scholarly journals Exploration of Klebsiella pneumoniae M6 for paclobutrazol degradation, plant growth attributes, and biocontrol action under subtropical ecosystem

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261338
Author(s):  
Govind Kumar ◽  
Shatrohan Lal ◽  
Shailendra K. Maurya ◽  
A. K. Bhattacherjee ◽  
Parul Chaudhary ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Plant Growth ◽  
Growth Promotion ◽  
Degradation Kinetics ◽  
Principal Component ◽  
Pathogenic Fungi ◽  
Inoculum Size ◽  
Growth Promoter ◽  
Degraded Land ◽  
Soil System

In recent times, injudicious use of paclobutrazol (PBZ) in mango orchards deteriorates the soil quality and fertility by persistence nature and causes a serious ecosystem imbalance. In this study, a new Klebsiella pneumoniae strain M6 (MW228061) was isolated from mango rhizosphere and characterized as a potent plant growth promoter, biocontrol, and PBZ degrading agent. The strain M6 efficiently utilizes PBZ as carbon, energy and nitrogen source and degrades up to 98.28% (50 mgL-1 initial conc.) of PBZ at 15th day of incubation in MS medium. In the soil system first order degradation kinetics and linear model suggested 4.5 days was the theoretical half-life (t1/2 value) of PBZ with strain M6. Box Behnken design (BBD) model of Response surface methodology (RSM) showed pH 7.0, 31°C temperature, and 2.0 ml inoculum size (8 x 109 CFU mL-1) was optimized condition for maximum PBZ degradation with strain M6. Plant growth promoting attributes such as Zn, K, PO4 solubilization IAA, HCN and NH3 production of strain M6 showed positive results and were assessed quantitatively. The relation between plant growth promotion and PBZ degradation was analyzed by heat map, principal component analysis (PCA) and, clustal correlation analysis (CCA). Strain M6 was also showing a significant biocontrol activity against pathogenic fungi such as Fusarium oxysporum (MTCC–284), Colletotrichum gloeosporioides (MTCC– 2190), Pythium aphanidermatum (MTCC– 1024), Tropical race 1 (TR -1), and Tropical race 4 (TR -4). Hence, results of the study suggested that strain M6 can be utilized as an effective bio-agent to restore degraded land affected by persistent use of paclobutrazol.

scholarly journals Variovorax sp. Has an Optimum Cell Density to Fully Function as a Plant Growth Promoter

2019 ◽  
Vol 7 (3) ◽  
pp. 82 ◽  
Author(s):  
Oyungerel Natsagdorj ◽  
Hisayo Sakamoto ◽  
Dennis Santiago ◽  
Christine Santiago ◽  
Yoshitake Orikasa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Cell Density ◽  
Growth Promotion ◽  
Growth Promoter ◽  
Plant Growth Promoting Bacteria ◽  
Biochemical Tests ◽  
Green Pepper ◽  
Plant Growth Promoter ◽  
Plant Growth Promoting ◽  
Growth Promoting

Utilization of plant growth-promoting bacteria colonizing roots is environmentally friendly technology instead of using chemicals in agriculture, and understanding of the effects of their colonization modes in promoting plant growth is important for sustainable agriculture. We herein screened the six potential plant growth-promoting bacteria isolated from Beta vulgaris L. (Rhizobium sp. HRRK 005, Polaromonas sp. HRRK 103, Variovorax sp. HRRK 170, Mesorhizobium sp. HRRK 190, Streptomyces sp. HRTK 192, and Novosphingobium sp. HRRK 193) using a series of biochemical tests. Among all strains screened, HRRK 170 had the highest potential for plant growth promotion, given its ability to produce plant growth substances and enzymes such as siderophores and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, respectively, concomitantly with active growth in a wider range of temperatures (10–30 °C) and pH (5.0–10.0). HRRK 170 colonized either as spots or widely on the root surface of all vegetable seedlings tested, but significant growth promotion occurred only in two vegetables (Chinese cabbage and green pepper) within a certain cell density range localized in the plant roots. The results indicate that HRRK 170 could function as a plant growth promoter, but has an optimum cell density for efficient use.

scholarly journals Efficacy of four selective Trichoderma isolates as plant growth promoters in two peanut varieties

2016 ◽  
Vol 4 (2) ◽  
pp. 152
Author(s):  
M. Kamaruzzaman ◽  
M. M. Rahman ◽  
M. S. Islam ◽  
M. U. Ahmad
Keyword(s):  
Plant Growth ◽  
Pathogenic Fungi ◽  
Control Treatment ◽  
Growth Promoter ◽  
Growth Promoters ◽  
Higher Plant ◽  
Potential Growth ◽  
Trichoderma Species ◽  
Wide Range ◽  
Trichoderma Isolates

