The effect of bovine serum albumin-glutaraldehyde and polyethylene glycol polymer on neointimal hyperplasia in rabbit carotid artery anastomosis

2020 ◽  
pp. 088532822096491
Author(s):  
Erturk Karaagac ◽  
Yuksel Besir ◽  
Meltem Kurus ◽  
Orhan Gokalp ◽  
Sahin Iscan ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Carotid Artery ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Neointimal Hyperplasia ◽  
The Other ◽  
Control Group ◽  
Positive Staining ◽  
Staining Score

Objective Since the systemic drugs have been used to reduce the hyperplasic response in the tunica intima, the periadventitial local drug applications to the vascular wall have gained more popularity. In this study, we investigated the effect of bovine serum albumin-glutaraldehyde and polyethylene glycol polymer on neointimal hyperplasia in rabbit carotid artery anastomosis to explore the effects of these two different agents. Methods 21 New Zealand male rabbits were randomly divided into three groups. The carotid artery transection and anastomosis was performed onthe control group. The bovine serum albumin-glutaraldehyde and the polyethylene glycol polymer were applied locally on the other two groups seperatley after transection and anastomosis of the carotid arteries. At the end of 28-day follow-up, the histological and the immunohistochemical results related to neointimal hyperplasia were compared. Results The glue residues were detected in the BSA-glutaraldehyde group, but in the PEG polymer group there was no glue residue. The intima thickness and the intima/media thickness ratio in the control group was significantly higher (p<0.05) than the other groups. These values did not differ significantly between the BSA-glutaraldehyde group and the PEG polymer group (p>0.05). The lumen diameter and the area in the control group were significantly higher (p < 0.05) than the BSA-glutaraldehyde group. These values between the control group and the PEG polymer group did not differ significantly (p>0.05). aSMA-positive staining score in the Control group was found to be significantly lower (p < 0.05) than the BSA-glutaraldehyde and PEG polymer group and the VEGF-positive staining score in the control group was found to be significantly higher (p < 0.05) than the BSA-glutaraldehyde and the PEG polymer group. Conclusions Although the both agents have positive results on neointimal hyperplasia, it would be favorable to use polyethylene glycol polymer, since it does not seem to affect the lumen area and the lumen diameter of the vessel.

scholarly journals Direct evidence for the involvement of domain III in the N-F transition of bovine serum albumin

1986 ◽  
Vol 236 (1) ◽  
pp. 307-310 ◽  
Author(s):  
M Y Khan
Keyword(s):  
Bovine Serum Albumin ◽  
Acid Solution ◽  
Serum Albumin ◽  
Protein Sequence ◽  
Bovine Serum ◽  
Direct Evidence ◽  
Structural Transformations ◽  
The Other ◽  
Ph Range ◽  
Domain Iii

The domain III of bovine serum albumin containing residues 377-582 of the protein sequence was isolated and its behaviour in acid solution was studied. The fragment was found to undergo structural transformations over the pH range 3.5-4.5 known to cause N-F transition in serum albumin. On the other hand, an albumin fragment that was devoid of domain III was unable to exhibit such a transition. These results were consistent with a mechanism where N-F transition involves the separation of domain III from the rest of the albumin starts at about pH 4.3 and is completed at pH 3.5.

Human corticotropin (ACTH) radioimmunoassay with synthetic 1--24 ACTH.

1979 ◽  
Vol 25 (7) ◽  
pp. 1267-1273 ◽  
Author(s):  
P C Kao ◽  
N S Jiang ◽  
P C Carpenter
Keyword(s):  
Polyethylene Glycol ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Confidence Limit ◽  
Normal Subjects ◽  
Cross Reactivity ◽  
Normal Range ◽  
Beta Endorphin

Abstract A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody-bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.

Interaction Mechanism between Bovine Serum Albumin and Polyethylene Glycol Revealed with Elastic Light Scattering Spectroscopy

2013 ◽  
Vol 704 ◽  
pp. 43-50
Author(s):  
Wen Zhi Zhang ◽  
Kuan Zhang ◽  
Jian Long Zheng ◽  
Hong Shu Chen ◽  
Wei Xing Chen ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Light Scattering ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Interaction Mechanism ◽  
Interaction Force ◽  
Elastic Light Scattering ◽  
Apparent Binding Constant ◽  
Light Scattering Spectroscopy

In order to gain deeper insight into the interaction mechanism between bovine serum albumin (BSA) and polyethylene glycol (PEG), the present work applied elastic light scattering (ELS) spectroscopy to investigate the interaction between BSA and PEG, and explore the effects of concentration and molecular weight of PEG on the interaction at physiological pH. The results showed that the interaction force existed between linear PEG and spherical BSA molecules was mainly hydrogen bonding. In addition, the apparent binding constant of system was evaluated by model calculation.

scholarly journals Experimental Study on MICP Aqueous Solution under the Action of Different Organic Substrates

Geofluids ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Huai-miao Zheng ◽  
Ling-ling Wu ◽  
Kai-wen Tong ◽  
Lin Hu ◽  
Qing Yu ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Mass Fraction ◽  
Organic Matrix ◽  
Egg White ◽  
Control Group ◽  
Organic Substrates ◽  
Carbonate Formation

The precipitation rate and cementation strength of calcium carbonate crystals during the process of microorganism-induced calcium carbonate precipitation (MICP) are key factors that affect the application effect of this technology. In order to improve the quality of calcium carbonate formation in the MICP process, egg white protein with a volume fraction of 20%, bovine serum albumin with a mass fraction of 0.3%, sucrose with a mass fraction of 5%, bamboo leaves with a mass concentration of 25 g/L, and bamboo leaf-magnesium chloride (Mg2+/Ca2+=4:1) were added during the experiment of different groups of MICP solutions. The results of the solution test study showed that there was no obvious lag period for bacterial growth under the action of organic matrix. The concentration of bacteria in the reaction solution was higher under the action of sucrose and egg white. The conversion rate of Ca2+ under the action of egg white was the fastest, which was about 2.5 times higher than that of the control group. After 14 days of grouting reaction, it was found that the proportion of calcite-type calcium carbonate produced under the action of egg white was the highest, and the Ca element accounted for 66.24% in the solidified material. Sucrose was second; bovine serum albumin was the lowest. The calcium carbonate crystals generated by the control of each organic matrix had a high degree of pore size matching with the tailings under a dry-wet cycle. The structural characteristics of the calcium carbonate crystals, such as crystal form, morphology, and particle size, were mainly due to the interaction between the organic matrix and the calcium carbonate crystals. This study proves that the addition of the organic matrix can improve the formation rate and crystal structure of calcium carbonate during MICP, thus providing a new reference for the development of MICP technology.

RADIOIMMUNOASSAY OF THYROTROPHIN RELEASING HORMONE (TRH)

1974 ◽  
Vol 77 (3) ◽  
pp. 417-421 ◽  
Author(s):  
T. J. Visser ◽  
R. Docter ◽  
G. Hennemann
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
The Other ◽  
Thyrotrophin Releasing Hormone ◽  
Releasing Hormone ◽  
Double Antibody

ABSTRACT Immunization of rabbits with a conjugate in which L-pGlu-L-His-L-Pro-NH2 (TRH) at the amide side has been coupled to bovine serum albumin (BSA) yielded anti-TRH antibody which was detected by reaction of diluted antiserum with 3H-TRH. A double antibody radioimmunoassay has been developed in which 100 pg unlabelled TRH could be detected. Several analogues tested showed that only pGlu-His-Pro-OMe possessed comparable reactivity, while all the other compounds exhibited very little activity in the assay.

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