12-Membered Resorcylic Acid Lactones Isolated from Saccharicola bicolor, an Endophytic Fungi from Bergenia purpurascens

Two new resorcylic acid lactones, 13-hydroxyhidroresorcylide (1) and 12-hydroxyhidroresorcylide (2), along with four known congeners (3–6) were isolated from Saccharicola bicolor, an endophytic fungus from Bergenia purpurascens. Their structures were elucidated by interpretation of the spectroscopic evidence.

Download Full-text

Isolation of Endophytic Fungi QPS 05 from Quercus phillyraeoides A. Gray and Its Potential for α-Glucosidase Inhibitory Activity

Jurnal Kimia Terapan Indonesia ◽

10.14203/jkti.v20i1.372 ◽

2018 ◽

Vol 20 (1) ◽

pp. 1-7

Author(s):

Anastasia Wheni Indrianingsih ◽

Amalia Indah Prihantini ◽

Sanro Tachibana

Keyword(s):

Fatty Acids ◽

Endophytic Fungi ◽

Inhibitory Activity ◽

Endophytic Fungus ◽

Inhibitory Concentration ◽

Life Cycles ◽

Ic50 Values ◽

Quercus Phillyraeoides ◽

Alternaria Sp ◽

Harmful Effects

AbstractEndophytic fungi are the microorganisms that spend all or part of their life cycles within plant tissue without causing harmful effects on the plant. In this study, 14 endophytic fungus from Quercus phillyraeoides A. Gray were isolated. Alternaria sp. QPS 05, an endophytic fungi which was isolated from the stem of Q. phillyraeoides A. Gray showed the highest α-glucosidase inhibitory activity. Further separation of ethyl acetate extract from the fungus led to the isolation of active substance from hexane-soluble fraction which give fatty acids mixture consist of palmitic acid, oleic acid, linoleic acid and linolenic acid (1) strong inhibitory activity against α-glucosidase. Isolated fatty acids (1) had inhibitory concentration (IC50) values against Saccharomyces cerevisiae was 12.10 μg/mL. The results of the present study showed that endophytic fungus from Alternaria sp. QPS 05 potentially contained a rich source of natural antidiabetic medicine.

Download Full-text

A Phenolic Compound of Endophytic Fungi Isolated from Stem of Syzygium aqueum and its Diuretic Activity

Biointerface Research in Applied Chemistry ◽

10.33263/briac125.70407051 ◽

2021 ◽

Vol 12 (5) ◽

pp. 7040-7051

Keyword(s):

Secondary Metabolites ◽

Phenolic Compound ◽

Endophytic Fungi ◽

Endophytic Fungus ◽

White Male ◽

Urine Volume ◽

Chloride Ions ◽

Male Rats ◽

Diuretic Activity ◽

Fungus Extract

Guava (Syzygium aqueum), a typical Indonesian crop plant, has diuretic properties. Plants used as diuretic medicines can also be valuable sources for endophytic fungi containing diuretic drugs. The aims of this study were to isolate and identify endophytic fungi from S. aqueum stems, to isolate their secondary metabolites, and to conduct in vivo tests of the diuretic activity of an endophytic fungus extract on white male rats of the Wistar strain. Endophytic fungi were isolated by a direct plating method, and fungal isolates were identified molecularly. A phenolic compound was isolated by chromatography, and the chemical structure was identified spectroscopically. Doses of 100, 200, and 300 mg/kg BW were administered to white male rats divided into six groups (normal control, negative control, positive control, and the three treatment groups). Diuretic tests included urine volume; sodium, chloride, and calcium electrolyte levels; and pH. Phylogenetic analysis identified the endophytic fungal isolate as Trichoderma ghanense (isolation code SA1). The secondary metabolite isolated from the T. ghanense extract was a phenolic compound. Diuretic tests using the endophytic fungus extract showed the highest urine volume with a dose of 300 mg/kg BW. The diuretic test results showed an increase in urine volume and levels of sodium, potassium, and chloride ions in the urine. The extract of T. ghanense isolated from the stem of S. aqueum has the potential as initial therapy for hypertension because it contains phenolic secondary metabolites that show diuretic activity.

Download Full-text

Two New Isocoumarin Derivatives from an Endophytic Fungi Pestalotiopsis coffeae Isolated from a Mangrove Fishtail Palm

Natural Product Communications ◽

10.1177/1934578x1801300117 ◽

2018 ◽

Vol 13 (1) ◽

pp. 1934578X1801300 ◽

Cited By ~ 2

Author(s):

Zhong Wang ◽

Peng Fan ◽

Tong-Dan Xue ◽

Lin-Lin Meng ◽

Wen-Bin Gao ◽

...