Trichoderma species are generally used as potential bio control agents against wide range of plant pathogenic fungi and some strains are reported to produce metabolites that enhance plant growth. In the current study we evaluated the four Trichoderma isolates viz. T. harzianum (ST5), T. viride (ST6), T. virens (ST7) and T. atroviride (ST9) including a control were tested as seed treatment against to find out a potential growth-promoter of Peanut. T. harzianum (ST5) gave maximum length and weight of shoot, weight of roots with pods, weight of pods and number of nodules per plant. T. viride (ST6) showed higher plant growth, nodulation and yield compared to T. virens (ST7) and T. atroviride (ST9). Minimum growth, yield and nodulation were observed with control treatment.

scholarly journals Growth promoters in banana seedlings submitted to saline irrigation

2021 ◽  
Vol 29 ◽  
pp. 315-324
Author(s):  
Daniela Andreska da Silva ◽  
Ricardo Leoni Gonçalves Bastos ◽  
Christiana de Fátima Bruce da Silva ◽  
Alan Bernard Oliveira de Sousa
Keyword(s):  
Plant Growth ◽  
Leaf Area ◽  
Plant Height ◽  
Irrigation Water ◽  
Root Length ◽  
Growth Promotion ◽  
Growth Promoter ◽  
Saline Irrigation ◽  
Bacillus Spp ◽  
Number Of Leaves

The objective of this study was to evaluate the effect of inoculation of a plant growth promoter bacteria on the growth of micropropagated banana seedlings cultivar Williams under irrigation with water at different saline levels. The experiment was carried out in a greenhouse at Embrapa Agroindústria Tropical, Fortaleza, State of Ceará. The experimental design was in randomized blocks, in a 3 x 4 factorial scheme, corresponding to the three factors for growth promotion (negative control: water; Osmocote® slow-release fertilizer and a Bacillus spp. bacterium) subjected to four levels of irrigation water salinity (S1 = 0.5; S2 = 1.5; S3 = 3.0 and S4 = 4.5 dS m-1), and five blocks, totaling 60 experimental units. Sixty days after transplanting (DAT) and application of treatments, the following variables related to plant growth were measured: number of leaves (NL), pseudostem diameter (PD), plant height (PH), leaf area (LA), and root length (RL). The rise in saline levels in the irrigation water negatively influenced the variables number of leaves, pseudostem diameter, root length, and leaf area, showing a decreasing linear behavior. The variables number of leaves and leaf area of seedlings inoculated in association with Bacillus spp. did not differ from each other, regardless of the saline level. This indicated a likely increase in the response to the salinity tolerance of the seedlings. Treatment with Osmocote® fertilizer differed statistically for variables plant height, pseudostem diameter, and leaf area.

scholarly journals Draft Genome Sequence of Bacillus altitudinis Lc5, a Biocontrol and Plant Growth-Promoting Endophyte Strain Isolated from Indigenous Black Rice of Manipur

2018 ◽  
Vol 6 (26) ◽  
Author(s):  
Momota Potshangbam ◽  
Dinabandhu Sahoo ◽  
Preveen Verma ◽  
Sandhya Verma ◽  
Mohan Chandra Kalita ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Draft Genome ◽  
Growth Promoter ◽  
Black Rice ◽  
Content Type ◽  
Bacillus Altitudinis ◽  
Genome Study ◽  
Plant Growth Promoting ◽  
Growth Promoting

We report here the 3.6-Mb draft genome of Bacillus altitudinis Lc5, a potential plant growth promoter and an active antagonistic endophyte of black rice. This genome study will provide better insights into the strain’s mechanisms for plant growth promotion and biocontrol, thus facilitating its application in organic agriculture.

scholarly journals Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhen Wang ◽  
Manoj Kumar Solanki ◽  
Zhuo-Xin Yu ◽  
Muhammad Anas ◽  
Deng-Feng Dong ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Defense Enzyme ◽  
Plant Growth Promoting ◽  
Related Enzyme ◽  
Biocontrol Potential

To understand the beneficial interaction of sugarcane rhizosphere actinobacteria in promoting plant growth and managing plant diseases, this study investigated the potential role of sugarcane rhizospheric actinobacteria in promoting plant growth and antagonizing plant pathogens. We isolated 58 actinobacteria from the sugarcane rhizosphere, conducted plant growth-promoting (PGP) characteristics research, and tested the pathogenic fungi in vitro. Results showed that BTU6 (Streptomyces griseorubiginosus), the most representative strain, regulates plant defense enzyme activity and significantly enhances sugarcane smut resistance by regulating stress resistance-related enzyme (substances (POD, PAL, PPO, TP) in sugarcane) activity in sugarcane. The genomic evaluation indicated that BTU6 has the ability to biosynthesize chitinase, β-1,3-glucanase, and various secondary metabolites and plays an essential role in the growth of sugarcane plants under biotic stress. Potential mechanisms of the strain in improving the disease resistance of sugarcane plants and its potential in biodegrading exogenous chemicals were also revealed. This study showed the importance of sugarcane rhizosphere actinobacteria in microbial ecology and plant growth promotion.

