scholarly journals Isolation of Endophytic Fungi QPS 05 from Quercus phillyraeoides A. Gray and Its Potential for α-Glucosidase Inhibitory Activity

2018 ◽  
Vol 20 (1) ◽  
pp. 1-7
Author(s):  
Anastasia Wheni Indrianingsih ◽  
Amalia Indah Prihantini ◽  
Sanro Tachibana
Keyword(s):  
Fatty Acids ◽  
Endophytic Fungi ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Life Cycles ◽  
Ic50 Values ◽  
Quercus Phillyraeoides ◽  
Alternaria Sp ◽  
Harmful Effects

AbstractEndophytic fungi are the microorganisms that spend all or part of their life cycles within plant tissue without causing harmful effects on the plant. In this study, 14 endophytic fungus from Quercus phillyraeoides A. Gray were isolated. Alternaria sp. QPS 05, an endophytic fungi which was isolated from the stem of Q. phillyraeoides A. Gray showed the highest α-glucosidase inhibitory activity. Further separation of ethyl acetate extract from the fungus led to the isolation of active substance from hexane-soluble fraction which give fatty acids mixture consist of palmitic acid, oleic acid, linoleic acid and linolenic acid (1) strong inhibitory activity against α-glucosidase. Isolated fatty acids (1) had inhibitory concentration (IC50) values against Saccharomyces cerevisiae was 12.10 μg/mL. The results of the present study showed that endophytic fungus from Alternaria sp. QPS 05 potentially contained a rich source of natural antidiabetic medicine.

Trichothecenes from an Endophytic Fungus Alternaria sp. sb23

Planta Medica ◽  
2020 ◽  
Vol 86 (13/14) ◽  
pp. 976-982
Author(s):  
Ying Gao ◽  
Jia Zhou ◽  
Hanli Ruan
Keyword(s):  
Endophytic Fungus ◽  
In Vitro Cytotoxicity ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Effects ◽  
Ic50 Values ◽  
Alternaria Sp ◽  
Optical Rotations ◽  
Ht 29 ◽  
Necrosis Factor

AbstractThree new (alterchothecenes A – C, 1 –3) and 3 known (4 –6) trichothecenes, along with 9 known compounds (7 –15), were isolated from the culture of Alternaria sp. sb23, an endophytic fungus separated from the root of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of 1–3 were determined through comparison of the experimental electronic circular dichroism (ECD) spectra and optical rotations with similar analogues. In vitro cytotoxicity tests of compounds 1–6 against human HT-29 colon carcinoma and human MCF-7 breast cancer cell lines indicated that 4–6 exhibited significant cytotoxic effects, with IC50 values ranging from 0.89 to 9.38 µM. And the potential of compounds 1–6 as tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) sensitizers in HT-29 cells was evaluated. The results revealed that combination treatment of TRAIL with compounds 1–6 synergistically decreased cell viability compared with the sole treatment with those compounds.

New Benzaldehyde and Benzopyran Compounds from the Endophytic Fungus Paraphaeosphaeria sp. F03 and Their Antimicrobial and Cytotoxic Activities

Planta Medica ◽  
2019 ◽  
Vol 85 (11/12) ◽  
pp. 957-964 ◽  
Author(s):  
Marcelo R. de Amorim ◽  
Felipe Hilário ◽  
Fernando M. dos Santos ◽  
João M. Batista ◽  
Tais M. Bauab ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
High Resolution Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Racemic Mixture ◽  
Cytotoxic Activities ◽  
Spectrometry Analysis ◽  
Ic50 Values ◽  
Benzaldehyde Derivatives ◽  
Mcf 7