Keyword(s):

Carboxylic Acid ◽

Endophytic Fungi ◽

Endophytic Fungus ◽

Spectroscopic Data ◽

X Ray Diffraction ◽

X Ray ◽

Chinese Plant

Two new isocoumarin derivatives, 6,8-dihydroxy-7-methyl-1-oxo-1H-isochromene-3-carboxylic acid (1) and 6,8-dihydroxy-3-methoxy-3,7- dimethyl-isochroman-1-one (2), together with five known compounds (3-7), were isolated from the endophytic fungus Pestalotiopsis coffeae derived from the Chinese plant fishtail palm. The structures of these compounds were determined mainly by analysis of their NMR spectroscopic data. The structure of compound 2 was further confirmed by X-ray diffraction.

Download Full-text

A Novel Sesquiterpene Alcohol from Fimetariella Rabenhorstii, an Endophytic Fungus of Aquilaria sinensis

Natural Product Communications ◽

10.1177/1934578x1100600605 ◽

2011 ◽

Vol 6 (6) ◽

pp. 1934578X1100600

Author(s):

Mei-Hua Tao ◽

Jian Yan ◽

Xiao-Yi Wei ◽

Dong-Li Li ◽

Wei-Min Zhang ◽

...

Keyword(s):

Endophytic Fungi ◽

Endophytic Fungus ◽

Spectroscopic Methods ◽

Formation Processes ◽

Aquilaria Sinensis ◽

Liquid Cultures ◽

Sesquiterpene Alcohol

A novel sesquiterpene alcohol, named frabenol (1), was isolated from liquid cultures of Fimetariella rabenhorstii A20, an endophytic fungus of the agarwood-forming plant Aquilaria sinensis (Lour.) Gilg. The structure of the new compound was elucidated on the basis of spectroscopic methods. The finding of a sesquiterpenoid compound in F. rabenhorstii A20 implied that endophytic fungi of agarwood-producing plants could also contribute to the generation of fragrant chemicals during the agarwood formation processes.

Download Full-text

The Potency of Endofit Fungi in Cocoa as Biological Agent to Control Cocoa Pod Disease Caused by Phytophthota Palmivora (Butler) Butler

Advances in Tropical Biodiversity and Environmental Sciences ◽

10.24843/atbes.2017.v01.i01.p02 ◽

2017 ◽

Vol 1 (1) ◽

pp. 6

Author(s):

I Made Sudarma ◽

Ni Made Puspawati ◽

I Ketut Suada

Keyword(s):

Endophytic Fungi ◽

Endophytic Fungus ◽

Fungal Endophyte ◽

Biological Agent ◽

Phytophthora Palmivora ◽

In Vitro Test ◽

Trichoderma Spp ◽

Endophyte Fungi

Cocoa pod disease caused by the fungus Phytophthora palmivora has resulted in loss of cacao in Indonesia, especially in Bali. So far the disease control strategy is not fully understood. So there is a need to find an alternative by using endophytic fungi associated with cocoa plant. Endophytic fungi are needed to be explored in all parts of the cocoa plant such as stems, leaves and husks. The prevalence of fungal endophyte was determined by the size of its domination on the surface tissue for protection against pathogen. The exploration of the endophytic fungi benefits is aimed at finding biological agent s that could control of pathogenic P. palmivora. The results showed that 15 types of endophyte fungi have been found in the healthy leaves, stem and pod husks, with the prevalence of fungal endophyte originated from healthy leaf Mecelia sterilia (hyphae sterile) around 30%, the endophyte fungi originated from the healthy cocoa stem are Mycelia sterilia, Neurospora spp and Trichoderma spp around 25%. While the endophytic fungi originated from healthy skin fruit is Trichoderma spp. around 35%. The in vitro test results of endophytic fungi antagonistic against P. palmivora indicated that the endophyte fungi originated from the leaf namely Aspergillus spp was obtained at 80 ± 2%, A. niger 90 ± 2%, A. flavus 100%, and Trichoderma spp. 90 ± 1.5%, the endophytic fungus originated from rods namely Neurospora spp. was 95 ± 2%, and Trichoderma spp. was 90 ± 2%. While the endophytic originated from rind namely Neurospora spp . was 95 ± 1.5 % and Trichoderma spp. was 80 ± 2%. The results of in vivo test of antagonistic endophytic fungi against P. palmivora showed that all of endophytic fungi (Aspergillus sp., A. niger, A. flavus, Neurospora sp., and Trichoderma sp.) have a significant effect in suppressing the growth of mycelium P. palmivora.Keywords: Endophytic Fungus, Phytophthora Palmivora, Inhibition, Prevalence, Biological Agents

Download Full-text

Aspernolides L and M, new butyrolactones from the endophytic fungus Aspergillus versicolor

