The transcription factor FOXA1 induces epithelial ovarian cancer tumorigenesis and progression

FOXA1 (forkhead box A1), a member of the FOXA transcription factor superfamily, plays an important role in tumor occurrence and development. However, the relationship between FOXA1 and ovarian cancer has not been reported. We examined normal ovarian tissue and ovarian cancer tissue and found increased FOXA1 expression in the cancer tissue. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays demonstrated that transfection with small interfering RNA to silence FOXA1 (si-FOXA1) in ovarian cancer cell lines decreased cell proliferation and induced apoptosis and S-phase arrest. In addition, si-FOXA1 transfection inhibited cell migration and invasion. Western blotting showed that si-FOXA1 transfection decreased the levels of YY1-associated protein 1, cyclin-dependent kinase 1, cyclin D1, phosphatidylinositol-3 kinase, E2F transcription factor 1, B-cell lymphoma 2, and vascular endothelial growth factor A protein. Based on these results, we suggest that FOXA1 plays a catalytic role in ovarian cancer pathogenesis and development by affecting the expression of the above-mentioned proteins.

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Long Noncoding RNA LINC00858 Promotes the Progression of Ovarian Cancer via Regulating the miR-134-5p/TRIM44 Axis

10.21203/rs.3.rs-30879/v1 ◽

2020 ◽

Author(s):

Ning Wang ◽

Qin-Xue Cao ◽

Jun Tian ◽

Lu Ren ◽

Hai-Ling Cheng ◽

...

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Migration And Invasion ◽

Ovarian Cancer Cells ◽

Cancer Tissue ◽

Loss Of Function ◽

Ovarian Cancer Tissue ◽

Skov3 Cells ◽

Tissue Specimens

Abstract Background Ovarian cancer remains one of the most challenging areas of cancer research. Recent studies have shown that many long non-coding RNAs (lncRNAs) are abnormally expressed in ovarian cancer and involved in the pathological progress of ovarian cancer. In the present study, we aimed to investigate the role of lncRNA LINC00858 and the potential mechanism in ovarian cancer. Methods The qRT-PCR was used to measure the expression levels of LINC00858 and miR-134-5p in ovarian cancer tissue specimens and cell lines. Loss-of-function assays were performed to investigate the role of LINC00858 in ovarian cancer progression. MTT assay was carried out to measure cell proliferation. Transwell assays were performed to determine the migration and invasion of ovarian cancer cells. Biological information analysis and luciferase report gene assay were used to verify potential downstream genes of LINC00858. The xenograft mouse model was established to analyze tumor growth in vivo. Results Our results showed that LINC00858 was highly expressed in human ovarian cancer tissue specimens and cell lines. Loss-of-function assays showed that knockdown of LINC00858 significantly inhibited cell proliferation, migration and invasion of SKOV3 cells, and suppressed tumor growth in mouse xenograft models. Mechanistic studies revealed that LINC00858 acted as a sponge of miR-134-5p and then regulated the expression TRIM44 in SKOV3 cells. Furthermore, rescue experiments illustrated that inhibition of miR-134-5p restored the inhibitory effects of LINC00858 knockdown on ovarian cancer cell proliferation, migration and invasion. TRIM44 overexpression could counteract the inhibitory effects of miR-134-5p mimics on ovarian cancer cells. Conclusion In conclusion, these findings demonstrated that LINC00858 exerted oncogenic role in ovarian cancer, which was mediated by miR-134-5p/TRIM44 axis. Thus, LINC00858 might be a therapeutic target for the treatment of ovarian cancer.

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Identification of Novel Biomarkers and Candidate Drug in Ovarian Cancer

Journal of Personalized Medicine ◽

10.3390/jpm11040316 ◽

2021 ◽

Vol 11 (4) ◽

pp. 316

Author(s):

Chia-Jung Li ◽

Li-Te Lin ◽

Pei-Yi Chu ◽

An-Jen Chiang ◽

Hsiao-Wen Tsai ◽

...