AbstractThree new benzaldehyde derivatives, sporulosaldeins A – C (1–3), and 3 new benzopyran derivatives, sporulosaldeins D – F (4–6), were discovered from an endophytic fungus, Paraphaeosphaeria sp. F03, which was isolated from Paepalanthus planifolius leaves. Compounds 1–6 were elucidated by 1- and 2-dimensional nuclear magnetic resonance experiments and high-resolution mass spectrometry analysis. The absolute configuration of compound 5 was determined through the comparison of experimental and calculated electronic circular dichroism data. Compounds 1–6 were found to exhibit antifungal activity with minimum inhibitory concentration (MIC) values of 7.8 – 250 µg/mL and racemic mixture of compound 6 exhibited weak cytotoxicity against MCF-7 and LM3 with IC50 values of 34.4 and 39.2 µM, respectively.

scholarly journals α-Glucosidase Inhibitors: Diphenyl Ethers and Phenolic Bisabolane Sesquiterpenoids from the Mangrove Endophytic Fungus Aspergillus flavus QQSG-3

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 307 ◽  
Author(s):  
Yingnan Wu ◽  
Yan Chen ◽  
Xishan Huang ◽  
Yahong Pan ◽  
Zhaoming Liu ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Inhibitory Effects ◽  
Mangrove Endophytic Fungus ◽  
Glucosidase Inhibitors ◽  
Diphenyl Ethers ◽  
Ic50 Values ◽  
The Absolute

Two new diphenyl ethers (1 and 2) and four new phenolic bisabolane sesquiterpenoids (3–6), together with five known related derivatives, were isolated from the culture of the endophytic fungus Aspergillus flavus QQSG-3 obtained from a fresh branch of Kandelia obobata, which was collected from Huizhou city in the province of Guangdong, China. The structures of compounds 1–6 were determined by analyzing NMR and HRESIMS data. The absolute configurations of 5 and 6 were assigned by comparing their experimental ECD spectra with those reported for similar compounds in the literature. All isolates were evaluated for their α-glucosidase inhibitory activity, of which compounds 3, 5, 10, and 11 showed strong inhibitory effects with IC50 values in the range of 1.5–4.5 μM.

scholarly journals Hits-to-Lead Optimization of the Natural Compound 2,4,6-Trihydroxy-3-geranyl-acetophenone (tHGA) as a Potent LOX Inhibitor: Synthesis, Structure-Activity Relationship (SAR) Study, and Computational Assignment

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2509 ◽  
Author(s):  
Chean Ng ◽  
Kamal Rullah ◽  
Faridah Abas ◽  
Kok Lam ◽  
Intan Ismail ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Alkyl Chain ◽  
Kinetics Study ◽  
Toxicity Prediction ◽  
Structure Activity ◽  
Ic50 Value ◽  
Competitive Inhibitors ◽  
Ic50 Values ◽  
Sar Study

A new series of 2,4,6-trihydroxy-3-geranyl-acetophenone (tHGA) analogues were synthesized and evaluated for their lipoxygenase (LOX) inhibitory activity. Prenylated analogues 4a–g (half maximal inhibitory concentration (IC50) values ranging from 35 μ M to 95 μ M) did not exhibit better inhibitory activity than tHGA (3a) (IC50 value: 23.6 μ M) due to the reduction in hydrophobic interaction when the alkyl chain length was reduced. One geranylated analogue, 3d, with an IC50 value of 15.3 μ M, exhibited better LOX inhibitory activity when compared to tHGA (3a), which was in agreement with our previous findings. Kinetics study showed that the most active analogue (3e) and tHGA (3a) acted as competitive inhibitors. The combination of in silico approaches of molecular docking and molecular dynamic simulation revealed that the lipophilic nature of these analogues further enhanced the LOX inhibitory activity. Based on absorption, distribution, metabolism, excretion, and toxicity (ADMET) and toxicity prediction by komputer assisted technology (TOPKAT) analyses, all geranylated analogues (3a–g) showed no hepatotoxicity effect and were biodegradable, which indicated that they could be potentially safe drugs for treating inflammation.

scholarly journals Polyhydroxy p-Terphenyls from a Mangrove Endophytic Fungus Aspergillus candidus LDJ-5

