Drug-related talaromycosis: A case report

Talaromycosis is a rare deep fungal infection caused by Talaromyces marneffei. Currently, methamphetamine has become the second-largest drug abuse category in the world after cannabis and has become a serious public health problem. Methamphetamine can inhibit human immune system and increase the probability of pathogenic microorganism infection. On 8 October 2016, a 20-year-old man with a fever history of 2 months was admitted to our hospital. He had bloody stools and abdominal pain during hospitalization. There was no significant abnormality in physical examination. Because of the misdiagnosis, he underwent improper treatment. Periodic acid-Schiff stain (PAS) staining showed that the mucosa of distal ileum, ascending colon, transverse colon, and sigmoid colon were infiltrated by a large number of tissue cells, which contained a large number of blue purple particles. In addition, a large number of histiocytes and multinucleated giant cells can be seen in the lamina propria of ileum mucosa, and fungal spores can be seen in histiocytes. Finally, he was diagnosed as talaromycosis and took itraconazole 0.2 g twice a day. After 5 days, the temperature dropped to normal and the inflammation disappeared, and he continued to take itraconazole for 6 months. Due to the neglect of the history of drug abuse and the concealment, drug-related talaromycosis is often misdiagnosed. Pathological examination is warranted for diagnosis talaromycosis. This condition requires a long-term anti-fungal therapy.

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Candida Chorioamnionitis in Mothers with Gestational Diabetes Mellitus: A Report of Two Cases

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18147450 ◽

2021 ◽

Vol 18 (14) ◽

pp. 7450

Author(s):

Elia Shazniza Shaaya ◽

Siti Atiqah Abdul Halim ◽

Ka Wen Leong ◽

Kevin Boon Ping Ku ◽

Pei Shan Lim ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Umbilical Cord ◽

Neonatal Death ◽

Periodic Acid ◽

Careful Examination ◽

Periodic Acid Schiff ◽

History Of ◽

Methenamine Silver

Background:Candida chorioamnionitis is rarely encountered, even though vulvovaginal candidiasis incidence is about 15%. Interestingly, it has characteristic gross and histological findings on the umbilical cord that are not to be missed. Case Report: We report two cases of Candida chorioamnionitis with presence of multiple yellowish and red spots of the surface of the umbilical cord. Microscopically, these consist of microabscesses with evidence of fungal yeasts and pseudohyphae. The yeasts and pseudohyphae were highlighted by periodic acid– Schiff and Grocott methenamine silver histochemical stains. Both cases were associated with a history of gestational diabetes mellitus. Discussion: Peripheral funisitis is a characteristic feature of Candida chorioamnionitis. It is associated with high risk of adverse perinatal and neonatal outcomes, such as preterm delivery, stillbirth and neonatal death. We recommend careful examination of the umbilical cord of mothers with gestational diabetes mellitus.

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Dematiaceous fungal invasion of a bandage contact lens

BMJ Case Reports ◽

10.1136/bcr-2020-240029 ◽

2021 ◽

Vol 14 (2) ◽

pp. e240029

Author(s):

Anirban Dutta ◽

Sujata Das ◽

Himanshu Sekhara Behera ◽

Ruchi Mittal

Keyword(s):

Contact Lens ◽

Periodic Acid ◽

Significant Risk ◽

Dematiaceous Fungi ◽

Periodic Acid Schiff ◽

Bandage Contact Lens ◽

History Of ◽

Eye Examination ◽

The Right

A 61-year-old man presented with a 1-month history of reduced vision, redness and pain in the right eye. Examination revealed a bandage contact lens (BCL) in situ with diffuse, pigmented deposits. On removal, the underlying cornea was found to be clear. He had been prescribed the BCL 6 months ago following a deep-seated corneal foreign body removal and was unable to follow-up subsequently.The BCL was sent for microbiological and histopathological evaluation. The culture revealed growth of Cladosporium spp, a dematiaceous fungi. Periodic acid–Schiff staining revealed infiltration of pigmented fungal filaments into the substance of the BCL.While contact lens deposits are a frequent finding, fungal deposits are seldom noted. Irregular follow-up and improper lens maintenance are significant risk factors for the same. Early identification and subsequent removal of the lens is vital to prevent infection of the underlying ocular structures.

