Protective Effect of the Dental Pellicle against Erosive Challenges in situ

The acquired dental pellicle helps prevent erosion, but the protection level is unknown. This in situ study tested whether a two-hour pellicle protects against different erosive challenges by orange juice. Subjects wore palatal appliances loaded with either enamel or dentin specimens. Pellicle was allowed to form, or not (control), on the surfaces of the specimens intra-orally for 2 hrs before the erosive challenges of 0 (control), 10, 20, and 30 minutes’ duration. Specimens were randomly removed from the appliances after each challenge. Percentage of surface microhardness change (%SMC) was determined for the enamel specimens, and that of mineral loss and lesion depth for the dentin specimens. Enamel specimens with the pellicle showed a significantly lower %SMC, only after the 10-minute challenge. No protection was found for dentin. It was concluded that the acquired pellicle reduced dental erosion, but that this effect was limited to the less severe erosive challenge on enamel surfaces.

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Anticaries Potential of a Sodium Monofluorophosphate Dentifrice Containing Calcium Sodium Phosphosilicate: Exploratory in situ Randomized Trial

Caries Research ◽

10.1159/000453622 ◽

2017 ◽

Vol 51 (2) ◽

pp. 170-178 ◽

Cited By ~ 3

Author(s):

Charles R. Parkinson ◽

Muhammad Siddiqi ◽

Stephen Mason ◽

Frank Lippert ◽

Anderson T. Hara ◽

...

Keyword(s):

Bioactive Glass ◽

Randomized Trial ◽

Dentin Hypersensitivity ◽

Surface Microhardness ◽

Single Centre ◽

Lesion Depth ◽

In Situ Study ◽

Caries Lesions ◽

Mineral Loss

Calcium sodium phosphosilicate (CSPS) is a bioactive glass material that alleviates dentin hypersensitivity and is postulated to confer remineralization of caries lesions. This single-centre, randomized, single (investigator)-blind, placebo-controlled, crossover, in situ study explored whether the addition of 5% CSPS to a nonaqueous fluoride (F) such as sodium monofluorophosphate (SMFP)-containing dentifrice affects its cariostatic ability. Seventy-seven subjects wore 4 gauze-covered enamel specimens with preformed lesions (2 surface-softened and 2 subsurface) placed buccally on their mandibular bilateral dentures for up to 4 weeks. Subjects brushed twice daily with 1 of the 5 study dentifrices: 927 ppm F/5% CSPS, 927 ppm F/0% CSPS, 250 ppm F/0% CSPS, 0 ppm F/5% CSPS, or 0 ppm F/0% CSPS. Specimens were retrieved after either 21 (surface-softened lesions; analyzed by Knoop surface microhardness [SMH]) or 28 days (subsurface lesions; analyzed by transverse microradiography). The enamel fluoride uptake was determined for all specimens using a microbiopsy technique. The concentrations of fluoride and calcium in gauze-retrieved plaque were also evaluated. Higher dentifrice fluoride concentrations led to greater remineralization and fluoridation of both lesion types and increased plaque fluoride concentrations. CSPS did not improve the cariostatic properties of SMFP; there were no statistically significant differences between 927 ppm F/5% CSPS and 927 ppm F/0% CSPS in percent SMH recovery (p = 0.6788), change in integrated mineral loss (p = 0.5908), or lesion depth (p = 0.6622). Likewise, 0 ppm F/5% CSPS did not provide any benefits in comparison to 0 ppm F/0% CSPS. In conclusion, CSPS does not negatively impact nor does it improve the ability of an SMFP dentifrice to affect remineralization of caries lesions.

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Re- and Demineralization Characteristics of Enamel Depending on Baseline Mineral Loss and Lesion Depth in situ

Caries Research ◽

10.1159/000444537 ◽

2016 ◽

Vol 50 (2) ◽

pp. 141-150 ◽

Cited By ~ 10

Author(s):

Richard J. Wierichs ◽

Julian Lausch ◽

Hendrik Meyer-Lueckel ◽

Marcella Esteves-Oliveira

Keyword(s):

