scholarly journals Histomorphological and histochemical study of stomach of domestic pigeon (Columba livia domestica)

2016 ◽  
Vol 40 (1) ◽  
pp. 89-96
Author(s):  
F. J. Al-Saffar
Keyword(s):  
Periodic Acid ◽  
Smooth Muscles ◽  
Columba Livia ◽  
Histochemical Staining ◽  
Fatty Tissue ◽  
Muscularis Mucosa ◽  
Periodic Acid Schiff ◽  
Domestic Pigeon ◽  
Internal Lining ◽  
Mucosal Glands

     The study aimed at describing the histomorphological and histochemical structures of the stomach wall in domestic pigeon. Twelve healthy birds were collected from local suppliers at Baghdad province. Birds were divided into three equal groups for gross, histological and histochemical approaches. All birds were euthanized and their abdomens were dissected, then their stomachs were identified and photographed in situ. Specimens from stomach were fixed as well and then processed for histological and histochemical staining techniques using Hematoxylin-eosin, Masson Trichrome, Periodic acid Schiff (PAS) and PAS -Alcian blue (AB) (pH 2.5). Grossly the stomach of the pigeon consisted of two distinguishable chambers, that were glandular or proventriculus and muscular or ventriculus. The proventriculus was tubular-shaped organ. Whereas, the ventriculus appeared fusiform in shape and surrounded by considerable amount of fatty tissue. Histologically the proventriculus mucosal lining was simple columnar epithelium cells. The submucosal consists of numerous and voluminous deep submucosal glands which occupied the greater part of the organ wall. The internal lining of the ventriculus was simple columnar cells. Muscularis mucosa appeared circularly arranged bundles interrupted by the presence of mucosal glands in the lamina propria. The tunica muscularis appeared as a very thick structure of smooth muscles fibers of three layers, thin inner, outer longitudinal and very thick intermediate circular layers.

scholarly journals Brown Bowel Syndrome: An Exceedingly Rare Condition with Longstanding Malabsorption and an Unusual Cause of Colon Pseudo-Obstruction

2021 ◽  
pp. 960-965
Author(s):  
Adnan Alkurdi ◽  
Diana Rubin ◽  
Alexander Seelhoff ◽  
Hermann Herbst
Keyword(s):  
Vitamin E ◽  
Periodic Acid ◽  
Clinical Status ◽  
Smooth Muscles ◽  
Rare Condition ◽  
Underlying Disease ◽  
High Dose ◽  
Muscularis Mucosa ◽  
Periodic Acid Schiff ◽  
Lipofuscin Granules

Brown bowel syndrome (BBS) is an exceedingly rare condition usually associated with longstanding malabsorption of any etiology. As a result of vitamin E deficiency and subsequent mitochondrial degeneration due to oxidative stress induced by free radicals, lipofuscin granules accumulate in the smooth muscles of the gastrointestinal tract resulting in myopathy and dysmotility with underlying disease aggravation. The current study reports a BBS case in a 64-year-old female patient who had undergone jejunoileal bypass surgery as a bariatric procedure. The patient was admitted with signs of malabsorption and ileus in computed tomography imaging. Endoscopic workup revealed no stenosis or obstruction. The colon histologically showed periodic acid-Schiff-positive lipofuscin granules in the lamina muscularis mucosa consistent with BBS. The vitamin E level in the patient was extremely low. Moreover, clinical improvement was documented following high-dose substitution. BBS should be considered in patients with malabsorption of any cause especially with signs of gastrointestinal dysmotility. Vitamin E substitution may improve clinical status and prevent further deterioration.

scholarly journals  Histological and histochemical studies on the Harderian gland in native chickens

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 404-409 ◽  
Author(s):  
B. Mobini
Keyword(s):  
Epithelial Cells ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Harderian Gland ◽  
Histochemical Staining ◽  
Alveolar Type ◽  
Periodic Acid Schiff ◽  
Parasympathetic Ganglia ◽  
Tissue Sections ◽  
Native Chickens