scholarly journals Mycogenic Nano-Complex for Plant Growth Promotion and Bio-Control of Pythium aphanidermatum

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1858
Author(s):  
Shaima M.N. Moustafa ◽  
Rania H. Taha
Keyword(s):  
Plant Growth ◽  
Metal Complex ◽  
Vicia Faba ◽  
Growth Promotion ◽  
Pathogenic Fungus ◽  
Pythium Aphanidermatum ◽  
Root Weight ◽  
Total Biomass ◽  
Growth Promoter ◽  
Green Method

(1) Background: biological way is one of the most ecofriendly and safe strategies for nanomaterials synthesis. So, biosynthesis-green method was used for the preparation of Zn(II) complex (in the Nano scale) from the reaction of the schiff base ligand 2,2′-((1E,1′E)-(1,2-phenylenebis (azanylylidene)), bis(methanylylidene))bis(4-bromophenol), and Zn(II)sulphate. The biogenic ZnNP-T was characterized by different methods. Our purpose was to evaluate the ability of biosynthesis-green method for the preparation of Zn(II) complex as an antifungal agent against diseases from fungal species. (2) Methods: in this work, isolates of Pythium aphanidermatum and Trichderma harzianum were obtained, and Trichderma harzianum was used to prepare nano metal complex. We tested the pathogenicity of nano metal complex against seedling and germination of seeds, and we evaluated the effectiveness of ZnNP-T for growth promotion of Vicia feba in early stage and inhibitory activity against Pythium aphanidermatum. (3) Results: antagonistic activity of ZnNP-T was tested in vitro against Pythium aphanidermatum, and then the growth rates of Vicia faba were determined. The obtained data revealed that mycelial growth of pathogenic fungus was inhibited about 73.8% at 20 ppm. In addition, improved the total biomass of Vicia faba in the presence of P. aphanidermatum. All concentration of ZnNP-T positively affected root weight of Vicia faba seedlings, and positively affected shoot weight. Root and shoot lengths were affected by using 20 ppm of ZnNP-T with up to 180 and 96.5 mm of shoot and root length compared to that of the control, while germination percentage was significantly enhanced with up to 100% increase after 72 h of germination. (4) Conclusion: one of the modern challenges in vegetable or fruit production is to enhance seed germination and to grow healthy plants with strong root system. In future, there should be a focus on using of biogenic Zinc nano-complex as plant growth promoter agents.

scholarly journals What do patents tell us about microalgae in agriculture?

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mayara Mari Murata ◽  
Luiz Rodrigo Ito Morioka ◽  
Josemeyre Bonifacio Da Silva Marques ◽  
Alessandra Bosso ◽  
Hélio Hiroshi Suguimoto
Keyword(s):  
Plant Growth ◽  
Agricultural Sector ◽  
Growth Promotion ◽  
The United States ◽  
World Intellectual Property Organization ◽  
European Patent ◽  
Growth Promoter ◽  
Industrial Property ◽  
Alternative Source ◽  
Patent Documents

AbstractMicroalgae have been used widely as a biological source for several industries, such as biofuel, pharmaceutical and food. Recently, the agricultural industry has also began using microalgae as an alternative source for sustainable products to replace agrochemicals. Due to the lack of scientific articles in this research area, the objective of this study was to search for applications of microalgae and to characterize its use in agriculture using the patent documents available in three patent databases, World Intellectual Property Organization (WIPO), European Patent Office (EPO) and Brazilian Institute of Industrial Property (INPI). The search was carried out using the keyword “microalgae” and applying the filter for International Patent Classification (IPC) code “A01N” which corresponds to patents related to agriculture and cultivation of microalgae. Our patent database search returned 669 documents and 132 patents were selected for the study based on their abstracts. The first patent was registered in 1982 and described the use of microalgae Chlorella extract as a plant growth promoter. After that, no patent was registered for 15 years. From 2005 to 2014, only seven patents were found. However, the scenario changed from 2015 when the number of patents increased mainly in the United States, China and Europe. The patent analysis showed several applications for microalgae in the agricultural sector, such as plant growth promotion, biofertilization, plant disease control, weed management, and post-harvest quality. This review confirmed the increasing interest in microalgae-derived products in agriculture and the value of using patent documents to assess innovative areas.

scholarly journals Exploring the Insect Control and Plant Growth Promotion Potentials of Endophytes Isolated From Calotropis procera Present in Jeddah KSA

