Prevalence of Epstein-Barr Virus and Human Papillomavirus in Cervical Samples from Women Attending an STD-Clinic

A group of 91 women attending the STD-clinic, Department of Dermato-venereology, Sahlgrenska Hospital, Gothenburg, were screened for EBV DNA and HPV DNA of the cervix with the PCR-technique. Presence of EBV DNA was demonstrated in 35 (38%) women and HPV DNA in 30 (33%) women. Fourteen (15%) women had both EBV DNA and HPV DNA present. Without the colposcope 20 of these women had macroscopic signs of HPV infection on the vulva and/or vagina and 71 had no signs of infection. Presence of EBV DNA was not correlated to clinical signs of HPV infection.

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Detection of Human Papillomavirus Genotypes and Epstein-Barr Virus in Nasopharyngeal Carcinomas at the Korle-Bu Teaching Hospital, Ghana

The Scientific World JOURNAL ◽

10.1155/2017/2721367 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Du-Bois Asante ◽

Richard Harry Asmah ◽

Andrew Anthony Adjei ◽

Foster Kyei ◽

David Larbi Simpong ◽

...

Keyword(s):

Human Papillomavirus ◽

Epstein Barr Virus ◽

Far East ◽

Pcr Analysis ◽

Hpv Dna ◽

Barr Virus ◽

Hpv Genotypes ◽

Formalin Fixed Paraffin Embedded ◽

Ebv Dna ◽

Epstein Barr

Nasopharyngeal carcinomas (NPC) are endemic in Far East Asia and commonly harbour Epstein-Barr virus (EBV) which is known to serve as a key oncogenic promoter. Human papillomavirus (HPV) is known to contribute to the pathogenesis of NPC. However, in Ghana these two viruses have not been linked to NPC prevalence. This study was designed to determine the HPV genotypes and EBV involved in NPC tissue biopsies. A retrospective study design involving 72 formalin-fixed paraffin-embedded tissue (FFPET) samples of NPC from 2006 to 2012 were retrieved from the Department of Pathology, University of Ghana School of Biomedical and Allied Health Sciences. Sections were taken for histological analysis and for DNA lysate preparation. The DNA lysates were subjected to polymerase chain reaction (PCR) analysis to determine the presence of HPV genotypes and EBV. HPV specific primers were used to type for fourteen HPV genotypes (HPV-16, 18, 6/11, 31, 33, 35, 44, 42, 43, 45, 56, 52, 58, and 59). Out of the 72 NPC biopsies analyzed by PCR, EBV DNA was present in 18 (25%) cases and HPV DNA in 14 (19.23%). High risk HPV (HR-HPV) genotypes 18 and 31 were associated with the NPC. There were 3 (4.2%) cases of coinfection by both viruses. The EBV DNA present in the undifferentiated variant of the NPC and the histopathology of the NPC in Ghana is similar to the type described in endemic areas.

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Demonstration of Epstein-Barr Virus DNA in Acetowhite Lesions of the Vulva

International Journal of STD & AIDS ◽

10.1177/095646249400500107 ◽

1994 ◽

Vol 5 (1) ◽

pp. 25-28 ◽

Cited By ~ 7

Author(s):

E Voog ◽

A Ricksten ◽

G B Löwhagen ◽

A Ternesten

Keyword(s):

Human Papilloma Virus ◽

Epstein Barr Virus ◽

Control Group ◽

Papilloma Virus ◽

Hpv Dna ◽

Barr Virus ◽

Oral Hairy Leukoplakia ◽

Hairy Leukoplakia ◽

Ebv Dna ◽

Epstein Barr

Acetowhite lesions in the vulva disclosing koilocytosis have been related to infection by human papilloma virus (HPV). Because of the clinical resemblance of these lesions to oral hairy leukoplakia (OHL), and EBV-manifestation, 23 women with acetowhite koilocytotic lesions in the vulva were examined. The PCR-technique was used to detect EBV DNA as well as HPV DNA in 17% of 23 patients examined. In a control group of 19 patients EBV DNA was detected in 11% and HPV DNA in 42% of cell samples from normal vulvar mucosa. EBV DNA has not previously been demonstrated in the vulvar mucosa, and this virus might be related to subclinical acetowhite lesions.