  The objective of this investigation was to study the histological and histochemical structure of the Harderian gland in native chickens. Samples were obtained from 10 male and 10 female adult healthy native chickens. Tissue sections were stained with haematoxylin eosin, Verhoeff’s, Masson’s trichrome, alcian blue (pH 2.5), periodic acid-Schiff and Gomori’s method for reticulum. The multilobular Harderian gland of native chickens was covered by a thin connective tissue which consisted of adipose tissue, parasympathetic ganglia, nerve bundles, collagen, elastic and reticular fibres. Plasma cells were present in interlobular areas. The Harderian gland was compound tubulo-alveolar type. The Harderian duct was lined by columnar epithelial cells of varying height. Goblet cells were not found in Harderian duct. Histochemical staining revealed that the all epithelial cells of both corpus glandulae and ducts contained both neutral and acidic mucins. No significant sex-based differences were found. It is concluded that the general histological and histochemical structure of the Harderian gland in native chickens is similar to that of domestic geese, but that there are also some differences.  

scholarly journals HUMAN PITUITARY LUTEINIZING HORMONE: AN IMMUNOHISTOCHEMICAL STUDY

1966 ◽  
Vol 14 (2) ◽  
pp. 159-166 ◽  
Author(s):  
A. REES MIDGLEY
Keyword(s):  
Luteinizing Hormone ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Histochemical Staining ◽  
Performic Acid ◽  
Pars Tuberalis ◽  
Human Pituitary ◽  
Periodic Acid Schiff ◽  
Human Pituitary Gland ◽  
Aldehyde Fuchsin

Luteinizing hormone (LH) was localized immunohistochemically to cells in the pars anterior and pars tuberalis of the human pituitary gland. Upon restaining with a variety of histochemical procedures, LH-containing cells were found to be aldehyde fuchsin and periodic acid-Schiff (PAS) positive; however, the intensity of histochemical staining failed to correlate with that of fluorescence. Only a few of the PAS- or aldehyde fuchsin-positive cells contained LH. In contrast to the above, with the performic acid-alcian blue-PAS-organge G sequence of Adams, all cells containing LH were turquoise colored, alcian blue-positive (S1 mucoid cells) and vice versa. This study was performed with antiserum specific for human chorionic gonadotropin which was shown to cross react in immunodiffusion analysis with purified human pituitary luteinizing hormone and to be capable of neutralizing its biological activity.

scholarly journals Metastatic myxosarcoma in a Quarter Horse gelding

2017 ◽  
Vol 30 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Jonathan P. Samuelson ◽  
Kate O. Echeverria ◽  
Jonathan H. Foreman ◽  
Richard L. Fredrickson ◽  
Deborah Sauberli ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Histochemical Staining ◽  
Periodic Acid Schiff ◽  
Abdominal Ultrasonography ◽  
University Of Illinois ◽  
Pleural Surface ◽  
Quarter Horse ◽  
The Masses ◽  
The University ◽  
Strong Immunoreactivity

A 22-y-old Quarter Horse gelding was presented to the University of Illinois Veterinary Teaching Hospital for evaluation of increased heart rate and mild colic signs. Rectal examination revealed a large left perirenal mass. Abdominal ultrasonography further confirmed this finding. Thoracic ultrasonography indicated multifocal irregularities on the pleural surface suggestive of consolidation and possibly masses in the lungs. The animal was euthanized. Autopsy findings included a large, firm, expansile, gelatinous retroperitoneal mass that surrounded both kidneys, as well as nodules with similar morphology in the lungs, liver, intestinal mesentery, cecum, and caudal mesenteric artery. Histologically, the masses were composed of neoplastic stellate-to-spindloid cells in abundant mucinous stroma. Neoplastic cells exhibited strong immunoreactivity for vimentin and were negative for pancytokeratin (A1/A3), CD3, CD20, melan A, and synaptophysin. Mucinous stroma was strongly positive with alcian blue and weakly positive with periodic acid–Schiff histochemical staining. These findings are consistent with metastatic myxosarcoma. Myxosarcoma is a rare neoplasm in horses, and metastasis to tissues other than sentinel lymph nodes has not been described previously to our knowledge.

scholarly journals Histological and Histochemical studies of Esophagus in Mabuya aurata septemaeniata

2015 ◽  
Vol 12 (3) ◽  
pp. 437-444
Author(s):  
Baghdad Science Journal
Keyword(s):  
Epithelial Cells ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Muscularis Mucosa ◽  
Periodic Acid Schiff

