Exploring the Insect Control and Plant Growth Promotion Potentials of Endophytes Isolated From Calotropis procera Present in Jeddah KSA

Pseudomonas bacteria are entomopathogenic that can naturally infect and kill insects upon ingestion. The insecticidal and plant growth-promoting roles of the bacteria were assessed by applying Pseudomonas IUK001 to insects and plants. The culture extract (CE) of IUK001 at the concentration of 1 mL/cm3 of an artificial diet was used as a treatment for Galleria mellonella larvae. The CE was heat stable at 70°C for 30 minutes and proteinase-K stable, showing 100% and 90% insecticidal activity against G. mellonella larvae. Gas chromatography–mass spectrometry (GC–MS) analysis revealed the insecticidal compounds, for example, trans-cinnamic acid, ornithine, and cyclo (l-Pro-l-Val) in the CE of IUK001. Antibacterial activity was assessed through minimal inhibitory concentration and half-maximal inhibitory concentration values, which ranged from 28 ± 0.8 and 18.9 ± 0.7 to 8 ± 1.8 and 4.6 ± 0.6, respectively. Moreover, plants were inoculated with IUK001, which significantly enhanced ( P < 0.05) the total plant lengths (root + shoot), dry and fresh biomasses and chlorophyll content, and also induced lateral roots. Plant growth hormones, auxins: indole-3-butyric acid and indole-3- propionic acid, were then analyzed through GC–MS in the CE using their respective isotopic internal standards. The findings of the study suggest that IUK001 is a significantly valuable candidate to be exploited as a biocontrol agent as well as a plant growth promoter.

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Variovorax sp. Has an Optimum Cell Density to Fully Function as a Plant Growth Promoter

Microorganisms ◽

10.3390/microorganisms7030082 ◽

2019 ◽

Vol 7 (3) ◽

pp. 82 ◽

Cited By ~ 1

Author(s):

Oyungerel Natsagdorj ◽

Hisayo Sakamoto ◽

Dennis Santiago ◽

Christine Santiago ◽

Yoshitake Orikasa ◽

...

Keyword(s):

Plant Growth ◽

Cell Density ◽

Growth Promotion ◽

Growth Promoter ◽

Plant Growth Promoting Bacteria ◽

Biochemical Tests ◽

Green Pepper ◽

Plant Growth Promoter ◽

Plant Growth Promoting ◽

Growth Promoting

Utilization of plant growth-promoting bacteria colonizing roots is environmentally friendly technology instead of using chemicals in agriculture, and understanding of the effects of their colonization modes in promoting plant growth is important for sustainable agriculture. We herein screened the six potential plant growth-promoting bacteria isolated from Beta vulgaris L. (Rhizobium sp. HRRK 005, Polaromonas sp. HRRK 103, Variovorax sp. HRRK 170, Mesorhizobium sp. HRRK 190, Streptomyces sp. HRTK 192, and Novosphingobium sp. HRRK 193) using a series of biochemical tests. Among all strains screened, HRRK 170 had the highest potential for plant growth promotion, given its ability to produce plant growth substances and enzymes such as siderophores and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, respectively, concomitantly with active growth in a wider range of temperatures (10–30 °C) and pH (5.0–10.0). HRRK 170 colonized either as spots or widely on the root surface of all vegetable seedlings tested, but significant growth promotion occurred only in two vegetables (Chinese cabbage and green pepper) within a certain cell density range localized in the plant roots. The results indicate that HRRK 170 could function as a plant growth promoter, but has an optimum cell density for efficient use.

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Survival and colonization of rhizobacteria in a tomato transplant system

Canadian Journal of Microbiology ◽

10.1139/w03-051 ◽

2003 ◽

Vol 49 (6) ◽

pp. 383-389 ◽

Cited By ~ 44

Author(s):

Zhinong Yan ◽

M S Reddy ◽

Joseph W Kloepper

Keyword(s):

Plant Growth ◽

Pseudomonas Fluorescens ◽

Seed Treatment ◽

Bacillus Pumilus ◽

Growth Promotion ◽

Lateral Roots ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Colonization ◽

