Increasing Concentration of Sinovial: Effect on Cartilage Protection in a Rabbit ACLT Model

Cartilage ◽  
2020 ◽  
pp. 194760352090536
Author(s):  
Pomme Boissier ◽  
Pierre Mainil-Varlet ◽  
Giuseppe R. Mautone
Keyword(s):  
Cruciate Ligament ◽  
Intraoperative Complications ◽  
Cartilage Degradation ◽  
Research Society ◽  
Control Group ◽  
Dependent Effect ◽  
India Ink ◽  
Anterior Cruciate ◽  
Dose Dependent ◽  
Dose Dependent Effect

Objective This study aimed to test the hypothesis that administration of increasing doses of Sinovial (hyaluronic acid [HA]), would exhibit a dose-dependent effect on the prevention of cartilage degradation, without local and systemic toxicity. Methods Twenty-seven adult rabbits were subjected to anterior cruciate ligament transection (ACLT). Two Sinovial products containing HA concentrations of 1.6% and 2.4% were used as active treatment, and 0.9% saline was used as control and injected intra-articularly 7 days post ACLT. Radiographs were taken prior to surgery, at injection and sacrifice times. After euthanasia, 8 weeks postsurgery, knee joints were observed macroscopically using India ink staining with OARSI (Osteoarthritis Research Society International) scoring and histologically using modified Mankin scoring. The synovial membranes were analyzed using Cake classification. Results No intraoperative complications were observed. One week postinjection, 4 animals in the HA 2.4% group developed subcutaneous nodules that disappeared spontaneously. No inflammation of the synovial membrane was ever observed. The control group exhibited the maximum uptake of India ink 2.22 ± 0.14. HA groups exhibited a dose-dependent ( P = 0.02) reduction in India ink uptake: 1.75 ± 0.17 for HA 1.6% and 1.58 ± 0.14 for HA 2.4%. The most marked dose-dependent effect of this study was a reduction of modified Mankin score for HA groups, with the 2.4% treatment achieving a statistically significant improvement as compared with the control group (7.19 ± 0.85 for saline, 4.65 ± 0.66 for HA 1.6%, and 3.53 ± 0.59 for HA 2.4%; P = 0.005). Conclusions A dose-dependent protective effect on cartilage was observed after injection of both HA solutions.

117 EFFECT OF RESVERATROL ON THE CULTURE OF PORCINE EMBRYOS

2009 ◽  
Vol 21 (1) ◽  
pp. 158
Author(s):  
K. Lee ◽  
C. Wang ◽  
D. Miller ◽  
Z. Machaty
Keyword(s):  
Caspase 3 ◽  
Total Cell ◽  
Control Group ◽  
Blastocyst Formation ◽  
Dependent Effect ◽  
Expression Levels ◽  
Cell Numbers ◽  
Apoptotic Activity ◽  
Dose Dependent ◽  
Dose Dependent Effect

Resveratrol (3,4′,5-trihydroxystilbene) is a phytoalexin present in a variety of dietary sources including grapes, plums and peanuts. It was shown to exert a wide variety of pharmacological activities ranging from anti-inflammatory effects to immunomodulation and chemoprevention. The aim of this study was to investigate the effect of resveratrol on porcine embryo development. In vitro-matured pig oocytes were activated by two direct current electric pulses (60 μ seconds each) followed by an incubation with 10 μg mL–1 cycloheximide and 7.5 μg mL–1 cytochalasin B, for 5 h. Activated oocytes (in groups of 10) were then placed in 20 μL drops of Porcine Zygote Medium 3 (PZM-3) supplemented with various concentrations of resveratrol (0 μm, 0.5 μm, 25 μm) and cultured for 7 days. The frequency of blastocyst formation in each group was recorded and compared by Chi-square test; the total cell numbers of the blastocysts from the two best groups (control and 0.5 μm) were counted and compared by Student’s t-test. In addition, expression levels of three genes related to apoptosis (Bax, Bcl-2, and Caspase-3) were quantified in the blastocysts by quantitative real-time RT-PCR. The analysis was repeated three times, and differences in gene expression were compared by ANOVA. Differences at P < 0.05 were considered significant. Incubation of parthenotes with 25 μm resveratrol decreased blastocyst formation from 28.1% (n = 89; control group) to 4.5% (n = 66; P < 0.05). However, when parthenogenetic embryos were cultured in the presence of 0.5 μm resveratrol, blastocyst formation increased significantly: whereas only 33.0% of control embryos (n = 312) reached the blastocyst stage, this percentage in the resveratrol-treated group was 43.5% (n = 301; P < 0.05). The average total cell numbers in control and 0.5 μm resveratrol-treated blastocysts were 42.4 ± 2.1 and 44.0 ± 1.9, respectively; the difference was not statistically significant. Finally, lower expression of the Bcl-2 and Caspase-3 genes was observed in the embryos cultured with 0.5 μm resveratrol. Evidences indicated that resveratrol has a dose-dependent effect on cells. At high concentrations resveratrol exerted an antiproliferation effect, whereas at low concentration it promoted cell division. In this study a similar dose-dependent effect of resveratrol was found. The presence of 25 μm resveratrol had a negative effect on embryonic development. However, 0.5 μm resveratrol in the culture medium induced higher blastocyst formation compared with the control group. The presence of resveratrol also affected expression levels of genes related to apoptosis. Decreased levels of Bcl-2 suggest that resveratrol may suppress the mitochondria-related anti-apoptotic activity; whereas down-regulated Caspase-3 indicates a decreased apoptotic activity in the embryos treated with resveratrol. Our results suggest that the treatment of pig embryos with 0.5 μm of resveratrol has a beneficial effect on preimplantation development leading to improved blastocyst formation. The impact of resveratrol on apoptosis needs further investigation.