The wall of the esophagus in Mabuya aurata septemaeniata as in high vertebrates consists of four layers, mucosa, submucosa,muscularis and serosa. Mucosa forms many unorganized short and long folds penetrate inside the esophageal cavity. Mucosa contains two sub layers, first one is lining epithelium which includes two types of cells, simple ciliated columnar epithelial cells and goblet cells, second one is lamina properia. Mucosa does not have muscularis mucosa. There is no esophageal glands within esophagus. Many special stains were used as (Periodic Acid Schiff (PAS)) to detect Carbohydrates in goblet cells. Alcian blue were used to detect the amount of goblet cells within lining epithelium. Alcian blue + PAS together confirm that the secreted mucin from goblet cells was acidic.

scholarly journals Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas

2021 ◽  
Vol 21 (2) ◽  
pp. 128-140
Author(s):  
Bagus Dwijayanti ◽  
Erdiansyah Rahmi ◽  
Ummu Balqis ◽  
Fitriani Fitriani ◽  
Dian Masyitha ◽  
...  
Keyword(s):  
Incubation Period ◽  
Periodic Acid ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Muscularis Mucosa ◽  
Gallus Gallus Domesticus ◽  
Periodic Acid Schiff ◽  
Chicken Intestine ◽  
Native Chicken ◽  
Significant Difference

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P˂0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P˂0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7.  (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P˂0.05) on macroscopic morphometry of the native chicken’s intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P˂0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.

scholarly journals The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

2021 ◽  
Vol 22 (13) ◽  
pp. 7131
Author(s):  
Maciej Witkowski ◽  
Laura Pardyak ◽  
Piotr Pawlicki ◽  
Anna Galuszka ◽  
Magdalena Profaska-Szymik ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
G Protein ◽  
Acid Content ◽  
Periodic Acid ◽  
Histochemical Staining ◽  
Intracellular Camp ◽  
Periodic Acid Schiff ◽  
Polysaccharide Content ◽  
Cryptorchid Testis ◽  
G Protein Coupled

Cryptorchidism in horses is a commonly occurring malformation. The molecular basis of this pathology is not fully known. In addition, the origins of high intratesticular estrogen levels in horses remain obscure. In order to investigate the role of the G-protein-coupled membrane estrogen receptor (GPER) and establish histological and biochemical cryptorchid testis status, healthy and cryptorchid horse testes were subjected to scanning electron microscopy analysis, histochemical staining for total protein (with naphthol blue black; NBB), acid content (with toluidine blue O; TBO), and polysaccharide content (with periodic acid–Schiff; PAS). The expression of GPER was analyzed by immunohistochemistry and Western blot. GPER-mediated intracellular cAMP and calcium (Ca2+) signaling were measured immunoenzymatically or colorimetrically. Our data revealed changes in the distribution of polysaccharide content but not the protein and acid content in the cryptorchid testis. Polysaccharides seemed to be partially translocated from the interstitial compartment to the seminiferous tubule compartment. Moreover, the markedly decreased expression of GPER and GPER downstream molecules, cAMP and Ca2+, suggests their potential role in testis pathology. Increased estrogen levels in cryptorchid conditions may be linked to disturbed GPER signaling. We postulate that GPER is a prominent key player in testis development and function and may be used as a new biomarker of horse testis in health and disease.

scholarly journals Idiopathic Complex Polysaccharide Storage Disease in an Abyssinian Cat

2005 ◽  
Vol 42 (4) ◽  
pp. 502-506 ◽  
Author(s):  
I. M. Langohr ◽  
M. Tanabe
Keyword(s):  
Storage Disease ◽  
Periodic Acid ◽  
Glycogen Storage ◽  
Histochemical Staining ◽  
Periodic Acid Schiff ◽  
Pathologic Process ◽  
Transmission Electron ◽  
History Of ◽  
Hematoxylin And Eosin ◽  
Filamentous Material