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Primary cutaneous Aspergillus fumigatus infection in immunocompetent host

BMJ Case Reports ◽

10.1136/bcr-2019-233020 ◽

2020 ◽

Vol 13 (2) ◽

pp. e233020

Author(s):

Pradeep Kumar Mada ◽

Daniel Alexander Saldaña Koppel ◽

Majd Al Shaarani ◽

Andrew Stevenson Joel Chandranesan

Keyword(s):

Periodic Acid ◽

Fungal Spores ◽

Penetrating Trauma ◽

Trauma Patients ◽

Immunocompetent Host ◽

Skin Injury ◽

Periodic Acid Schiff ◽

Incision And Drainage ◽

Systemic Spread ◽

Methenamine Silver

Primary cutaneous aspergillosis (PCA) occurs through inoculation of fungal spores directly into the skin from the environment through disrupted skin such as in burns, surgery or penetrating trauma patients. Most cases reported in literature were in the immunocompromised, rarely in immunocompetent patients. The characteristic lesion of cutaneous aspergillosis is a black eschar on a red plaque, or nodule at the site of skin injury. The diagnosis of PCA can be made by identifying hyphal forms on routine H&E staining or special stains such as periodic acid-Schiff or Gomori methenamine-silver stains on skin biopsy and by fungal cultures. We report a case of an 80-year-old farmer who developed cutaneous aspergillosis after a surgical procedure without any systemic spread. The diagnosis was made by histopathology and tissue fungal cultures. He was treated with incision and drainage followed by oral voriconazole for 4 weeks; which led to clinical recovery.

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Scrofuloderma: A Diagnosis to Bear in Mind in the Western World

Acta Médica Portuguesa ◽

10.20344/amp.10329 ◽

2019 ◽

Vol 32 (4) ◽

pp. 313

Author(s):

Mariana Batista ◽

Barbara Ferreira ◽

Gonçalo Cruz ◽

Américo Figueiredo

Keyword(s):

Periodic Acid ◽

Previous History ◽

Complete Response ◽

Purulent Discharge ◽

Western World ◽

Periodic Acid Schiff ◽

Cutaneous Tuberculosis ◽

Immunodeficiency Virus ◽

History Of ◽

Polymerase Chain

The incidence of tuberculosis has been increasing worldwide. Contrarily, a recent decrease in Portugal has been reported. Cutaneous tuberculosis comprises a low percentage of all cases. We report a 70-year-old female with a 2-month-history of painful, nodular, suppurative lesions in the groin area, bilaterally. Previous history was remarkable for Human Immunodeficiency Virus infection and stage-IIIB cervical cancer. A skin biopsy, stained with periodic acid–Schiff and Fite’s stain, polymerase chain reaction on purulent discharge and mycobacterial culture of the skin were performed, leading to the diagnosis of scrofuloderma. Tuberculostatic therapy was initiated and complete response was observed. This case depicts an uncommon variant of tuberculosis, highlighting the need for awareness of the cutaneous variants of tuberculosis that, although rare, can still present in the clinic today.

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Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2016000100002 ◽

2016 ◽

Vol 36 (1) ◽

pp. 13-18 ◽

Cited By ~ 1

Author(s):

Leonardo R. de Lima ◽

Heloisa M.F. Mendes ◽

Frederico M. Soriani ◽

Danielle G. de Souza ◽

Geraldo Eleno S. Alves ◽

...

Keyword(s):

Chemokine Receptors ◽

Periodic Acid ◽

Previous History ◽

Basal Membrane ◽

Treated Group ◽

Periodic Acid Schiff ◽

Chemokine Signaling ◽

Mixed Breed ◽

History Of ◽

Membrane Changes

Abstract: With the hypothesis that blocking chemokine signaling can ameliorate acute laminitis, the aim was to evaluate the therapeutic effect of intravenous DF1681B, a selective antagonist for CXCR1 and CXCR2 (chemokine receptors), in an oligofructose equine laminitis model. To twelve mixed breed clinically healthy hoses with no previous history of hoof-related lameness was administered oligofructose (10g/kg given by nasogastric tube) and divided into two groups: treated (intravenous DF1681B at 30mg/kg 6, 12, 18, and 24h after oligofructose) and non-treated groups. Laminar biopsies were performed before and 12, 36, and 72h after administering oligofructose. Samples were stained with periodic acid-Schiff (PAS) and scored from 0 to 6 according to epidermal cell and basal membrane changes. The IL-1β, IL-6, and CXCL1 RNA expressions were determined by RT-PCR. Parametric and non-parametric tests were used to compare times within each group (P<0.05). The PAS grades and IL-1β and IL-6 RNA expression increased in the non-treated group, but remained constant in the treated horses. In conclusion, DF1681B therapy reduced laminar inflammation and epidermal deterioration in treated horses. CXCR1/2 blockage should be considered therapeutically for equine acute laminitis.

