Characterization of Bacteria and Fungi Bioaerosol in the Indoor Air of selected Primary Schools in Malaysia

2011 ◽  
Vol 20 (6) ◽  
pp. 607-617 ◽  
Author(s):  
Nor Husna Mat Hussin ◽  
Lye Munn Sann ◽  
Mariana Nor Shamsudin ◽  
Zailina Hashim

This study reports the types and concentrations of bacterial and fungal bioaerosols found in five randomly selected primary schools in Malaysia. Normal flora bacteria was the most frequently isolated bacteria including Staphylococcus spp., Pseudomonas spp. and Bacillus spp. Terribacillus spp. found in this study had never been reported before. The most frequently isolated fungal genera were Aspergillus, Penicillium, Fusarium, Rhizopus and Zygomycetes. The average concentration of bacteria in indoor and outdoor air were 1025 ± 612 CFU/m3 and 1473 ± 1261 CFU/m3, respectively, while the average concentration of fungal bioaerosol in indoor and outdoor air were 292 ± 83 CFU/m3 and 401 ± 235 CFU/m3, respectively. The percentages of bacterial and fungal samples that were within the American Conference of Industrial Hygenists (ACGIH) recommended levels were 44% and 33.8%, respectively. The ratio of indoor to outdoor fungi concentration was below 1.0, suggesting minimal indoor generative source for fungal bioaerosols. However, the ratio of indoor to outdoor bacteria concentration was approaching 1.0, suggesting the presence of potential internal generative source and inadequate ventilation. Building occupants might be one of the potential sources of bacteria in the indoor air as the bacteria concentrations without occupants were significantly lower than with occupants (p < 0.05).

2014 ◽  
Vol 2014 ◽  
pp. 1-3 ◽  
Author(s):  
Normah Awang ◽  
Farhana Jamaluddin

This study was carried out to determine the concentration of lead (Pb), anions, and cations at six primary schools located around Kuala Lumpur. Low volume sampler (MiniVolPM10) was used to collect the suspended particulates in indoor and outdoor air. Results showed that the concentration of Pb in indoor air was in the range of 5.18 ± 1.08 μg/g–7.01 ± 0.08 μg/g. All the concentrations of Pb in indoor air were higher than in outdoor air at all sampling stations. The concentrations of cations and anions were higher in outdoor air than in indoor air. The concentration ofCa2+(39.51 ± 5.01 mg/g–65.13 ± 9.42 mg/g) was the highest because the cation existed naturally in soil dusts, while the concentrations ofNO3-andSO42-were higher in outdoor air because there were more sources of exposure for anions in outdoor air, such as highly congested traffic and motor vehicles emissions. In comparison, the concentration ofNO3-(29.72 ± 0.31 μg/g–32.00 ± 0.75 μg/g) was slightly higher thanSO42-. The concentrations of most of the parameters in this study, such asMg2+,Ca2+,NO3-,SO42-, andPb2+, were higher in outdoor air than in indoor air at all sampling stations.


2021 ◽  
Vol 16 (1) ◽  
pp. 34-45
Author(s):  
Nur Baitul Izati Rasli ◽  
Nor Azam Ramli ◽  
Mohd Rodzi Ismail

Occupants of buildings spent most of their time indoors and posed themselves to the indoor biological contaminants (i.e. bacteria and fungi) in the built environment. However, the factors that influenced the prevalence of suspended bacteria and fungi in the air of the built environment are not well recognised. Authorities must take the growth of biological contaminants seriously to prevent serious health effects among occupants of buildings. This review summarises the factors (i.e. outdoor air, dust, ventilation problems, humidity/moisture surface problems and human occupancy) of indoor biological contaminants and their species in buildings that could worsen the indoor air quality. Bacteria species could exist in various indoor buildings (i.e. schools, cafeterias, mosques, faculties, child day-care centres, hospitals, dwellings, educational buildings, homes, elderly care centres, libraries, restaurants, and factories). The dominant bacteria were Bacillus spp. (24.1 %), Staphylococcus spp. (20.7 %), and Micrococcus spp. (20.7 %) whereas for fungi species were Penicillium spp. (25 %), Cladosporium spp. (21.9 %), and Aspergillus spp. (21.9 %).


2014 ◽  
Vol 82 ◽  
pp. 298-306 ◽  
Author(s):  
Edvinas Krugly ◽  
Dainius Martuzevicius ◽  
Ruta Sidaraviciute ◽  
Darius Ciuzas ◽  
Tadas Prasauskas ◽  
...  