Initial Inoculum ◽

Application Method

Plant-growth-promoting rhizobacteria (PGPR) are used on crops most often as seed treatments; however, an alternative application method for transplanted vegetables is mixing PGPR into the soilless medium in which the transplants are grown. Studies were undertaken to compare root colonization and persistence of rifampicin-resistant mutants of PGPR strains Bacillus pumilus SE34 and Pseudomonas fluorescens 89B61, SE34r and 89B61r, on tomato as a function of application method. When the bacteria were incorporated into Promix(tm) soilless medium at log 6, 7, and 8 colony- forming units/g, populations of strain SE34r per gram of medium maintained the initial inoculum densities, while populations of 89B61r decreased approximately one to two orders of magnitude by 4 weeks after planting. The populations of each PGPR strain colonizing roots after application into the soilless medium showed a similar pattern at 6 weeks as that at 4 weeks after planting, with higher populations on the whole roots and lateral roots than on the taproots. Strain SE34r but not 89B61r moved upwards and colonized the phyllosphere when incorporated into the soilless medium. Following application as seed treatment, populations of SE34r were significantly higher on upper roots and on the taproot than were populations following application through the soilless medium. Conversely, populations were higher on lower roots and lateral roots following application through the soilless medium than were populations following application as seed treatment. While strain SE34 enhanced plant growth with application both to the medium and as seed treatment, the level of growth promotion was significantly greater with application in the soilless medium. The results indicate that PGPR can be successfully incorporated into soilless media in vegetable transplant production systems.Key words: rhizobacteria, plant colonization, Bacillus pumilus, Pseudomonas fluorescens.

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Colonization and persistence of a plant growth-promoting bacterium Pseudomonas fluorescens strain CS85, on roots of cotton seedlings

Canadian Journal of Microbiology ◽

10.1139/w04-040 ◽

2004 ◽

Vol 50 (7) ◽

pp. 475-481 ◽

Cited By ~ 12

Author(s):

Chunxia Wang ◽

Daoben Wang ◽

Qi Zhou

Keyword(s):

Plant Growth ◽

Pseudomonas Fluorescens ◽

Growth Promotion ◽

Lateral Roots ◽

Marker Genes ◽

Original Strain ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Root Surfaces

Pseudomonas fluorescens CS85, which was previously isolated from the rhizosphere of cotton seedlings, acts as both a plant growth-promoting bacterium and a biocontrol agent against cotton pathogens, including Rhizoctonia solani, Colletotrichum gossypii, Fusarium oxysporum f sp. vasinfectum, and Verticillium dahliae. Strain CS85 was labeled separately with luxAB and gusA. The labeled strains were stably maintained and had high levels of expression of the marker genes, luxAB and gusA, after successive transfers on nonselective medium, long-term preservation, and after recovery from soil. The labeled strains displayed similar biocontrol characteristics (e.g., antibiosis, effects of growth -promotion and disease -control) to the original strain. The labeled strains colonized all surfaces of the young plant root zones, such as roots hairs and lateral roots, although the distribution of the labeled strains on the root surfaces was not uniform. Moreover, the population densities of the labeled strains on the root surface were stably maintained at high levels during the first 2 weeks of plant growth in the native soil, so that about 107–108 CFU/g root were detected, then decreased gradually. Nevertheless, approximately 106 CFU/g root of the labeled strains were observed on the root surfaces 35 d after planting.Key words: plant growth-promoting bacteria, luxAB, gusA, root colonization.

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Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200916 ◽

2019 ◽

Vol 20 (9) ◽

Cited By ~ 1

Author(s):

Aris Tri Wahyudi ◽

J.A. Priyanto ◽

HANIFA NUR Fijrina ◽

HIMA DEWI Mariastuti ◽

ABDJAD ASIH Nawangsih

Keyword(s):