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 82
Author(s):  
Guoliang Zhou ◽  
Xiaomin Zhang ◽  
Mudassir Shah ◽  
Qian Che ◽  
Guojian Zhang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Protein Tyrosine Phosphatase ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine Phosphatase 1B ◽  
Influenza Virus A ◽  
Protein Tyrosine ◽  
Ic50 Values ◽  
Aspergillus Candidus

Six undescribed polyhydroxy p-terphenyls, namely asperterphenyllins A–F, were isolated from an endophytic fungus Aspergillus candidus LDJ-5. Their structures were determined by NMR and MS data. Differing from the previously reported p-terphenyls, asperterphenyllin A represents the first p-terphenyl dimer connected by a C-C bond. Asperterphenyllin A displayed anti-influenza virus A (H1N1) activity and protein tyrosine phosphatase 1B (PTP1B) inhibitory activity with IC50 values of 53 μM and 21 μM, respectively. The anti-influenza virus A (H1N1) activity and protein tyrosine phosphatase 1B (PTP1B) inhibitory activity of p-terphenyls are reported for the first time. Asperterphenyllin G exhibited cytotoxicity against nine cell lines with IC50 values ranging from 0.4 to 1.7 μM. Asperterphenyllin C showed antimicrobial activity against Proteus species with a MIC value of 19 μg/mL.

scholarly journals Polyketide Derivatives, Guhypoxylonols A–D from a Mangrove Endophytic Fungus Aspergillus sp. GXNU-Y45 That Inhibit Nitric Oxide Production

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 5
Author(s):  
Xiaoya Qin ◽  
Jiguo Huang ◽  
Dexiong Zhou ◽  
Wenxiu Zhang ◽  
Yanjun Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Inflammatory Activity ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Mangrove Endophytic Fungus ◽  
Ic50 Values ◽  
Aspergillus Sp

Four undescribed compounds, guhypoxylonols A (1), B (2), C (3), and D (4), were isolated from the mangrove endophytic fungus Aspergillus sp. GXNU-Y45, together with seven previously reported metabolites. The structures of 1–4 were elucidated based on analysis of HRESIMS and NMR spectroscopic data. The absolute configurations of the stereogenic carbons in 1–3 were established through a combination of spectroscopic data and electronic circular dichroism (ECD). Compounds 1–11 were evaluated for their anti-inflammatory activity. Compounds 1, 3, 4, and 6 showed an inhibitory activity against the production of nitric oxide (NO), with the IC50 values of 14.42 ± 0.11, 18.03 ± 0.14, 16.66 ± 0.21, and 21.05 ± 0.13 μM, respectively.

scholarly journals Characterization of Maltase and Sucrase Inhibitory Constituents from Rhodiola crenulata

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 540 ◽  
Author(s):  
Wen-Tai Li ◽  
Yu-Hsuan Chuang ◽  
Jung-Feng Hsieh
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Epicatechin Gallate ◽  
Competitive Inhibitors ◽  
Inhibition Kinetic ◽  
Ic50 Values ◽  
Rhodiola Crenulata ◽  
Inhibition Constants

The inhibitory properties of epicatechin-(4β,8)-epicatechingallate (B2-3’-O-gallate), epicatechin gallate (ECG), and epicatechin (EC) isolated from Rhodiola crenulata toward maltase and sucrase were investigated. The half-maximal inhibitory concentration (IC50) values for maltase were as follows: B2-3’-O-gallate (1.73 ± 1.37 μM), ECG (3.64 ± 2.99 μM), and EC (6.25 ± 1.84 μM). Inhibition kinetic assays revealed the inhibition constants (Ki) of the mixed-competitive inhibitors of maltase, as follows: B2-3’-O-gallate (1.99 ± 0.02 μM), ECG (3.14 ± 0.04 μM), and EC (7.02 ± 0.26 μM). These compounds also showed a strong inhibitory activity toward sucrase, and the IC50 values of B2-3’-O-gallate, ECG, and EC were 6.91 ± 3.41, 18.27 ± 3.99, and 18.91 ± 3.66 μM, respectively. Inhibition kinetic assays revealed the inhibition constants (Ki) of the mixed-competitive inhibitors of sucrase as follows: B2-3’-O-gallate (6.05 ± 0.04 μM), ECG (8.58 ± 0.08 μM), and EC (13.72 ± 0.15 μM). Overall, these results suggest that B2-3’-O-gallate, ECG, and EC are potent maltase and sucrase inhibitors.

