Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride

2010 ◽  
Vol 29 (5) ◽  
pp. 409-418 ◽  
Author(s):  
E. Kasi Viswanadh ◽  
B. Nageshwar Rao ◽  
BS Satish Rao
Keyword(s):  
Cadmium Chloride ◽  
Mangifera Indica ◽  
Optimal Dose ◽  
Protective Role ◽  
Reduction Factor ◽  
Post Treatment ◽  
Glutathione S Transferase ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Antigenotoxic Effect

Cadmium is an environmental metal toxin implicated in human diseases. Mangiferin (MGN), a naturally occurring glucosylxanthone, is present in Mangifera indica. In this study, the protective role of MGN against cadmium chloride (CdCl2)-induced genotoxicity was studied in Swiss albino mice. Mice were administered with single intra-peritoneal (i.p.) optimal dose of MGN (2.5 mg/kg b.wt.) before treatment with various concentrations of CdCl2 (7, 8, 9, 10 and 11 mg/kg b.wt.). The LD 50(30) was found to be 8.5 mg/kg b.wt. for DDW + CdCl2 group, while it was increased to 9.77 mg/kg after MGN treatment resulting in increase in the LD50(30) value by 1.26 mg, with a dose reduction factor (DRF) of 1.14. Treatment of mice to various doses of CdCl2 resulted in a dose-dependent increase in the frequency of micronucleated polychromatic (MnPCE) and normochromatic erythrocytes (MnNCE), with corresponding decrease in the polychromatic / normochromatic erythrocyte ratio (PCE/NCE ratio) at various post-treatment times. MGN (2.5 mg/kg b.wt.) pretreatment significantly (p < .001) reduced the frequency of MnPCE, MnNCE and increased PCE/NCE ratio when compared with the DDW + CdCl2 group at all post-treatment times indicating its antigenotoxic effect. Further, pretreatment of MGN declined the lipid peroxidation (LPx) content in liver, whereas significant increase was observed in hepatic Glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity. Our study revealed that MGN has potent antigenotoxic effect against CdCl2-induced toxicity in mice, which may be due to the scavenging of free radicals and increased antioxidant status.

scholarly journals Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alperen Gündüz ◽  
Emine Yalçın ◽  
Kültiğin Çavuşoğlu
Keyword(s):  
Tap Water ◽  
Radical Scavenging ◽  
Treated Group ◽  
Protective Role ◽  
Toxic Effects ◽  
Control Group ◽  
Buccal Epithelium ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Cytogenetic Parameters

AbstractIn this study, the toxic effects of aflatoxin B2 (AFB2) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, creatinine, malondialdehyde-MDA and glutathione-GSH) and cytogenetic parameters (micronucleus-MN in buccal epithelium, erythrocyte and leukocyte cells and chromosomal aberrations-CAs) were used to determine the toxic effects. Additionally, scavenging effects of resveratrol against superoxide, hydrogen peroxide (H2O2) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were also investigated. In experimental period, mice were divided into six groups and the groups were treated with tap water, 10 mg/kg b.w resveratrol, 20 mg/kg b.w resveratrol, 20 µg/kg b.w. AFB2, 10 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, 20 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, respectively. As a result, the scavenging effects of resveratrol increased with increasing dose and the superoxide, H2O2 and DPPH radical scavenging activity of resveratrol were 74.9%, 79.1% and 49.2%, respectively. AFB2 administration caused a significant decrease in physiological parameters, and these decreases regressed in AFB2 + resveratrol treated groups. Serum ALT and AST activities, BUN and creatinine levels were higher in the AFB2 treated group compared to the control group and serious abnormalities were found in MDA and GSH levels in the kidney and liver. In the group treated with AFB2 + 20 mg/kg resveratrol, ALT, AST, BUN and creatinine levels decreased significantly and GSH levels increased compared to only-AFB2 treated group. AFB2 triggered MN formation in buccal epithelium, erythrocyte and leukocyte cells and CAs in bone marrow cells. The application of 20 mg/kg resveratrol together with AFB2 was decreased the MN and CAs frequency. Resveratrol exhibited a recovery effect in the range of 40.9–80.5% against AFB2 toxicity in all tested parameters. In this study, it was determined that AFB2 caused serious changes in selected physiological, biochemical and cytogenetic parameters while resveratrol displayed a protective role against these toxic effects.

scholarly journals Antidiarrhoeal efficacy of Mangifera indica seed kernel on Swiss albino mice

2012 ◽  
Vol 5 (8) ◽  
pp. 630-633 ◽  
Author(s):  
S Rajan ◽  
H Suganya ◽  
T Thirunalasundari ◽  
S Jeeva
Keyword(s):  
Mangifera Indica ◽  
Seed Kernel ◽  
Swiss Albino Mice ◽  
Albino Mice

Dose-dependent suppression of haloperidol-induced catalepsy by potentized Agaricus muscarius