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Crystal-Storing Histiocytosis as a Cause of Symptomatic Cardiac Mass

Archives of Pathology & Laboratory Medicine ◽

10.5858/133.11.1861 ◽

2009 ◽

Vol 133 (11) ◽

pp. 1861-1864 ◽

Cited By ~ 3

Author(s):

Charles J. Sailey ◽

Borislav A. Alexiev ◽

James S. Gammie ◽

Paula Pinell-Salles ◽

J. Lawrence Stafford ◽

...

Keyword(s):

Monoclonal Gammopathy ◽

Plasma Cells ◽

Periodic Acid ◽

Right Atrial ◽

Prior History ◽

Low Grade ◽

Periodic Acid Schiff ◽

Right Atrial Mass ◽

History Of

Abstract Crystal-storing histiocytosis is a rare disorder that is typically associated with low-grade B-cell lymphomas and monoclonal gammopathy. We present a 64-year-old man with a prior history of weakness and weight loss and hematologic evaluation that had revealed immunoglobulin G κ monoclonal light chains in the serum and negative bone marrow biopsy. He presented with supraventricular tachyarrhythmia and a right atrial mass seen on echocardiogram and excised surgically. Histologically, the tumor was composed of sheets of macrophages infiltrating the atrial myocardium. The histiocytes were filled with multiple needle-shaped, periodic acid-Schiff–negative crystals. These cells and associated plasma cells failed to show clonal light chain restriction by in situ hybridization or immunohistochemistry, and there was no area of lymphoma in the tumor. Ultrastructural examination showed numerous sticklike, trapezoidal, or polygonal dense crystals in the cytoplasm of histiocytes corroborating the diagnosis of crystal-storing histiocytosis. Although rare, crystal-storing histiocytosis should be included in the differential diagnosis of heart masses in patients with hematologic conditions associated with monoclonal gammopathy.

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A case of systemic mycosis in a Hovawart dog due to Candida albicans

Veterinární Medicína ◽

10.17221/1561-vetmed ◽

2011 ◽

Vol 56 (No. 5) ◽

pp. 260-264 ◽

Cited By ~ 6

Author(s):

M. Skoric ◽

P. Fictum ◽

I. Slana ◽

P. Kriz ◽

I. Pavlik

Keyword(s):

Candida Albicans ◽

Histopathological Examination ◽

Periodic Acid ◽

Abdominal Cavity ◽

Giant Cells ◽

Health Condition ◽

Periodic Acid Schiff ◽

Tissue Samples ◽

Generalized Lymphadenopathy ◽

Systemic Infections

Candida albicans is reported as the etiological agent of multi-systemic infections in dogs. A two-year-old female Hovawart dog was presented with marked alteration in its health condition characterised by weakness, fever, anorexia, abdominal pain, cachexy and generalized lymphadenopathy. A radiograph of the abdominal cavity showed several non-specific nodular lesions in the mesentery, ranging in size up to 10 cm in diameter. At necropsy, extensive enlargement of lymph nodes and the presence of numerous whitish to grey nodules of different sizes in several organs were evident. Histopathological examination revealed pyogranulomatous inflammation characterized by large areas of necrosis surrounded by neutrophilic granulocytes, macrophages, multinucleated giant cells, and a variable admixture of lymphocytes and fungi-like organismsin in all affected organs. Numerous branching hyphae, subsequently identified by mycological cultivation as Candida albicans, were observed. A periodic acid Schiff (PAS) reaction to prove the presence of fungi in tissues was positive. Examination of tissue samples of affected organs using polymerase chain reaction (quantitative Real-Time PCR) and cultivation was negative for the presence of all members of the Mycobacterium tuberculosis complex, M. avium subsp. avium and M. avium subsp. hominissuis.

