Simultaneous Quantification of a Herbal Combination of Pueraria lobata, Salvia miltiorrhiza and Panax notoginseng by Rapid Resolution Liquid Chromatography

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200
Author(s):  
Shu-Chao Yan ◽  
Jie Ma ◽  
Qian-Qian Huang ◽  
Ana Mani ◽  
Ya-ling Cai ◽  
...  
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
Salvia Miltiorrhiza ◽  
Panax Notoginseng ◽  
Traditional Chinese Medicines ◽  
Pueraria Lobata ◽  
Chinese Medicines ◽  
Rapid Resolution ◽  
Simultaneous Quantification

Pueraria lobata, Salvia miltiorrhiza and Panax notoginseng are traditional Chinese medicines which have been used for thousands of years either singly or in combination. However, the quality of formulas containing either all or part of the three herbs is, at present, controlled by only one or two easily detectable compounds.. A rapid resolution liquid chromatography (RRLC) method has been developed for the quality control of the three herbs in combination through ten active ingredients originating from the raw herbs. The analysis was achieved in a run of less than 25 minutes. Using the same method, the finger-prints of the three herbs are detectable in the chromatogram, and so the method could be applied for identification of different species in the same genus. Four formula preparations {xinkeshu capsule (XKSC), xinkeshupian (XKSP), fufang-danshenpian (FFDSP), and naodeshengpian (NDSP)} were analyzed by this new RRLC method. Controlling only one or two easily detectable ingredients is far from enough to assure the quality of the formula preparations. However, controlling multiple ingredients originating from the raw herbs and their ratios is an effective and feasible approach to solve this problem.

scholarly journals Simultaneous Quantification of Panax and Epimedium Species Using Rapid Resolution Liquid Chromatography (RRLC)

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Jie Ma ◽  
Yuan-Chun Ma ◽  
Daniel Wang ◽  
Fei Fei Hou ◽  
Mai Luo ◽  
...  
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
Pure Water ◽  
Complex Mixtures ◽  
Rapid Resolution ◽  
Simultaneous Quantification ◽  
Panax Species ◽  
Reference Samples

Two Rapid Resolution Liquid Chromatography (RRLC) methods have been developed and validated for simultaneous quantification of eight major ginsenosides from Panax species, namely, R1, Rg1, Re, Rf, Rb1, Rb2, Rc, and Rd, and flavonoids from Epimedium species, namely, epimedins A, B, and C and icariin. The analyses were performed using an Agilent 1200 series RRLC system with Phenomenex Luna C18-HST and Zorbax Eclipse XDB columns. The separation was performed with a gradient mobile phase of A (pure water) and B (acetonitrile) at a flow rate of 1.0 mL/ min and 2.5 mL/min, respectively. Both columns were kept at 40°C with the detection wavelength set at 203 nm. Specific eluted compounds were identified by using reference samples of ginsenosides R1, Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd, and epimedins A, B, C and icariin. Baseline separation was achieved in less than 15 minutes for the Phenomenex Luna column and 4 minutes for the Zorbax Eclipse column. Characteristic RRLC profiles were established for complex mixtures of ginsenosides from Panax species and flavonoids from Epimedium species. Both methods developed here are effective for the quality control of formulated products containing both Panax and Epimedium varieties.

Characteristic Chromatogram: A Method of Discriminate and Quantitative Analysis for Quality Evaluation of Uncaria Stem with Hooks

Planta Medica ◽  
2017 ◽  
Vol 84 (06/07) ◽  
pp. 449-456 ◽  
Author(s):  
Jinjun Hou ◽  
Ruihong Feng ◽  
Yibei Zhang ◽  
Huiqin Pan ◽  
Shuai Yao ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Indole Alkaloids ◽  
Total Content ◽  
Traditional Chinese Medicines ◽  
Herbal Drugs ◽  
Chinese Medicines ◽  
Total Analysis Time ◽  
Total Analysis ◽  
Single Standard

AbstractIt remains a challenge to establish new monographs for herbal drugs derived from multiple botanical sources. Specifically, the difficulty involves discriminating and quantifying these herbs with components whose levels vary markedly among different samples. Using Uncaria stem with hooks as an example, a characteristic chromatogram was proposed to discriminate its five botanical origins and to quantify its characteristic components in the chromatogram. The characteristic chromatogram with respect to the components of Uncaria stem with hooks with the five botanical origins was established using 0.02% diethylamine and acetonitrile as the mobile phase. The total analysis time was 50 min and the detection wavelength was 245 nm. Using the same chromatogram parameters, the single standard to determine multicomponents method was validated to simultaneously quantify nine indole alkaloids, including vincosamide, 3α-dihydrocadambine, isocorynoxeine, corynoxeine, isorhynchophylline, rhynchophylline, hirsuteine, hirsutine, and geissoschizine methyl ether. The results showed that only the Uncaria stem with hooks from Uncaria rhynchophylla, the most widely used in the herbal market, showed the presence of these nine alkaloids. The conversion factors were 1.27, 2.32, 0.98, 1.04, 1.00, 1.02, 1.26, 1.33, and 1.25, respectively. The limits of quantitation were lower than 700 ng/mL. The total contents of 31 batches of Uncaria stem with hooks were in the range of 0.1 – 0.6%, except for Uncaria hirsuta Havil and Uncaria sinensis (Oliv.) Havil. The results also showed that the total content of indole alkaloids tended to decrease with an increase in the hook diameter. This showed that the characteristic chromatogram is practical for controlling the quality of traditional Chinese medicines with multiple botanical origins.

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