scholarly journals Daldiniaeschsone A, a Rare Tricyclic Polyketide Having a Chromone Unit Fused to a δ-Lactone and Its Symmetrical Biphenyl Dimer, Daldiniaeschsone B, from an Endophytic Fungus Daldinia eschscholtzii SDBR-CMUNKC745

2021 ◽  
Vol 7 (5) ◽  
pp. 358
Author(s):  
Natnicha Wutthiwong ◽  
Virayu Suthiphasilp ◽  
Aknarin Pintatum ◽  
Nakarin Suwannarach ◽  
Jaturong Kumla ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Ic50 Values

Daldiniaeschsone A (1), a rare tricyclic polyketide having a chromone unit fused to a δ-lactone and its symmetrical 6,6'-biphenyl dimer, daldiniaeschsone B (2), together with three known compounds (3-5), were isolated from a plant-derived endophytic fungus, Daldinia eschscholtzii SDBR-CMUNKC745. Their structures were elucidated by extensive 1D and 2D NMR spectroscopic data and HRESIMS. All compounds showed α-glucosidase inhibitory activity with IC50 values ranging from 0.16-0.85 mM and compound 1 was the best α-glucosidase inhibitory activity (IC50 = 0.16 mM).

scholarly journals Novel Polyketides Produced by the Endophytic Fungus Aspergillus Fumigatus from Cordyceps Sinensis

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1709 ◽  
Author(s):  
Da-Le Guo ◽  
Xiao-Hua Li ◽  
Dan Feng ◽  
Meng-Ying Jin ◽  
Yu-Mei Cao ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
2D Nmr ◽  
Cordyceps Sinensis ◽  
Two Dimensional ◽  
One Dimensional ◽  
Ic50 Values

Five new polyketides, including two pairs of enantiomers and a racemate, were isolated from the fermentation broth of Aspergillus fumigatus, an endophytic fungus isolated from Cordyceps sinensis. Their structures were identified using one-dimensional (1D) and two-dimensional (2D) NMR experiments, and the absolute configurations of the enantiomers were confirmed using electronic circular dichroism (ECD) calculations. Compounds 1a and 2a exhibited inhibitory activity against the MV4-11 cell line in vitro, with IC50 values of 23.95 µM and 32.70 µM, respectively.

Research on the Inhibitory Activity of Endophytic Fungi in Huperzia serrata against AchE

2013 ◽  
Vol 303-306 ◽  
pp. 2598-2601
Author(s):  
Na Li ◽  
Zi Ping Zhu
Keyword(s):  
Endophytic Fungi ◽  
Active Ingredient ◽  
Inhibitory Activity ◽  
Bacterial Strain ◽  
Total Alkaloid ◽  
Inhibitory Concentration ◽  
Fermentation Broth ◽  
Inhibition Activity ◽  
Fermentation Products ◽  
Huperzia Serrata

Twenty-two endophytic fungi in the Huperzia serrata were separated to test their fermentation broth. Research found that seven of them could produce alkaloid. Of these, the fermentation products from the T4 bacterial strain produced the most, with 2.027 mg/g. Ellman colorimetry has determined that the fermentation broth in four fungi had inhibition activity against AchE. It also showed that the total alkaloid of the T17 bacterial strain had a half-inhibitory concentration (IC50) of 98.494 μg•mL-1 on the mouse’s brain against AchE. This yielded the conclusion that the total alkaloid in the T17 fermentation broth restrains AchE effectively, and that it further contributes to the separation of its active ingredient.

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