Keyword(s):

Ovarian Cancer ◽

Ovarian Tissue ◽

High Expression ◽

Cancer Tissue ◽

Ovarian Cancer Tissue ◽

Kaplan Meier ◽

Candidate Drug ◽

Therapeutic Value ◽

Novel Biomarkers ◽

Low Expression

This paper investigates the expression of the CREB1 gene in ovarian cancer (OV) by deeply excavating the gene information in the multiple databases and the mechanism thereof. In short, we found that the expression of the CREB1 gene in ovarian cancer tissue was significantly higher than that of normal ovarian tissue. Kaplan–Meier survival analysis showed that the overall survival was significantly shorter in patients with high expression of the CREB1 gene than those in patients with low expression of the CREB1 gene, and the prognosis of patients with low expression of the CREB1 gene was better. The CREB1 gene may play a role in the occurrence and development of ovarian cancer by regulating the process of protein. Based on differentially expressed genes, 20 small-molecule drugs that potentially target CREB1 with abnormal expression in OV were obtained from the CMap database. Among these compounds, we found that naloxone has the greatest therapeutic value for OV. The high expression of the CREB1 gene may be an indicator of poor prognosis in ovarian cancer patients. Targeting CREB1 may be a potential tool for the diagnosis and treatment of OV.

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Evaluation of the expression of the immunosuppressive enzyme – indoleamine 2,3-dioxygenase in ovarian cancer tissue

Menopausal Review ◽

10.5114/pm.2013.36587 ◽

2013 ◽

Vol 3 ◽

pp. 223-227

Author(s):

Ewelina Rogala ◽

Aldona Nowicka ◽

Wiesława Bednarek ◽

Bartłomiej Barczyński ◽

Wanda Piekarczyk ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Tissue ◽

Ovarian Cancer Tissue ◽

Indoleamine 2,3 Dioxygenase

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Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS

Methods in Molecular Biology - Laser Capture Microdissection ◽

10.1007/978-1-61779-163-5_12 ◽

2011 ◽

pp. 155-163 ◽

Cited By ~ 9

Author(s):

Isabelle Cadron ◽

Toon Van Gorp ◽

Philippe Moerman ◽

Etienne Waelkens ◽

Ignace Vergote

Keyword(s):

Ovarian Cancer ◽

Proteomic Analysis ◽

Cancer Tissue ◽

Ovarian Cancer Tissue ◽

Tof Ms

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The feasibility of storing ovarian tumor cells on databasing paper: establishing a library of ovarian cancer DNA

International Journal of Gynecological Cancer ◽

10.1111/j.1525-1438.2006.00755.x ◽

2007 ◽

Vol 17 (1) ◽

pp. 94-100 ◽

Cited By ~ 8

Author(s):

K. Galaal ◽

M. Meirovitz ◽

R. Hussain ◽

L. Allcroft ◽

N. Sullivan ◽

...

Keyword(s):

Ovarian Cancer ◽

Cell Suspension ◽

Direct Sequencing ◽

Genetic Material ◽

Cell Number ◽

Pcr Analysis ◽

Tissue Banks ◽

Cancer Tissue ◽

Ovarian Cancer Tissue ◽

The Stability

The purpose of this study was to assess the feasibility of establishing a library of ovarian cancer nucleic acids using paper matrix by: 1) confirming the stability of DNA stored on paper matrix over a prolonged period of time, 2) determining the amount of genetic material required for storage, and 3) establishing the stability of RNA. Tumor tissue from 66 patients with ovarian cancer was collected intraoperatively, frozen, and dissociated with collagenase and trypsin. A cell suspension was then prepared and spotted onto the paper. The numbers of cells that were stored on the paper were counted using a hemocytometer. The cell suspension was serially diluted and spotted on the paper matrix until the minimum cell number that can be stored and produce a PCR product was determined. PCR, STR genotyping and direct sequencing were performed on tissue stored on the paper matrix. FTA® paper was used as RNA template, and RT PCR converted the RNA to cDNA. Ten to 50 mg of ovarian cancer tissue was stored on FTA® paper. We stored 7 × 104 cells on ISOcode® paper and 18 × 104 cells on FTA® and obtained extractable DNA. PCR analysis on cards with DNA stored 18 months ago enabled us to establish the stability of DNA after storage. RNA was stable for 6 months when stored on FTA® cards. Since genetic material is extractable from the paper matrices after passage of time, it could be a suitable medium for the storage of genetic material in cancer tissue banks.

