Cell Kinetics and Tumor-Associated Antigen Expression in Human Mammary Carcinomas

1991 ◽  
Vol 6 (3) ◽  
pp. 159-166 ◽  
Author(s):  
A. Contegiacomo ◽  
R. Mariani Costantini ◽  
R. Muraro ◽  
P. Battista ◽  
C. Valli ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Proliferative Activity ◽  
Antigen Expression ◽  
Breast Carcinomas ◽  
Cellular Kinetics ◽  
Tumor Associated Antigen ◽  
Antigenic Expression ◽  
High Proliferative Activity ◽  
Antigenic Phenotype ◽  
Intense Immunoreactivity

Twenty-six primary breast carcinomas were studied to evaluate cell proliferation as assessed by thymidine labeling index (TLI), and antigenic phenotype, as defined by immunohistochemistry using eight monoclonal antibodies (MAbs) to tumor-associated antigens (TAAs). The majority of tumors had low TLI values. Reactivity to MAbs B72.3, CC49, CC83 (anti TAG 72), COL-12 (anti CEA) and MOv2 (against a tumor-associated mucoprotein) was restricted to < 50% of the tumors studied, while MAbs B1.1 (anti CEA), MBrl and MBr8 (to tumor-associated carbohydrates) reacted with > 50% of the cases. Correlations between expression of TAAs and proliferative activity showed that the tumors could be divided into three groups, two characterized by either high proliferative activity and absence of antigenic expression or low proliferative activity and strong antigenic expression, and the third showing no relation between these two biological features. We defined two antigenic phenotypes associated with specific cellular kinetics: one characterized by negative immunoreaction with MAbs, CC49, CC83 and COL-12 and high proliferative activity; the other characterized by intense immunoreactivity with these antibodies and low proliferative activity. The data suggest that cell proliferation and antigenic phenotype may define biologic subsets of breast carcinomas

Expression of proliferation genes in formalin-fixed paraffin-embedded (FFPE) tissue from breast carcinomas. Feasibility and relevance for a routine histopathology laboratory

2016 ◽  
Vol 70 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Carla Thomas ◽  
Cleo Robinson ◽  
Ben Dessauvagie ◽  
Benjamin Wood ◽  
Greg Sterrett ◽  
...  
Keyword(s):  
Gene Expression ◽  
Breast Carcinoma ◽  
Expression Profiling ◽  
Proliferative Activity ◽  
Breast Cancers ◽  
Breast Carcinomas ◽  
Ki 67 ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

AimBreast carcinoma proliferative activity, histological grade and commercial molecular tests are all important in prognostication and treatment. There is a particular need for improved, standardised techniques for subclassification of grade 2 breast cancers into low-risk and high-risk prognostic groups. In this study we investigated whether gene expression profiling of five proliferation genes was feasible using breast cancer tissue in a clinical setting and whether these profiles could enhance pathological assessment.MethodsExpression of five proliferation gene mRNAs; Ki-67, STK 15, CCNB1, CCND1 and MYBL2, was quantified in 27 breast carcinomas and compared with Ki-67 proliferation index (PI) and Nottingham mitotic score.ResultsExpression of Ki-67, STK15 and MYBL2 mRNA showed moderate Spearman's correlation with Ki-67 PI (p<0.01), but CCND1 and CCNB1 showed weak, non-significant correlation. Individual gene expression did not associate with mitotic score but combined mRNA expression correlated with both Ki-67 PI (p=0.018) and mitotic score (p=0.03; 0.007).ConclusionsThis study confirms mRNA analysis in breast carcinoma formalin-fixed, paraffin-embedded samples is feasible and suggests gene expression profiling, using a small set of five proliferation genes, has potential in aiding histological grading or assessment of proliferative activity of breast cancers. To fully evaluate the clinical applicability of this approach, a larger cohort study with long-term follow-up data is required.

scholarly journals Abstract 4694: Evaluation of tumor-associated antigen expression with the MACSimaTMhigh-content imaging platform

2019 ◽  
Author(s):  
Christoph Herbel ◽  
Vera Dittmer ◽  
Manuel Martinez-Osuna ◽  
Laura Nadine Kuester ◽  
Daniel Schaefer ◽  
...  
Keyword(s):  
Antigen Expression ◽  
Tumor Associated Antigen

Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers

2007 ◽  
Vol 24 (1) ◽  
pp. 27-37 ◽  
Author(s):  
V. S. Sarafian ◽  
Y. Uzunova ◽  
S. Hayrabedyan ◽  
P. Ganchevska ◽  
M. Filipova ◽  
...  
Keyword(s):  
Blood Group ◽  
Proliferative Activity ◽  
Antigen Expression ◽  
Blood Group Antigen

scholarly journals Regulation of human monocyte HLA-DR and CD4 antigen expression, and antigen presentation by 1,25-dihydroxyvitamin D3

Blood ◽  
1990 ◽  
Vol 76 (1) ◽  
pp. 189-197 ◽  
Author(s):  
WF Rigby ◽  
M Waugh ◽  
RF Graziano
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Antigen Presentation ◽  
Antigen Expression ◽  
Human Monocyte ◽  
T Cell Proliferation ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Hla Dr ◽  
And Function

