scholarly journals ON THE HISTOCHEMISTRY OF AZO GROUP-FREE THIAZOLE DYES

1967 ◽  
Vol 15 (3) ◽  
pp. 172-180 ◽  
Author(s):  
GABRIEL KELIÉNYI
Keyword(s):  
Chemical Composition ◽  
Dye Molecules ◽  
Fluorescent Staining ◽  
Number Of Components ◽  
Related Substances ◽  
Azo Group ◽  
Thioflavine S ◽  
Staining Methods ◽  
Physicochemical Methods ◽  
Thioflavine T

Analysis of primuline, thioflavine S and thioflavine T acid and basic azo group-free thiazole dyes showed that they were built up from a number of components which were characterized by physicochemical methods. It was found that the isolated components, as well as related substances of known chemical composition, have characteristic staining properties. Factors involved in the staining mechanism of the dyes and of components—dye concentration, pH, aggregation of the dye molecules—were investigated and their roles are discussed. Selectivity of these fluorescent staining methods was also studied.

scholarly journals CHROMOSOME COUNT ON YOUNG ANTHER OF BANANA MALE BUD USING EZYMATIC MACERATION AND DAPI STAINING IN SLIDE PREPARATION

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Fajarudin Ahmad ◽  
Yuyu Suryasari Poerba
Keyword(s):  
Genetic Study ◽  
Cell Spreading ◽  
Enzymatic Digestion ◽  
Chromosome Count ◽  
Root Tip ◽  
Chromosome Counting ◽  
Fluorescent Staining ◽  
Dapi Staining ◽  
Slide Preparation ◽  
Staining Methods

Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting. 

Conservation of feline semen: Part II: Cold-induced damages on spermatozoal fertilizing ability

2003 ◽  
Vol 5 (5) ◽  
pp. 257-263 ◽  
Author(s):  
G.C Luvoni ◽  
E Kalchschmidt ◽  
G Marinoni
Keyword(s):  
Special Reference ◽  
Functional Status ◽  
Limiting Factor ◽  
Fluorescent Staining ◽  
Epididymal Sperm ◽  
Freeze Thaw ◽  
Functional Integrity ◽  
Sperm Characteristics ◽  
Fertilizing Ability ◽  
Staining Methods

During the conservation of feline semen, the freeze–thaw procedure in particular is responsible for inducing severe spermatozoal damage, which diminishes fertilizing ability. Therefore, cold-induced damage represents a limiting factor for the conservation of semen, particularly semen from felids, which are often affected by teratospermia. In this article, feline sperm characteristics are reported, with special reference to motility and morphology, which are more likely to be affected by conservation protocols; and moreover, the causes of cold-induced damages are described. Attention has been focused on methods to evaluate functional integrity of spermatozoa, and those applied to cat semen are reviewed. Among these, a rather recently developed technique involves fluorescent staining methods, and in particular chlortetracycline. The chlortetracycline assay applied to cryopreserved cat epididymal sperm shows that it is suitable to evaluate the functional status of cat sperm.

scholarly journals TRENDS IN THE VARIABILITY OF THE CHEMICAL COMPOSITION AND WATER POLLUTION LEVEL IN THE KUBAN RIVER

2021 ◽  
Vol 26 (1) ◽  
pp. 30-40
Author(s):  
O. S. Reshetnyak ◽  
◽  
R. S. Komarov ◽  
◽  
Keyword(s):  
Water Pollution ◽  
Water Quality ◽  
Chemical Composition ◽  
River Water ◽  
Petroleum Products ◽  
Pollution Level ◽  
Organic Substances ◽  
Quality Class ◽  
Number Of Components ◽  
Kuban River

Introduction. The paper explores the long-term spatial and temporal variability of the chemical composition and water quality in the Kuban River. Methods. To study the variability of the chemical composition of river water, we analyzed data from systematic observations over the concentrations of major ions, biogenic and organic substances, petroleum products and heavy metals from 2010 to 2017. To describe the variability of water quality, we used such indicators as water quality class, water pollution level, and characteristic pollutants. Results. It is shown that the spatial change in the chemical composition is uneven — a number of components in the water have low concentrations in the upper reaches, increasing in the lower part of the river. Others are characterized by high concentrations in the middle reaches, followed by a decrease towards the mouth. Over time, the change in the concentrations of chlorides, sulfates, organic substances and petroleum products increases. As for the content of nitrates, a slight decrease was detected in its variability. For the remaining chemicals, there were no clear trends. We established that in most cases the water in the Kuban River can be classified as polluted and very polluted (water quality class 3). We also found that the nature of river water pollution regarding a number of components is stable. Conclusion. In modern conditions of sharp climate changes and anthropogenic impact, the identified features of the chemical composition and trends in water quality variability of the Kuban River are of great practical importance and can be used in the development of environmentally sound recommendations for improving water quality and the state of water ecosystems in the river basin.

