Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses

Background: Arthroscopy and splint bone removal are the common orthopedic procedures in horses. Estimation of the dynamics of acute phase proteins in postoperative monitoring seems to be interesting diagnostic approach. The aim of the study was to investigate changes in the concentrations of plasma inflammatory markers—fibrinogen, haptoglobin, and protease inhibitors—following orthopedic surgery in horses. The study involved 114 horses, divided into two study groups undergoing: arthroscopy (41 horses) and splint bone removal (13 horses). The control group consisted of 60 healthy horses. The blood was collected before the surgery and 24, 48, 72 h, 5, 7, 10, 14 and 28 days after the surgery. Plasma fibrinogen, serum haptoglobin and proteinase inhibitors were measured. Results: In non-complicated cases of arthroscopy and splint bone removal, fibrinogen and haptoglobin increased stepwise from 24 h, achieved the maximum level at 72 h and returned to preoperative levels after 10–14 days. In one complicated case after arthroscopy surgery the marked increase in fibrinogen and haptoglobin concentrations was observed 24 h earlier than standard parameters of inflammation Conclusion: The study shows the evolution of APPs after arthroscopy and splint bone removal in 28 days postsurgery period and in the case of one complicated case of arthroscopy.

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Comparison of serum procalcitonin, haptoglobin and C-reactive protein levels in goats with Corynebacterium pseudotuberculosis

Indian Journal of Animal Research ◽

10.18805/ijar.b-932 ◽

2018 ◽

Author(s):

G. Akgul ◽

M.B. Akgul ◽

D. Ozen ◽

S. Kahya Demýrbýlek

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Dairy Farm ◽

Diagnostic Information ◽

Control Group ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein ◽

Serum Haptoglobin ◽

Significant Difference

This study was conducted to investigate the response of acute phase proteins, mainly Haptoglobin, C-reactive protein, Procalcitonin and the negative acute phase response, especially albumin in goats suffering from C. pseudotuberculosis. A total of 19 Turkish hair goats between the ages of 1.5 and 2 years in a special dairy farm was selected for the study, consisting of 9 healthy animals and 10 with C. pseudotuberculosis. There was a statistically significant difference in serum haptoglobin, C-reactive protein, procalcitonin and albumin levels in goats with C. pseudotuberculosis, compared to the control group (p less than 0.05). The results showed that Haptoglobin, C-reactive protein and Procalcitonin produce a higher, and Alb a lower, response in goats with C. pseudotuberculosis compared to the control. The presented study suggests that C. pseudotuberculosis can influence the level of acute phase proteins in goats. These results indicate that monitoring a number of acute phase proteins can increase the diagnostic information available for this disease.

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Establishment of peritoneal liquid electrophoretogram from healthy horses and horses submitted to experimentally induced intestinal obstruction

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-41625993 ◽

2014 ◽

Vol 66 (3) ◽

pp. 665-671 ◽

Cited By ~ 1

Author(s):

A.F.S. Nogueira ◽

P.A. Di Filippo ◽

L.A. Anai ◽

M.C. Vieira ◽

K.M.M.G. Simplício ◽

...

Keyword(s):

Intestinal Obstruction ◽

Acute Phase ◽

Acute Phase Proteins ◽

Immunoglobulin A ◽

Control Group ◽

Protein Levels ◽

Sds Page ◽

Significant Difference ◽

Page Electrophoresis ◽

Experimentally Induced

The initial inflammatory stages of the colic syndrome include changes known as acute phase response. The aim of this study was to contribute with the establishment of reference values concerning the electrophoretogram of peritoneal liquid from healthy horses and horses submitted to experimentally induced intestinal obstruction. Twenty-one horses were allotted in four groups: duodenal obstruction (DG), ileum obstruction (IG), left-dorsal colon obstruction (MG), and control group (CG). Peritoneal liquid was sampled before obtruction (T0), with 3 hours of obstruction (T3) and 6, 30, 102 and 174 hours after desobstructing (T6, T30, T102 and T174, respectively). Total protein levels were determined by the biuret method and protein fractions were obtained by SDS-PAGE electrophoresis. The acute phase proteins (APP) identified were Immunoglobulin-A, ceruloplasmin, transferrin, albumin, α1-antitrypsin, heavy and light chains of immunoglobulin-G, haptoglobin, α1-acid glycoprotein and a still unnamed protein, which was called P24. There was no difference (P>0.3) in protein levels among groups, although a significant difference (P>0.05) was observed between distinct experimental moments in each group evidencing a higher response of the APP in the obstructed groups. The APP fractioning of the peritoneal liquid was standardized to establish a standard curve for healthy equines and those submitted to induced intestinal obstruction. Moreover, it was verified that the SDS-PAGE electrophoresis was sensitive and effective to help diagnose abdominal inflammatory processes.