Zeitschrift für Naturforschung C ◽

10.1515/znc-2016-0138 ◽

2017 ◽

Vol 72 (5-6) ◽

pp. 155-160 ◽

Cited By ~ 7

Author(s):

Sabrin R.M. Ibrahim ◽

Gamal A. Mohamed ◽

Samir A. Ross

Keyword(s):

Endophytic Fungi ◽

Endophytic Fungus ◽

Spectroscopic Analysis ◽

2D Nmr ◽

Systematic Search ◽

Aspergillus Versicolor ◽

Active Compounds ◽

Etoac Extract ◽

First Time

Abstract During the systematic search of active compounds from endophytic fungi, two new butyrolactones, namely aspernolides L (2) and M (4), together with four known compounds: 1-O-acetylglycerol (1), butyrolactone I (3), butyrolactone VI (5), and (+) alantrypinone (6) were characterized from the EtOAc extract of the endophytic fungus Aspergillus versicolor isolated from the roots of Pulicaria crispa (Asteraceae). Extensive spectroscopic analysis, including 1D, 2D NMR, and HRESIMS, was used to elucidate their structures. Compounds 1, 5, and 6 are reported for the first time from this fungus.

Download Full-text

Evaluasi Cendawan Endofit Asal Tanaman Karet untuk Mengendalikan Colletotrichum gloeosporioides Patogen Penyakit Gugur Daun Colletotrichum

Jurnal Tanaman Industri dan Penyegar ◽

10.21082/jtidp.v8n3.2021.p129-140 ◽

2021 ◽

Vol 8 (3) ◽

pp. 129

Author(s):

Rita Harni ◽

Khaerati Khaerati ◽

Edi Wardiana

Keyword(s):

Plant Growth ◽

Disease Severity ◽

Endophytic Fungi ◽

Endophytic Fungus ◽

Colletotrichum Gloeosporioides ◽

Leaf Fall ◽

Pathogen Colonization ◽

Crop Research ◽

Important Disease

Colletotrichum leaf fall disease caused by Colletotrichum gloeosporioides is an important disease in rubber plants. The C. gloeosporioides Infection can reduce production by 7%-45%. Controlling the pathogen using endophytic fungi is very promising because it can suppress inoculum and pathogen colonization, induce plant resistance, and trigger plant growth. The study aimed to evaluate the endophytic fungus isolate from rubber to control C. gloeosporioides as a pathogen that caused the rubber leaf fall disease. This research was carried out in the laboratory and greenhouse of the Indonesian Industrial and Beverage Crop Research Institute (IIBCRI), Sukabumi, from March to November 2018. The isolates used were endophytic fungi isolates from rubber plants, which were tested for their inhibition against C. gloeosporioides in vitro on rubber leaves and seedlings, and their mechanism. The variable observed were the inhibition rate, incubation periods, number of spots, disease severity, and plant growth. The results showed that the endophytic fungus could inhibit the growth of C. gloeosporioides about 64.17% - 86.67%. The high inhibitory activity (>80%) in isolates CEPR.19, CEPR.6, CEBPM.21, DTJE.1, and DMJE27 were 86.67%; 83.33%; 83.33%; 82.92%, and 82.50%, respectively. The observations on seedlings obtained three potential fungal isolates to control C. gloeosporioides on rubber leaves, namely CEBPM.21, CEPR19, and DTJE.1 with suppression of disease severity about 68.57%; 67.88%, and 60.20% with their mechanisms of action inducing resistance, antibiosis, competition, and hyperparasites.

Download Full-text

Alternative medium for the growth of endophytic fungi

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/886/1/012045 ◽

2021 ◽

Vol 886 (1) ◽

pp. 012045

Author(s):

Syamsia Syamsia ◽

Abubakar Idhan ◽

Husnah Latifah ◽

Noerfitryani Noerfityani ◽

Aidil Akbar

Keyword(s):

Organic Matter ◽

Research Methods ◽

Growth Medium ◽

Endophytic Fungi ◽

Endophytic Fungus ◽

Organic Medium ◽

Potato Dextrose Agar ◽

Organic Media ◽

Alternative Ingredients ◽

Agar Media

Abstract Medium for the growth of endophytic fungi generally uses Potato Dextrose Agar media, but because the price is expensive, it is necessary to find materials for alternative media from organic materials that are easy to obtain and inexpensive. The legume group was one of the alternative ingredients as the source of protein, corn, and rice as the source of carbohydrates for the growth medium. This study aimed to determine the potential of organic matter such as rice, corn, and legumes as a medium for the growth of endophytic fungi. The research methods included: rejuvenation of endophytic fungus isolation, preparation of organic medium from rice, corn, legumes, and potatoes, growth test of endophytic fungus on 4 types of organic media.

Download Full-text