Plant Growth ◽

Rhizosphere Soil ◽

Lateral Roots ◽

Stem Length ◽

Growth Promoter ◽

Free Medium ◽

Maize Rhizosphere ◽

Plant Growth Promoter ◽

Streptomyces Spp ◽

Sprout Growth

Abstract. Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas 20: 2547-2553. Actinomycete is one group of rhizobacteria that plays an important role as a plant growth promoter. This study was aimed to evaluate the potential of Actinomycetes isolated from maize rhizosphere in promoting plant growth in vitro including their ability to produce IAA, promote maize sprout growth, solubilize phosphate, and grow in N-free medium. Thirty isolates have been isolated from maize rhizosphere using a spread plate method. All 30 isolates were probably not pathogenic to plants as tested by hypersensitivity reaction test on tobacco leaves. Based on the colorimetric assay, 30 isolates (100%) were able to produce IAA with concentrations ranging from 1.05 to 26.89 ppm. The highest concentration of IAA (26.89 ppm) produced by ARJ 21 and the lowest IAA concentration (1.05 ppm) produced by ARJ 12. By using the Ragdoll method, it showed that 9 isolates (30%) were able to promote maize sprout growth significantly on five growth parameters including primary root length, stem length, number of lateral roots, wet weight and dry weight. Twenty-one isolates (70%) were capable of solubilizing phosphate in Pikovskaya medium containing tricalcium phosphate. Also, 30 isolates (100%) were able to grow on N-free medium, suggesting their ability to fix nitrogen. Based on 16S-rRNA, five potential isolates with plant growth-promoting properties were highly similar to Streptomyces spp. Based on their potential characters, these Actinomycetes isolates have the potential to be further developed as a biofertilizer agent for sustainable maize farming.

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Comparative Study on Plant Growth Promotion by Endophytic Pseudomonas Spp. and Bacillus Spp. of Solanum Lycopersicum

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v4i4.16251 ◽

2017 ◽

Vol 4 (4) ◽

pp. 464-469

Author(s):

Sampada Mishra ◽

Saroj Kumar Mahato ◽

Sabin Basi ◽

Shradha Basi-Chipalu

Keyword(s):

Plant Growth ◽

Solanum Lycopersicum ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Shoot Biomass ◽

Shoot Height ◽

Plant Growth Hormones ◽

Pseudomonas Spp ◽

Bacillus Spp ◽

Phosphate Solubilizing

Minimization of deleterious effects of chemical fertilizers on health, ecosystem and economy can only be achieved by finding healthy, eco-friendly and cheap alternatives. Naturally selected symbiotic relationship between the endophytic bacteria and their host plants makes them an ideal candidate as biofertilizer. They can synthesize various plant growth hormones as well as assist their host in uptake of nutrients from soil.The study was designed to compare plant growth promotion of Solanum lycopersicum by Bacillus spp., Pseudomonas spp. and total endophytic community isolated from roots of S. lycopersicum, grown in the soil samples collected from various locations of Kathmandu valley of Nepal. Tomato seeds were inoculated with mixtures of eight endophytic strains of Bacillus spp. and Pseudomonas spp., and crude endophytes obtained from each location separately.Endophytic treatments, except Pseudomonas spp., inhibited seminal root growth during 12-days germination period. However, after plantation, root and shoot biomass was enhanced by the endophytes, with no significant differences among the bacterial treatments. The shoot height was also enhanced, among which Pseudomonas spp. had the strongest effect. In phosphate solubilization assay, out of seventy-two isolates each of Bacillus spp. and Pseudomonas spp. tested, twenty-four isolates of Pseudomonas spp. and sixteen isolates of Bacillus spp. could solubilize phosphate. Higher number of phosphate solubilizing isolates of Pseudomonas spp. might provide a possible explanation for the greater enhancement of shoot height by Pseudomonas spp. as compared to Bacillus spp.Int J Appl Sci Biotechnol, Vol 4(4): 464-469

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Growth promoters in banana seedlings submitted to saline irrigation

Revista Engenharia na Agricultura - REVENG ◽

10.13083/reveng.v29i1.12500 ◽

2021 ◽

Vol 29 ◽

pp. 315-324

Author(s):

Daniela Andreska da Silva ◽

Ricardo Leoni Gonçalves Bastos ◽

Christiana de Fátima Bruce da Silva ◽

Alan Bernard Oliveira de Sousa

Keyword(s):