Negative Control ◽

Pronounced Change ◽

Protocol Violation ◽

Lesion Depth ◽

Treatment Groups ◽

Before And After ◽

Double Blinded ◽

Bovine Enamel ◽

Mineral Loss

Objectives: The aim of this double-blinded, randomized, cross-over in situ study was to evaluate the re- and demineralization characteristics of sound enamel as well as lowly and highly demineralized caries-like enamel lesions after the application of different fluoride compounds. Methods: In each of three experimental legs of 4 weeks, 21 participants wore intraoral mandibular appliances containing 4 bovine enamel specimens (2 lowly and 2 highly demineralized). Each specimen included one sound enamel and either one lowly demineralized (7 days, pH 4.95) or one highly demineralized (21 days, pH 4.95) lesion, and was positioned 1 mm below the acrylic under a plastic mesh. The three randomly allocated treatments (application only) included the following dentifrices: (1) 1,100 ppm F as NaF, (2) 1,100 ppm F as SnF2 and (3) 0 ppm F (fluoride-free) as negative control. Differences in integrated mineral loss (ΔΔZ) and lesion depth (ΔLD) were calculated between values before and after the in situ period using transversal microradiography. Results: Of the 21 participants, 6 did not complete the study and 2 were excluded due to protocol violation. Irrespectively of the treatment, higher baseline mineral loss and lesion depth led to a less pronounced change in mineral loss and lesion depth. Except for ΔΔZ of the dentifrice with 0 ppm F, sound surfaces showed significantly higher ΔΔZ and ΔLD values compared with lowly and highly demineralized lesions (p < 0.05, t test). Conclusion: Re- and demineralization characteristics of enamel depended directly on baseline mineral loss and lesion depth. Treatment groups should therefore be well balanced with respect to baseline mineral loss and lesion depth.

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The Erosive Potential of Different Malt Drinks: An in vitro and in situ study

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.33.1.y068425317v40j22 ◽

2008 ◽

Vol 33 (1) ◽

pp. 35-37 ◽

Cited By ~ 2

Author(s):

Esber Çaglar ◽

Sule Kavaloglu Cildir ◽

Nuket Sandalli

Keyword(s):

Potential Effect ◽

Dental Erosion ◽

Variable Degree ◽

Water Control ◽

In Situ Study ◽

Significant Difference ◽

Natural Spring ◽

Erosive Effect

Objectives: Whereas the potential effect of acidic drinks in the etiology of dental erosion is well recognized the role of malt drinks is unclear. The primary aim of the present study was to compare the in vitro erosive effect on enamel produced by different aromated malt drinks. A secondary objective was to compare their erosive effects in situ with those determined in vitro. Materials and methods: To select the malt drink for the study in situ, six commercially available malt drinks were examined for erosive potential in vitro. The study in situ was a single centre, 2-period, 2-treatment crossover study to compare the erosive effect of a commercially available malt drink (Test), with that of natural spring water (Control), over 10 day periods on 10 healthy volunteers. Subjects wore upper removable appliances containing two human enamel specimens from 9 a.m. to 4 p.m. The regimen of intake of the drinks was 250 ml at midday. Measurements of enamel loss were made on samples after 5 and 10 days by profilometry. Results: The in situ study showed a statistically significant difference in erosive potential between the test and control beverages. No specimen exposed to the control beverage displayed appreciable erosion. Erosion occurred with the test drink, but to a variable degree between subjects. Conclusions: Malt drinks should be considered as potentially erosive as the results for enamel specimens exposed to the test beverage in the clinical study showed a degree of erosion that varied greatly between different participants. It is likely that under these conditions an increase in the degree of erosion would be observed in children and young people who consume malt drinks.

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Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study

Brazilian Oral Research ◽

10.1590/1807-3107bor-2021.vol35.0108 ◽

2021 ◽

Vol 35 ◽

Author(s):

Maria Denise Rodrigues DE MORAES ◽

Vanara Florêncio PASSOS ◽

Gislaine Cristina PADOVANI ◽

Lady Clarissa Brito da Rocha BEZERRA ◽

Ilka Maria VASCONCELOS ◽

...

Keyword(s):

Protective Effect ◽

Green Tea ◽

Tea Catechins ◽

In Situ Study ◽

Green Tea Catechins ◽

Human Dentin

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Inhibition of enamel mineral loss by fissure sealant: An in situ study

Journal of Dentistry ◽

10.1016/j.jdent.2012.09.015 ◽

2013 ◽

Vol 41 (1) ◽

pp. 42-50 ◽

Cited By ~ 8

Author(s):

Kamila Rosamilia Kantovitz ◽

Fernanda Miori Pascon ◽

Francisco Humberto Nociti ◽

Cinthia P. Machado Tabchoury ◽

Regina Maria Puppin-Rontani

Keyword(s):

Fissure Sealant ◽

In Situ Study ◽

Mineral Loss

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In Situ Investigation of the Remineralizing Effect of Saliva and Fluoride on Enamel Following Prophylaxis Using Sodium Bicarbonate