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Peritoneal Sarcoidosis Mimicking Peritoneal Tuberculosis and Advanced Ovarian Carcinoma

Case Reports in Obstetrics and Gynecology ◽

10.1155/2020/1905649 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Brittany N. Robles ◽

Catherine Shea ◽

Ghadir Salame

Keyword(s):

Local Community ◽

Periodic Acid ◽

Granulomatous Inflammation ◽

Unknown Origin ◽

Cyst Formation ◽

Giant Cells ◽

African American Female ◽

Peritoneal Tuberculosis ◽

Periodic Acid Schiff ◽

Antituberculosis Treatment

Sarcoidosis is an inflammatory disease that affects one or multiple organs, most commonly the lungs and lymph nodes. This disease can present in a variety of ways which often makes diagnosis difficult. A 54-year-old postmenopausal African American female with a history of omental carcinomatosis of unknown origin was referred to the gynecology-oncology service at a local community hospital following a laparoscopic incarcerated hernia repair where multiple abdominal lesions suspicious of ovarian carcinomatosis were visualized. She was brought to the operating room for a diagnostic laparoscopy at which point the intra-abdominal survey revealed white tubercle-like lesions that were consistent with peritoneal tuberculosis. The lesions were excised and sent to pathology. The omentum biopsy was originally reported as adipose tissue showing focal fibrosis, focal mild acute inflammation, few cyst formation, and multiple granulomatous chronic inflammation, with multinucleated giant cells. Periodic acid-Schiff stain and acid fast bacilli stain were negative, and a diagnosis of peritoneal tuberculosis was made. The patient was started on an antituberculosis treatment regimen; however, she was not improving. The pathology slides were reexamined and revealed nonnecrotizing granulomatous inflammation consistent with sarcoidosis. The patient was immediately referred to the department of pulmonology and rheumatology, at which point she was started on corticosteroids and had an improvement in her condition.

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Actinomycosis of bilateral psoas muscle: a case report

MedPharmRes ◽

10.32895/ump.mpr.5.2.2 ◽

2021 ◽

Vol 5 (2) ◽

pp. 7-11

Author(s):

Hoang Huu Bui ◽

Thao Thi Phuong Doan ◽

Tuan Ngoc Cao ◽

Tuong Trung Dinh ◽

Chuong Dinh Nguyen

Keyword(s):

Intrauterine Device ◽

Periodic Acid ◽

Pelvic Mass ◽

Psoas Muscle ◽

Invasive Disease ◽

Periodic Acid Schiff ◽

Muscle Involvement ◽

History Of ◽

Left Psoas Muscle ◽

The Right

Actinomycosis is an underreported chronic invasive disease caused by Actinomyces spp. In that group of diseases, pelvic actinomycosis is very rare and most of the cases reported in the literature have shown unilateral psoas muscle involvement. We report a 35-year-old woman with a retroperitoneal mass located in the left psoas muscle that makes abdominal tenderness and difficulty in walking. She had a 7-year history of intrauterine device which had been removed one month just before this event. After undergoing two laparotomies to perform a biopsy with undetermined results, she developed a new mass in the right psoas muscle which was similar to the left one. On the third attempt, the histopathology revealed yellow sulfur granules of Actinomyces which reacted positively with periodic acid Schiff and Grocott’s dye. After three months of treatment with Amoxicillin plus Clavulanic acid, her abscesses were completely resolved. Actinomycosis should always be included in the diagnostic list in patients with a pelvic mass and a history of intrauterine device.

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Perineal swelling secondary to a vaginal wall cyst in a bitch in dioestrus

Veterinary Record Case Reports ◽

10.1136/vetreccr-2018-000805 ◽

2019 ◽

Vol 7 (3) ◽

pp. e000805

Author(s):

Cesar Sanchez Jimenez ◽

Ana Rita Ramalho Furtado ◽

Valéria Café Marçal ◽

Darren Barnes ◽

Emilie Fauchon

Keyword(s):

Reproductive Tract ◽

Periodic Acid ◽

Vaginal Wall ◽

Cystic Mass ◽

Female Reproductive Tract ◽

Intact Female ◽

Periodic Acid Schiff ◽

History Of ◽

Perineal Swelling ◽

Slow Growing

Vaginal cysts are a rare pathology of the female reproductive tract and are usually asymptomatic and often incidental in human and veterinary medicine. A seven-year and 11-month-old intact female bearded collie was referred for further investigations of a two-and-a-half-week history of a slow-growing perineal swelling that was noticed three to four weeks following oestrus. Ultrasound and CT images were acquired, which revealed that an intravaginal cystic mass was the cause of the aforementioned swelling. Following removal of the mass, histopathology revealed a fully excised vaginal cyst lined by simple cuboidal periodic acid-Schiff-positive epithelium most consistent with paramesonephric (Müllerian) origin. To the authors’ knowledge this is the first reported case of an extra-abdominal vaginal wall cyst in a bitch in dioestrus causing perineal swelling.

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