Plant Growth ◽

Leaf Area ◽

Plant Height ◽

Irrigation Water ◽

Root Length ◽

Growth Promotion ◽

Growth Promoter ◽

Saline Irrigation ◽

Bacillus Spp ◽

Number Of Leaves

The objective of this study was to evaluate the effect of inoculation of a plant growth promoter bacteria on the growth of micropropagated banana seedlings cultivar Williams under irrigation with water at different saline levels. The experiment was carried out in a greenhouse at Embrapa Agroindústria Tropical, Fortaleza, State of Ceará. The experimental design was in randomized blocks, in a 3 x 4 factorial scheme, corresponding to the three factors for growth promotion (negative control: water; Osmocote® slow-release fertilizer and a Bacillus spp. bacterium) subjected to four levels of irrigation water salinity (S1 = 0.5; S2 = 1.5; S3 = 3.0 and S4 = 4.5 dS m-1), and five blocks, totaling 60 experimental units. Sixty days after transplanting (DAT) and application of treatments, the following variables related to plant growth were measured: number of leaves (NL), pseudostem diameter (PD), plant height (PH), leaf area (LA), and root length (RL). The rise in saline levels in the irrigation water negatively influenced the variables number of leaves, pseudostem diameter, root length, and leaf area, showing a decreasing linear behavior. The variables number of leaves and leaf area of seedlings inoculated in association with Bacillus spp. did not differ from each other, regardless of the saline level. This indicated a likely increase in the response to the salinity tolerance of the seedlings. Treatment with Osmocote® fertilizer differed statistically for variables plant height, pseudostem diameter, and leaf area.

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Draft Genome Sequence of Bacillus altitudinis Lc5, a Biocontrol and Plant Growth-Promoting Endophyte Strain Isolated from Indigenous Black Rice of Manipur

Genome Announcements ◽

10.1128/genomea.00601-18 ◽

2018 ◽

Vol 6 (26) ◽

Cited By ~ 4

Author(s):

Momota Potshangbam ◽

Dinabandhu Sahoo ◽

Preveen Verma ◽

Sandhya Verma ◽

Mohan Chandra Kalita ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Draft Genome ◽

Growth Promoter ◽

Black Rice ◽

Content Type ◽

Bacillus Altitudinis ◽

Genome Study ◽

Plant Growth Promoting ◽

Growth Promoting

We report here the 3.6-Mb draft genome of Bacillus altitudinis Lc5, a potential plant growth promoter and an active antagonistic endophyte of black rice. This genome study will provide better insights into the strain’s mechanisms for plant growth promotion and biocontrol, thus facilitating its application in organic agriculture.

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Mycogenic Nano-Complex for Plant Growth Promotion and Bio-Control of Pythium aphanidermatum

Plants ◽

10.3390/plants10091858 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1858

Author(s):

Shaima M.N. Moustafa ◽

Rania H. Taha

Keyword(s):

Plant Growth ◽

Metal Complex ◽

Vicia Faba ◽

Growth Promotion ◽

Pathogenic Fungus ◽

Pythium Aphanidermatum ◽

Root Weight ◽

Total Biomass ◽

Growth Promoter ◽

Green Method

(1) Background: biological way is one of the most ecofriendly and safe strategies for nanomaterials synthesis. So, biosynthesis-green method was used for the preparation of Zn(II) complex (in the Nano scale) from the reaction of the schiff base ligand 2,2′-((1E,1′E)-(1,2-phenylenebis (azanylylidene)), bis(methanylylidene))bis(4-bromophenol), and Zn(II)sulphate. The biogenic ZnNP-T was characterized by different methods. Our purpose was to evaluate the ability of biosynthesis-green method for the preparation of Zn(II) complex as an antifungal agent against diseases from fungal species. (2) Methods: in this work, isolates of Pythium aphanidermatum and Trichderma harzianum were obtained, and Trichderma harzianum was used to prepare nano metal complex. We tested the pathogenicity of nano metal complex against seedling and germination of seeds, and we evaluated the effectiveness of ZnNP-T for growth promotion of Vicia feba in early stage and inhibitory activity against Pythium aphanidermatum. (3) Results: antagonistic activity of ZnNP-T was tested in vitro against Pythium aphanidermatum, and then the growth rates of Vicia faba were determined. The obtained data revealed that mycelial growth of pathogenic fungus was inhibited about 73.8% at 20 ppm. In addition, improved the total biomass of Vicia faba in the presence of P. aphanidermatum. All concentration of ZnNP-T positively affected root weight of Vicia faba seedlings, and positively affected shoot weight. Root and shoot lengths were affected by using 20 ppm of ZnNP-T with up to 180 and 96.5 mm of shoot and root length compared to that of the control, while germination percentage was significantly enhanced with up to 100% increase after 72 h of germination. (4) Conclusion: one of the modern challenges in vegetable or fruit production is to enhance seed germination and to grow healthy plants with strong root system. In future, there should be a focus on using of biogenic Zinc nano-complex as plant growth promoter agents.