European Journal of Dentistry ◽

10.1055/s-0039-1698857 ◽

2011 ◽

Vol 05 (01) ◽

pp. 040-046 ◽

Cited By ~ 2

Author(s):

Gladis Benjamina Grazziotin ◽

Daniela Rios ◽

Heitor Marques Honório ◽

Salete Moura Bonifácio Silva ◽

José Eduardo Oliveira Lima

Keyword(s):

Sodium Bicarbonate ◽

Surface Wear ◽

Surface Microhardness ◽

Mean Values ◽

In Situ Study ◽

Enamel Structure ◽

In Situ Investigation ◽

Paired T Test

ABSTRACTObjectives: This in situ study evaluated the effect of saliva, associated or not with fluoride, on enamel previously submitted to prophylaxis using sodium bicarbonate. Methods: The study was conducted on enamel blocks submitted to in vitro prophylaxis using sodium bicarbonate. The blocks were randomly divided into 2 groups (G1/G2) and mounted on intraoral appliances wore by 10 volunteers. G1 blocks were directly exposed to saliva in situ, while blocks in G2 were exposed to saliva with fluoride (rinsing with 0.2% NaF solution during the initial minute). Enamel alterations were evaluated using surface microhardness and profilometry. Enamel hardness data were analyzed by ANOVA and Tukey tests and surface wear was evaluated using paired t test (P<.05). Results: No significant differences were found between G1 and G2 for enamel hardness and wear. The wear after prophylaxis was not different from the wear after the in situ stage. Baseline mean values of enamel hardness, after prophylaxis and after the in situ stage were 340±16.6, 329±35.7 and 354±37.8 for G1 and 338±15.6, 312±46.3 and 340±21.8 for G2, respectively. Conclusions: It was concluded that saliva alone exhibited a similar effect to saliva associated with fluoride; after 4h of in situ remineralization, there was no recovery in height of the enamel structure that had been lost due to the application of sodium bicarbonate. (Eur J Dent 2011;5:40-46)

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Resin-Based Materials Protect Against Erosion/Abrasion—a Prolonged In Situ Study

Operative Dentistry ◽

10.2341/17-198-l ◽

2019 ◽

Vol 44 (3) ◽

pp. 302-311

Author(s):

D Rios ◽

GC Oliveira ◽

CR Zampieri ◽

MC Jordão ◽

EJ Dionisio ◽

...

Keyword(s):

Dental Erosion ◽

Additional Treatment ◽

The Other ◽

Control Group ◽

Material Loss ◽

In Situ Study ◽

Phase Study ◽

And Control ◽

Bovine Enamel

SUMMARY While patient compliance is key to preventive measures related to dental erosion, the application of resin-based materials could serve as an additional treatment to inhibit erosion progression. This in situ study evaluated the effect of applying resin-based materials, including resin infiltrant, on previously eroded enamel subjected to prolonged erosive and abrasive challenges. The factors under study were types of treatment (infiltrant [Icon], sealant [Helioseal Clear], adhesive [Adper Scotchbond Multi-Purpose Plus], and control [no treatment]); wear conditions (erosion [ERO] and erosion + abrasion [ERO + ABR]) and challenge time (5 and 20 days) in a single-phase study. The blocks were prepared from bovine enamel, eroded (0.01 M HCl, pH 2.3 for 30 seconds) and randomized among treatments, wear conditions, and volunteers. The application of resin-based materials followed the manufacturers' recommendations. Twenty-one volunteers wore the palatal intraoral device, in which one row corresponded to ERO and the other to ERO + ABR. In each row, all treatments were represented (2 blocks per treatment). For 20 days, the erosive challenge was performed 4 times/day (immersion in 0.01 M HCl, pH 2.3, for 2 minutes) for the ERO condition. For the ERO + ABR condition, two of the erosive challenges were followed by abrasion for 15 seconds with fluoride dentifrice slurry. Enamel and/or material loss was measured using profilometry (initial, after treatment, and after the end of the fifth and 20th days of in situ erosive challenge) and analyzed by ANOVA models and Tukey's test (α=0.05). The results showed that the application of resin-based materials did not cause superficial enamel loss. The infiltrant group showed a thicker layer of material above the enamel compared with the other materials (p=0.001). After the erosive challenge, there was no difference between the conditions ERO and ERO + ABR (p=0.869). All materials protected the enamel against erosion progression compared with the control group (p=0.001). Based on these results, we conclude that the application of resin-based materials results in protection of previously eroded enamel subjected to in situ erosive and abrasive challenge for 20 days.

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