Serum Haptoglobin Levels in Ocular Behçet Disease and Acute Phase Proteins in the Course of Behçet Disease

ABSTRACT: The aim of this study was to evaluate and to compare the possible inflammatory changes by screening acute phase proteins concentrations in healthy bitches subjected to ovariohysterectomy. Minimally invasive and conventional (laparotomy) ovariohysterectomies were performed in 17 client-owned adult female mixed breed dogs. Nine animals were subjected to minimally invasive and eight animals to conventional ovariohysterectomy. Blood samples were taken before surgery, 24, 48 hours, and seven days postoperatively. Serum C-reactive concentration was determined by a commercial ELISA kit and serum haptoglobin concentration was measured via hemoglobin binding assay, both previously validated for use in dogs. As the data did not meet the normal distribution criteria, the nonparametric Kruskall-Wallis was performed to compare quantitative variables between groups. One-way ANOVA and the Friedman test were used for multiple comparisons between time points, with a P<0.05 considered significant. C-reactive protein concentration was significantly different (P<0.0001) at 24 hours postoperatively between groups. There was no significant difference in haptoglobin concentration between groups. C-reactive protein and haptoglobin concentrations were significantly different at 24 and 48 hours postoperatively for minimally invasive and conventional ovariohisterectomies. These findings provided an overview of the short-term inflammatory effects produced by minimally invasive and conventional ovariohysterectomies.

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Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses

Animals ◽

10.3390/ani11102952 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2952

Author(s):

Maciej Przewoźny ◽

Magdalena Senderska-Płonowska ◽

Anna Rząsa ◽

Heliodor Wierzbicki ◽

Jacek Borkowski ◽

...

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Proteinase Inhibitors ◽

Maximum Level ◽

Study Groups ◽

Orthopedic Procedures ◽

Control Group ◽

Complicated Case ◽

Bone Removal ◽

Serum Haptoglobin

Background: Arthroscopy and splint bone removal are the common orthopedic procedures in horses. Estimation of the dynamics of acute phase proteins in postoperative monitoring seems to be interesting diagnostic approach. The aim of the study was to investigate changes in the concentrations of plasma inflammatory markers—fibrinogen, haptoglobin, and protease inhibitors—following orthopedic surgery in horses. The study involved 114 horses, divided into two study groups undergoing: arthroscopy (41 horses) and splint bone removal (13 horses). The control group consisted of 60 healthy horses. The blood was collected before the surgery and 24, 48, 72 h, 5, 7, 10, 14 and 28 days after the surgery. Plasma fibrinogen, serum haptoglobin and proteinase inhibitors were measured. Results: In non-complicated cases of arthroscopy and splint bone removal, fibrinogen and haptoglobin increased stepwise from 24 h, achieved the maximum level at 72 h and returned to preoperative levels after 10–14 days. In one complicated case after arthroscopy surgery the marked increase in fibrinogen and haptoglobin concentrations was observed 24 h earlier than standard parameters of inflammation Conclusion: The study shows the evolution of APPs after arthroscopy and splint bone removal in 28 days postsurgery period and in the case of one complicated case of arthroscopy.

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Comparison of serum procalcitonin, haptoglobin and C-reactive protein levels in goats with Corynebacterium pseudotuberculosis

Indian Journal of Animal Research ◽

10.18805/ijar.b-932 ◽

2018 ◽

Author(s):

G. Akgul ◽

M.B. Akgul ◽

D. Ozen ◽

S. Kahya Demýrbýlek

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Dairy Farm ◽

Diagnostic Information ◽

Control Group ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein ◽

Serum Haptoglobin ◽

Significant Difference

This study was conducted to investigate the response of acute phase proteins, mainly Haptoglobin, C-reactive protein, Procalcitonin and the negative acute phase response, especially albumin in goats suffering from C. pseudotuberculosis. A total of 19 Turkish hair goats between the ages of 1.5 and 2 years in a special dairy farm was selected for the study, consisting of 9 healthy animals and 10 with C. pseudotuberculosis. There was a statistically significant difference in serum haptoglobin, C-reactive protein, procalcitonin and albumin levels in goats with C. pseudotuberculosis, compared to the control group (p less than 0.05). The results showed that Haptoglobin, C-reactive protein and Procalcitonin produce a higher, and Alb a lower, response in goats with C. pseudotuberculosis compared to the control. The presented study suggests that C. pseudotuberculosis can influence the level of acute phase proteins in goats. These results indicate that monitoring a number of acute phase proteins can increase the diagnostic information available for this disease.