scholarly journals THE USE OF BASIC POLYSACCHARIDES IN HISTOCHEMISTRY AND CYTOCHEMISTRY; II. THE DEMONSTRATION OF ACIDIC POLYSACCHARIDES IN TISSUE SECTIONS USING FLUORESCEIN-LABELLED DEACETYLATED CHITIN

1962 ◽  
Vol 10 (1) ◽  
pp. 14-18 ◽  
Author(s):  
SIDNEY P. KENT ◽  
E. EDWARD EVANS
Keyword(s):  
Connective Tissue ◽  
Acridine Orange ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Fluorescent Staining ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Acidic Polysaccharides ◽  
Staining Methods ◽  
Formalin Fixed

A method for demonstrating acidic polysaccharides in formalin fixed parafin embedded tissue sections using fluorescein labelled deacetylated chitin is described. Epithelial and connective tissue acidic polysaccharides have been studied in a number of organs. The distribution of acidic polysaccharides as seen with fluorescein-labelled deacetylated chitin is similar to results obtained with alcian blue-periodic acid Schiff and fluorescein-labelled Aspergillus polysaccharide. Previously reported fluorescent staining methods for acidic polysaccharides such as iron-hematoxylin-acridine orange and atabrine as well as fluorescein-labelled deacetylated chitin may prove to be useful adjuncts to the light microscopic methods of demonstrating mucins. Other possible uses of deacetylated chitin, a colorless macro-cation, in histochemistry and cytochemistry are noted.

scholarly journals COMPARISON OF ZIEHL-NEELSEN, KINYOUN’S AND FLUORESCENT STAINING FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SAMPLES BEFORE AND AFTER PETROFF’S CONCENTRATION TECHNIQUE

2018 ◽  
Vol 11 (4) ◽  
pp. 110
Author(s):  
Neelu Sree P ◽  
Terin J ◽  
Himadri Dutta ◽  
Kalyani M
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sputum Sample ◽  
Diagnostic Value ◽  
Fluorescent Staining ◽  
Concentration Technique ◽  
Before And After ◽  
Auramine O ◽  
Staining Methods ◽  
Staining Techniques ◽  
Microscopy Examination

 Objectives: The objectives of the study were to find out the presence of Mycobacterium tuberculosis in sputum samples using Ziehl-Neelsen (ZN), Kinyoun’s, and (auramine) fluorescent staining and to compare the three staining techniques with and without Petroff’s concentration and to find out the most preferable staining of M. tuberculosis. Methods: Sputum sample was collected and concentrated by 4% NaOH (Petroff’s concentration). Microscopic examination of the sample was done before concentration and after concentration by ZN staining, Kinyoun’s staining, and fluorescent staining (Auramine). Grading of acid-fast bacilli (AFB) by three staining was done before and after concentration according to RNTCP guidelines.Results: Total of 452 sputum samples were collected and subjected to microscopy examination by ZN, Kinyoun’s, and fluorescent staining methods to compare the presence or absence of AFB with or without concentration. Among the 452 sputum samples, total of 67 (15.6%) sputum samples were positive for the presence of AFB. Majority 40.3% (n=21) of TB positive patients were observed in the age group 51–60 years. The results of Auramine-O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100% and specificity 95.6%.Conclusion: The use of fluorescent staining significantly increases the diagnostic value of the smear, particularly where there are low-density bacilli which may escape detection on ZN stained smears.

Preparation of Copper Electroerosion Nanopowders from Waste of Aquatic Medium and its Validation by Physicochemical Methods

2015 ◽  
Vol 770 ◽  
pp. 23-27 ◽  
Author(s):  
E.V. Ageev ◽  
I.A. Avilova ◽  
N.M. Horyakova
Keyword(s):  
Chemical Composition ◽  
Copper Powder ◽  
Copper Wire ◽  
Powder Materials ◽  
Aquatic Medium ◽  
Powder Particles ◽  
Physicochemical Methods ◽  
Average Size ◽  
Electroerosion Dispersion

The article is devoted to the modes of getting copper powder materials by the method of electroerosion dispersion from waste of an electrotechnical copper wire of an aquatic environment. The results of determination of an average size of powder particles, forms and morphology of copper powder particles and the chemical composition of a powder.