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GENE POLYMORPHISM ANALYSIS OF CHEMOKINES, CHEMOKINE RECEPTORS, ACUTE PHASE PROTEINS, AND CD14 IN FEMALE OBESITY DEVELOPMENT

Medical Immunology (Russia) ◽

10.15789/1563-0625-2018-5-739-746 ◽

2018 ◽

Vol 20 (5) ◽

pp. 739-746

Author(s):

O. V. Kochetova ◽

L. Z. Akhmadishina ◽

G. F. Korytina ◽

Т. V. Victorova

Keyword(s):

Body Weight ◽

Glucose Level ◽

Acute Phase ◽

Chemokine Receptors ◽

Acute Phase Proteins ◽

Fasting Glucose ◽

Polymorphic Locus ◽

Control Group ◽

Polymorphism Analysis ◽

Fasting Glucose Level

In the present study, we have investigated frequency of genotypes and functional alleles of genes encoding chemokines (CXCL12 rs1801157, CCL2 rs1024611), chemokine receptors (CCR5 del32, CX3CR1 rs3732378), acute phase proteins SAA rs1136743, and CD14 rs2569190 polymorphisms among Tatar obese or overweight women from the Republic of Bashkortostan.The group of patients comprised unrelated women with obesity (BMI ≥ 30 kg/m2, n = 225), females with overweight (BMI 25.0-29.9 kg/m2, n = 184), and control group of women (n = 327) BMI < 25.0 kg/m2. Genotyping was performed by PCR-RFLP analysis. Patients and controls differed in such parameters as body weight (p = 0.00001), BMI level (p = 0.001) and fasting glucose level (p = 0.0001).An association was revealed between obesity andAG-AAgenotypes (p = 0.007) andAallele (p = 0.003) of polymorphic locus rs3732378 ofCXCR1gene, as well asTTgenotype (p = 0.027) andTallele (p = 0.021) of polymorphic locus rs1136743 ofSAAgene. It has been shown that theAAgenotype of polymorphic locus rs3732378 of theCX3CR1gene is associated with increased body weight (p = 0.002) and elevated BMI (p = 0.018); theGGgenotype of polymorphic locus rs1024611 of theCCL2gene is associated with elevated fasting glucose level (p = 0.001).As based on clinical and genetic data and using logistic regression, some statistically significant differences were revealed, which allow to predict development of obesity in Tatar women.

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Acute phase proteins in bitches subjected to conventional and minimally invasive ovariohysterectomy

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5132 ◽

2018 ◽

Vol 38 (11) ◽

pp. 2124-2128 ◽

Cited By ~ 1

Author(s):

Elizabeth M.S. Schmidt ◽

Camila P. Rubio ◽

Funmilola Thomas ◽

João C.P. Ferreira ◽

David P. Eckersall

Keyword(s):

Minimally Invasive ◽

Acute Phase ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Reactive Protein ◽

Serum Haptoglobin ◽

Hemoglobin Binding ◽

Mixed Breed ◽

Significant Difference ◽

Haptoglobin Concentration

ABSTRACT: The aim of this study was to evaluate and to compare the possible inflammatory changes by screening acute phase proteins concentrations in healthy bitches subjected to ovariohysterectomy. Minimally invasive and conventional (laparotomy) ovariohysterectomies were performed in 17 client-owned adult female mixed breed dogs. Nine animals were subjected to minimally invasive and eight animals to conventional ovariohysterectomy. Blood samples were taken before surgery, 24, 48 hours, and seven days postoperatively. Serum C-reactive concentration was determined by a commercial ELISA kit and serum haptoglobin concentration was measured via hemoglobin binding assay, both previously validated for use in dogs. As the data did not meet the normal distribution criteria, the nonparametric Kruskall-Wallis was performed to compare quantitative variables between groups. One-way ANOVA and the Friedman test were used for multiple comparisons between time points, with a P<0.05 considered significant. C-reactive protein concentration was significantly different (P<0.0001) at 24 hours postoperatively between groups. There was no significant difference in haptoglobin concentration between groups. C-reactive protein and haptoglobin concentrations were significantly different at 24 and 48 hours postoperatively for minimally invasive and conventional ovariohisterectomies. These findings provided an overview of the short-term inflammatory effects produced by minimally invasive and conventional ovariohysterectomies.

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Assessment of Acute-Phase Protein Response Associated with the Different Pathological Forms of Bovine Paratuberculosis

Animals ◽

10.3390/ani10101925 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1925

Author(s):

José Espinosa ◽

Rubén de la Morena ◽

Julio Benavides ◽

Carlos García-Pariente ◽

Miguel Fernández ◽

...