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091286
Author(s):  
Ihsan Ullah ◽  
Khalid M. S. Al-Ghamdi ◽  
Yasir Anwar ◽  
Jazem A. Mahyoub
Keyword(s):  
Plant Growth ◽  
Inhibitory Concentration ◽  
Galleria Mellonella ◽  
Growth Promotion ◽  
Lateral Roots ◽  
Proteinase K ◽  
Gas Chromatography Mass Spectrometry ◽  
Calotropis Procera ◽  
Growth Promoter ◽  
Plant Growth Hormones

Pseudomonas bacteria are entomopathogenic that can naturally infect and kill insects upon ingestion. The insecticidal and plant growth-promoting roles of the bacteria were assessed by applying Pseudomonas IUK001 to insects and plants. The culture extract (CE) of IUK001 at the concentration of 1 mL/cm3 of an artificial diet was used as a treatment for Galleria mellonella larvae. The CE was heat stable at 70°C for 30 minutes and proteinase-K stable, showing 100% and 90% insecticidal activity against G. mellonella larvae. Gas chromatography–mass spectrometry (GC–MS) analysis revealed the insecticidal compounds, for example, trans-cinnamic acid, ornithine, and cyclo (l-Pro-l-Val) in the CE of IUK001. Antibacterial activity was assessed through minimal inhibitory concentration and half-maximal inhibitory concentration values, which ranged from 28 ± 0.8 and 18.9 ± 0.7 to 8 ± 1.8 and 4.6 ± 0.6, respectively. Moreover, plants were inoculated with IUK001, which significantly enhanced ( P < 0.05) the total plant lengths (root + shoot), dry and fresh biomasses and chlorophyll content, and also induced lateral roots. Plant growth hormones, auxins: indole-3-butyric acid and indole-3- propionic acid, were then analyzed through GC–MS in the CE using their respective isotopic internal standards. The findings of the study suggest that IUK001 is a significantly valuable candidate to be exploited as a biocontrol agent as well as a plant growth promoter.

scholarly journals Rhizodegradation of Pyrene by a Non-pathogenic Klebsiella pneumoniae Isolate Applied With Tagetes erecta L. and Changes in the Rhizobacterial Community

2021 ◽  
Vol 12 ◽  
Author(s):  
Jina Rajkumari ◽  
Yashmin Choudhury ◽  
Kasturi Bhattacharjee ◽  
Piyush Pandey
Keyword(s):  
Klebsiella Pneumoniae ◽  
Plant Growth ◽  
Growth Promotion ◽  
Diversity Index ◽  
Ornamental Plant ◽  
Tagetes Erecta ◽  
Root Weight ◽  
Available Nitrogen ◽  
Core Microbiome ◽  
Bacterial Phyla

The non-clinical Klebsiella pneumoniae variants, isolated from different environments, are now well acknowledged for their role in plant-growth promotion and biodegradation of pollutants. In the present study, a non-clinical environmental isolate K. pneumoniae AWD5 is being described for rhizoremediation of pyrene, applied through the rhizosphere of an ornamental plant, Tagetes erecta L (marigold). The non-pathogenic nature of AWD5 was established using an in vivo mouse model experiment, where AWD5 was unable to cause lung infection in tested mice. Degradation of pyrene, in the presence of succinate as co-substrate, was observed to be 87.5% by AWD5, after 21 days of incubation in minimal (Bushnell–Hass) medium in vitro conditions. Consequently, the bacterial inoculation through the rhizosphere of T. erecta L. plants resulted in 68.61% degradation of pyrene, which was significantly higher than control soil. Inoculation of AWD5 also improved plant growth and exhibited an increase in root length (14.64%), dry root weight (80.56%), shoot length (3.26%), and dry shoot weight (45.35%) after 60 days of incubation. T. erecta L., an ornamental plant, was also found to be suitable for bioremediation of pyrene. The effect of AWD5 application, and rhizoremediation process, on rhizosphere bacterial diversity and community structure has been studied using the metagenomic analysis of the 16S (V3–V4) region of rRNA. 37 bacterial phyla constituted the core microbiome, which was dominated by Proteobacteria followed by Actinobacteria, Actinobacteria, and Planctomycetes for all the treatments. AWD5 inoculation enhanced the relative abundance of Firmicutes and Acidobacteria as compared with other treatments. Genus Kaistobacter and Verrucomicrobia were found to be an abundant indigenous population in pyrene-spiked soils. Bacterial richness and diversity were analyzed using the Shannon–Wiener (H) index. A lower diversity index was observed in pyrene-spiked soils. Canonical correspondence analysis (CCA) showed a possible linkage with plant growth attributes and available nitrogen content that influences diversity and abundance of the bacterial community.

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