scholarly journals Metastatic myxosarcoma in a Quarter Horse gelding

2017 ◽  
Vol 30 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Jonathan P. Samuelson ◽  
Kate O. Echeverria ◽  
Jonathan H. Foreman ◽  
Richard L. Fredrickson ◽  
Deborah Sauberli ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Histochemical Staining ◽  
Periodic Acid Schiff ◽  
Abdominal Ultrasonography ◽  
University Of Illinois ◽  
Pleural Surface ◽  
Quarter Horse ◽  
The Masses ◽  
The University ◽  
Strong Immunoreactivity

A 22-y-old Quarter Horse gelding was presented to the University of Illinois Veterinary Teaching Hospital for evaluation of increased heart rate and mild colic signs. Rectal examination revealed a large left perirenal mass. Abdominal ultrasonography further confirmed this finding. Thoracic ultrasonography indicated multifocal irregularities on the pleural surface suggestive of consolidation and possibly masses in the lungs. The animal was euthanized. Autopsy findings included a large, firm, expansile, gelatinous retroperitoneal mass that surrounded both kidneys, as well as nodules with similar morphology in the lungs, liver, intestinal mesentery, cecum, and caudal mesenteric artery. Histologically, the masses were composed of neoplastic stellate-to-spindloid cells in abundant mucinous stroma. Neoplastic cells exhibited strong immunoreactivity for vimentin and were negative for pancytokeratin (A1/A3), CD3, CD20, melan A, and synaptophysin. Mucinous stroma was strongly positive with alcian blue and weakly positive with periodic acid–Schiff histochemical staining. These findings are consistent with metastatic myxosarcoma. Myxosarcoma is a rare neoplasm in horses, and metastasis to tissues other than sentinel lymph nodes has not been described previously to our knowledge.

scholarly journals  Histological and histochemical studies on the Harderian gland in native chickens

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 404-409 ◽  
Author(s):  
B. Mobini
Keyword(s):  
Epithelial Cells ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Harderian Gland ◽  
Histochemical Staining ◽  
Alveolar Type ◽  
Periodic Acid Schiff ◽  
Parasympathetic Ganglia ◽  
Tissue Sections ◽  
Native Chickens

  The objective of this investigation was to study the histological and histochemical structure of the Harderian gland in native chickens. Samples were obtained from 10 male and 10 female adult healthy native chickens. Tissue sections were stained with haematoxylin eosin, Verhoeff’s, Masson’s trichrome, alcian blue (pH 2.5), periodic acid-Schiff and Gomori’s method for reticulum. The multilobular Harderian gland of native chickens was covered by a thin connective tissue which consisted of adipose tissue, parasympathetic ganglia, nerve bundles, collagen, elastic and reticular fibres. Plasma cells were present in interlobular areas. The Harderian gland was compound tubulo-alveolar type. The Harderian duct was lined by columnar epithelial cells of varying height. Goblet cells were not found in Harderian duct. Histochemical staining revealed that the all epithelial cells of both corpus glandulae and ducts contained both neutral and acidic mucins. No significant sex-based differences were found. It is concluded that the general histological and histochemical structure of the Harderian gland in native chickens is similar to that of domestic geese, but that there are also some differences.  

scholarly journals HUMAN PITUITARY LUTEINIZING HORMONE: AN IMMUNOHISTOCHEMICAL STUDY

1966 ◽  
Vol 14 (2) ◽  
pp. 159-166 ◽  
Author(s):  
A. REES MIDGLEY
Keyword(s):  
Luteinizing Hormone ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Histochemical Staining ◽  
Performic Acid ◽  
Pars Tuberalis ◽  
Human Pituitary ◽  
Periodic Acid Schiff ◽  
Human Pituitary Gland ◽  
Aldehyde Fuchsin

Luteinizing hormone (LH) was localized immunohistochemically to cells in the pars anterior and pars tuberalis of the human pituitary gland. Upon restaining with a variety of histochemical procedures, LH-containing cells were found to be aldehyde fuchsin and periodic acid-Schiff (PAS) positive; however, the intensity of histochemical staining failed to correlate with that of fluorescence. Only a few of the PAS- or aldehyde fuchsin-positive cells contained LH. In contrast to the above, with the performic acid-alcian blue-PAS-organge G sequence of Adams, all cells containing LH were turquoise colored, alcian blue-positive (S1 mucoid cells) and vice versa. This study was performed with antiserum specific for human chorionic gonadotropin which was shown to cross react in immunodiffusion analysis with purified human pituitary luteinizing hormone and to be capable of neutralizing its biological activity.