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Bacterial Load ◽

Control Group ◽

Serum Samples ◽

Focal Lesions ◽

Amyloid A ◽

Cell Mediated Immune Response ◽

Protein Response ◽

Antibody Levels

In this study, the concentrations of two acute-phase proteins (APPs), haptoglobin (Hp) and serum amyloid A (SAA), were quantitatively assessed in serum samples from cattle naturally infected with paratuberculosis (PTB). APP profiles were compared across 190 animals classified according to the different pathological forms associated with infection: uninfected (n = 59), with focal lesions (n = 73), multifocal lesions (n = 19), and diffuse paucibacillary (n = 11) and diffuse multibacillary lesions (n = 28). Our results showed a significant increase in both APPs in infected animals compared to the control group, with differences depending on the type of lesion. Hp and SAA levels were increased significantly in all infected animals, except in cows with diffuse multibacillary lesions that showed similar values to non-infected animals. The expression pattern of both APPs was similar and negatively correlated with the antibody levels against PTB. These results indicate that the release of Hp and SAA is related to the presence of PTB lesions associated with a high cell-mediated immune response and a lower bacterial load, suggesting that the pro-inflammatory cytokines that are associated with these forms are the main stimulus for their synthesis. These molecules could show some potential to be used as putative biomarkers of PTB infection, particularly for the identification of subclinical animals showing pathological forms related to latency or resistance to the development of advanced lesions.

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PROCALCITONIN, C-REACTIVE PROTEIN AND FIBRINOGEN LEVELS IN CASES WITH MECHANIC BOWEL OBSTRUCTION AND THEIR CHANGE IN RELATION TO THE DURATION OF OBSTRUCTION: AN EXPERIMENTAL STUDY

International Surgery ◽

10.9738/intsurg-d-15-00122.1 ◽

2017 ◽

Author(s):

Hakan Guzel ◽

Sahin Kahramanca ◽

Oskay Kaya ◽

Gulay Ozgehan ◽

Demet Yilmazer ◽

...

Keyword(s):

Acute Phase ◽

Bowel Obstruction ◽

Surgical Intervention ◽

Late Phase ◽

Study Groups ◽

Control Group ◽

C Reactive Protein ◽

Acute Phase Reactants ◽

Reactive Protein ◽

Mechanical Bowel Obstruction

The need and timing of surgical intervention in patients with adhesive bowel obstruction is a dilemma. We aimed to investigate the role of three acute-phase reactants, namely procalcitonin (PCT), fibrinogen and C-reactive protein (CRP) in this clinical condition We chose a rat model whose mechanical bowel obstruction was created with caecum ligation. There were two study groups and one control group. Each group contained ten subjects. The study groups had and six-hour obstruction samples. Blood PCT, fibrinogen and CRP levels were measured before and after the surgical procedure. These parameters were compared between the groups and they were also evaluated with the degree of histopathological changes occurred in terminal ileal tissue samples. Compared with the control group, PCT measurements showed a mild decrease in the early phase of obstruction but a significant elevation in the late phase (p: 0,977 and p: 0,001). Unlike PCT, fibrinogen levels increased at first but decreased later (p: 0,978 and p: 0,326). We observed an increase in CRP levels parallel to the prolonged duration of obstruction (p: 0,987 and p: 0,134). With regard to mucosal injury, PCT levels increased at first, and then decreased (p: 0,003). On the other hand, fibrinogen and CRP levels decreased at first, and then increased (p: 0,139 and p: 0,102). The acute-phase reactants PCT, fibrinogen and CRP associated with the duration of obstruction may help to determine the time of surgical intervention in patients with adhesive mechanical bowel obstruction.

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Serum protein concentrations, including acute phase proteins, in calves with hepatogenous photosensitization

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352007000600001 ◽

2007 ◽

Vol 59 (6) ◽

pp. 1355-1358 ◽

Cited By ~ 7

Author(s):

J.J. Fagliari ◽

M. Passipieri ◽

H.T. Okuda ◽

S.L. Silva ◽

P.C. Silva

Keyword(s):

Serum Protein ◽

Acute Phase ◽

Serum Proteins ◽

Acute Phase Proteins ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Clinical Signs ◽

Control Group ◽

Molecular Weights ◽

Therapeutic Procedures

One hundred 6- to 12-month-old Nelore calves were allotted into control group (G1; 50 healthy calves) and photosensitization group (G2; n= 50). Blood samples were collected 12 to 24 hours after the onset of dermatitis (M1), and 15 to 30 days after that (M2), at time of resolution of clinical signs. Serum protein electrophoresis was performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Eighteen serum proteins with molecular weights ranging from 16,000 to 189,000 daltons (Da) were identified in all calves. In M1 and M2 serum concentrations of proteins with molecular weights of 115,000Da (ceruloplasmin), 61,000Da (a1-antitrypsin), 45,000Da (haptoglobin), and 40,000Da (acid glycoprotein) were significantly increased in calves. In conclusion, measurement of serum acute phase protein concentrations may be useful in monitoring the progression of bovine hepatogenous photosensitization, including guide probable alteration on therapeutic procedures.

