The Mangrove-Derived Diterpenoid  Diaporthe B Inhibits the Stemness  and Increases the Efficacy of Docetaxel  in Prostate Cancer PC-3 Cells

The absolute configuration of diaporthe B, a pimarane diterpene isolated from the mangrove derived endophytic fungus Eutypella sp #3E, was determined by a single-crystal x-ray diffraction study. The present study aimed to investigate the effects of diaporthe B on docetaxel-resistant prostate cancer PC-3 cells. Results of our studies showed that docetaxel-resistant PC-3 cells had higher sphere-forming efficiency and an increase in adherence to collagen-coated culture plates. The protein levels of cancer stem cell (CSC)-related markers CD44, CD133, and ALDH1A1 were higher in the docetaxel-resistant PC-3 cells than in the parental cells. Treatment with diaporthe B dose-dependently inhibited the growth and induced apoptosis in the resistant cells. Moreover, diaporthe B treatment decreased the sphere-forming efficiency and the adherence to collagen-coated plates in docetaxel-resistant PC-3 cells. Diaporthe B also decreased the protein levels of CSC-related markers CD44, CD133, and ALDH1A1 in the resistant cells. In addition, a combination of diaporthe B and docetaxel had a more potent effect on growth inhibition and apoptosis in the resistant cells than either agent alone. Our studies suggest that diaporthe B inhibits the stemness of prostate cancer cells and may have therapeutic potential for enhancing the efficacy of docetaxel in docetaxel-resistant prostate cancer cells.

Download Full-text

Cyclooxygenase-2 inhibitor celecoxib augments chemotherapeutic drug-induced apoptosis by enhancing activation of caspase-3 and -9 in prostate cancer cells

International Journal of Cancer ◽

10.1002/ijc.20878 ◽

2005 ◽

Vol 115 (3) ◽

pp. 484-492 ◽

Cited By ~ 71

Author(s):

Devendra S. Dandekar ◽

Monica Lopez ◽

Robert I. Carey ◽

Bal L. Lokeshwar

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Caspase 3 ◽

Cyclooxygenase 2 ◽

Chemotherapeutic Drug ◽

Prostate Cancer Cells ◽

Drug Induced ◽

Induced Apoptosis

Download Full-text

Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

International Journal of Oncology ◽

10.3892/ijo.2012.1527 ◽

2012 ◽

Vol 41 (3) ◽

pp. 818-828 ◽

Cited By ~ 42

Author(s):

EWELINA SZLISZKA ◽

GRZEGORZ ZYDOWICZ ◽

ELZBIETA MIZGALA ◽

WOJCIECH KROL

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Hydroxycinnamic Acid ◽

Prostate Cancer Cells ◽

Artepillin C ◽

Induced Apoptosis

Download Full-text

MP24-05 TARGETING THE VAV3 ONCOGENE ENHANCES DOCETAXEL-INDUCED APOPTOSIS THROUGH THE INHIBITION OF ANDROGEN RECEPTOR PHOSPHORYLATION IN LNCAP PROSTATE CANCER CELLS UNDER CHRONIC HYPOXIA IN VITRO AND IN VIVO

The Journal of Urology ◽

10.1016/j.juro.2014.02.286 ◽

2014 ◽

Vol 191 (4S) ◽

Author(s):

Takeo Nomura ◽

Mutsushi Yamasaki ◽

Kenichi Hirai ◽

Toru Inoue ◽

Ryuta Sato ◽

...

Keyword(s):

Prostate Cancer ◽

Androgen Receptor ◽

Cancer Cells ◽

Chronic Hypoxia ◽

Prostate Cancer Cells ◽

Receptor Phosphorylation ◽

Induced Apoptosis

Download Full-text

Plumbagin-induced Apoptosis in Human Prostate Cancer Cells is Associated with Modulation of Cellular Redox Status and Generation of Reactive Oxygen Species

Pharmaceutical Research ◽

10.1007/s11095-008-9533-3 ◽

2008 ◽

Vol 25 (9) ◽

pp. 2171-2180 ◽

Cited By ~ 77

Author(s):

Anna A. Powolny ◽

Shivendra V. Singh

Keyword(s):

Prostate Cancer ◽

Reactive Oxygen Species ◽

Cancer Cells ◽

Redox Status ◽

Human Prostate ◽

Oxygen Species ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Cellular Redox ◽

Induced Apoptosis

Download Full-text

Newly synthesized benzimidazoles inhibits vascular endothelial growth factor and matrix metalloproteinase-2 and -9 levels in prostate cancer cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520621666210924114856 ◽

2021 ◽

Vol 21 ◽

Author(s):

Suleyman Ilhan ◽

Gamze Dilekci ◽

Adem Guner ◽

Hakan Bektas

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Molecular Mechanisms ◽

Umbilical Vein ◽

Imidazole Ring ◽

Matrix Metalloproteinase 2 ◽

Benzimidazole Derivatives ◽

Prostate Cancer Cells ◽

Protein Levels ◽

Umbilical Vein Endothelial Cells

Background: Investigating the effects of newly synthesized agents on various molecular mechanisms to understand their mechanism of action is an important step of pre-clinical screening. Benzimidazoles are composed of a unique fused benzene and imidazole ring and have attracted great attention due to their broad bioactivities, including antitumor. Objective: In the current study, we reported the synthesis of novel benzimidazole derivatives and investigated the possible cytotoxic and anti-angiogenic effects on human prostate cancer and umbilical vein endothelial cells (HUVECs). Methods: MTT assay was used to assess cell viability. A scratch assay was conducted to monitor the migration of cells. mRNA expression levels of VEGF, MMP-2, and MMP-9 were evaluated using qPCR. Changes in protein levels were evaluated by western blotting. Results: Compound G1, having a chlorine moiety, showed a potent cytotoxic activity on both prostate cancer cells and HUVECs, and inhibited cell migration via decreasing the mRNA and protein levels of key angiogenesis-related molecules such as VEGF, MMP-2, and MMP-9. Conclusion: These results suggest that newly synthesized G1 may be a novel anti-angiogenic agent for prostate cancer treatment.

Download Full-text