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Presence of Mycoplasma DNA in Ovarian Cancer Tissue: Detection by PCR-ELISA Technique

Korean Journal of Gynecologic Oncology and Colposcopy ◽

10.3802/kjgoc.1998.9.1.70 ◽

1998 ◽

Vol 9 (1) ◽

pp. 70

Author(s):

Jong Hyeok Kim ◽

Jun Hee Na ◽

Myung Hee Lee ◽

Jae Young Um ◽

Yong Man Kim ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Tissue ◽

Ovarian Cancer Tissue ◽

Elisa Technique

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Novel ex vivo ovarian cancer tissue explant assay for prediction of chemosensitivity and response to novel therapeutics

Cancer Letters ◽

10.1016/j.canlet.2018.02.006 ◽

2018 ◽

Vol 421 ◽

pp. 51-58 ◽

Cited By ~ 12

Author(s):

Carmela Ricciardelli ◽

Noor A. Lokman ◽

Ilhamjan Sabit ◽

Kavyadharshini Gunasegaran ◽

Wendy M. Bonner ◽

...

Keyword(s):

Ovarian Cancer ◽

Ex Vivo ◽

Cancer Tissue ◽

Novel Therapeutics ◽

Ovarian Cancer Tissue

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Abstract 34: Predictors of discordance between image-based and manual scoring of immunohistochemical stains in ovarian cancer tissue microarrays

10.1158/1538-7445.am2020-34 ◽

2020 ◽

Author(s):

Naoko Sasamoto ◽

Mary Townsend ◽

Farnoosh Abbas-Aghababazadeh ◽

Kathryn L. Terry ◽

Joseph O. Johnson ◽

...

Keyword(s):

Ovarian Cancer ◽

Tissue Microarrays ◽

Cancer Tissue ◽

Ovarian Cancer Tissue ◽

Immunohistochemical Stains

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Critical role of SEMA5A expression in invasion and metastasis of ovarian cancer cell

American Journal of BioMedicine ◽

10.18081/2333-5106/014-04/247-259 ◽

2014 ◽

Vol 2 (4) ◽

pp. 247-259

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Cancer Cell ◽

Ovarian Cancer Cell ◽

Critical Role ◽

Migration And Invasion ◽

Ovarian Cancer Cells ◽

Cancer Tissue ◽

Invasion And Metastasis

Semaphorins are a large family of genes involved in the development and morphogenesis of the nervous system. SEMA5A has been reported as a bi-functional molecule, acting as both oncogene and tumor suppressor in different types of cancer. High expression levels of SEMA5A and its receptor, Plexin-B3, were associated with aggressiveness in pancreatic and prostate cancers. Our previous study in ovarian cancer metastasis indicates that FAK knock-down can suppress ovarian cancer cells migration and invasion. We hypothesized that SEMA5A expression promotes ovarian cancer invasion and metastasis. We investigated the expression of SEMA5A in patients with metastatic ovarian cancer (n = 43), localized tumor (n = 37) and normal ovarian tissue (n = 12) from non-malignant diseases as control with different histopathological characteristics. For Silencing of SEMA5A in vitro, we treated human ovarian cancer cells (OVCAR-3, A2780/CP70) with miR-27a and miR-27b. We observed significantly higher expression of SEMA5A protein (P= 0.001) in metastatic ovarian cancer tissue associated with poor overall survival outcomes compared to localized ovarian cancer and control. In vitro silencing of SEMA5A reduced migration and invasion of ovarian cancer cell. Our data offer opportunities for the therapeutic modulation and biomarker of metastatic ovarian cancer.

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