scholarly journals Erratum

Cephalalgia ◽  
2018 ◽  
Vol 38 (7) ◽  
pp. NP1-NP1
Keyword(s):  
Animal Model ◽  
Electrical Stimulation ◽  
Single Dose ◽  
Sodium Pentobarbital ◽  
Sprague Dawley ◽  
Print Version ◽  
C Fibers ◽  
Sprague Dawley Rats ◽  
Number Of Cells ◽  
Stimulation Of

Zhao Y, Martins-Oliveira M, Akerman S, and Goadsby PJ. Comparative effects of traditional Chinese and Western migraine medicines in an animal model of nociceptive trigeminovascular activation. Cephalalgia. Epub ahead of print 24 August 2017. DOI: 10.1177/0333102417728245 In this article, some data was incorrectly reported in the following sentences. The corrections are shown in bold font below: Page 2: Fifty-four male Sprague-Dawley rats (250–370 g) were anesthetized using a single dose of sodium pentobarbital (60 mg kg−1 i.p.; Nembutal, Diamondback Drugs, Scottsdale, AZ) for induction, and propofol (20–25 mg kg−1 h−1 i.v., Propoflo, Abbott, Abbott Park, IL, USA) for maintenance throughout the experiment. Page 3: The data collected as post-stimulus histograms after electrical stimulation of the dura mater for Ad-fibers represent the number of cells fired over at least a 10 ms period in the region 5–20 ms, and for C-fibers 20–80 ms, post-stimulation over the 20 collections. Page 3: Recordings were made from 54 neurons (in 54 rats) responsive to dural stimulation. The print version of this article has been corrected.

Endogenous CCK disrupts the MMC pattern via capsaicin-sensitive vagal afferent fibers in the rat

1997 ◽  
Vol 272 (1) ◽  
pp. G100-G105 ◽  
Author(s):  
A. Rodriguez-Membrilla ◽  
P. Vergara
Keyword(s):  
Intravenous Infusion ◽  
Sprague Dawley ◽  
Rat Intestine ◽  
Intracerebroventricular Infusion ◽  
C Fibers ◽  
Afferent Fibers ◽  
Sprague Dawley Rats ◽  
Vagal Afferent ◽  
Endogenous Release ◽  
Stimulation Of

A meal disrupts migrating motor complexes (MMC) in the rat intestine through stimulation of peripheral cholecystokinin (CCK)-B and central CCK-A receptors. The aim of this study was to determine pathways implicated in postprandial disruption of the MMC mediated by CCK. Sprague-Dawley rats were prepared with electrodes for electromyography in the small intestine, and ablation of vagal afferent C-fibers by capsaicin was carried out. Endogenous release of CCK was induced by oral administration of soybean trypsin inhibitor (SBTI). In control rats SBTI disrupted MMC and generated an irregular spiking activity that lasted longer than 3 h. Intravenous infusion of L-365,260 (2 x 10(-7) mol/kg) but not of L-364,718 (3 x 10(-9) mol/kg) restored the MMC pattern. In capsaicin-treated rats, SBTI did not modify fasting activity. Infusion of CCK octapeptide (CCK-8) at 3 x 10(-9) mol.kg-1.h-1 disrupted the MMC, although the response was quantitatively and qualitatively different from SBTI. The effect was reversed by intravenous infusion of L-364,718 or L-365,260 and intracerebroventricular infusion of L-364,718. In capsaicin-treated rats, the intracerebroventricular or intravenous infusion of L-364,718 inhibited CCK-8 effects. However, the intravenous infusion of L-365,260 did not reverse the MMC pattern. These results suggest that the disruption of the MMC mediated by CCK is due to stimulation of peripheral CCK-B receptors located in vagal afferent fibers. This initiates a reflex including stimulation of central CCK-A receptors. Exogenous CCK also stimulates peripheral CCK-A receptors not located in capsaicin-sensitive vagal afferent fibers.

TRPV1 receptor blockade is ineffective in different in vivo models of migraine

Cephalalgia ◽  
2010 ◽  
Vol 31 (2) ◽  
pp. 172-180 ◽  
Author(s):  
Oliver Summ ◽  
Philip R Holland ◽  
Simon Akerman ◽  
Peter J Goadsby
Keyword(s):  
Electrical Stimulation ◽  
Sensory Processing ◽  
Dynamic Range ◽  
Sprague Dawley ◽  
In Vivo Models ◽  
Trpv1 Receptor ◽  
C Fibers ◽  
Trpv1 Receptors ◽  
Stimulation Of

Background: It has been proposed that TRPV1 receptors may play a role modulating trigeminal sensory processing. We used models of trigeminovascular nociceptive activation to study the involvement of TRPV1 receptors in the rat. Due to a possible role of TRPV1 receptors in cortical spreading depression (CSD), an experimental phenomenon sharing many features with migraine aura, we also utilized a model of mechanically induced CSD. Methods: Male Sprague Dawley rats ( N = 39) were anesthetized and cannulated for monitoring and drug administration to study the effects of the TRPV1 receptor antagonist A-993610 (8 mg kg−1 IV). Wide-dynamic-range neurons, responding to electrical stimulation of the middle meningeal artery (MMA)/dura mater were identified and recorded using electrophysiological techniques. Intravital microscopy was used to study neurogenic dural vasodilation (NDV) of the MMA comparing capsaicin and electrical stimulation, and the effect of A-993610 on mechanically induced CSD was examined. Results: Administration of A-993610 had no significant effect on trigeminal firing of A- or C-fibers elicited by electrical stimulation of the MMA. It also showed no effect on NDV whilst blocking vasodilation due to intravenous capsaicin injection. The mechanically induced CSD response could not be altered by A-993610 administration. Conclusions: Although there is evidence that TRPV1 receptors play an important role in sensory processing in general, the new data do not support a role in the treatment of acute migraine.