Author(s):  
Barend L. van Drooge ◽  
Ioar Rivas ◽  
Xavier Querol ◽  
Jordi Sunyer ◽  
Joan O. Grimalt

Airborne particulate matter with an aerodynamic diameter smaller than 2.5 µg, PM2.5 was regularly sampled in classrooms (indoor) and playgrounds (outdoor) of primary schools from Barcelona. Three of these schools were located downtown and three in the periphery, representing areas with high and low traffic intensities. These aerosols were analyzed for organic molecular tracers and polycyclic aromatic hydrocarbons (PAHs) to identify the main sources of these airborne particles and evaluate the air quality in the urban location of the schools. Traffic emissions were the main contributors of PAHs to the atmospheres in all schools, with higher average concentrations in those located downtown (1800–2700 pg/m3) than in the periphery (760–1000 pg/m3). The similarity of the indoor and outdoor concentrations of the PAH is consistent with a transfer of outdoor traffic emissions to the indoor classrooms. This observation was supported by the hopane and elemental carbon concentrations in PM2.5, markers of motorized vehicles, that were correlated with PAHs. The concentrations of food-related markers, such as glucoses, sucrose, malic, azelaic and fatty acids, were correlated and were higher in the indoor atmospheres. These compounds were also correlated with plastic additives, such as phthalic acid and diisobutyl, dibutyl and dicyclohexyl phthalates. Clothing constituents, e.g., adipic acid, and fragrances, galaxolide and methyl dihydrojasmonate were also correlated with these indoor air compounds. All these organic tracers were correlated with the organic carbon of PM2.5, which was present in higher concentrations in the indoor than in the outdoor atmospheres.


1988 ◽  
Vol 14 (3) ◽  
pp. 213-219 ◽  
Author(s):  
V. Ross Highsmith ◽  
Roy B. Zweidinger ◽  
Raymond G. Merrill

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 641-651 ◽  
Author(s):  
M. Ciganek ◽  
J. Neca

More than one hundred volatile organic substances were identified by gas chromatography and mass spectrometry (GC/MS) in the indoor and outdoor air, stable and farm road dust and farm soil samples from two pig and cattle farms in the South Moravian Region. Volatile fatty acids (acetic, propanoic, butanoic and pentanoic acids) and their esters dominated along with aldehydes (butanal, pentanal and hexanal) and 4-methylphenol in the indoor and outdoor air samples. Road dust and soil samples contained mainly volatile aromatic compounds (toluene, benzene, ethylbenzene, styrene and xylenes), aliphatic hydrocarbons (largely n-alkanes), dichloromethane and carbon disulphide. The health risks associated with particular volatile compounds detected in the indoor and outdoor samples from the farms need to be assessed.


2017 ◽  
Vol 7 (1) ◽  
pp. 14-18
Author(s):  
Nayan Chandra Das ◽  
Farzana Hossaini ◽  
Saurab Kishore Munshi

The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. was predominant. Most of the samples exhibited the presence of Pseudomonas spp. Salmonella spp. and fecal coliform were found each in one sample. Bacillus spp., Escherichia coli, Klebsiella spp. and Vibrio spp. were found in few samples. The average load of the pathogens was 104 cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats. Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18


2017 ◽  
Vol 3 (2) ◽  
pp. 168-174 ◽  
Author(s):  
Arufa Parveen ◽  
Md Mostafizer Rahman ◽  
Md Fakhruzzaman ◽  
Mir Rowshan Akter ◽  
Md Shofiqul Islam

This study was selected to find out the bacterial pathogens in egg yolk, egg shell, feed and air samples of poultry houses at Dinajpur district in Bangladesh with isolation, identification and characterization of bacterial pathogens present in those samples. For this study, a total of 147 samples comprising egg shell (36), egg yolk (36), feed (45) and air (30) were collected during the period from January to May, 2012 and the collected samples were then examined for the bacteriological study by using cultural, morphological and biochemical techniques. On the basis of their cultural, morphological and biochemical properties the isolated organisms were identified as Escherichia coli, Staphylococcus spp., Salmonella serovars and Bacillus spp. In this study it was observed that out of 147 samples a total of 51 were identified as bacterial pathogens in which egg shell containing 10 (27.78%), egg yolk 11 (30.56%), feed 20 (44.44%) and air 10 (33.33%) respectively. In this study it was also observed that the highest prevalence of bacterial pathogens in feed samples (44.44%) in comparison with egg shell (27.78%), egg yolk (30.56%) and air samples (33.33%). In this study it was demonstrated that out of four (04) pathogens Escherichia coli was more abundant (39.21%) in the layer house and its environment in comparison with Staphylococcus spp. (25.49%), Salmonella (23.52%) and Bacillus spp. (11.76%) respectively.Asian J. Med. Biol. Res. June 2017, 3(2): 168-174


2019 ◽  
Vol 24 ◽  
pp. 45-48 ◽  
Author(s):  
Venu Shree ◽  
Bhanu M. Marwaha ◽  
Pamita Awasthi

Two schools in Hamirpur (Himachal Pradesh, India) having hybrid ventilation (ceiling fan) were selected for indoor and outdoor air quality investigation. Investigated parameters include temperature, relative humidity, CO2, and PM2.5 for both indoor and outdoor air quality. The average concentrations of CO2, and PM2.5 are estimated for indoor and outdoor air quality. Result shows that adopted building performance is not good in comparison with designed ones. The indoor concentrations of various pollutants are found to be higher in comparison with outdoor, so there is an urgent need to reduce the levels of pollutants inside the primary classrooms.


2020 ◽  
pp. 1-6
Author(s):  
Wei Qiu ◽  
Qiang Liu ◽  
Wei Zhao ◽  
Langfu Cui ◽  
Binhang Cui

Sign in / Sign up

Export Citation Format

Share Document