Testicular Yolk Sac Carcinoma in a Calf

1998 ◽  
Vol 35 (3) ◽  
pp. 220-222 ◽  
Author(s):  
Y. Kagawa ◽  
A. Ohosaki ◽  
R. Ohosaki ◽  
O. Katsuta ◽  
M. Tsuchitani ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Periodic Acid ◽  
Abdominal Cavity ◽  
Domestic Animal ◽  
Yolk Sac ◽  
Pelvic Cavity ◽  
Placental Alkaline Phosphatase ◽  
Periodic Acid Schiff ◽  
First Case ◽  
The Masses

A testicular yolk sac carcinoma (YSCA) was diagnosed in a 28-day-old male Japanese black calf. Macroscopically, the abdominal cavity was filled with reddish gelatinous masses of various sizes. There were no testes in the scrotum or pelvic cavity. Histologically, the masses consisted of the tissues showing a variety of patterns; loose reticular network, pseudopapillary arrangement, festoon, solid nest, and labyrinthine pattern. Tumor cells were round to oval, with single central or polar nuclei with sharply defined nuclear borders and deeply basophilic chromatin. Tumor cells sometimes had glycogen granules, periodic acid–Schiff (PAS)-positive inclusions, or sudanophilic droplets in their cytoplasm. Hyaline matrices were found in extracellular areas and were intensely PAS positive. Immunohistochemically, most of the tumor cells and hyaline matrices had positive reactivity to anti-alpha-fetoprotein and anti-placental-alkaline-phosphatase antibodies. Positive reaction to anti-laminin antibody appeared only in hyaline matrices. This is the first case of a tumor in a domestic animal with histologic and immunohistochemical features analogous to those of human YSCA, endodermal sinus type.

scholarly journals The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

2021 ◽  
Vol 22 (13) ◽  
pp. 7131
Author(s):  
Maciej Witkowski ◽  
Laura Pardyak ◽  
Piotr Pawlicki ◽  
Anna Galuszka ◽  
Magdalena Profaska-Szymik ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
G Protein ◽  
Acid Content ◽  
Periodic Acid ◽  
Histochemical Staining ◽  
Intracellular Camp ◽  
Periodic Acid Schiff ◽  
Polysaccharide Content ◽  
Cryptorchid Testis ◽  
G Protein Coupled

Cryptorchidism in horses is a commonly occurring malformation. The molecular basis of this pathology is not fully known. In addition, the origins of high intratesticular estrogen levels in horses remain obscure. In order to investigate the role of the G-protein-coupled membrane estrogen receptor (GPER) and establish histological and biochemical cryptorchid testis status, healthy and cryptorchid horse testes were subjected to scanning electron microscopy analysis, histochemical staining for total protein (with naphthol blue black; NBB), acid content (with toluidine blue O; TBO), and polysaccharide content (with periodic acid–Schiff; PAS). The expression of GPER was analyzed by immunohistochemistry and Western blot. GPER-mediated intracellular cAMP and calcium (Ca2+) signaling were measured immunoenzymatically or colorimetrically. Our data revealed changes in the distribution of polysaccharide content but not the protein and acid content in the cryptorchid testis. Polysaccharides seemed to be partially translocated from the interstitial compartment to the seminiferous tubule compartment. Moreover, the markedly decreased expression of GPER and GPER downstream molecules, cAMP and Ca2+, suggests their potential role in testis pathology. Increased estrogen levels in cryptorchid conditions may be linked to disturbed GPER signaling. We postulate that GPER is a prominent key player in testis development and function and may be used as a new biomarker of horse testis in health and disease.