A glycogen storage disease affecting primarily the skeletal muscle and, to a lesser degree, the cardiac muscle, spinal cord, and brain was diagnosed in a 10-year-old neutered Abyssinian cat with a 4-year history of paresis progressing to acute paralysis. Microscopically, these tissues contained inclusions that were pale basophilic in hematoxylin and eosin-stained slides, diastase resistant, periodic acid-Schiff positive, and blue-to-almost black with iodine stain. By transmission electron microscopy, the inclusions consisted of cytosolic, usually sharply demarcated, nonmembrane-bound deposits of finely granular and filamentous material. on the basis of the structural and histochemical staining characteristics, the inclusions were believed to be aggregates of abnormally stored, unbranched glycogen. A defect in glucose metabolism is suspected to be the underlying pathologic process, but an exact cause remains elusive.

scholarly journals Histochemical Study of Human Placental Tissues in Gestational Diabetic Mellitus

2021 ◽  
Vol 10 (2) ◽  
pp. 29-38
Author(s):  
Israa Lafta ◽  
Wasan Abdulhameed ◽  
Nahla AL-Bakri
Keyword(s):  
Gestational Diabetes ◽  
Periodic Acid ◽  
Glycogen Metabolism ◽  
Histochemical Staining ◽  
Basement Membranes ◽  
Diabetic Mellitus ◽  
Periodic Acid Schiff ◽  
Chronic Hyperglycemia ◽  
Glycogen Deposition ◽  
Fetal Vessels

Gestational diabetes mellitus (GDM) is a serious pregnancy complication in which a woman who has never had diabetes develops chronic hyperglycemia during her pregnancy. Normal placental function is essential for optimal fetal growth. The transport of glucose from the mother to the fetus is critical for fetal nutrient demands and can be stored as glycogen in the placenta. However, the function of this glycogen deposition is unknown: It may well be a source of fuel for a placenta itself or the storage reservoir for the later use by the fetus in times of need. While the significance of the placental glycogen remains unknown, the mounting evidence indicates that the altered glycogen metabolism and/or deposition is associated with many pregnancy complications, such as gestational diabetes, that adversely affect fetal development. The aim of this study is to assess glycogen deposition using Histochemical staining of Periodic Acid Schiff (PAS) stain. The placenta tissue collected from 50 women were enrolled in this study (25 women with no complications) and (25 women with gestational diabetes). The placentas of the two groups were compared in this study based on glycogen deposition with periodic acid-Schiff stain. The results of a histochemical investigation using PAS stain revealed a significant increase in the glycogen deposition (p≤0.001) in diabetic women's placentas within the intervillous core, around fetal vessels, and the basement membranes.

scholarly journals Clinico-morphological Profile of Cutaneous Fungal Infections: An Experience from a Tertiary Care Government Hospital in North India

2021 ◽  
Vol 8 (2) ◽  
pp. A56-62
Author(s):  
Ekta Jain ◽  
Rajpal Singh Punia ◽  
Jagdish Chander ◽  
Mala Bhalla
Keyword(s):  
Fungal Infections ◽  
Periodic Acid ◽  
Critical Role ◽  
Tertiary Care ◽  
Rapid Identification ◽  
Histochemical Staining ◽  
North India ◽  
Fungal Culture ◽  
Periodic Acid Schiff ◽  
Fixed Tissue

Background: Cutaneous fungal infections are predominantly seen in hot tropical countries like India. In the past decade, there has been an escalation in recurrent and chronic fungal infections. Skin biopsy may play a critical role in rapid identification of these infections. Methods: Fifteen cases of cutaneous fungal infections over a period of 6 years were included. Formalin-fixed tissue was subjected to hematoxylin and eosin and histochemical staining including Gomori Methanamine Silver and Periodic Acid Schiff . Results of KOH smear test and fungal culture were included wherever available. The clinico-morphological patterns in various cutaneous fungal infections was evaluated. Results: Most patients were between 25 to 40 years of age and commonly presented as skin ulceration, followed by nodular swelling and multiple discharging sinuses. Candidiasis and Mycetoma infections were the commonest infections. Others included: Cryptococcosis, Dermatophytosis, Chromoblastomycosis and Mucormycosis. The predominant histopathologic patterns were perivascular and interstitial inflammation. Eleven cases were confirmed by KOH examination and culture. Conclusion: The morphologic spectrum of cutaneous fungal infections is varied. Initial presentations of these fungal infections may be indicative of the onset of a life-threatening systemic mycoses. Thus, the histopathologic evaluation of skin tissue specimens is critical for their rapid and accurate diagnosis.

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