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Human Papillomavirus-Associated Lymphoepithelioma-Like Carcinoma of the Anal Canal: A Case Report and Literature Review

Frontiers in Medicine ◽

10.3389/fmed.2021.766960 ◽

2021 ◽

Vol 8 ◽

Author(s):

Weiwei Weng ◽

Weiqi Sheng ◽

Lei Wang

Keyword(s):

Human Papillomavirus ◽

Anal Canal ◽

Epstein Barr Virus ◽

Viral Infections ◽

Hpv Infection ◽

Rare Type ◽

Barr Virus ◽

T Cell Infiltration ◽

Epstein Barr ◽

High Risk Hpv

Lymphoepithelioma-like carcinoma is a rare type of tumor that is histologically identical to lymphoepithelial carcinoma of the nasopharynx. Lymphoepithelioma-like carcinomas (LELCs) are closely associated with viral infections. Human papillomavirus (HPV)-associated LELCs have been reported in a variety of anatomic sites. We reported an extremely rare case of a 25-year-old woman with LELC derived from the anal canal, which is the second case reported at this site. The tumor was diffusely positive for p16 staining, and was correlated with high-risk HPV-16; Epstein-Barr virus-encoded small RNA was negative; PD-L1 positivity and abundant CD8+ T cell infiltration were observed, indicating a “hot” immune microenvironment. In reporting this case, we highlight the potential for misdiagnosis and suggested an association of HPV infection with LELC in the anal canal.

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Are acetowhite lesions of the cervix correlated to the presence of Epstein-Barr virus DNA?

International Journal of STD & AIDS ◽

10.1258/0956462971920488 ◽

1997 ◽

Vol 8 (7) ◽

pp. 432-436 ◽

Cited By ~ 3

Author(s):

Ewa Voog ◽

Anne Ricksten ◽

Monica Stenglein ◽

Fredrik Jonassen ◽

Annika Ternesten ◽

...

Keyword(s):

In Situ Hybridization ◽

Predictive Factor ◽

Epstein Barr Virus ◽

Control Group ◽

Study Group ◽

Hpv Dna ◽

Barr Virus ◽

Ebv Dna ◽

Epstein Barr

The purpose of this study was to investigate whether Epstein-Barr virus (EBV) is associated with acetowhite lesions of the portio cervix, demonstrating koilocytosis and/or cervical intraepithelial neoplasia (CIN) I-III. The study group comprised 37 women admitted to the Department of Gynaecology and Obstetrics, Sahlgrenska University Hospital, Go teborg because of pathological colposcopy or cytology of the portio cervix. Colposcopically, all exhibited acetowhite lesions of the portio cervix. Cells were sampled with a cytobrush for examination for EBV and human papillomavirus (HPV) DNA by polymerase chain reaction (PCR) and a biopsy was taken for histopathology. Biopsies from 5 patients positive for EBV by PCR in cervical cell samples were examined by an in situ hybridization technique for EBER (Epstein-Barr virus encoded RNA), RNAs expressed in latent EBV infection. The control group consisted of women attending the Department of Dermato-Venereology at the same hospital for STD check-up. These had a normal cytology and no signs of acetowhiteness of the portio cervix. In the study group, EBV DNA was found in 30% and HPV DNA in 51%. In the control group 57% exhibited EBV DNA and 23% HPV DNA. EBV was not found to be a predictive factor in the development of koilocytosis and/or CIN I-III. HPV was a predictive factor in acetowhite, koilocytotic lesions. No expression of EBER was found in the 5 biopsies examined by in situ hybridization.

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Epstein-Barr virus (EBV) genes expression in cervical intraepithelial neoplasia and invasive cervical cancer: A comparative study with human papillomavirus (HPV) infection

Human Pathology ◽

10.1016/s0046-8177(00)80245-2 ◽

2000 ◽

Vol 31 (3) ◽

pp. 318-326 ◽

Cited By ~ 38

Author(s):

T SASAGAWA ◽

M SHIMAKAGE ◽

M NAKAMURA ◽

J SAKAIKE ◽

H ISHIKAWA ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Comparative Study ◽

Epstein Barr Virus ◽

Invasive Cervical Cancer ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Barr Virus ◽

Genes Expression ◽

Epstein Barr

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Mapping Human Papillomavirus, Epstein-Barr Virus, Cytomegalovirus, Adenovirus, And P16 In Laryngeal Cancer

10.21203/rs.3.rs-1225790/v1 ◽

2022 ◽

Author(s):

Alexandra Schindele ◽

Anna Holm ◽

Karin Nylander ◽

Annika Allard ◽

Katarina Olofsson

Keyword(s):