Polysaccharide Storage Myopathy in Morgan, Arabian, and Standardbred Related Horses and Welsh-cross Ponies

2000 ◽  
Vol 37 (2) ◽  
pp. 193-196 ◽  
Author(s):  
B. A. Valentine ◽  
S. P. McDonough ◽  
Y.-F. Chang ◽  
A. J. Vonderchek
Keyword(s):  
Skeletal Muscle ◽  
Periodic Acid ◽  
Clinical Signs ◽  
Neuromuscular Disorder ◽  
Periodic Acid Schiff ◽  
Exertional Rhabdomyolysis ◽  
Polysaccharide Storage Myopathy ◽  
Quarter Horse ◽  
Criteria For Diagnosis ◽  
Progressive Weakness

Polysaccharide storage myopathy is an equine neuromuscular disorder characterized by accumulation of glycogen-related polysaccharide inclusions within skeletal muscle fibers. The pathologic criteria for diagnosis of this disorder are somewhat controversial; however, periodic acid-Schiff-positive, amylase-resistant inclusions are considered pathognomonic. Although these inclusions are most often found in affected horses related to the Quarter Horse, draft horse, and Warmblood breeds, this report describes these characteristic inclusions in muscle of five horses from nonrelated breeds (two Morgans, one Arabian, one Arabian X Thoroughbred, and one Standardbred) and two Welsh cross ponies. Affected horses had histories of recurrent exertional rhabdomyolysis, and one developed progressive weakness leading to increased recumbency. The affected ponies were part of an unrelated research project and had no apparent clinical signs.

scholarly journals Histomorphological and histochemical study of stomach of domestic pigeon (Columba livia domestica)

2016 ◽  
Vol 40 (1) ◽  
pp. 89-96
Author(s):  
F. J. Al-Saffar
Keyword(s):  
Periodic Acid ◽  
Smooth Muscles ◽  
Columba Livia ◽  
Histochemical Staining ◽  
Fatty Tissue ◽  
Muscularis Mucosa ◽  
Periodic Acid Schiff ◽  
Domestic Pigeon ◽  
Internal Lining ◽  
Mucosal Glands

     The study aimed at describing the histomorphological and histochemical structures of the stomach wall in domestic pigeon. Twelve healthy birds were collected from local suppliers at Baghdad province. Birds were divided into three equal groups for gross, histological and histochemical approaches. All birds were euthanized and their abdomens were dissected, then their stomachs were identified and photographed in situ. Specimens from stomach were fixed as well and then processed for histological and histochemical staining techniques using Hematoxylin-eosin, Masson Trichrome, Periodic acid Schiff (PAS) and PAS -Alcian blue (AB) (pH 2.5). Grossly the stomach of the pigeon consisted of two distinguishable chambers, that were glandular or proventriculus and muscular or ventriculus. The proventriculus was tubular-shaped organ. Whereas, the ventriculus appeared fusiform in shape and surrounded by considerable amount of fatty tissue. Histologically the proventriculus mucosal lining was simple columnar epithelium cells. The submucosal consists of numerous and voluminous deep submucosal glands which occupied the greater part of the organ wall. The internal lining of the ventriculus was simple columnar cells. Muscularis mucosa appeared circularly arranged bundles interrupted by the presence of mucosal glands in the lamina propria. The tunica muscularis appeared as a very thick structure of smooth muscles fibers of three layers, thin inner, outer longitudinal and very thick intermediate circular layers.

Bovine Myocardial Epithelial Inclusions

1993 ◽  
Vol 30 (1) ◽  
pp. 82-88 ◽  
Author(s):  
D. C. Baker ◽  
S. P. Schmidt ◽  
K. A. Langheinrich ◽  
L. Cannon ◽  
R. A. Smart
Keyword(s):  
Epithelial Cells ◽  
Periodic Acid ◽  
Intercellular Junctions ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Colloidal Iron ◽  
Periodic Acid Schiff ◽  
Holstein Calves ◽  
The Masses ◽  
Formalin Fixed

