Intrachondral Microvasculature in the Human Fetal Talus

The intrachondral microvasculature of the growing talus of human was studied in 16 fetuses aged from 15 to 44 weeks of gestation, using interrupted serial sections and vascular injection of ink. The cartilage model of the talus was shown to be well vascularized throughout by cartilage canals. The cartilage canal contained blood vessels and connective tissue, with vessels originating from the perichondrial vessels. They were covered by a thick connective tissue wall that was continuous with the perichondrium. The functions of the cartilage canals were mainly to nourish the large masses of cartilage and to supply osteogenic tissue, which initiates the primary ossification center. As in the adult, the fetal talus was supplied with four to five main branches originating from the sinus tarsi and the tarsal canal; there were no anastomoses between the vessels of the adjacent cartilage canals and between the branches within the cartilage canal. This type of microvasculature is vulnerable to injury and, if impaired, may cause serious complications.

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Enamel Matrix Derivative in Diffusion Chamber Implanted Subcutaneously in Rat Induces Formation of Fibrous Connective Tissue Containing Abundant Blood Vessels

In Vivo ◽

10.21873/invivo.12261 ◽

2021 ◽

Vol 35 (1) ◽

pp. 313-317

Author(s):

SATOSHI YOKOSE ◽

YUKA KATO ◽

KATSUTOSHI MATSUMOTO ◽

PERRY R. KLOKKEVOLD ◽

HENRY H. TAKEI ◽

...

Keyword(s):

Connective Tissue ◽

Blood Vessels ◽

Diffusion Chamber ◽

Enamel Matrix Derivative ◽

Fibrous Connective Tissue ◽

Enamel Matrix ◽

Matrix Derivative

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Morphofunctional and immunohistochemical characteristics of the large omentum in ovarian cancer

Journal of Anatomy and Histopathology ◽

10.18499/2225-7357-2020-9-3-64-71 ◽

2020 ◽

Vol 9 (3) ◽

pp. 64-71

Author(s):

N. N. Shevlyuk ◽

L. V. Khalikova ◽

A. A. Khalikov

Keyword(s):

Ovarian Cancer ◽

Connective Tissue ◽

Protein Expression ◽

Blood Vessels ◽

Lymphoid Tissue ◽

P53 Protein ◽

Individual Variability ◽

The Body ◽

Great Omentum ◽

Low Grade

The aim of the study was to establish morphofunctional and immunohistochemical characteristics of large omentum in women with ovarian cancer.Material and methods. The large omenta of 48 women with ovarian cancer (low-grade differentiated seropapillary adenocarcinoma of high-grade malignancy) of II stage (n=20) and III stage (n=28) were studied. Histological sections were stained with overview histological and immunohistochemical methods (to reveal ki67, P53, CD34, CD7, CD4, CD8, CD61 proteins expression). Results. In patients, the size of the large omentum was characterized by high individual variability; in the presence of metastasis, the size of the omentum was reduced. Intensive development of blood vessels in the organ was noted, but in the presence of metastases stasis of blood corpuscles, leucocytic infiltration, and moderate edema of connective tissue were observed in the organ’s vessels. Areas of lymphoid tissue, both small lymphatic follicles and diffusely located lymphoid tissue, were revealed in the omentum. In most follicles, reactive centers were not marked, and the number of follicles was reduced in the presence of metastases in the omentum. The analysis of CD34+ cells distribution showed that they were identified both in the tumor and in the areas of the omentum adjacent to the tumor, which indicates a pronounced angiogenesis. An irregular distribution of CD7+ and CD8+ and CD4+ cells was revealed in the tumor tissues, as well as in the surroundings. Simultaneously with the expression of P53 protein, ki67 protein expression is revealed in the significant number of tumor cells (including endothelial cells of tumor blood vessels). The proportion of ki67+ cells in the tumor cell population was 60.1±3.3%. The presence of a large number of ki67+cells in the presence of P53 protein expression in them indicates the aggressiveness of the tumor, as well as a disturbance of apoptosis regulatory mechanisms in the cells. Ki67 expression was low in the omentum areas unaffected by metastases, and it was revealed in the certain areas of connective tissue in fibroblastic programmed differentiation cells. Conclusion. The results obtained indicate significant plasticity and reactivity of great omentum in the presence of tumor process in the body and confirm the important role of great omentum in protective reactions.