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Serum Haptoglobin Levels in Ocular Behçet Disease and Acute Phase Proteins in the Course of Behçet Disease

European Journal of Ophthalmology ◽

10.1177/112067210801800520 ◽

2008 ◽

Vol 18 (5) ◽

pp. 787-791 ◽

Cited By ~ 4

Author(s):

F.N. Yalçindağ ◽

A. YalçIndağ ◽

O. Çağlayan ◽

Ö. Özdemir

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Behçet Disease ◽

Behcet Disease ◽

Serum Haptoglobin

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THE ANALYSIS OF METHYLATION OF DNA PROMOTER OF SFRP2 GENE IN PATIENTS WITH HYPERPLASTIC PROCESSES OF THE ENDOMETRIUM

Experimental Oncology ◽

10.31768/2312-8852.2018.40(2):109-113 ◽

2018 ◽

Vol 40 (2) ◽

pp. 109-113

Author(s):

V G Marichereda ◽

N A Bykovа ◽

V V Bubnov ◽

G S Manasova ◽

T Y Moskalenko ◽

...

Keyword(s):

Gene Promoter ◽

Maximum Level ◽

Intraepithelial Neoplasia ◽

Study Groups ◽

Control Group ◽

Healthy Women ◽

Methylation Of Dna ◽

Specific Pcr ◽

Endometrial Intraepithelial Neoplasia ◽

Specific Pcr Assay

The hyperplastic processes of the endometrium can arise not only against the background of excessive influence of estrogen, but also against the background of epigenetic damages that affect apoptosis, cell proliferation, differentiation, and adhesion, and DNA reparation. The aim of our study was to investigate and analyze the status of methylation of the promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium. Materials and Methods: The study groups were the following: I — patients with endometrial hyperplasia (EH, n = 9); II — patients with endometrial intraepithelial neoplasia (EIN, n = 10), III — control groups: 1) with endometrial cancer (EC, n = 4), and 2) healthy women (n = 4). Determination of promoter methylation of SFRP2 gene was carried out by the semiquantitative method of methylation-specific PCR assay. Results: The maximum level of methylation of SFRP2 gene promoter had been revealed in patients with EC — 42.80 ± 3.55% (р < 0.05). The patients of the I group had the lowest values of methylation of SFRP2 gene promoter — 10.66 ± 0.85%, while in patients of the II group this indicator was higher — 20.60 ± 0.95% (р < 0.05). In healthy women of the control group, methylation of SFRP2 gene promoter was detected in none of the samples. Conclusion: The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20–25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients.

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The role of alpha-2-macroglobulin in pathogenesis of rheumatoid arthritis and system lupus erythematosus

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2010-5-39-44 ◽

2010 ◽

Vol 9 (5) ◽

pp. 39-44

Author(s):

V. N. Zorina ◽

I. G. Kozlov ◽

R. M. Zorina ◽

N. A. Trofimenko ◽

T. S. Chirikova ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Disease Progression ◽

Acute Phase ◽

Lupus Erythematosus ◽

Acute Phase Proteins ◽

Serum Levels ◽

Control Group ◽

Healthy Donors ◽

Significant Difference

We investigated serum levels of alpha-2-macroglobulin (α2—MG) and some of its complexes, namely α2-MG-plasmin (α2-MG— Pl) and α2-MG—IgG at a rheumatoid arthritis (RA) 2—3 degrees of activity (65 patients), a system lupus erythematosus (SLE) of 2-3 degrees of activity (30 patients) and 55 healthy donors as a control group. It is shown, that at SLE the total level of α2-MG is invariable, and at RA — decreases significantly in comparison with the healthy. The concentration of complexes was raised at pathology, but at RA this rising was expressed much more strongly, than at SLE. At studying of correlations of levels of α2-MG, α2-MG—Pl and α2-MG— IgG among themselves and with some several cytokines and acute phase proteins, it is shown, that there is some significant difference between normal and pathological correlative relations and allows us to suspect that at SLE, the α2-MG and its complexes participate in a pathogenesis, and at RA α2-MG becomes the major immunogenesis factor and the significant reason of disease progression.

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