High Risk ◽

Laryngeal Cancer ◽

Epstein Barr Virus ◽

Viral Infections ◽

Surrogate Marker ◽

Hpv Infection ◽

Oncogenic Viruses ◽

Hpv Dna ◽

Barr Virus ◽

Epstein Barr

Abstract Purpose: Apart from tobacco and alcohol, viral infections are proposed as risk factors for laryngeal cancer. The occurrence of oncogenic viruses including human papilloma virus (HPV) and Epstein-Barr virus (EBV), in laryngeal squamous cell carcinoma (LSCC) varies in the world. Carcinogenesis is a multi-step process, and the role of viruses in LSCC progression has not been clarified. We aimed to analyze the presence and co-expression of HPV, EBV, human cytomegalovirus (HCMV) and human adenovirus (HAdV) in LSCC. We also investigated if p16 can act as surrogate marker for HPV in LSCC. Methods: Combined PCR/microarrays (PapilloCheck®) were used for detection and genotyping of HPV DNA, real time-PCR for EBV, HCMV and HAdV DNA detection, and EBER in situ hybridization (EBER-ISH) for EBV detection in tissue from 78 LSCC patients. Additionally, we analyzed p16 expression with immunohistochemistry.Results: Thirty-three percent (26/78) of LSCC tumor samples were EBV positive, 9% (7/78) HCMV positive and 4% (3/78) HAdV positive. Due to DNA fragmentation, 45 samples could not be analyzed with PapilloCheck®; 9% of the remaining (3/33) were high-risk HPV16 positive and also over-expressed p16. A total of 14% (11/78) of the samples over-expressed p16.Conclusion: These findings present a mapping of HPV, EBV, HCMV and HAdV, including the HPV surrogate marker p16, in LSCC in this cohort. Except for EBV, which was detected in a third of the samples, data show viral infection to be uncommon, and that p16 does not appear to be a specific surrogate marker for high-risk HPV infection in LSCC.

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Evaluation of the Prevalence of Human Papillomavirus and Epstein-Barr Virus in Esophageal Squamous Cell Carcinomas

The International Journal of Biological Markers ◽

10.1177/172460080502000102 ◽

2005 ◽

Vol 20 (1) ◽

pp. 5-10 ◽

Cited By ~ 10

Author(s):

I.D. Lyronis ◽

S. Baritaki ◽

I. Bizakis ◽

M. Tsardi ◽

D.A. Spandidos

Keyword(s):

Human Papillomavirus ◽

Squamous Cell ◽

Epstein Barr Virus ◽

Hpv Infection ◽

Greek Population ◽

Hpv 16 ◽

Barr Virus ◽

Hpv Status ◽

Epstein Barr ◽

Hpv 18

The aim of this study was to determine the possible involvement of human papillomavirus and Epstein-Barr virus in esophageal squamous cell cancer (ESCC) carcinogenesis in the Greek population. DNA was extracted from 30 ESCC and 27 normal esophageal specimens and screened for HPV type-specific or EBV infection by PCR-based assay. Seventeen out of 30 ESCC specimens (56%) were found positive for HPV DNA, of which 15 (88%) were typed as HPV-18 infected, one (5.9%) as HPV-16 infected, and one (5.9%) as infected by an HPV type different from the studied HPV-6, 11, 16, 18 and 33 subtypes. Six of the 27 normal esophageal specimens (22.2%) were positive for HPV infection, five typed as HPV-18 (83.3%) and one as HPV-16 (16.7%). All samples were negative for EBV genome detection as assessed by the PCR assay. No statistically significant correlation was found between the HPV status of the tumor samples and clinical parameters including sex, age of the patients, tobacco or alcohol use, differentiation grade of the lesions and stage of the disease. In conclusion, our findings indicate a statistically significant (p<0.001) overall association between ESCC and HPV infection, mostly related to the HPV-18 subtype, in the Greek population.

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Role of Epstein-Barr Virus and Human Papillomavirus Coinfection in Cervical Intraepithelial Neoplasia in Chinese Women Living With HIV

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.703259 ◽

2021 ◽

Vol 11 ◽

Author(s):

Min Feng ◽

Rufei Duan ◽

Yang Gao ◽

Han Zhang ◽

Youlin Qiao ◽

...

Keyword(s):

Gene Expression ◽

Cervical Cancer ◽

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Epstein Barr Virus ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Barr Virus ◽

Epstein Barr

Given that only a small percentage of human papillomavirus (HPV)-positive women develop cancer, HPV is necessary but insufficient for carcinogenesis. Mucosally transmitted viral cofactors appear to contribute to HPV-related cervical cancer, such as Epstein-Barr virus (EBV), but previous studies have shown inconsistent outcomes. The exact role of EBV in cervical cancer remains unclear, and more studies are needed to determine its involvement. In this study, we describe the prevalence of EBV and HPV coinfection in HIV-positive women and explore how abnormal host immune status induced by viral coinfections modulates epithelial gene expression. We found a significant correlation between EBV-HPV coinfection and the incidence of high-grade cervical intraepithelial neoplasia (CIN2+). RNA sequencing indicated that CIN tissues coinfected with EBV and HPV led to significant changes in the gene expression of epithelial differentiation and development compared to normal tissues with HPV infection alone. In particular, several differentially expressed genes (DEGs) are closely associated with cancer, such as CACNG4, which was confirmed to be upregulated at both the mRNA and protein levels. Therefore, these findings provide some evidence that EBV may act as a cofactor or mediator in HPV-related cervical cancer. Specific genes or proteins, such as CACNG4, may serve as biomarkers that can risk stratify patients based on pathological changes in the cervix.

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