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FACTORS INFLUENCING THE INTERMITTENT PASSAGE OF LOCKE'S SOLUTION INTO LIVING SKIN

Journal of Experimental Medicine ◽

10.1084/jem.73.1.85 ◽

1941 ◽

Vol 73 (1) ◽

pp. 85-108 ◽

Cited By ~ 9

Author(s):

Philip D. McMaster

Keyword(s):

Connective Tissue ◽

Blood Vessels ◽

Atmospheric Pressure ◽

Interstitial Fluid ◽

Lymphatic Vessels ◽

Intermittent Flow ◽

Venous Obstruction ◽

Factors Influencing ◽

Interstitial Connective Tissue ◽

Living Mouse

Minute amounts of Locke's or Tyrode's solution have been brought into contact with the interstitial connective tissue of the skin of the living mouse, at atmospheric pressure, in such a manner that the blood or lymphatic vessels are not entered directly. Under such circumstances these absorbable fluids enter the tissue spontaneously. Entrance is strikingly intermittent, not continuous, and so too when very slight pressures are brought to bear on the fluids (1). Hyperemia of the tissues, with accompanying dilatation of the blood vessels, increases the entrance of fluids at atmospheric pressure but it is still intermittent. By contrast, venous obstruction leads to intermittent backflow into the apparatus, but reflex hyperemia, following release of the obstruction, is attended by an increase of flow into the tissues in spite of the great reactive dilatation of vessels. The inflow is also intermittent. If the skin is deprived of circulation, fluid does not enter it at all at atmospheric pressure, though it moves in regularly and continuously if slight pressure is put upon it. Edema-forming fluids, described in the text, also enter in a continuous manner when forced into the skin of either living or dead animals. So too do serum and sperm oil. The findings indicate that the passage of interstitial fluid into the blood vessels may be intermittent under normal circumstances and its escape from them as well. The observed occurrence of intermittent flow in the blood vessels of several tissues (9, 15–25) will go far to account for the intermittent entrance of fluid into the skin.

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LOCALIZATION OF α-FETOPROTEIN IN HUMAN AND RAT FETAL LIVERS AN IMMUNOHISTOCHEMICAL METHOD USING HORSERADISH PEROXIDASE

Journal of Histochemistry & Cytochemistry ◽

10.1177/22.6.414 ◽

1974 ◽

Vol 22 (6) ◽

pp. 414-418 ◽

Cited By ~ 3

Author(s):

N. C. NAYAK ◽

P. K. DAS ◽

U. N. BHUYAN ◽

ASHA MITTAL

Keyword(s):

Connective Tissue ◽

Horseradish Peroxidase ◽

Immunohistochemical Method ◽

Immunoperoxidase Technique ◽

Serial Sections ◽

Immunofluorescence Method ◽

Immunohistochemical Technique

α-Fetoprotein (AFP) was successfully demonstrated in paraffin-embedded sections of human and rat fetal livers, by a multilayering immunohistochemical technique using the peroxidase-antiperoxidase system. Serial sections simultaneously subjected to immunofluorescence showed identical sites of localization in hepatocytic cytoplasm and sometimes in perivenous connective tissue. AFP-containing hepatocytes were located around efferent veins as well as randomly in the lobule. The immunoperoxidase technique has certain advantages over the immunofluorescence method which would justify the former's application in studies on the dynamics of AFP synthesis.

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On the Pituitary of the Perch (Peroa Fluviatilis)

Journal of Cell Science ◽

10.1242/jcs.s2-83.331.299 ◽

1942 ◽

Vol s2-83 (331) ◽

pp. 299-316

Author(s):

T. KERR

Keyword(s):

Connective Tissue ◽

Blood Vessels ◽

Perca Fluviatilis ◽

Cell Types ◽

Anterior Lobe ◽

Intermediate Lobe ◽

General Description ◽

Perch Perca Fluviatilis ◽

Higher Types ◽

Different Cell Types

1. A general description is given of the pituitary of the perch (Perca fluviatilis L.), and histological details of its various parts. The subdivisions of the glandular component are confluent with each other but distinguished by their different cell types. The nervous lobe makes contact with all three of the subdivisions, but is separated from them by a layer of connective tissue, incomplete in particular areas. 2. The anterior glandular region (anterior lobe) has an anterior chromophil and a posterior chromophobe zone. The middle glandular region (transitional lobe) possesses brightly staining acidophils and basophils as well as chromophobes. The acidophils form a dorsal sheet, deeply indented by processes of the nervous lobe, the basophils lie ventrally and posteriorly, and chromophobes are common towards the extremities of the indentations. The posterior glandular region (intermediate lobe) is elaborately penetrated by nervous lobe processes; the cells are small and consist of amphiphils, dull basophils, and occasional dull acidophils. The possible homologies of these regions to the lobes of higher types are discussed. The nervous lobe is of loose glial tissue with many nuclei and blood vessels and some reticular and collagenous fibres. 3. Strongly acidophil spheres of various sizes and in various numbers occur in the middle glandular region. They originate in ‘sphere cells’ resembling eosinophil leucocytes and after enlarging become free in the tissues of the region. Later they appear to pass into the posterior processes of the nervous lobe to be the larger bodies of the Herring material. Finally these larger elements appear to break down to form a fine granulation, whose further fate could not be followed.