Dose-dependent effect of testosterone on the induction of eclipse coloration in castrated wild mallard drakes (Anas platyrhynchos L.)

1992 ◽  
Vol 70 (3) ◽  
pp. 428-431 ◽  
Author(s):  
Eberhard Haase ◽  
Reiner Schmedemann
Keyword(s):  
Breeding Season ◽  
Control Group ◽  
Dependent Effect ◽  
Daily Dose ◽  
Natural Breeding ◽  
Dose Dependent ◽  
Dose Dependent Effect ◽  
Wild Mallard ◽  
Intermediate Pattern

Four groups of adult wild mallard drakes that had been previously castrated were injected daily with 5, 1, or 0.2 mg testosterone dissolved in propanediol or with the vehicle alone. The injections started on February 29 and continued for 64 days during the natural breeding season of the species. The birds were kept in outdoor aviaries in a seminatural environment in Kiel (54°N). By the end of the postnuptial molt in mid-July the groups differed markedly in coloration. The vehicle-injected control group had regained an almost typical nuptial plumage, whereas the castrates that had received the highest daily dose of testosterone exhibited an almost complete eclipse plumage. The two other groups showed an intermediate pattern, with about 40% eclipse coloration in the group injected with 1 mg testosterone/day and about 10% eclipse coloration in the group that had received 0.2 mg testosterone/day. The possible role of the aromatization of testosterone to estrogens in the induction of the eclipse coloration is discussed.

Altered Knee Laxity and Stiffness in Response to a Soccer Match Simulation in Players Returning to Sport Within 12 Months After Anterior Cruciate Ligament Reconstruction

2021 ◽  
pp. 036354652110130
Author(s):  
Stefano Nuccio ◽  
Luciana Labanca ◽  
Jacopo Emanuele Rocchi ◽  
Pier Paolo Mariani ◽  
Paola Sbriccoli ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Return To Sport ◽  
Knee Laxity ◽  
Activity Level ◽  
Soccer Players ◽  
Control Group ◽  
Soccer Match ◽  
Anterior Cruciate ◽  
Anterior Knee

Background: The acute effects of exercise on anterior knee laxity (AKL) and anterior knee stiffness (AKS) have been documented in healthy participants, but only limited evidence has been provided for athletes cleared to return to sports after anterior cruciate ligament (ACL) reconstruction (ACLR). Purpose/Hypothesis: The purpose was to determine if 45 minutes of a soccer match simulation lead to acute changes in AKL and AKS in soccer players returning to sport within 12 months after ACLR. We hypothesized that the reconstructed knee of the ACLR group would exhibit an altered response to sport-specific exercise. Study Design: Controlled laboratory study. Methods: A total of 13 soccer players cleared to return to sport after ACLR and 13 healthy control soccer players matched for age, physical activity level, limb dominance, and anthropometric characteristics were recruited. To assess the effects of a standardized soccer match simulation (Soccer Aerobic Field Test [SAFT45]) on AKL and AKS, an arthrometric evaluation was carried out bilaterally before and immediately after SAFT45. To conduct a comprehensive examination of the force-displacement curve, the absolute and side-to-side difference (SSD) values of both AKL and AKS were extracted at 67, 134, and 200 N. Results: The ACLR and control groups showed similar AKL and AKS at baseline ( P > .05). In response to SAFT45, laxity increased bilaterally at all force levels by 14% to 17% only in the control group ( P < .025). Similarly, AKS at 134 and 200 N decreased in response to SAFT45 only in the control group (10.5% and 20.5%, respectively; P < .025). After SAFT45, the ACLR group had 1.9 and 2.5 times higher SSDs of AKS at 67 and 134 N compared with the control group, respectively ( P < .025), as well as a 1.9 times higher SSD of AKS at 134 N compared with baseline ( P = .014). Conclusion: Soccer players at the time of return to sport after ACLR showed an altered mechanical response to a sport-specific match simulation consisting of bilaterally unchanged AKL and AKS. Clinical Relevance: Soccer players showing altered AKL and AKS in response to exercise after ACLR may not be ready to sustain their preinjury levels of sport, thus potentially increasing the risk of second ACL injuries.