Abstract 1,25-Dihydroxyvitamin D3 (1,25(OH)2-D) has been shown to be a macrophage-derived cytokine, capable of regulating myeloid differentiation and T-cell activation in vitro. Therefore, we examined the effects of 1,25(OH)2-D on the monocyte phenotype and function of human peripheral blood monocytes as an index of its biologic role at an inflammatory site. 1,25(OH)2-D treatment consistently and specifically reduced HLA-DR and CD4 expression by monocytes, while CD14 and class I HLA antigen expression were unaffected. Expression of Fc gamma R I-III on monocytes was variably modulated by 1,25(OH)2-D treatment, but no differences in antibody-dependent cell cytotoxicity (ADCC) were observed, measured using either ADCC or anti-Fc gamma R-antibody expressing hybridomas. In contrast, the ability of monocytes to induce antigen-dependent T-cell proliferation was markedly reduced by 1,25(OH)2-D pretreatment for as little as 6 hours. Addition of interleukin-1 (IL-1), IL-6, or indomethacin did not restore antigen- dependent T-cell proliferation, suggesting that this observation was not secondary to changes in IL-1, IL-6, or PGE2 production induced by 1,25(OH)2-D. These data suggest that 1,25(OH)2-D treatment specifically modulates human monocyte phenotype and function, altering HLA-DR antigen expression and antigen presentation, while leaving lytic function intact. These findings may be relevant to the immunobiologic role of 1,25(OH)2-D.

scholarly journals Comparative Evaluation of the Clinical Efficacy of PRP-Therapy, Minoxidil, and Their Combination with Immunohistochemical Study of the Dynamics of Cell Proliferation in the Treatment of Men with Androgenetic Alopecia

2020 ◽  
Vol 21 (18) ◽  
pp. 6516
Author(s):  
Elena E. Pakhomova ◽  
Irina O. Smirnova
Keyword(s):  
Cell Proliferation ◽  
Clinical Efficacy ◽  
Comparative Evaluation ◽  
Proliferative Activity ◽  
Immunohistochemical Study ◽  
Platelet Rich Plasma ◽  
Androgenetic Alopecia ◽  
Hair Morphology ◽  
Promising Treatment ◽  
Cd34 Expression

Platelet-rich plasma (PRP) therapy has been considered as a promising treatment for androgenetic alopecia (AGA). The aim of the study was comparative evaluation of the clinical efficacy of PRP-therapy, minoxidil, and their combination in the treatment of men with AGA and to evaluate the effects of PRP on the proliferation of hair follicle (HF) cells in skin biopsy. Materials and Methods: The study involved 69 men who were divided into 3 groups who received PRP therapy, minoxidil, and their combination. The clinical efficacy of the therapy was evaluated by the dynamics of morphometric of hairs. To assess cell proliferation antibodies to β-catenin, CD34, Ki67, and to Dkk-1 were used. Results. PRP treatment was more effective than minoxidil therapy (p = 0.005). Complex therapy turned out to be more effective than minoxidil monotherapy (p < 0.0001) and PRP monotherapy (p = 0.007). After applying PRP the absolute and relative values of the β-catenin and CD34 expression area increased; an increase in Ki67+ index was also significant. Conclusions: PRP can be considered as a treatment option for AGA. Combined PRP and minoxidil use seems promising for the treatment of AGA. PRP increase in the proliferative activity of HF cells and improves hair morphology in patients with AGA.

Immunohistochemical Distribution of Mucinous-like Carcinoma Associated Antigen (MCA) in Breast and Non-breast Cancer: Comparison with other Biological Parameters

1990 ◽  
Vol 5 (3) ◽  
pp. 138-144 ◽  
Author(s):  
M. Martinazzi ◽  
F. Crivelli ◽  
C. Grassi ◽  
C. Zampatti
Keyword(s):  
Proliferative Activity ◽  
Progesterone Receptors ◽  
Growth Fraction ◽  
Human Tissues ◽  
Biological Parameters ◽  
Breast Carcinomas ◽  
Epithelial Growth Factor ◽  
Cancer Tissues ◽  
P 53 ◽  
Epithelial Growth

The present study reports the immunohistochemical reactivity of the monoclonal antibody b-12 (MAb b-12) with malignant human tissues. 173 neoplastic tissues were tested: MAb b-12 stained all breast carcinomas independently of their histology, with different patterns within the various type of cancer. Some other carcinomas (stomach, bowel, ovary, lung, endometrium), were also reactive even if the fraction of positive cells was lower. A comparison between the histological localization of MCA and that of CEA was performed; anti-CEA antibodies stained the cancer tissues with different reactivity and showed different percentages of positivity. MCA expression was also compared with other biological parameters such as the presence of estrogen receptors (ER), progesterone receptors (PgR), epithelial growth factor receptors (EGF-R), and oncoprotein p-53 which is encoded by the oncogene N-myc. The proliferative activity was also evaluated by measuring the growth fraction (GF) using the antibody Ki67. Any correlation was demonstrated between MCA and these parameters except for growth fraction as revealed by Ki67 antibody.

scholarly journals BrdU Pulse LabellingIn Vivoto Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
B. Yahaya ◽  
G. McLachlan ◽  
D. D. S. Collie
Keyword(s):  
Cell Proliferation ◽  
Proliferative Activity ◽  
Histological Analysis ◽  
S Phase ◽  
Pulse Labelling ◽  
Airway Wall ◽  
Brush Biopsy ◽  
Cell Proliferative Activity ◽  
Airway Tree

The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness ofin vivopulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility of stem cell approaches in relevant lung disease models.

scholarly journals Elevated Tumor-Associated Antigen Expression Suppresses Variant Peptide Vaccine Responses

2011 ◽  
Vol 187 (9) ◽  
pp. 4431-4439 ◽  
Author(s):  
Charles B. Kemmler ◽  
Eric T. Clambey ◽  
Ross M. Kedl ◽  
Jill E. Slansky
Keyword(s):  
Peptide Vaccine ◽  
Antigen Expression ◽  
Vaccine Responses ◽  
Tumor Associated Antigen ◽  
Variant Peptide
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