spots were noted on the kidneys, and several lymph nodes were abnormal. Histologically, the mass was composed of epithelial cells arranged in tubular and tubulopapillary structures. The cytoplasm of the epithelial cells had numerous periodic acid–Schiff-positive and diastase-resistant granules. Ultrastructurally, the cytoplasm of the neoplastic cells contained numerous pleomorphic secretory granules and microvilli, which partially covered the luminal surface of the tumor cells. Metastatic foci were present in pres-capular and mediastinal lymph nodes and kidneys. On the basis of histological and ultrastructural findings, this tumor was diagnosed as a tubulopapillary adenocarcinoma, arising from apocrine sweat glands of the skin.

scholarly journals Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244160
Author(s):  
Guang-ping Ruan ◽  
Xiang Yao ◽  
Qing-keng Lin ◽  
Zi-an Li ◽  
Xue-min Cai ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Mononuclear Cells ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Periodic Acid ◽  
Renal Ischemia ◽  
Model Group ◽  
Periodic Acid Schiff ◽  
Peripheral Blood Mononuclear ◽  
Renal Ischemia Reperfusion Injury

Ischemia-reperfusion injury is an important contributor to acute kidney injury and a major factor affecting early functional recovery after kidney transplantation. We conducted this experiment to investigate the protective effect of induced multipotent stem cell transplantation on renal ischemia-reperfusion injury. Forty rabbits were divided into four groups of 10 rabbits each. Thirty rabbits were used to establish the renal ischemia-reperfusion injury model, and ten rabbits served as the model group and were not treated. Among the 30 rabbits with renal ischemia-reperfusion injury, 10 rabbits were treated with induced peripheral blood mononuclear cells (PBMCs), and 10 other rabbits were treated with noninduced PBMCs. After three weekly treatments, the serum creatinine levels, urea nitrogen levels and urine protein concentrations were quantified. The kidneys were stained with hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Masson’s trichrome and then sent for commercial metabolomic testing. The kidneys of the rabbits in the model group showed different degrees of pathological changes, and the recovery of renal function was observed in the group treated with induced cells. The results indicate that PBMCs differentiate into multipotent stem cells after induction and exert a therapeutic effect on renal ischemia-reperfusion injury.

Proteinaceous Lymphadenopathy in a Young Patient With History of Classical Hodgkin Lymphoma: A Case Report With Literature Review

2018 ◽  
Vol 27 (2) ◽  
pp. 176-180 ◽  
Author(s):  
Oleksandr Kravtsov ◽  
Ronald Jaffe ◽  
Gabriela Gheorghe
Keyword(s):  
Case Report ◽  
Literature Review ◽  
Lymph Nodes ◽  
Young Patient ◽  
Hodgkin Lymphoma ◽  
Periodic Acid ◽  
Classical Hodgkin Lymphoma ◽  
Periodic Acid Schiff ◽  
History Of ◽  
Uncertain Etiology

Proteinaceous lymphadenopathy (PLD) is a poorly defined, underreported pathological entity of uncertain etiology characterized by massive deposition of amorphous, eosinophilic, and periodic acid-Schiff–positive material involving lymph nodes, which is distinct from amyloid and clonal immunoglobulin deposition. PLD can resemble collagen sclerosis and needs to be differentiated from lymphomas with sclerosis, particularly classical Hodgkin lymphoma, nodular sclerosis type, and therefore is an important pitfall in the diagnosis of lymphoma with sclerosis. We are reporting a young patient with history of classical Hodgkin lymphoma who eventually developed PLD and review the literature on this subject.

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

1993 ◽  
Vol 30 (3) ◽  
pp. 256-264 ◽  
Author(s):  
D. W. Hayden ◽  
D. J. Waters ◽  
B. A. Burke ◽  
J. C. Manivel
Keyword(s):  
Lymph Nodes ◽  
Tumor Cells ◽  
Mononuclear Cells ◽  
Lectin Binding ◽  
Periodic Acid ◽  
Golden Retriever ◽  
Malignant Histiocytosis ◽  
Regional Lymph Nodes ◽  
Human T Cell ◽  
The Right

Diagnosis of malignant histiocytosis (MH), a disorder characterized by systemic proliferation of morphologically atypical histiocytes and their precursors, in an 8-year-old neutered female Golden Retriever was based on light and electron microscopic and immunohistochemical findings. Clinically, the dog presented with unilateral forelimb lameness. Eight days after surgical exploration of a swollen brachium, the dog developed sudden onset of posterior paresis, fecal and urinary incontinence, and a flaccid tail. Necropsy revealed infiltrative and nodular lesions in the right forelimb and regional lymph nodes, thoracic and abdominal cavities, and lumbar epidural space. Gross lesions were not found in the lungs or integument. Histopathologic examination showed infiltrates of atypical histiocytes in skeletal muscle, joint, and regional lymph nodes of the right forelimb; intercostal muscle; lung; liver; spleen; pancreas; kidneys; and spinal dura. Most tumor infiltrates were nodular and composed of loosely aggregated cells that were 10-30 μm in diameter with abundant eosinophilic to foamy cytoplasm, had central or eccentric nuclei, and were periodic acid-Schiff negative. Many binucleated cells, multinucleated giant cells, and mitotic figures were seen. Tumor cells contained phagocytosed erythrocytes, mononuclear cells, and some leukocytes. Ultrastructural features of tumor cells included cytoplasmic lipid droplets, lysosomes, and phagolysosomes. Immunohistochemical studies on paraffin-embedded sections showed positive reactivity to human T-cell Ag (clone UCHL-1) and for lysozyme, α-1-antitrypsin, and cathespin B. Polyclonal intracellular immunoglobulin reactivity and lectin binding (peanut, soybean, and wheat germ agglutinins and concanavalin A) were also demonstrated. Criteria for diagnosis of malignant histiocytic tumors and differential diagnosis are discussed.

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