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Exploration of Klebsiella pneumoniae M6 for paclobutrazol degradation, plant growth attributes, and biocontrol action under subtropical ecosystem

PLoS ONE ◽

10.1371/journal.pone.0261338 ◽

2021 ◽

Vol 16 (12) ◽

pp. e0261338

Author(s):

Govind Kumar ◽

Shatrohan Lal ◽

Shailendra K. Maurya ◽

A. K. Bhattacherjee ◽

Parul Chaudhary ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Plant Growth ◽

Growth Promotion ◽

Degradation Kinetics ◽

Principal Component ◽

Pathogenic Fungi ◽

Inoculum Size ◽

Growth Promoter ◽

Degraded Land ◽

Soil System

In recent times, injudicious use of paclobutrazol (PBZ) in mango orchards deteriorates the soil quality and fertility by persistence nature and causes a serious ecosystem imbalance. In this study, a new Klebsiella pneumoniae strain M6 (MW228061) was isolated from mango rhizosphere and characterized as a potent plant growth promoter, biocontrol, and PBZ degrading agent. The strain M6 efficiently utilizes PBZ as carbon, energy and nitrogen source and degrades up to 98.28% (50 mgL-1 initial conc.) of PBZ at 15th day of incubation in MS medium. In the soil system first order degradation kinetics and linear model suggested 4.5 days was the theoretical half-life (t1/2 value) of PBZ with strain M6. Box Behnken design (BBD) model of Response surface methodology (RSM) showed pH 7.0, 31°C temperature, and 2.0 ml inoculum size (8 x 109 CFU mL-1) was optimized condition for maximum PBZ degradation with strain M6. Plant growth promoting attributes such as Zn, K, PO4 solubilization IAA, HCN and NH3 production of strain M6 showed positive results and were assessed quantitatively. The relation between plant growth promotion and PBZ degradation was analyzed by heat map, principal component analysis (PCA) and, clustal correlation analysis (CCA). Strain M6 was also showing a significant biocontrol activity against pathogenic fungi such as Fusarium oxysporum (MTCC–284), Colletotrichum gloeosporioides (MTCC– 2190), Pythium aphanidermatum (MTCC– 1024), Tropical race 1 (TR -1), and Tropical race 4 (TR -4). Hence, results of the study suggested that strain M6 can be utilized as an effective bio-agent to restore degraded land affected by persistent use of paclobutrazol.

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What do patents tell us about microalgae in agriculture?

AMB Express ◽

10.1186/s13568-021-01315-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Mayara Mari Murata ◽

Luiz Rodrigo Ito Morioka ◽

Josemeyre Bonifacio Da Silva Marques ◽

Alessandra Bosso ◽

Hélio Hiroshi Suguimoto

Keyword(s):

Plant Growth ◽

Agricultural Sector ◽

Growth Promotion ◽

The United States ◽

World Intellectual Property Organization ◽

European Patent ◽

Growth Promoter ◽

Industrial Property ◽

Alternative Source ◽

Patent Documents

AbstractMicroalgae have been used widely as a biological source for several industries, such as biofuel, pharmaceutical and food. Recently, the agricultural industry has also began using microalgae as an alternative source for sustainable products to replace agrochemicals. Due to the lack of scientific articles in this research area, the objective of this study was to search for applications of microalgae and to characterize its use in agriculture using the patent documents available in three patent databases, World Intellectual Property Organization (WIPO), European Patent Office (EPO) and Brazilian Institute of Industrial Property (INPI). The search was carried out using the keyword “microalgae” and applying the filter for International Patent Classification (IPC) code “A01N” which corresponds to patents related to agriculture and cultivation of microalgae. Our patent database search returned 669 documents and 132 patents were selected for the study based on their abstracts. The first patent was registered in 1982 and described the use of microalgae Chlorella extract as a plant growth promoter. After that, no patent was registered for 15 years. From 2005 to 2014, only seven patents were found. However, the scenario changed from 2015 when the number of patents increased mainly in the United States, China and Europe. The patent analysis showed several applications for microalgae in the agricultural sector, such as plant growth promotion, biofertilization, plant disease control, weed management, and post-harvest quality. This review confirmed the increasing interest in microalgae-derived products in agriculture and the value of using patent documents to assess innovative areas.

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