Effects of 2, 5-Dimethoxy-4-Methylamphetamine (DOM, STP) on Successive Sensory Discrimination Behavior Maintained by Electrical Stimulation of the Brain Reinforcement in the Rat

1976 ◽  
Vol 38 (3_suppl) ◽  
pp. 1083-1092 ◽  
Author(s):  
John H. Vincent ◽  
Irmingard I. Lenzer
Keyword(s):  
Electrical Stimulation ◽  
Discrimination Task ◽  
Lever Press ◽  
Behavioral Effects ◽  
Sprague Dawley ◽  
Response Latencies ◽  
Sensory Discrimination ◽  
Sprague Dawley Rats ◽  
The Brain ◽  
Stimulation Of

The effects of DOM (2, 5-dimethoxy-4-methylamphetamine) on behavior reinforced by electrical stimulation of the brain were observed in five male Sprague-Dawley rats. The animals were trained on a successive discrimination task: the SD interval lasted as long as it took the animal to make one lever-press; the SΔ interval was variable, with a mean duration of 60 sec. Following DOM administration, response latencies to the SD were longer during the first 75 min. and SΔ responding was augmented over the 2-hr. session. Gross behavioral effects such as hypokinesia and ataxia were observed for a large part of the session. While increased response latencies may be attributed to hypokinesia and ataxia, increases in SΔ responding reflect a breakdown of discrimination itself. Severe behavioral depression was not observed, suggesting that electrical stimulation of the brain may have counteracted the depressive effect of the amphetamine.

scholarly journals Evaluation of the Single-Dose Toxicity of Capsaicin Pharmacopuncture in Rats

2020 ◽  
Vol 37 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Ji Hye Hwang ◽  
Jaseung Ku ◽  
Chul Jung
Keyword(s):  
Animal Model ◽  
Single Dose ◽  
Saline Solution ◽  
Sprague Dawley ◽  
Control Groups ◽  
Male And Female ◽  
Sprague Dawley Rats ◽  
Body Weights ◽  
And Control ◽  
Safe Dose

Background: This study aimed to assess the toxicity of capsaicin (CP) pharmacopunture in an animal model.<br/>Methods: The toxicity of a single-muscular dose of CP (45.45 mg/mL) was evaluated in 6-week-old male and female Sprague-Dawley rats. A total of 20 rats were assigned to 2 groups which were sex and weight matched. All rats acclimatized for 1 week before receiving 1.0 mL of CP (45.45 mg/mL) or normal saline solution(control) intramuscularly. The general condition and mortality of the animals were observed. The rats were sacrificed 2 weeks after CP was administered and histopathology was performed.<br/>Results: No abnormal symptoms or deaths were observed, and there was no difference in body weights between the CP and control groups throughout the study. No significant differences in histopathology were observed between the groups.<br/>Conclusion: No toxicological changes related to the administration of CP were observed. This study indicated that the safe dose of CP in Sprague-Dawley rats was 1.0 mL of CP (45.45 mg/mL) or less. Further studies are needed to confirm the safety of CP in the human body.

Effects of Sodium Phenobarbital on Brain Stimulation Behavior, Behavioral Seizures, and EEG Seizure Activity

1978 ◽  
Vol 42 (3) ◽  
pp. 1007-1016 ◽  
Author(s):  
Sharon N. Schnare ◽  
Irmingard I. Lenzer
Keyword(s):  
Electrical Stimulation ◽  
Brain Stimulation ◽  
Seizure Activity ◽  
Sprague Dawley ◽  
Continuous Reinforcement ◽  
Sprague Dawley Rats ◽  
Sodium Phenobarbital ◽  
The Brain ◽  
Rate Of Response ◽  
Stimulation Of

The effects of sodium phenobarbital on (a) behavior reinforced by electrical stimulation of the brain, (b) behavioral seizures, and (c) EEG seizure activity were observed in seven male Sprague-Dawley rats. Rate of response on placebo day, over a 30-min. continuous reinforcement session, was compared to rate of response on drug day; an increase in response on the drug day over the placebo day was called a positive phenobarbital effect and a decrease a negative phenobarbital effect. For some animals the positive phenobarbital effect disappeared when the animal's rate of response was calculated for seizure-free time, i.e., when the time spent in seizure was subtracted from the 30-min. period. For other animals, however, the phenobarbital effect, whether positive or negative, was not directly related to time gained on the drug day compared to the placebo day. A new concept was advanced, that of seizure-proneness, measured by the number and duration of seizures and spike after-discharges. Significant correlations were found for seizure-proneness and phenobarbital effect.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

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