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Light and Scanning Electron Microscopy of Human Spinal Ligaments

MRS Proceedings ◽

10.1557/proc-55-333 ◽

1985 ◽

Vol 55 ◽

Cited By ~ 1

Author(s):

L.-H. Yahia ◽

G. Drouin ◽

C.-H. Rivard

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Connective Tissue ◽

Idiopathic Scoliosis ◽

Blood Vessels ◽

Structural Changes ◽

Loose Connective Tissue ◽

Morphological Studies ◽

Spinal Ligaments ◽

Scanning Electron

ABSTRACTSpinal ligaments were obtained from normal and scoliotic individuals. Detailed morphological studies were carried out on the yellow, interspinous and supraspinous ligaments by light and scanning electron microscopy. Normal yellow ligaments are mostly constituted of dense elastin fibers with only a few collagen fibers and sparse blood vessels, while in normal interspinous and supraspinous ligaments, the presence of collagen is highly dominant. In the latter structures, the collagen fascicles are characterized by a regular waviness morphology. The fibrils constituting the fascicles appear either parallel or helical with respect to the fascicle axis. Structural changes are observed in the spinal ligaments of patients with congenital as well as idiopathic scoliosis. For yellow ligaments, only slight differences are found between normal and scoliotic specimens. However, alterations in collagen waviness and architecture are observed mainly in the supraspinous ligaments and to a lesser extent in the interspinous ligaments. In addition, increases in the cellularity, loose connective tissue and vessels are found in both forgoing ligaments. These results indicate that the more pronounced scoliosis-related changes occur in ligaments having the farthest distance from the axis of flexion-rotation.

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Connective tissue growth factor (CCN2) in blood vessels

Vascular Pharmacology ◽

10.1016/j.vph.2013.01.004 ◽

2013 ◽

Vol 58 (3) ◽

pp. 189-193 ◽

Cited By ~ 25

Author(s):

Markella Ponticos

Keyword(s):

Growth Factor ◽

Connective Tissue ◽

Blood Vessels ◽

Connective Tissue Growth Factor ◽

Tissue Growth

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The Arterial Anatomy of the Talus

Foot & Ankle ◽

10.1177/107110078300400204 ◽

1983 ◽

Vol 4 (2) ◽

pp. 64-72 ◽

Cited By ~ 107

Author(s):

Richard H. Gelberman ◽

Wayne W. Mortensen

Keyword(s):

Avascular Necrosis ◽

Blood Supply ◽

The Body ◽

The Other ◽

Small Areas ◽

Sinus Tarsi ◽

Fresh Cadaver ◽

Tarsal Canal ◽

Arterial Anatomy

The extraosseous and intraosseous vascularity of the talus was studied in 26 fresh cadaver limbs. The specimens were injected with latex or Batson's compound, debrided by a nondissection technique, and cleared by a modified Spalteholz method. The extraosseous vascularity was through the branches of the three major regional arteries which entered the five nonarticulating surfaces of the bone. The major blood supply to the body was provided by the artery of the tarsal canal. The deltoid and sinus tarsi vessels provided significant minor sources of vascularity. The superior neck and posterior tubercle vessels supplied small areas of the body, but did have anastomoses with the other arteries in some specimens. These vascular patterns correlated well with the reported incidence of avascular necrosis of the body of the talus following injury.

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Vasculitis

10.1093/med/9780199568741.003.0272 ◽

2018 ◽

Author(s):

Raashid Luqmani

Keyword(s):

Connective Tissue ◽

Blood Vessel ◽

Blood Vessels ◽

Vessel Wall ◽

Connective Tissue Diseases ◽

Blood Vessel Wall ◽

Target Organs ◽

Laboratory Features ◽

Life Threatening ◽

Pulmonary Capillaritis

The vasculitides are a heterogeneous group of disorders that can range from mild inflammation of blood vessels in the skin, to organ- and life-threatening diseases. The term ‘vasculitis’ is a pathological description of blood vessel wall inflammation which leads to ischaemia and infarction of the target organs. Definitions and classifications of the primary vasculitides are mainly based on the predominant calibre of the blood vessels involved but incorporate clinical, pathological, and laboratory features. The secondary vasculitides usually occur in the context of other connective tissue diseases and are not discussed further in this section. Goodpasture’s disease is not usually included in the primary vasculitides, but has compatible clinical features of pulmonary capillaritis and glomerulonephritis.

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