scholarly journals Silver‐doped sol‐gel borate glasses: Dose‐dependent effect on Pseudomonas aeruginosa biofilms and keratinocyte function

2021 ◽  
Author(s):  
Shiva Naseri ◽  
Gabriele Griffanti ◽  
William C. Lepry ◽  
Vimal B. Maisuria ◽  
Nathalie Tufenkji ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sol Gel ◽  
Borate Glasses ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect

scholarly journals Treadmill Exercise after Controlled Abnormal Joint Movement Inhibits Cartilage Degeneration and Synovitis

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 303
Author(s):  
Yuichiro Oka ◽  
Kenij Murata ◽  
Kaichi Ozone ◽  
Takuma Kano ◽  
Yuki Minegishi ◽  
...  
Keyword(s):  
Treadmill Exercise ◽  
Cruciate Ligament ◽  
Cartilage Degeneration ◽  
Research Society ◽  
Therapeutic Effects ◽  
Joint Movement ◽  
Knee Oa ◽  
Anterior Cruciate ◽  
Safranin O ◽  
Walking Group

Cartilage degeneration is the main pathological component of knee osteoarthritis (OA), but no effective treatment for its control exists. Although exercise can inhibit OA, the abnormal joint movement with knee OA must be managed to perform exercise. Our aims were to determine how controlling abnormal joint movement and treadmill exercise can suppress cartilage degeneration, to analyze the tissues surrounding articular cartilage, and to clarify the effect of treatment. Twelve-week-old ICR mice (n = 24) underwent anterior cruciate ligament transection (ACL-T) surgery on their right knees and were divided into three groups as follows: ACL-T, animals in the walking group subjected to ACL-T; controlled abnormal joint movement (CAJM), and CAJM with exercise (CAJM + Ex) (n = 8/group). Walking-group animals were subjected to treadmill exercise 6 weeks after surgery, including walking for 18 m/min, 30 min/day, 3 days/week for 8 weeks. Safranin-O staining, hematoxylin-eosin staining, and immunohistochemical staining were performed. The OARSI (Osteoarthritis research Society international) score was lower in the CAJM group than in the ACL-T group and was even lower in the CAJM + Ex group. The CAJM group had a lower meniscal injury score than the ACL-T group, and the CAJM + Ex group demonstrated a less severe synovitis than the ACL-T and CAJM groups. The observed difference in the perichondrium tissue damage score depending on the intervention method suggests different therapeutic effects, that normalizing joint motion can solve local problems in the knee joint, and that the anti-inflammatory effect of treadmill exercise can suppress cartilage degeneration.

How to Achieve an Accurate Anatomical Femoral Tunnel Technique in ACL Reconstruction in the Early Years of Your Consultancy? Femoral Offset Aimer Technique: Consistent and Reproducible Technique

2019 ◽  
Vol 33 (12) ◽  
pp. 1201-1205
Author(s):  
Pregash Ellapparadja ◽  
Ignatius Joseph ◽  
Veenesh Selvaratnam
Keyword(s):  
Femoral Tunnel ◽  
Cruciate Ligament ◽  
Intraoperative Complications ◽  
Senior Author ◽  
Early Years ◽  
Anatomical Location ◽  
Independent Practice ◽  
Femoral Offset ◽  
Tunnel Technique ◽  
Anterior Cruciate

AbstractFemoral tunnel malposition is the most common reason for failure of primary anterior cruciate ligament reconstruction. There are several methods to identify the anatomical location of femoral footprint. Femoral offset aimer technique is one such technique which is easy to use and reliable. It is an ideal technique for junior and inexperienced surgeons to recreate the femoral tunnel in its anatomical footprint. The senior author (P.E.) has been using this technique for 30 consecutive cases in his first year of independent practice during his consultancy without any major intraoperative complications. The author describes this technique in this article with tips and tricks which will especially guide the junior and inexperienced surgeons to avoid running into intraoperative problems while drilling the femoral tunnel.

Dose-Dependent Effect of Statins on the Incidence of Acute Kidney Injury After Cardiac Surgery

2011 ◽  
Vol 91 (2) ◽  
pp. 520-525 ◽  
Author(s):  
Salima Mithani ◽  
Michael Kuskowski ◽  
Yelena Slinin ◽  
Areef Ishani ◽  
Edward McFalls ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Cardiac Surgery ◽  
Kidney Injury ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect
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