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Serum concentrations of some acute phase proteins in cats with anaemia

Medycyna Weterynaryjna ◽

10.21521/mw.5998 ◽

2018 ◽

Vol 74 (1) ◽

pp. 5998-2018

Author(s):

GÜLTEN EMEK TUNA ◽

CEREN DINLER ◽

GAMZE SEVRI EKREN AŞICI ◽

BÜLENT ULUTAŞ

Keyword(s):

Haemolytic Anaemia ◽

Acute Phase ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Serum Amyloid ◽

Serum Concentrations ◽

Healthy Group ◽

Acid Glycoprotein ◽

Amyloid A ◽

Serum Haptoglobin

Serum concentrations of acute phase proteins can provide valuable diagnostic information in the detection and monitoring of disease. The available information on the acute phase response in cats with anaemia is limited. The aim of this study was to retrospectively evaluate serum concentrations of haptoglobin, serum amyloid A, α1 acid glycoprotein and their clinical importance in cats with anaemia. Thirty-four anaemic cats and ten healthy cats were enrolled this study. After individual diagnoses had been established, the cats were divided into three groups (healthy group, haemolytic group and non-haemolytic group). Serum acute phase protein concentrations were analysed using specific commercially available test kits in an ELISA reader device. Serum amyloid A and serum α1 acid glycoprotein concentrations were significantly higher in the anaemic groups compared with the healthy group. Haptoglobin concentrations were significantly higher in cats from the non-haemolytic anaemia group than they were in healthy animals and those from the haemolytic anaemia group. Although serum haptoglobin concentrations were lower than in the healthy group, there was no significant difference between the haemolytic anaemia group and the healthy group. The results of this study suggest that serum amyloid A and α1 acid glycoprotein could be useful in the diagnosis and determination of inflammation in cats with anaemia. Serum haptoglobin depletion may be used for diagnosis of haemolysis in cats with haemolytic anaemia. In addition, this study has contributed to the limited data available on acute phase protein concentrations in cats with anaemia..

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Influence of the adrenal glucocorticoids on the stimulation of synthesis of hepatic ribonucleic acid and plasma acute-phase globulins by leucocytic endogenous mediator

Biochemical Journal ◽

10.1042/bj1560025 ◽

1976 ◽

Vol 156 (1) ◽

pp. 25-32 ◽

Cited By ~ 36

Author(s):

W L Thompson ◽

F B Abeles ◽

F A Beall ◽

R E Dinterman ◽

R W Wannemacher

Keyword(s):

Acute Phase ◽

Rna Synthesis ◽

Acute Phase Proteins ◽

Acute Phase Protein ◽

Liver Perfusion ◽

Subcutaneous Injections ◽

Serum Haptoglobin ◽

Increase Rate ◽

Stimulation Of

An injection of unpurified leucocytice endogenous mediator into rats results in an increased incorporation of [6(-14)C]orotate into hepatic RNA, an increase in the concentration of RNA associated with the bound ribosomal fraction of liver, and increases in the concentrations in serum of acute-phase proteins such as alpha2-macrofoetoprotein and haptoglobin. If given 3 days after adrenalectomy or 7 days after hypophysectomy, leucocyte factor did not induce the increase in RNA synthesis or alpha2-macrofoetoprotein concentrations but did stimulate an increase in serum haptoglobin. When hypophysectomized or adrenalectomized rats received daily subcutaneous injections of 0.5mg of cortisol, leucocyte factor again induced a significant increase in the synthesis of hepatic RNA and an increase in the concentration of serum alpha2-macrofoetoprotein. These observations suggest that leucocyte factor can regulate acute-phase-protein synthesis at several different sites, one or more of which requires permissive action of the glucocorticoid hormones. Futher, leucocyte factor will stimulate an increase rate of incorporation of orotate into hepatic ribosomes when added in vitro in the presence of cortisol to a liver-perfusion system. Thus the stimulatory effect of leucocyte factor may be directy on liver but may require the presence of other hormones to stimulate the incorporation of orotate into RNA.

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