Production of granulomas in Mycoplasma bovis infection associated with meningitis-meningoencephalitis, endocarditis, and pneumonia in cattle

Mycoplasma bovis, the most important primary pathogen in the family Mycoplasmataceae, causes pneumonia, arthritis, otitis media, and mastitis in cattle. Histopathologic pulmonary changes associated with M. bovis infection have been characterized as suppurative-to-caseonecrotic bronchopneumonia; infection in other organs has been reported in only a few studies that examined caseonecrotic endocarditis and suppurative meningitis. Granulomatous lesions associated with M. bovis infection have been reported only rarely. We studied the granulomatous inflammation associated with M. bovis infection in several organs of 21 Japanese Black cattle. M. bovis was detected by isolation and loop-mediated isothermal amplification methods; other bacteria were detected using culture on 5% blood sheep agar and a MALDI-TOF MS Biotyper. Tissues were examined by histopathology and by immunohistochemistry (IHC) using anti– M. bovis, anti-Iba1, anti-iNOS, and anti-CD204 antibodies. All 21 cases, which included 2 cases of meningitis-meningoencephalitis, 8 cases of endocarditis, and 11 cases of bronchopneumonia, had caseonecrotic granulomatous inflammation associated with M. bovis infection. The IHC for macrophages revealed a predominance of iNOS-labeled (M1) macrophages in the inner layer of the caseonecrotic granulomas associated with meningitis-meningoencephalitis, endocarditis, and bronchopneumonia in Japanese Black cattle naturally infected with M. bovis.

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Optimizing identification of Mycoplasma bovis by MALDI-TOF MS

Research in Veterinary Science ◽

10.1016/j.rvsc.2019.06.010 ◽

2019 ◽

Vol 125 ◽

pp. 185-188 ◽

Cited By ~ 6

Author(s):

Jade Bokma ◽

Bart Pardon ◽

Laura Van Driessche ◽

Linde Gille ◽

Piet Deprez ◽

...

Keyword(s):

Mycoplasma Bovis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Staphylococcus aureus and Pseudomonas aeruginosa in Tubotympanic Chronic Suppurative Otitis Media Patients in Purwokerto, Indonesia

The Indonesian Biomedical Journal ◽

10.18585/inabj.v12i4.1218 ◽

2020 ◽

Vol 12 (4) ◽

pp. 340-348

Author(s):

Daniel Joko Wahyono ◽

Anton Budhi Darmawan ◽

Leader Alfason ◽

Reinhard Simbolon ◽

Siwi Pramatama Mars Wijayanti ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Antimicrobial Susceptibility ◽

Chronic Suppurative Otitis Media ◽

Molecular Tools ◽

Suppurative Otitis Media ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

BACKGROUND: Chronic Suppurative Otitis Media (CSOM) causes hearing impairment and frequently occurred in low-income country where medical care and personal hygiene are poor. Staphylococcus aureus and Pseudomonas aeruginosa are the most common cause of CSOM. We investigated prevalence and antimicrobial susceptibility of S. aureus and P. aeruginosa from tubotympanic CSOM patients in tertiary hospital, Purwokerto, Indonesia in 2016-2017.METHODS: Ear swab specimens were collected from patients with tubotympanic CSOM. S. aureus and P. aeruginosa were isolated and identified by culture, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and molecular tools. Antimicrobial susceptibility testing was performed using the disk diffusion method.RESULTS: Out of ear swabs from 34 patients with tubotympanic CSOM, P. aeruginosa and S. aureus were identified in 35%patients. No Methicillin-resistant S. aureus (MRSA) strain was found from the ear swabs of the patients with tubotympanic CSOM. Bacterial identification using the MALDI-TOF MS was concordantly with culture and molecular tools. All S. aureus isolates showed full susceptibility to cefoxitin and trimethoprim-sulphamethoxazole. Resistance to tetracycline was common with only 64% of S. aureus strains being susceptible. Meanwhile, all P. aeruginosa strains were susceptible to cefepime, cetazidime, meropenem, gentamicin, and tobramycin.CONCLUSION: S. aureus and P. aeruginosa are found in patients with tubotympanic CSOM and still susceptible to different antibiotic agents. MALDI-TOF MS demonstrate rapid, accurate and robust to detect S. aureus and P. aeruginosa.KEYWORDS: Staphylococcus aureus, Pseudomonas aeruginosa, chronic tubotympanic suppurative otitis media

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MALDI-TOF mass spectrometry and high-resolution melting PCR for the identification of Mycoplasma bovis isolates

BMC Veterinary Research ◽

10.1186/s12917-021-02870-5 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Aric J. McDaniel ◽

Rachel J. Derscheid

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

High Resolution Melting ◽

Clinical Manifestations ◽

Mycoplasma Bovis ◽

Pcr Assay ◽

Accurate Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

Abstract Background Mycoplasma bovis is an important pathogen of cattle worldwide. Many different clinical manifestations of infection can occur, including respiratory disease, arthritis, and mastitis, causing heavy losses to beef and dairy industries. Because Mycoplasma species are slow-growing and fastidious, traditional identification methods are not cost- or time-effective, and improved methods are sought to streamline laboratory processes. High-resolution melting PCR (HRM-PCR) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) are 2 relatively recent tools that are rapid and inexpensive to use; we tested 9 isolates of M. bovis using both assays. The HRM-PCR assay used universal mycoplasma primers for the 16S–23S intergenic spacer region (IGSR). Results The resulting melting profiles of the field isolates were indistinguishable from the reference strain, indicating accurate identification. For the MALDI-TOF MS, each M. bovis isolate was accurately identified. Mycoplasma arginini and Mycoplasma alkalescens isolates did not identify as M. bovis when tested by either assay. Conclusions Our work shows that either assay could be used to identify unknown M. bovis isolates. For future work, the MALDI-TOF MS library should be expanded to include more mycoplasmas, and the HRM-PCR assay should be tested on additional mycoplasmas to ensure that the melting profiles are sufficiently distinctive.

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Granulomatous metritis caused by suspected Dirofilaria immitis in a dog: a case report

Veterinární Medicína ◽

10.17221/6984-vetmed ◽

2013 ◽

Vol 58 (No. 8) ◽

pp. 437-441

Author(s):

JK Park ◽

AY Kim ◽

EM Lee ◽

EJ Lee ◽

DM Kwak ◽

...

Keyword(s):

Case Report ◽

Soft Tissue ◽

Dirofilaria Immitis ◽

Vaginal Discharge ◽

Granulomatous Inflammation ◽

Unknown Origin ◽

Uterine Horn ◽

The Family ◽

Granulomatous Lesions ◽

Abdominal Radiographs

Here we describe a unique uterine mass in a dog with granulomatous lesions caused by filarial larvae from the family Onchocercidae. An 8-year-old female Maltese was presented to a local animal hospital with anorexia, depression, and vaginal discharge. A markedly distended uterus was observed on lateral abdominal radiographs, leading to a clinical diagnosis of pyometra or uterine mass of an unknown origin. During surgery, the left uterine horn contained a 5 cm diameter mass adhered to adjacent soft tissue. On gross inspection, the mass contained numerous white nematodes. Microscopically, this mass was characterised by a granulomatous inflammation of the myometrium and endometrium. Because all of the nematodes were dead, definitive species identification was not possible. However, based on the histologic appearance, these nematodes were tentatively identified as Dirofilaria immitis larvae.  

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