1996 ◽  
Vol 85 (03) ◽  
pp. 141-144 ◽  
Author(s):  
Souvik Ghosh ◽  
S.P. Sinha Babu ◽  
N.C. Sukul
Keyword(s):  
Treatment Effect ◽  
Post Treatment ◽  
Active Principle ◽  
Mechanical Agitation ◽  
Swiss Albino Mice ◽  
Before And After ◽  
Albino Mice ◽  
Pre Treatment ◽  
Dose Dependent ◽  
Post Treatment Effect

Abstract Agaricus muscarius 30c, a potentized homoeopathic drug prepared by successive dilution with 90% alcohol followed by sonication, suppressed haloperidol-induced catalepsy in Swiss albino mice significantly. This anticataleptic effect was dose-dependent being greatest with the undiluted Agaricus 30c and least if diluted 1:20,000. Higher dilutions like 1:40,000 and 1:50,000 did not produce an anticataleptic effect. The effect reappeared when the 1:50,000 dilution was sonicated. The anticataleptic effect of potentized Agaricus was observed with the drug administered both before and after haloperidol. However, the pre-treatment effect was more pronounced than the post-treatment effect.It is thought that potentized Agaricus contains an active principle that can be attenuated by dilution and multiplied by mechanical agitation or sonication.

Protective role of Solanum trilobatum (Solanaeace) against benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice

2014 ◽  
Vol 4 (4) ◽  
pp. 535-541 ◽  
Author(s):  
R. Venugopal ◽  
V. Mahesh ◽  
G. Ekambaram ◽  
A. Aadithya ◽  
D. Sakthisekaran
Keyword(s):  
Protective Role ◽  
Lung Carcinogenesis ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Solanum Trilobatum

Reversal of Cadmium Chloride-Induced Oxidative Stress and Genotoxicity by Adhatoda vasica Extract in Swiss Albino Mice

2006 ◽  
Vol 111 (1-3) ◽  
pp. 217-228 ◽  
Author(s):  
Tamanna Jahangir ◽  
Tajdar Husain Khan ◽  
Lakshmi Prasad ◽  
Sarwat Sultana
Keyword(s):  
Oxidative Stress ◽  
Cadmium Chloride ◽  
Adhatoda Vasica ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals Protective Effect ofAdhatoda vasciaNees against Radiation-Induced Damage at Cellular, Biochemical and Chromosomal Levels in Swiss Albino Mice

2007 ◽  
Vol 4 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Meenal Kumar ◽  
Ravindra Samarth ◽  
Madhu Kumar ◽  
Senthamil R. Selvan ◽  
Begraj Saharan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Ethanolic Extract ◽  
Reduction Factor ◽  
Swiss Albino Mice ◽  
Unani Medicine ◽  
Radiation Induced ◽  
Albino Mice ◽  
Induced Changes ◽  
Marrow Cells

Extract ofAdhatoda vasica(L) Nees leaves has been used for treatment of various diseases and disorders in Ayurved and Unani medicine. Modulatory effect of ethanolic extract ofA. vasica(L) Nees against radiation-induced changes in terms of histological alterations in testis, reduced glutathione (GSH), lipid peroxidation (LPO), acid and alkaline phosphatases levels, and chromosomal alterations in Swiss albino mice was studied at various post-irradiation intervals between 1 and 30 days. Mice exposed to 8 Gy radiation showed radiation-induced sickness including marked changes in histology of testis and chromosomal aberrations in bone marrow cells with 100% mortality within 22 days. When ethanolic leaf extract ofA. vasicawas given orally at a dose of 800 mg kg−1body weight per mouse for 15 consecutive days and then exposed to radiation, death ofAdhatoda-pretreated irradiated mice was reduced to 70% at 30 days. The radiation dose reduction factor was 1.43. There was significantly lesser degree of damage to testis tissue architecture and various cell populations including spermatogonia, spermatids and Leydig cells. Correspondingly, a significant decrease in the LPO and an increase in the GSH levels were observed in testis and liver ofAdhatoda-pretreated irradiated mice. Similarly, a significant decrease in level of acid phosphatase and increase in level of alkaline phosphatase were observed.Adhatodapretreatment significantly prevented radiation-induced chromosomal damage in bone marrow cells. The study suggests thatAdhatodaplant extract has significant radioprotective effects on testis that warrants further mechanistic studies aimed at identifying the role of major ingredients in the extract.

Protective role of epigallocatechin-3-gallate on arsenic induced testicular toxicity in Swiss albino mice

2017 ◽  
Vol 96 ◽  
pp. 685-694 ◽  
Author(s):  
Pushpa Rani Guvvala ◽  
Janivara Parameswaraiah Ravindra ◽  
Chukkath Vijayan Rajani ◽  
Muniandy Sivaram ◽  
Sellappan Selvaraju
Keyword(s):  
Protective Role ◽  
Testicular Toxicity ◽  
Swiss Albino Mice ◽  
Albino Mice
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