scholarly journals Investigation of peloid from some lake in Zavkhan province

2018 ◽  
pp. 26-32
Author(s):  
Bayaraa B ◽  
Nomintsetseg B ◽  
Ganzaya G ◽  
Ankhzaya A` ◽  
Dolmaa G
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Bottom Sediment ◽  
Humic Substances ◽  
Polar Solvents ◽  
Total Extract ◽  
X Ray ◽  
Sea Bottom ◽  
Physicochemical Methods

The purpose of study was to determine chemical composition and organic matter of peloids from lakes located named as Tsagaan Lake and toirom of Tsagaan Lake in Zavkhan province by chemical and modern physicochemical methods, including (X-ray, IR and ICP-AES). From the result, peloid from Tsagaan Lake was identified that peloid belongs to silt sulphide muddy type whereas characterization of peloid from toirom of Tsagaan Lake was almost similar with peloid knoll, but it is not possible to use the treatment and peloid solution was 5 to 6 times higher than the category of much pollutant. 45 elements determined in two Lakes peloid´s samples. The content of some macro elements (Al, Fe, Mg, P, Ba and K) were lower than Clark elements of sea bottom sediment whereas the content of Na, Ba were higher than it. Lipid and carbohydrate contains were between 0.03 - 0.29%, and 0.005-0.04%. Humic substances content varied between 0.19 % - 0.71% in these peloids. Organic matters, which are free, associated with minerals and absorbed extracted in peloids using non-polar and polar solvents; yield of total extract in peloids were 0.2781%, 0.4161% respectively. Завхан аймгийн зарим нуурын шаврын судалгааны дүнгээс Хураангуй: Завхан аймгийн Цагаан нуурын тойром, Цагаан нуурын шавар, шаврын уусмалын ерөнхий үзүүлэлтүүд, органик бодисын агуулга, эрдэс, элементийн найрлагыгхимийн болон физик-химийн орчин үеийн судалгааны аргуудыг (ИК, РД, ICP-AES) ашиглан судлахад Цагаан нуурын шавар нь хүхэртустөрөгчит лаг шаврын ангилалын үзүүлэлттэй, Цагаан нуурын тойрмын шавар нь бялхамал шаврын үзүүлэлтүүдтэй ойролцоо байгаа ч эмчилгээнд хэрэглэх боломжгүй бөгөөд шаврын уусмал нь маш их бохирдолттой гэсэн ангилалаас 5-6 дахин их байгааг илрүүлэн тогтоов. Судалгаанд хамрагдсан 2 нуурын эмчилгээний шаварт нийт 45 элемент бүртгэгдсэнээс зарим макроэлементүүд (Al, Fe, Mg, P, Ba, K) далайн ёроолын тунамал хурдасын кларк хэмжээнээс бага, харин Na, Ba агуулга их байна. Цагаан нуурын шаварт гумины бодис 0.71 %, липид 0.03 %, нүүрс-ус 0.04 %, Цагаан нуурын тойрмын шаварт гумины бодис 0.19 %, липид 0.29 %, нүүрс-ус 0.005 % тус тус агуулгатай байна. Шаврын чөлөөт органик нэгдэл, эрдэстэй холбогдсон болон тэдгээрт шингээгдсэн органик бодисын нийлбэр агуулга Цагаан нуурын шаварт 0.2781 %, Цагаан нуурын тойрмын шаварт 0.4161 % байгааг судлан тогтоов. Түлхүүр үг: пелоид, гумины бодис, липид, бальнеологи

STUDIES ON THE BIONOMICS AND CONTROL OF THE BURSATE NEMATODES OF HORSES AND SHEEP: III. FURTHER OBSERVATIONS ON THE TOXICITY OF URINE AND SOME RELATED SUBSTANCES FOR SCLEROSTOME LARVAE IN FECES

1936 ◽  
Vol 14d (11) ◽  
pp. 172-180 ◽  
Author(s):  
I. W. Parnell
Keyword(s):  
Chemical Composition ◽  
Lethal Effect ◽  
Farm Animals ◽  
Comparative Tests ◽  
Related Substances ◽  
Cow Urine ◽  
And Control

Comparative tests with cow urine show that there is considerable variation in its lethal effect on horse sclerostome larvae in manure. Urine gradually loses its lethal power over a period of months and is useless after a year. Tests on urines of various farm animals are also recorded, as well as on fluid from byres and manure heaps. The chemical composition of urine is discussed and tests with ammonia and acetone on larvae are described.

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