Light microscopic, histochemical, immunohistochemical, and ultrastructural methods were used to examine myocardial epithelial masses in the hearts of ten cattle. The tissues consisted of paraffin-embedded or formalin-fixed samples from eight hearts that were being inspected in slaughter houses and from two hearts from calves that died of septicemia. The ages of the cattle ranged from 4 days to 12 years; the breeds were unspecified for all but one Hereford female and the two Holstein calves; and there were three males, four females, and three steers. The masses in these cases were compared with similar appearing lesions found in other animal species. The lesions in the bovine hearts were single to multiple, well circumscribed, found in the left ventricle wall, and composed of squamous to cuboidal epithelial cells that formed tubular, ductular, and acinar structures with lumens that were void or filled with amorphous protein globules. Electron microscopic examination revealed epithelial cells that had sparse apical microvilli, tight apical intercellular junctions, perinuclear bundles of filaments, and rare cilia. Almost half of the bovine epithelial masses (4/9) had occasional diastase-resistant periodic acid-Schiff-positive granules in their cytoplasm, and few had hyaluronidase-resistant alcian blue-positive granules (2/9) or colloidal iron-positive granules (1/9). All myocardial masses had abundant collagen surrounding the tubular and acinar structures, and 2/9 had elastin fibers as well. None of the myocardial masses had Churukian-Schenk or Fontana Masson's silver staining granules in epithelial cells. Immunohistochemically, all bovine myocardial tumors stained positively for cytokeratin (8/8), and occasional masses stained positively for vimentin (3/8) or carcinoembryonic antigen (3/8). None of the masses stained positively for desmin. The myocardial epithelial tumors most likely represent endodermal rests of tissue misplaced during organogenesis.

Pseudofungi in Pericolic Lymph Nodes

2005 ◽  
Vol 129 (4) ◽  
pp. e97-e100 ◽  
Author(s):  
Dong Eun Song ◽  
Andrea G. Kahn ◽  
Shin Kwang Khang ◽  
Jae Y. Ro
Keyword(s):  
Lymph Nodes ◽  
Periodic Acid ◽  
Electron Microscopic Examination ◽  
Pelvic Cavity ◽  
Clinical Impression ◽  
Positive Staining ◽  
Electron Microscopic ◽  
Periodic Acid Schiff ◽  
Dense Granules ◽  
The Masses

Abstract We report a case of extensive pseudofungi in the pericolic lymph nodes. A 45-year-old man presented with lower abdominal discomfort, and large solid and cystic masses were discovered in the pelvic cavity by computed tomography. Debulking of the masses (by means of a Hartmann operation) was performed under the clinical impression of a pelvic sarcoma, and histologically the masses demonstrated features of a malignant gastrointestinal stromal tumor. All pericolic lymph nodes demonstrated multiple septate hyphae–like structures with pigmentation, particularly in the subcapsular sinuses. These structures showed positive staining with periodic acid–Schiff, but Gomori methenamine silver staining was negative. The structures appeared to be composed of iron, phosphorus, and calcium by Perls iron and von Kossa stains and revealed deposition of electron-dense granules on electron microscopic examination. Energy-dispersive radiographic micro-analysis showed the structures to contain phosphorus, sulfur, potassium, calcium, and iron. Pseudofungi can be misinterpreted as true fungi with septate hyphae; therefore, a careful morphologic examination with appropriate special stains is mandatory.

Multicentric Benign Peripheral Nerve Sheath Tumors in Two Related Bearded Dragons, Pogona vitticeps

2005 ◽  
Vol 42 (4) ◽  
pp. 507-510 ◽  
Author(s):  
K. Y. Lemberger ◽  
A. Manharth ◽  
A. P. Pessier
Keyword(s):  
Peripheral Nerve ◽  
Smooth Muscle Actin ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Left Shoulder ◽  
Periodic Acid Schiff ◽  
Peripheral Nerve Sheath Tumors ◽  
Nerve Sheath ◽  
Pogona Vitticeps ◽  
The Masses

Multiple subcutaneous masses from two sibling bearded dragons were removed. Nodules were well demarcated, restricted to the subcutis, and soft, white to yellow, resembling adipose tissue. Histologically, the masses were composed of short interlacing streams and bundles of spindle cells, with regions of vague nuclear palisading. Two of the tumors contained a subpopulation of polygonal cells with abundant periodic acid-Schiff (PAS)-positive cytoplasmic granules. Neoplastic cells were immunohistochemically positive for S100 and neuron-specific enolase (NSE) but negative for desmin and smooth muscle actin. Electron microscopy and reticulin stains demonstrated a continuous basal lamina separating intertwining cells. Histologic, ultrastructural, and immunohistochemical features were consistent with a peripheral nerve sheath origin. At 1 year postexcision, local reoccurrence of a single incompletely excised mass from the left shoulder was noted.

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